(Linear) (Six-base) MAPSORT of: KIEE5830.seq Check: 119 from: 1 to: 3177 KIEE5830.orf With 182 enzymes: SgfI * December 5, 2014 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1424 1592 1876 3177 Size: 1424 168 284 1301 Fragments arranged by size: 1424 1301 284 168 AfeI AGC'GCT Cuts at: 0 307 1477 2367 3177 Size: 307 1170 890 810 Fragments arranged by size: 1170 890 810 307 AflII C'TTAA_G Cuts at: 0 414 3177 Size: 414 2763 AflIII A'CryG_T Cuts at: 0 1646 3170 3177 Size: 1646 1524 7 Fragments arranged by size: 1646 1524 7 AleI CACnn'nnGTG Cuts at: 0 2749 3177 Size: 2749 428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 586 3177 Size: 586 2591 AlwNI CAG_nnn'CTG Cuts at: 0 79 1966 2335 2947 3000 3091 3120 3177 Size: 79 1887 369 612 53 91 29 57 Fragments arranged by size: 1887 612 369 91 79 57 53 29 ApaI G_GGCC'C Cuts at: 0 956 2013 2281 3034 3177 Size: 956 1057 268 753 143 Fragments arranged by size: 1057 956 753 268 143 ApaLI G'TGCA_C Cuts at: 0 337 505 589 1507 1759 3166 3177 Size: 337 168 84 918 252 1407 11 Fragments arranged by size: 1407 918 337 252 168 84 11 AvaI C'yCGr_G Cuts at: 0 693 728 949 2068 2097 2716 3027 3046 Size: 693 35 221 1119 29 619 311 19 Cuts at: 3046 3177 Size: 131 Fragments arranged by size: 1119 693 619 311 221 131 35 29 19 BanI G'GyrC_C Cuts at: 0 36 491 679 697 773 834 963 981 Size: 36 455 188 18 76 61 129 18 Cuts at: 981 993 1244 1299 1916 2655 2980 3177 Size: 12 251 55 617 739 325 197 Fragments arranged by size: 739 617 455 325 251 197 188 129 76 61 55 36 18 18 12 BanII G_rGCy'C Cuts at: 0 737 845 956 1112 1396 2013 2281 3034 Size: 737 108 111 156 284 617 268 753 Cuts at: 3034 3044 3054 3177 Size: 10 10 123 Fragments arranged by size: 753 737 617 284 268 156 123 111 108 10 10 BbeI G_GCGC'C Cuts at: 0 40 495 683 967 997 1248 1303 3177 Size: 40 455 188 284 30 251 55 1874 Fragments arranged by size: 1874 455 284 251 188 55 40 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 738 3177 Size: 738 2439 BbvCI CC'TCA_GC Cuts at: 0 1262 2690 3177 Size: 1262 1428 487 Fragments arranged by size: 1428 1262 487 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 729 763 1704 1738 3177 Size: 729 34 941 34 1439 Fragments arranged by size: 1439 941 729 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 97 260 374 709 1544 3177 Size: 97 163 114 335 835 1633 Fragments arranged by size: 1633 835 335 163 114 97 BlpI GC'TnA_GC Cuts at: 0 2815 3177 Size: 2815 362 Bme1580I G_kGCm'C Cuts at: 0 199 283 341 451 509 593 949 956 Size: 199 84 58 110 58 84 356 7 Cuts at: 956 1511 1763 2013 2086 2281 3034 3170 3177 Size: 555 252 250 73 195 753 136 7 Fragments arranged by size: 753 555 356 252 250 199 195 136 110 84 84 73 58 58 7 7 BmgBI CAC'GTC Cuts at: 0 1649 2037 3177 Size: 1649 388 1140 Fragments arranged by size: 1649 1140 388 BmrI ACTGGGnnnn_n' Cuts at: 0 96 717 3177 Size: 96 621 2460 Fragments arranged by size: 2460 621 96 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 763 952 2035 2146 2226 2443 2485 2518 Size: 763 189 1083 111 80 217 42 33 Cuts at: 2518 2551 2584 2764 2993 3038 3177 Size: 33 33 180 229 45 139 Fragments arranged by size: 1083 763 229 217 189 180 139 111 80 45 42 33 33 33 Bpu10I CC'TnA_GC Cuts at: 0 839 1262 2690 3177 Size: 839 423 1428 487 Fragments arranged by size: 1428 839 487 423 BsaI GGTCTCn'nnnn_ Cuts at: 0 856 3177 Size: 856 2321 BsaAI yAC'GTr Cuts at: 0 504 1224 1674 3177 Size: 504 720 450 1503 Fragments arranged by size: 1503 720 504 450 BsaHI Gr'CG_yC Cuts at: 0 37 492 680 758 964 994 1245 1300 Size: 37 455 188 78 206 30 251 55 Cuts at: 1300 3177 Size: 1877 Fragments arranged by size: 1877 455 251 206 188 78 55 37 30 BsaWI w'CCGG_w Cuts at: 0 134 1094 3177 Size: 134 960 2083 Fragments arranged by size: 2083 960 134 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 865 895 2856 2886 2903 2933 3177 Size: 865 30 1961 30 17 30 244 Fragments arranged by size: 1961 865 244 30 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 216 300 636 934 1028 1243 1386 1470 Size: 216 84 336 298 94 215 143 84 Cuts at: 1470 1806 2229 2707 2911 2990 3177 Size: 336 423 478 204 79 187 Fragments arranged by size: 478 423 336 336 298 216 215 204 187 143 94 84 84 79 BseYI C'CCAG_C Cuts at: 0 968 1105 1951 1979 2173 2248 2254 2368 Size: 968 137 846 28 194 75 6 114 Cuts at: 2368 2940 3021 3091 3177 Size: 572 81 70 86 Fragments arranged by size: 968 846 572 194 137 114 86 81 75 70 28 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 646 1576 1816 2203 2371 2473 3177 Size: 139 507 930 240 387 168 102 704 Fragments arranged by size: 930 704 507 387 240 168 139 102 BsiEI CG_ry'CG Cuts at: 0 1241 1884 2265 3177 Size: 1241 643 381 912 Fragments arranged by size: 1241 912 643 381 BsiHKAI G_wGCw'C Cuts at: 0 341 509 565 593 845 938 1511 1763 Size: 341 168 56 28 252 93 573 252 Cuts at: 1763 3170 3177 Size: 1407 7 Fragments arranged by size: 1407 573 341 252 252 168 93 56 28 7 Bsp1286I G_dGCh'C Cuts at: 0 199 283 341 451 509 565 593 737 Size: 199 84 58 110 58 56 28 144 Cuts at: 737 845 938 949 956 1112 1396 1511 1763 Size: 108 93 11 7 156 284 115 252 Cuts at: 1763 2013 2086 2281 3034 3044 3054 3170 3177 Size: 250 73 195 753 10 10 116 7 Fragments arranged by size: 753 284 252 250 199 195 156 144 116 115 110 108 93 84 73 58 58 56 28 11 10 10 7 7 BspEI T'CCGG_A Cuts at: 0 1094 3177 Size: 1094 2083 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 578 662 773 1288 1424 1592 1748 1832 Size: 578 84 111 515 136 168 156 84 Cuts at: 1832 1876 2672 3177 Size: 44 796 505 Fragments arranged by size: 796 578 515 505 168 156 136 111 84 84 44 BsrBI CCG'CTC Cuts at: 0 183 267 519 603 621 1353 1437 1605 Size: 183 84 252 84 18 732 84 168 Cuts at: 1605 1773 1791 2359 2681 2819 3177 Size: 168 18 568 322 138 358 Fragments arranged by size: 732 568 358 322 252 183 168 168 138 84 84 84 18 18 BsrFI r'CCGG_y Cuts at: 0 39 343 511 1141 1972 2611 2899 3177 Size: 39 304 168 630 831 639 288 278 Fragments arranged by size: 831 639 630 304 288 278 168 39 BstAPI GCAn_nnn'nTGC Cuts at: 0 2805 3177 Size: 2805 372 BstEII G'GTnAC_C Cuts at: 0 1275 2517 2583 3177 Size: 1275 1242 66 594 Fragments arranged by size: 1275 1242 594 66 BstXI CCAn_nnnn'nTGG Cuts at: 0 1203 2667 3177 Size: 1203 1464 510 Fragments arranged by size: 1464 1203 510 BstYI r'GATC_y Cuts at: 0 323 3177 Size: 323 2854 Bsu36I CC'TnA_GG Cuts at: 0 1921 3113 3177 Size: 1921 1192 64 Fragments arranged by size: 1921 1192 64 BtgI C'CryG_G Cuts at: 0 707 830 866 1131 1235 2281 2489 2555 Size: 707 123 36 265 104 1046 208 66 Cuts at: 2555 2588 2606 3035 3177 Size: 33 18 429 142 Fragments arranged by size: 1046 707 429 265 208 142 123 104 66 36 33 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 905 3177 Size: 905 2272 BtsI GCAGTG_nn' Cuts at: 0 1222 2294 3177 Size: 1222 1072 883 Fragments arranged by size: 1222 1072 883 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2613 2648 3177 Size: 2613 35 529 Fragments arranged by size: 2613 529 35 DraIII CAC_nnn'GTG Cuts at: 0 1652 3177 Size: 1652 1525 EaeI y'GGCC_r Cuts at: 0 366 1193 1238 1490 1536 1898 1937 2262 Size: 366 827 45 252 46 362 39 325 Cuts at: 2262 2360 2609 2613 3177 Size: 98 249 4 564 Fragments arranged by size: 827 564 366 362 325 252 249 98 46 45 39 4 EagI C'GGCC_G Cuts at: 0 1238 2262 3177 Size: 1238 1024 915 Fragments arranged by size: 1238 1024 915 EarI CTCTTCn'nnn_ Cuts at: 0 541 3177 Size: 541 2636 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 874 1323 1867 2189 3177 Size: 874 449 544 322 988 Fragments arranged by size: 988 874 544 449 322 EcoICRI GAG'CTC Cuts at: 0 843 3177 Size: 843 2334 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 763 952 2035 2146 2226 2443 2485 2518 Size: 763 189 1083 111 80 217 42 33 Cuts at: 2518 2551 2584 2764 2993 3038 3177 Size: 33 33 180 229 45 139 Fragments arranged by size: 1083 763 229 217 189 180 139 111 80 45 42 33 33 33 EcoO109I rG'GnC_Cy Cuts at: 0 76 1691 2010 2094 2157 2277 2332 2713 Size: 76 1615 319 84 63 120 55 381 Cuts at: 2713 3031 3117 3177 Size: 318 86 60 Fragments arranged by size: 1615 381 319 318 120 86 84 76 63 60 55 FseI GG_CCGG'CC Cuts at: 0 2615 3177 Size: 2615 562 HaeII r_GCGC'y Cuts at: 0 40 309 438 495 683 967 997 1248 Size: 40 269 129 57 188 284 30 251 Cuts at: 1248 1303 1479 1860 2119 2369 2939 3177 Size: 55 176 381 259 250 570 238 Fragments arranged by size: 570 381 284 269 259 251 250 238 188 176 129 57 55 40 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2903 2935 3177 Size: 2903 32 242 Fragments arranged by size: 2903 242 32 KasI G'GCGC_C Cuts at: 0 36 491 679 963 993 1244 1299 3177 Size: 36 455 188 284 30 251 55 1878 Fragments arranged by size: 1878 455 284 251 188 55 36 30 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 674 1760 1844 2493 3177 Size: 674 1086 84 649 684 Fragments arranged by size: 1086 684 674 649 84 MscI TGG'CCA Cuts at: 0 1195 1492 1538 3177 Size: 1195 297 46 1639 Fragments arranged by size: 1639 1195 297 46 MslI CAynn'nnrTG Cuts at: 0 135 2749 3177 Size: 135 2614 428 Fragments arranged by size: 2614 428 135 MspA1I CmG'CkG Cuts at: 0 614 709 771 887 1160 1169 1191 1237 Size: 614 95 62 116 273 9 22 46 Cuts at: 1237 1296 1503 1784 1914 2166 2283 2378 2689 Size: 59 207 281 130 252 117 95 311 Cuts at: 2689 3037 3162 3177 Size: 348 125 15 Fragments arranged by size: 614 348 311 281 273 252 207 130 125 117 116 95 95 62 59 46 22 15 9 NaeI GCC'GGC Cuts at: 0 41 1143 1974 2613 3177 Size: 41 1102 831 639 564 Fragments arranged by size: 1102 831 639 564 41 NarI GG'CG_CC Cuts at: 0 37 492 680 964 994 1245 1300 3177 Size: 37 455 188 284 30 251 55 1877 Fragments arranged by size: 1877 455 284 251 188 55 37 30 NgoMIV G'CCGG_C Cuts at: 0 39 1141 1972 2611 3177 Size: 39 1102 831 639 566 Fragments arranged by size: 1102 831 639 566 39 PasI CC'CwG_GG Cuts at: 0 2154 2335 3177 Size: 2154 181 842 Fragments arranged by size: 2154 842 181 PflMI CCAn_nnn'nTGG Cuts at: 0 2335 2607 3177 Size: 2335 272 570 Fragments arranged by size: 2335 570 272 PfoI T'CCnGG_A Cuts at: 0 929 2756 3177 Size: 929 1827 421 Fragments arranged by size: 1827 929 421 PmlI CAC'GTG Cuts at: 0 504 1224 1674 3177 Size: 504 720 450 1503 Fragments arranged by size: 1503 720 504 450 PshAI GACnn'nnGTC Cuts at: 0 2524 2590 3177 Size: 2524 66 587 Fragments arranged by size: 2524 587 66 PspOMI G'GGCC_C Cuts at: 0 952 2009 2277 3030 3177 Size: 952 1057 268 753 147 Fragments arranged by size: 1057 952 753 268 147 PspXI vC'TCGA_Gb Cuts at: 0 2068 3177 Size: 2068 1109 PstI C_TGCA'G Cuts at: 0 323 770 1913 2408 2996 3177 Size: 323 447 1143 495 588 181 Fragments arranged by size: 1143 588 495 447 323 181 PvuII CAG'CTG Cuts at: 0 1169 1191 2166 2689 3162 3177 Size: 1169 22 975 523 473 15 Fragments arranged by size: 1169 975 523 473 22 15 SacI G_AGCT'C Cuts at: 0 845 3177 Size: 845 2332 SacII CC_GC'GG Cuts at: 0 710 1238 2284 3038 3177 Size: 710 528 1046 754 139 Fragments arranged by size: 1046 754 710 528 139 SapI GCTCTTCn'nnn_ Cuts at: 0 541 3177 Size: 541 2636 SfcI C'TryA_G Cuts at: 0 319 766 1909 2404 2992 3177 Size: 319 447 1143 495 588 185 Fragments arranged by size: 1143 588 495 447 319 185 SfiI GGCCn_nnn'nGGCC Cuts at: 0 374 1544 3177 Size: 374 1170 1633 Fragments arranged by size: 1633 1170 374 SfoI GGC'GCC Cuts at: 0 38 493 681 965 995 1246 1301 3177 Size: 38 455 188 284 30 251 55 1876 Fragments arranged by size: 1876 455 284 251 188 55 38 30 SgrAI Cr'CCGG_yG Cuts at: 0 511 3177 Size: 511 2666 SmaI CCC'GGG Cuts at: 0 695 730 951 2718 3048 3177 Size: 695 35 221 1767 330 129 Fragments arranged by size: 1767 695 330 221 129 35 SmlI C'TyrA_G Cuts at: 0 414 2068 3177 Size: 414 1654 1109 Fragments arranged by size: 1654 1109 414 StuI AGG'CCT Cuts at: 0 298 466 1468 1636 3177 Size: 298 168 1002 168 1541 Fragments arranged by size: 1541 1002 298 168 168 StyI C'CwwG_G Cuts at: 0 209 293 461 998 3177 Size: 209 84 168 537 2179 Fragments arranged by size: 2179 537 209 168 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 655 1825 2038 2673 3177 Size: 655 1170 213 635 504 Fragments arranged by size: 1170 655 635 504 213 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 109 873 1678 2748 2952 3177 Size: 109 764 805 1070 204 225 Fragments arranged by size: 1070 805 764 225 204 109 XhoI C'TCGA_G Cuts at: 0 2068 3177 Size: 2068 1109 XmaI C'CCGG_G Cuts at: 0 693 728 949 2716 3046 3177 Size: 693 35 221 1767 330 131 Fragments arranged by size: 1767 693 330 221 131 35 XmnI GAAnn'nnTTC Cuts at: 0 550 2867 3177 Size: 550 2317 310 Fragments arranged by size: 2317 550 310 Enzymes that do cut: AarI AfeI AflII AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FseI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PmlI PshAI PspOMI PspXI PstI PvuII SacI SacII SapI SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI TaqII XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII BmtI BplI BpuEI BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BssSI BstBI BstZ17I ClaI DraI DrdI EcoNI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PpuMI PsiI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SgfI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI ZraI