################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI Alw44I AlwI ApaLI ApoI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbrPI BciVI BclI BfaI BfrI BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MroI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfaNI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 1795 GACGTC GACGT^C AccI 979 GTCTAC GT^[AC][GT]AC AflIII 1379 ACGTGT A^C[GA][CT]GT AhdI 346 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 280 CCTGAGG CC^T[ACGT]AGG AscI 1036 GGCGCGCC GG^CGCGCC AspEI 346 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1138 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 1144 GTCTTC GAAGAC(2/6) BbvCI 164 CCTCAGC CCTCAGC(-5/-2) BcgI 1576 CGATACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1373 AGATCT A^GATCT BpuAI 1144 GTCTTC GAAGAC(2/6) BsaAI 1378 TACGTG [CT]AC^GT[GA] BsmAI 107 GTCTC GTCTC(1/5) BspHI 773 TCATGA T^CATGA BstEII 1234 GGTCACC G^GT[ACGT]ACC BstF5I 1812 CATCC GGATG(2/0) BstPI 1234 GGTCACC G^GT[ACGT]ACC BstYI 1373 AGATCT [GA]^GATC[CT] Bsu36I 280 CCTGAGG CC^T[ACGT]AGG DraIII 7 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 970 GACGCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 346 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 280 CCTGAGG CC^T[ACGT]AGG EcoNI 1191 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1234 GGTCACC G^GT[ACGT]ACC EcoT14I 1790 CCTTGG C^C[AT][AT]GG FokI 1812 CATCC GGATG(9/13) HinfI 142 GAGTC G^A[ACGT]TC HphI 1236 TCACC GGTGA(8/7) MaeIII 1235 GTCAC ^GT[ACGT]AC MflI 1373 AGATCT [GA]^GATC[CT] PflFI 1138 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 142 GAGTC GAGTC(4/5) RcaI 773 TCATGA T^CATGA SacI 268 GAGCTC GAGCT^C SapI 301 GAAGAGC GCTCTTC(1/4) SgrAI 1040 CGCCGGCG C[GA]^CCGG[CT]G StyI 1790 CCTTGG C^C[AT][AT]GG Tsp45I 1235 GTCAC ^GT[GC]AC Tth111I 1138 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 668 CCACCGACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1373 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 385 GTAC GT^AC 408 GTAC AlwNI 35 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG 736 CAGCGGCTG Aor51HI 1277 AGCGCT AGC^GCT 1469 AGCGCT ApaI 1110 GGGCCC GGGCC^C 1304 GGGCCC BalI 698 TGGCCA TGG^CCA 1589 TGGCCA BlpI 690 GCTGAGC GC^T[ACGT]AGC 1266 GCTCAGC Bpu1102I 690 GCTGAGC GC^T[ACGT]AGC 1266 GCTCAGC BsgI 526 GTGCAG GTGCAG(16/14) 1452 GTGCAG BspMI 845 ACCTGC ACCTGC(4/8) 1360 ACCTGC CelII 690 GCTGAGC GC^T[ACGT]AGC 1266 GCTCAGC DpnII 1374 GATC ^GATC 1516 GATC Eco47III 1277 AGCGCT AGC^GCT 1469 AGCGCT MboI 1374 GATC ^GATC 1516 GATC MluNI 698 TGGCCA TGG^CCA 1589 TGGCCA MscI 698 TGGCCA TGG^CCA 1589 TGGCCA NdeII 1374 GATC ^GATC 1516 GATC RsaI 385 GTAC GT^AC 408 GTAC Sau3AI 1374 GATC ^GATC 1516 GATC SexAI 422 ACCTGGT A^CC[AT]GGT 1139 ACCAGGT SfiI 1111 GGCCCCGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1636 GGCCCCACGGGCC SmlI 340 CTTGAG C^T[CT][GA]AG 1411 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI Ksp632I KspI MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV PflMI PpuMI PstI PvuII SacII Sau96I ScrFI SfcI SfoI SmaI TaqI TseI TspRI TthHB8I Van91I XmaCI XmaI