################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BciVI BclI BglII BlnI BsaBI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoNI EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccIII 1094 TCCGGA T^CCGGA AflII 414 CTTAAG C^TTAAG BbsI 745 GTCTTC GAAGAC(2/6) BfaI 2344 CTAG C^TAG BfrI 414 CTTAAG C^TTAAG BlpI 2814 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2814 GCTGAGC GC^T[ACGT]AGC BpuAI 745 GTCTTC GAAGAC(2/6) BsaI 862 GAGACC GGTCTC(1/5) BseAI 1094 TCCGGA T^CCGGA BspEI 1094 TCCGGA T^CCGGA BstAPI 2799 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 323 AGATCC [GA]^GATC[CT] CelII 2814 GCTGAGC GC^T[ACGT]AGC DraIII 1647 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 546 GAAGAG CTCTTC(1/4) FseI 2610 GGCCGGCC GGCCGG^CC Ksp632I 546 GAAGAG CTCTTC(1/4) MaeI 2344 CTAG C^TAG MflI 323 AGATCC [GA]^GATC[CT] MroI 1094 TCCGGA T^CCGGA MseI 415 TTAA T^TAA NlaIII 33 CATG CATG^ PaeR7I 2068 CTCGAG C^TCGAG SacI 841 GAGCTC GAGCT^C SapI 546 GAAGAGC GCTCTTC(1/4) SgrAI 510 CACCGGCG C[GA]^CCGG[CT]G TfiI 908 GATTC G^A[AT]TC Tru9I 415 TTAA T^TAA Tsp509I 2626 AATT ^AATT XhoI 2068 CTCGAG C^TCGAG XhoII 323 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 1646 ACACGT A^C[GA][CT]GT 3170 ACACGT AlwI 324 GATCC GGATC(4/5) 2169 GATCC AocI 1920 CCTGAGG CC^T[ACGT]AGG 3112 CCTCAGG Asp700I 546 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2863 GAAACACTTC BbvCI 1261 GCTGAGG CCTCAGC(-5/-2) 2689 GCTGAGG BcgI 740 GCAAGGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1715 GCAAGAGCTTCG BsaWI 134 TCCGGT [AT]^CCGG[AT] 1094 TCCGGA BsmAI 862 GAGAC GTCTC(1/5) 2773 GAGAC BstXI 1196 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2660 CCAACCTGCTGG Bsu36I 1920 CCTGAGG CC^T[ACGT]AGG 3112 CCTCAGG DpnII 324 GATC ^GATC 2169 GATC EagI 1238 CGGCCG C^GGCCG 2262 CGGCCG EclXI 1238 CGGCCG C^GGCCG 2262 CGGCCG Eco52I 1238 CGGCCG C^GGCCG 2262 CGGCCG Eco81I 1920 CCTGAGG CC^T[ACGT]AGG 3112 CCTCAGG MboI 324 GATC ^GATC 2169 GATC MboII 546 GAAGA GAAGA(8/7) 746 TCTTC MslI 131 CATTCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2745 CACTGGTGTG NdeII 324 GATC ^GATC 2169 GATC PflMI 2329 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2601 CCAGCCCGTGG PshAI 2520 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2586 GACCACGGTC Sau3AI 324 GATC ^GATC 2169 GATC SfaNI 958 GATGC GCATC(5/9) 1209 GCATC SfiI 367 GGCCAGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1537 GGCCAGTGCGGCC SmlI 414 CTTAAG C^T[CT][GA]AG 2068 CTCGAG TaqI 2069 TCGA T^CGA 2841 TCGA TthHB8I 2069 TCGA T^CGA 2841 TCGA Van91I 2329 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2601 CCAGCCCGTGG XmnI 546 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2863 GAAACACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwNI Aor51HI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbvI BcnI BglI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI Eco47III EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MaeIII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIV PleI PmaCI PmlI PstI PvuII RsaI SacII Sau96I ScrFI SfcI SfoI SmaI StuI StyI TseI Tsp45I TspRI XcmI XmaCI XmaI