################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## Acc65I AccII AccIII AciI AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BfaI BfrI BglI BglII BlnI BmyI BpmI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FseI FspI HaeII HgaI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MluI MluNI MroI MscI MvaI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 776 GACGTC GACGT^C AccI 94 GTCTAC GT^[AC][GT]AC AcsI 326 AAATTT [GA]^AATT[CT] AcyI 776 GACGTC G[GA]^CG[CT]C AflIII 712 ACATGT A^C[GA][CT]GT ApoI 326 AAATTT [GA]^AATT[CT] BbvCI 151 CCTCAGC CCTCAGC(-5/-2) BcnI 563 CCGGG CC^[GC]GG BlpI 680 GCTCAGC GC^T[ACGT]AGC Bpu1102I 680 GCTCAGC GC^T[ACGT]AGC BsaHI 776 GACGTC G[GA]^CG[CT]C BsaJI 107 CCATGG C^C[ACGT][ACGT]GG BseRI 787 GAGGAG GAGGAG(10/8) BsgI 530 GTGCAG GTGCAG(16/14) BsmI 674 GAATGC GAATGC(1/-1) BspLU11I 712 ACATGT A^CATGT BsrFI 601 GCCGGC [GA]^CCGG[CT] BssKI 563 CCGGG ^CC[ACGT]GG BstYI 534 AGATCC [GA]^GATC[CT] Cac8I 601 GCCGGC GC[ACGT]^[ACGT]GC CelII 680 GCTCAGC GC^T[ACGT]AGC CfoI 678 GCGC GCG^C Cfr10I 601 GCCGGC [GA]^CCGG[CT] DsaI 107 CCATGG C^C[GA][CT]GG EarI 70 CTCTTC CTCTTC(1/4) EcoT14I 107 CCATGG C^C[AT][AT]GG HhaI 678 GCGC GCG^C Hin1I 776 GACGTC G[GA]^CG[CT]C HinP1I 678 GCGC G^CGC Ksp632I 70 CTCTTC CTCTTC(1/4) MaeII 777 ACGT A^CGT MfeI 368 CAATTG C^AATTG MflI 534 AGATCC [GA]^GATC[CT] MspA1I 731 CAGCTG C[AC]G^C[GT]G MunI 368 CAATTG C^AATTG NaeI 601 GCCGGC GCC^GGC NciI 563 CCGGG CC^[GC]GG NcoI 107 CCATGG C^CATGG NgoMIV 601 GCCGGC G^CCGGC NspI 712 ACATGT [GA]CATG^[CT] PshAI 729 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 731 CAGCTG CAG^CTG ScrFI 563 CCGGG CC^[ACGT]GG SfcI 861 CTACAG C^T[GA][CT]AG StuI 701 AGGCCT AGG^CCT StyI 107 CCATGG C^C[AT][AT]GG XhoII 534 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AluI 155 AGCT AG^CT 732 AGCT AlwI 535 GATCC GGATC(4/5) 837 GATCC AvaII 566 GGACC G^G[AT]CC 719 GGTCC BbvI 466 GCAGC GCAGC(8/12) 340 GCTGC BclI 590 TGATCA T^GATCA 905 TGATCA BsiYI 557 CCTGTGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 710 CCACATGTGGG BslI 557 CCTGTGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 710 CCACATGTGGG BsrDI 306 CATTGC GCAATG(2/0) 660 CATTGC BstF5I 654 CATCC GGATG(2/0) 879 GGATG Cfr13I 566 GGACC G^G[ACGT]CC 719 GGTCC DraII 565 GGGACCT [GA]G^G[ACGT]CC[CT] 718 GGGTCCC EcoO109I 565 GGGACCT [GA]G^G[ACGT]CC[CT] 718 GGGTCCC FbaI 590 TGATCA T^GATCA 905 TGATCA Fnu4HI 340 GCTGC GC^[ACGT]GC 466 GCAGC FokI 654 CATCC GGATG(9/13) 879 GGATG HapII 563 CCGG C^CGG 602 CCGG HpaII 563 CCGG C^CGG 602 CCGG ItaI 340 GCTGC GC^[ACGT]GC 466 GCAGC MspI 563 CCGG C^CGG 602 CCGG MwoI 116 GCAATATCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 340 GCTGCGATGGC PleI 459 GAGTC GAGTC(4/5) 790 GAGTC PpuMI 565 GGGACCT [GA]G^G[AT]CC[CT] 718 GGGTCCC Sau96I 566 GGACC G^G[ACGT]CC 719 GGTCC TaqI 54 TCGA T^CGA 420 TCGA TfiI 375 GAATC G^A[AT]TC 849 GAATC TseI 340 GCTGC G^C[AT]GC 466 GCAGC TspRI 292 CAGTG CA[GC]TG[ACGT][ACGT]^ 833 CAGTG TthHB8I 54 TCGA T^CGA 420 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AfaI BsmFI DdeI DpnII HaeIII HinfI HphI MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII NlaIV RsaI Sau3AI Tru9I Tsp45I Tsp509I