(Circular) (Six-base) MAPSORT of: pFN21AE5830.seq Check: 9352 from: 1 to: 7882 pFN21AE5830 7882 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3429 3597 3881 3429 Size: 168 284 7430 Fragments arranged by size: 7430 284 168 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5692 278 Size: 53 83 186 936 4156 2468 Fragments arranged by size: 4156 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5225 1937 Size: 3288 4594 Acc65I G'GTAC_C Cuts at: 5203 5203 Size: 7882 AclI AA'CG_TT Cuts at: 1563 6009 6382 1563 Size: 4446 373 3063 Fragments arranged by size: 4446 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5945 7329 7597 1632 Size: 4313 1384 268 1917 Fragments arranged by size: 4313 1917 1384 268 AfeI AGC'GCT Cuts at: 2312 3482 4372 2312 Size: 1170 890 5822 Fragments arranged by size: 5822 1170 890 AflII C'TTAA_G Cuts at: 820 1017 2419 820 Size: 197 1402 6283 Fragments arranged by size: 6283 1402 197 AflIII A'CryG_T Cuts at: 1203 3651 5175 6781 7758 1203 Size: 2448 1524 1606 977 1327 Fragments arranged by size: 2448 1606 1524 1327 977 AgeI A'CCGG_T Cuts at: 6713 6713 Size: 7882 AhdI GACnn_n'nnGTC Cuts at: 6611 6611 Size: 7882 AleI CACnn'nnGTG Cuts at: 1166 4754 1166 Size: 3588 4294 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2591 1184 Size: 1407 6475 AlwNI CAG_nnn'CTG Cuts at: 2084 3971 4340 4952 5005 5096 5125 5625 7197 Size: 1887 369 612 53 91 29 500 1572 Cuts at: 7197 2084 Size: 2769 Fragments arranged by size: 2769 1887 1572 612 500 369 91 53 29 ApaI G_GGCC'C Cuts at: 2961 4018 4286 5039 2961 Size: 1057 268 753 5804 Fragments arranged by size: 5804 1057 753 268 ApaLI G'TGCA_C Cuts at: 2342 2510 2594 3512 3764 5171 5939 7095 2342 Size: 168 84 918 252 1407 768 1156 3129 Fragments arranged by size: 3129 1407 1156 918 768 252 168 84 ApoI r'AATT_y Cuts at: 1493 5191 5417 5569 7678 1493 Size: 3698 226 152 2109 1697 Fragments arranged by size: 3698 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6436 160 Size: 872 5404 1606 Fragments arranged by size: 5404 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7882 AvaI C'yCGr_G Cuts at: 1870 1943 2698 2733 2954 4073 4102 4721 5032 Size: 73 755 35 221 1119 29 619 311 Cuts at: 5032 5051 5207 1870 Size: 19 156 4545 Fragments arranged by size: 4545 1119 755 619 311 221 156 73 35 29 19 BamHI G'GATC_C Cuts at: 5212 5579 5212 Size: 367 7515 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2496 2684 2702 2778 Size: 325 217 627 254 455 188 18 76 Cuts at: 2778 2839 2968 2986 2998 3249 3304 3921 4660 Size: 61 129 18 12 251 55 617 739 Cuts at: 4660 4985 5203 6658 618 Size: 325 218 1455 1842 Fragments arranged by size: 1842 1455 739 627 617 455 325 325 254 251 218 217 188 129 76 61 55 18 18 12 BanII G_rGCy'C Cuts at: 1391 2742 2850 2961 3117 3401 4018 4286 5039 Size: 1351 108 111 156 284 617 268 753 Cuts at: 5039 5049 5059 5201 1391 Size: 10 10 142 4072 Fragments arranged by size: 4072 1351 753 617 284 268 156 142 111 108 10 10 BbeI G_GCGC'C Cuts at: 2045 2500 2688 2972 3002 3253 3308 2045 Size: 455 188 284 30 251 55 6619 Fragments arranged by size: 6619 455 284 251 188 55 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2743 928 Size: 971 844 6067 Fragments arranged by size: 6067 971 844 BbvCI CC'TCA_GC Cuts at: 3267 4695 3267 Size: 1428 6454 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2734 2768 3709 3743 6073 6107 1903 Size: 34 797 34 941 34 2330 34 3678 Fragments arranged by size: 3678 2330 941 797 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5774 6984 5774 Size: 1210 6672 BclI T'GATC_A Cuts at: 1548 1548 Size: 7882 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 2265 2379 Size: 107 122 71 631 605 430 163 114 Cuts at: 2379 2714 3549 6493 136 Size: 335 835 2944 1525 Fragments arranged by size: 2944 1525 835 631 605 430 335 163 122 114 107 71 BglII A'GATC_T Cuts at: 7877 7877 Size: 7882 BlpI GC'TnA_GC Cuts at: 4820 5299 4820 Size: 479 7403 Bme1580I G_kGCm'C Cuts at: 1790 2204 2288 2346 2456 2514 2598 2954 2961 Size: 414 84 58 110 58 84 356 7 Cuts at: 2961 3516 3768 4018 4091 4286 5039 5175 5943 Size: 555 252 250 73 195 753 136 768 Cuts at: 5943 7099 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 768 753 555 414 356 252 250 195 136 110 84 84 73 58 58 7 BmgBI CAC'GTC Cuts at: 1318 3654 4042 1318 Size: 2336 388 5158 Fragments arranged by size: 5158 2336 388 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2101 2722 5613 Size: 416 53 83 399 24 684 621 2891 Cuts at: 5613 6571 442 Size: 958 1753 Fragments arranged by size: 2891 1753 958 684 621 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7882 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2768 2957 4040 4151 4231 4448 4490 4523 Size: 1529 189 1083 111 80 217 42 33 Cuts at: 4523 4556 4589 4769 4998 5043 6542 1239 Size: 33 33 180 229 45 1499 2579 Fragments arranged by size: 2579 1529 1499 1083 229 217 189 180 111 80 45 42 33 33 33 Bpu10I CC'TnA_GC Cuts at: 2844 3267 4695 2844 Size: 423 1428 6031 Fragments arranged by size: 6031 1428 423 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6007 6872 7170 6007 Size: 865 298 6719 Fragments arranged by size: 6719 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2861 6545 882 Size: 617 1362 3684 2219 Fragments arranged by size: 3684 2219 1362 617 BsaAI yAC'GTr Cuts at: 493 1204 2509 3229 3679 5673 493 Size: 711 1305 720 450 1994 2702 Fragments arranged by size: 2702 1994 1305 720 711 450 BsaBI GATnn'nnATC Cuts at: 7876 7876 Size: 7882 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 2497 2685 2763 Size: 53 83 186 936 509 455 188 78 Cuts at: 2763 2969 2999 3250 3305 5689 6071 6706 275 Size: 206 30 251 55 2384 382 635 1451 Fragments arranged by size: 2384 1451 936 635 509 455 382 251 206 188 186 83 78 55 53 30 BsaWI w'CCGG_w Cuts at: 2139 3099 6315 6713 6987 7134 7545 2139 Size: 960 3216 398 274 147 411 2476 Fragments arranged by size: 3216 2476 960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2870 2900 4861 4891 4908 Size: 30 165 30 1467 30 1961 30 17 Cuts at: 4908 4938 1178 Size: 30 4122 Fragments arranged by size: 4122 1961 1467 165 30 30 30 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2221 2305 2641 2939 3033 3248 3391 3475 Size: 1035 84 336 298 94 215 143 84 Cuts at: 3475 3811 4234 4712 4916 4995 1186 Size: 336 423 478 204 79 4073 Fragments arranged by size: 4073 1035 478 423 336 336 298 215 204 143 94 84 84 79 BseYI C'CCAG_C Cuts at: 2973 3110 3956 3984 4178 4253 4259 4373 4945 Size: 137 846 28 194 75 6 114 572 Cuts at: 4945 5026 5096 7085 7581 2973 Size: 81 70 1989 496 3274 Fragments arranged by size: 3274 1989 846 572 496 194 137 114 81 75 70 28 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 2651 3581 3821 4208 4376 4478 Size: 573 408 507 930 240 387 168 102 Cuts at: 4478 1163 Size: 4567 Fragments arranged by size: 4567 930 573 507 408 387 240 168 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3246 3889 4270 5332 6093 7121 Size: 275 186 1245 643 381 1062 761 1028 Cuts at: 7121 1540 Size: 2301 Fragments arranged by size: 2301 1245 1062 1028 761 643 381 275 186 BsiHKAI G_wGCw'C Cuts at: 2346 2514 2570 2598 2850 2943 3516 3768 5175 Size: 168 56 28 252 93 573 252 1407 Cuts at: 5175 5943 6028 7099 2346 Size: 768 85 1071 3129 Fragments arranged by size: 3129 1407 1071 768 573 252 252 168 93 85 56 28 BsmI GAATG_Cn' Cuts at: 5393 5486 5393 Size: 93 7789 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 2288 2346 2456 2514 2570 2598 Size: 399 414 84 58 110 58 56 28 Cuts at: 2598 2742 2850 2943 2954 2961 3117 3401 3516 Size: 144 108 93 11 7 156 284 115 Cuts at: 3516 3768 4018 4091 4286 5039 5049 5059 5175 Size: 252 250 73 195 753 10 10 116 Cuts at: 5175 5201 5943 6028 7099 1391 Size: 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 753 742 414 399 284 252 250 195 156 144 116 115 110 108 93 85 84 73 58 58 56 28 26 11 10 10 7 BspEI T'CCGG_A Cuts at: 3099 7545 3099 Size: 4446 3436 BspHI T'CATG_A Cuts at: 5771 7501 5771 Size: 1730 6152 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2583 2667 2778 3293 3429 3597 3753 3837 Size: 1739 84 111 515 136 168 156 84 Cuts at: 3837 3881 4677 5237 844 Size: 44 796 560 3489 Fragments arranged by size: 3489 1739 796 560 515 168 156 136 111 84 84 44 BsrBI CCG'CTC Cuts at: 1394 1823 2188 2272 2524 2608 2626 3358 3442 Size: 429 365 84 252 84 18 732 84 Cuts at: 3442 3610 3778 3796 4364 4686 4824 5769 1394 Size: 168 168 18 568 322 138 945 3507 Fragments arranged by size: 3507 945 732 568 429 365 322 252 168 168 138 84 84 84 18 18 BsrDI GCAATG_nn' Cuts at: 65 1248 6377 6551 65 Size: 1183 5129 174 1396 Fragments arranged by size: 5129 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 2348 2516 3146 3977 4616 4904 Size: 110 234 304 168 630 831 639 288 Cuts at: 4904 6526 6713 1700 Size: 1622 187 2869 Fragments arranged by size: 2869 1622 831 639 630 304 288 234 187 168 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7882 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7882 BssSI C'ACGA_G Cuts at: 5942 6954 5942 Size: 1012 6870 BstAPI GCAn_nnn'nTGC Cuts at: 794 4810 794 Size: 4016 3866 BstBI TT'CG_AA Cuts at: 6686 6686 Size: 7882 BstEII G'GTnAC_C Cuts at: 1184 3280 4522 4588 1184 Size: 2096 1242 66 4478 Fragments arranged by size: 4478 2096 1242 66 BstXI CCAn_nnnn'nTGG Cuts at: 3208 4672 3208 Size: 1464 6418 BstYI r'GATC_y Cuts at: 1972 2328 5212 5579 5964 5981 7433 7519 7531 Size: 356 2884 367 385 17 1452 86 12 Cuts at: 7531 7877 1972 Size: 346 1977 Fragments arranged by size: 2884 1977 1452 385 367 356 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3926 5118 3926 Size: 1192 6690 BtgI C'CryG_G Cuts at: 513 1065 2712 2835 2871 3136 3240 4286 4494 Size: 552 1647 123 36 265 104 1046 208 Cuts at: 4494 4560 4593 4611 5040 513 Size: 66 33 18 429 3355 Fragments arranged by size: 3355 1647 1046 552 429 265 208 123 104 66 36 33 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2910 487 Size: 683 56 1684 5459 Fragments arranged by size: 5459 1684 683 56 BtsI GCAGTG_nn' Cuts at: 3227 4299 5398 6192 6212 3227 Size: 1072 1099 794 20 4897 Fragments arranged by size: 4897 1099 1072 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7882 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4618 4653 529 Size: 35 4054 35 3758 Fragments arranged by size: 4054 3758 35 35 DraI TTT'AAA Cuts at: 5186 5186 Size: 7882 DraIII CAC_nnn'GTG Cuts at: 3657 3657 Size: 7882 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6889 809 Size: 507 5573 1802 Fragments arranged by size: 5573 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2371 3198 3243 Size: 54 1425 50 275 18 541 827 45 Cuts at: 3243 3495 3541 3903 3942 4267 4365 4614 4618 Size: 252 46 362 39 325 98 249 4 Cuts at: 4618 5329 6218 7847 8 Size: 711 889 1629 43 Fragments arranged by size: 1629 1425 889 827 711 541 362 325 275 252 249 98 54 50 46 45 43 39 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3243 4267 5329 1537 Size: 275 1431 1024 1062 4090 Fragments arranged by size: 4090 1431 1062 1024 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2546 5812 1353 Size: 1193 3266 3423 Fragments arranged by size: 3423 3266 1193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2879 3328 3872 4194 6469 6843 Size: 1064 138 1290 449 544 322 2275 374 Cuts at: 6843 6989 387 Size: 146 1280 Fragments arranged by size: 2275 1290 1280 1064 544 449 374 322 146 138 EcoICRI GAG'CTC Cuts at: 2848 2848 Size: 7882 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2768 2957 4040 4151 4231 4448 4490 Size: 393 1136 189 1083 111 80 217 42 Cuts at: 4490 4523 4556 4589 4769 4998 5043 5945 6542 Size: 33 33 33 180 229 45 902 597 Cuts at: 6542 7329 7597 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1136 1083 902 787 597 393 268 229 217 189 180 111 80 45 42 33 33 33 EcoO109I rG'GnC_Cy Cuts at: 2081 3696 4015 4099 4162 4282 4337 4718 5036 Size: 1615 319 84 63 120 55 381 318 Cuts at: 5036 5122 2081 Size: 86 4841 Fragments arranged by size: 4841 1615 381 319 318 120 86 84 63 55 EcoRI G'AATT_C Cuts at: 5191 5569 5191 Size: 378 7504 FseI GG_CCGG'CC Cuts at: 4620 4620 Size: 7882 FspI TGC'GCA Cuts at: 6388 6388 Size: 7882 HaeII r_GCGC'y Cuts at: 1678 2045 2314 2443 2500 2688 2972 3002 3253 Size: 367 269 129 57 188 284 30 251 Cuts at: 3253 3308 3484 3865 4124 4374 4944 7029 1678 Size: 55 176 381 259 250 570 2085 2531 Fragments arranged by size: 2531 2085 570 381 367 284 269 259 251 250 188 176 129 57 55 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4908 4940 6523 6555 6597 Size: 32 135 32 3336 32 1583 32 42 Cuts at: 6597 6629 1373 Size: 32 2626 Fragments arranged by size: 3336 2626 1583 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5226 5472 669 Size: 988 281 3288 246 3079 Fragments arranged by size: 3288 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7882 HpaI GTT'AAC Cuts at: 5472 5472 Size: 7882 KasI G'GCGC_C Cuts at: 2041 2496 2684 2968 2998 3249 3304 2041 Size: 455 188 284 30 251 55 6619 Fragments arranged by size: 6619 455 284 251 188 55 30 KpnI G_GTAC'C Cuts at: 5207 5207 Size: 7882 MfeI C'AATT_G Cuts at: 5481 5481 Size: 7882 MluI A'CGCG_T Cuts at: 7758 7758 Size: 7882 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2679 3765 3849 4498 6996 7180 1305 Size: 1374 1086 84 649 2498 184 2007 Fragments arranged by size: 2498 2007 1374 1086 649 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 3200 3497 3543 10 Size: 54 1425 343 1368 297 46 4349 Fragments arranged by size: 4349 1425 1368 343 297 54 46 MslI CAynn'nnrTG Cuts at: 518 1166 2140 4754 5840 6199 6358 518 Size: 648 974 2614 1086 359 159 2042 Fragments arranged by size: 2614 2042 1086 974 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2619 2714 2776 2892 3165 3174 3196 3242 Size: 1013 95 62 116 273 9 22 46 Cuts at: 3242 3301 3508 3789 3919 4171 4288 4383 4694 Size: 59 207 281 130 252 117 95 311 Cuts at: 4694 5042 5167 5298 5975 7123 7368 1606 Size: 348 125 131 677 1148 245 2120 Fragments arranged by size: 2120 1148 1013 677 348 311 281 273 252 245 207 131 130 125 117 116 95 95 62 59 46 22 9 NaeI GCC'GGC Cuts at: 2046 3148 3979 4618 2046 Size: 1102 831 639 5310 Fragments arranged by size: 5310 1102 831 639 NarI GG'CG_CC Cuts at: 2042 2497 2685 2969 2999 3250 3305 2042 Size: 455 188 284 30 251 55 6619 Fragments arranged by size: 6619 455 284 251 188 55 30 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7330 NdeI CA'TA_TG Cuts at: 387 387 Size: 7882 NgoMIV G'CCGG_C Cuts at: 2044 3146 3977 4616 2044 Size: 1102 831 639 5310 Fragments arranged by size: 5310 1102 831 639 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7882 NotI GC'GGCC_GC Cuts at: 5329 5329 Size: 7882 NspI r_CATG'y Cuts at: 1132 1229 5240 6785 1132 Size: 97 4011 1545 2229 Fragments arranged by size: 4011 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4159 4340 1348 Size: 2811 181 4890 Fragments arranged by size: 4890 2811 181 PciI A'CATG_T Cuts at: 6781 6781 Size: 7882 PflMI CCAn_nnn'nTGG Cuts at: 1108 4340 4612 1108 Size: 3232 272 4378 Fragments arranged by size: 4378 3232 272 PfoI T'CCnGG_A Cuts at: 2934 4761 2934 Size: 1827 6055 PmeI GTTT'AAAC Cuts at: 5186 5186 Size: 7882 PmlI CAC'GTG Cuts at: 2509 3229 3679 2509 Size: 720 450 6712 Fragments arranged by size: 6712 720 450 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5951 5983 7464 7496 5951 Size: 32 1481 32 6337 Fragments arranged by size: 6337 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4529 4595 1613 Size: 2916 66 4900 Fragments arranged by size: 4900 2916 66 PsiI TTA'TAA Cuts at: 5452 5452 Size: 7882 PspOMI G'GGCC_C Cuts at: 2957 4014 4282 5035 2957 Size: 1057 268 753 5804 Fragments arranged by size: 5804 1057 753 268 PspXI vC'TCGA_Gb Cuts at: 4073 4073 Size: 7882 PstI C_TGCA'G Cuts at: 830 2328 2775 3918 4413 5001 5234 830 Size: 1498 447 1143 495 588 233 3478 Fragments arranged by size: 3478 1498 1143 588 495 447 233 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7882 PvuII CAG'CTG Cuts at: 3174 3196 4171 4694 5167 3174 Size: 22 975 523 473 5889 Fragments arranged by size: 5889 975 523 473 22 SacI G_AGCT'C Cuts at: 2850 2850 Size: 7882 SacII CC_GC'GG Cuts at: 2715 3243 4289 5043 2715 Size: 528 1046 754 5554 Fragments arranged by size: 5554 1046 754 528 SalI G'TCGA_C Cuts at: 1936 5224 1936 Size: 3288 4594 SapI GCTCTTCn'nnn_ Cuts at: 2546 2546 Size: 7882 SbfI CC_TGCA'GG Cuts at: 5234 5234 Size: 7882 ScaI AGT'ACT Cuts at: 6130 7842 6130 Size: 1712 6170 SfcI C'TryA_G Cuts at: 826 1045 2324 2771 3914 4409 4997 5230 6365 Size: 219 1279 447 1143 495 588 233 1135 Cuts at: 6365 7046 7237 826 Size: 681 191 1471 Fragments arranged by size: 1471 1279 1143 1135 681 588 495 447 233 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2379 3549 2379 Size: 1170 6712 SfoI GGC'GCC Cuts at: 2043 2498 2686 2970 3000 3251 3306 2043 Size: 455 188 284 30 251 55 6619 Fragments arranged by size: 6619 455 284 251 188 55 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7882 SgrAI Cr'CCGG_yG Cuts at: 1700 2516 1700 Size: 816 7066 SmaI CCC'GGG Cuts at: 1872 2700 2735 2956 4723 5053 5209 1872 Size: 828 35 221 1767 330 156 4545 Fragments arranged by size: 4545 1767 828 330 221 156 35 SmlI C'TyrA_G Cuts at: 820 1017 1943 2419 4073 5986 6887 7149 820 Size: 197 926 476 1654 1913 901 262 1553 Fragments arranged by size: 1913 1654 1553 926 901 476 262 197 SnaBI TAC'GTA Cuts at: 493 5673 493 Size: 5180 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7882 SphI G_CATG'C Cuts at: 1132 5240 1132 Size: 4108 3774 SspI AAT'ATT Cuts at: 5 52 5806 5 Size: 47 5754 2081 Fragments arranged by size: 5754 2081 47 StuI AGG'CCT Cuts at: 1517 2303 2471 3473 3641 1517 Size: 786 168 1002 168 5758 Fragments arranged by size: 5758 1002 786 168 168 StyI C'CwwG_G Cuts at: 513 1065 2214 2298 2466 3003 5321 513 Size: 552 1149 84 168 537 2318 3074 Fragments arranged by size: 3074 2318 1149 552 537 168 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2660 3830 4043 4678 5909 5926 6079 Size: 1255 1080 1170 213 635 1231 17 153 Cuts at: 6079 6264 6709 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1231 1170 1080 635 445 213 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6128 7840 96 Size: 274 80 33 51 1443 4151 1712 138 Fragments arranged by size: 4151 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7842 7874 7842 Size: 32 7850 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7882 XbaI T'CTAG_A Cuts at: 5218 5218 Size: 7882 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2114 2878 3683 4753 4957 2114 Size: 764 805 1070 204 5039 Fragments arranged by size: 5039 1070 805 764 204 XhoI C'TCGA_G Cuts at: 1943 4073 1943 Size: 2130 5752 XmaI C'CCGG_G Cuts at: 1870 2698 2733 2954 4721 5051 5207 1870 Size: 828 35 221 1767 330 156 4545 Fragments arranged by size: 4545 1767 828 330 221 156 35 XmnI GAAnn'nnTTC Cuts at: 2555 4872 6011 2555 Size: 2317 1139 4426 Fragments arranged by size: 4426 2317 1139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5690 276 Size: 53 83 186 936 4156 2468 Fragments arranged by size: 4156 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FspAI NruI NsiI PacI PpuMI PsrI RsrII SanDI SexAI SrfI SwaI