(Circular) (Six-base) MAPSORT of: pFN21AA0711.seq Check: 1012 from: 1 to: 6574 pFN21AA0711 6574 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3374 3374 Size: 6574 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3804 4384 278 Size: 53 83 186 936 2268 580 2468 Fragments arranged by size: 2468 2268 936 580 186 83 53 AccI GT'mk_AC Cuts at: 1937 2985 3917 1937 Size: 1048 932 4594 Fragments arranged by size: 4594 1048 932 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2116 4637 6021 6289 1632 Size: 484 2521 1384 268 1917 Fragments arranged by size: 2521 1917 1384 484 268 AfeI AGC'GCT Cuts at: 3284 3476 3284 Size: 192 6382 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 3384 5473 6450 1203 Size: 2181 2089 977 1327 Fragments arranged by size: 2181 2089 1327 977 AgeI A'CCGG_T Cuts at: 5405 5405 Size: 6574 AhdI GACnn_n'nnGTC Cuts at: 2356 5303 2356 Size: 2947 3627 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6574 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6574 AlwNI CAG_nnn'CTG Cuts at: 2045 2746 4317 5889 2045 Size: 701 1571 1572 2730 Fragments arranged by size: 2730 1572 1571 701 ApaI G_GGCC'C Cuts at: 3119 3313 3119 Size: 194 6380 ApaLI G'TGCA_C Cuts at: 4631 5787 4631 Size: 1156 5418 ApoI r'AATT_y Cuts at: 1493 3883 4109 4261 6370 1493 Size: 2390 226 152 2109 1697 Fragments arranged by size: 2390 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3042 3042 Size: 6574 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2363 2372 2395 2906 2950 3035 Size: 73 108 312 9 23 511 44 85 Cuts at: 3035 3330 3732 3852 3899 1870 Size: 295 402 120 47 4545 Fragments arranged by size: 4545 511 402 312 295 120 108 85 73 47 44 23 9 BamHI G'GATC_C Cuts at: 3904 4271 3904 Size: 367 6207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 2255 2317 2448 2657 Size: 325 217 627 294 174 62 131 209 Cuts at: 2657 3106 3319 3335 3683 3710 3788 3866 3895 Size: 449 213 16 348 27 78 78 29 Cuts at: 3895 5350 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 449 348 325 294 217 213 209 174 131 78 78 62 29 27 16 BanII G_rGCy'C Cuts at: 1391 2052 2277 2313 2373 2382 2814 2948 3119 Size: 661 225 36 60 9 432 134 171 Cuts at: 3119 3313 3398 3611 3893 1391 Size: 194 85 213 282 4072 Fragments arranged by size: 4072 661 432 282 225 213 194 171 134 85 60 36 9 BbeI G_GCGC'C Cuts at: 2085 2259 2321 2452 2661 3110 3323 3339 3687 Size: 174 62 131 209 449 213 16 348 Cuts at: 3687 3792 3870 2085 Size: 105 78 4789 Fragments arranged by size: 4789 449 348 213 209 174 131 105 78 62 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3142 928 Size: 971 1243 4360 Fragments arranged by size: 4360 1243 971 BbvCI CC'TCA_GC Cuts at: 2170 2170 Size: 6574 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3570 3604 4765 4799 1903 Size: 34 1633 34 1161 34 3678 Fragments arranged by size: 3678 1633 1161 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4466 5676 4466 Size: 1210 5364 BclI T'GATC_A Cuts at: 1548 1548 Size: 6574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2174 2835 3123 Size: 107 122 71 631 605 502 661 288 Cuts at: 3123 3317 3636 3648 3708 3768 5185 136 Size: 194 319 12 60 60 1417 1525 Fragments arranged by size: 1525 1417 661 631 605 502 319 288 194 122 107 71 60 60 12 BglII A'GATC_T Cuts at: 3378 6569 3378 Size: 3191 3383 BlpI GC'TnA_GC Cuts at: 2696 3272 3991 2696 Size: 576 719 5279 Fragments arranged by size: 5279 719 576 Bme1580I G_kGCm'C Cuts at: 1790 2303 3119 3313 3455 3713 4635 5791 1790 Size: 513 816 194 142 258 922 1156 2573 Fragments arranged by size: 2573 1156 922 816 513 258 194 142 BmgBI CAC'GTC Cuts at: 1318 2521 1318 Size: 1203 5371 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4305 5263 442 Size: 416 53 83 399 24 2888 958 1753 Fragments arranged by size: 2888 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2188 2456 2816 3199 5234 1239 Size: 949 268 360 383 2035 2579 Fragments arranged by size: 2579 2035 949 383 360 268 Bpu10I CC'TnA_GC Cuts at: 2170 2723 2170 Size: 553 6021 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2366 3401 4699 5564 5862 2366 Size: 1035 1298 865 298 3078 Fragments arranged by size: 3078 1298 1035 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5237 882 Size: 617 3738 2219 Fragments arranged by size: 3738 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3385 4365 493 Size: 711 2181 980 2702 Fragments arranged by size: 2702 2181 980 711 BsaBI GATnn'nnATC Cuts at: 6568 6568 Size: 6574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 2256 2318 2449 Size: 53 83 186 936 549 174 62 131 Cuts at: 2449 2574 2658 2679 2763 3107 3320 3336 3684 Size: 125 84 21 84 344 213 16 348 Cuts at: 3684 3789 3801 3867 4381 4763 5398 275 Size: 105 12 66 514 382 635 1451 Fragments arranged by size: 1451 936 635 549 514 382 348 344 213 186 174 131 125 105 84 84 83 66 62 53 21 16 12 BsaWI w'CCGG_w Cuts at: 5007 5405 5679 5826 6237 5007 Size: 398 274 147 411 5344 Fragments arranged by size: 5344 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2219 2249 2264 2282 2294 Size: 30 165 30 816 30 15 18 12 Cuts at: 2294 2312 3440 3470 1178 Size: 18 1128 30 4282 Fragments arranged by size: 4282 1128 816 165 30 30 30 30 18 18 15 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2303 2381 2555 1186 Size: 1099 18 78 174 5205 Fragments arranged by size: 5205 1099 174 78 18 BseYI C'CCAG_C Cuts at: 3306 3784 5777 6273 3306 Size: 478 1993 496 3607 Fragments arranged by size: 3607 1993 496 478 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2552 3478 1163 Size: 573 816 926 4259 Fragments arranged by size: 4259 926 816 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2827 2881 2982 3006 3127 3304 Size: 275 186 826 54 101 24 121 177 Cuts at: 3304 3483 4024 4785 5813 1540 Size: 179 541 761 1028 2301 Fragments arranged by size: 2301 1028 826 761 541 275 186 179 177 121 101 54 24 BsiHKAI G_wGCw'C Cuts at: 2013 2277 3816 4635 4720 5791 2013 Size: 264 1539 819 85 1071 2796 Fragments arranged by size: 2796 1539 1071 819 264 85 BsmI GAATG_Cn' Cuts at: 4085 4178 4085 Size: 93 6481 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2052 2277 2303 2313 2373 2382 Size: 399 223 39 225 26 10 60 9 Cuts at: 2382 2814 2948 3119 3313 3398 3455 3611 3713 Size: 432 134 171 194 85 57 156 102 Cuts at: 3713 3816 3893 4635 4720 5791 1391 Size: 103 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 432 399 225 223 194 171 156 134 103 102 85 85 77 60 57 39 26 10 9 BspEI T'CCGG_A Cuts at: 6237 6237 Size: 6574 BspHI T'CATG_A Cuts at: 2778 4463 6193 2778 Size: 1685 1730 3159 Fragments arranged by size: 3159 1730 1685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2859 3374 3929 844 Size: 2015 515 555 3489 Fragments arranged by size: 3489 2015 555 515 BsrBI CCG'CTC Cuts at: 1394 1823 2230 2845 3528 3564 4461 1394 Size: 429 407 615 683 36 897 3507 Fragments arranged by size: 3507 897 683 615 429 407 36 BsrDI GCAATG_nn' Cuts at: 65 1248 5069 5243 65 Size: 1183 3821 174 1396 Fragments arranged by size: 3821 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2243 2451 2630 2876 2911 2934 3046 Size: 110 433 208 179 246 35 23 112 Cuts at: 3046 3746 5218 5405 1700 Size: 700 1472 187 2869 Fragments arranged by size: 2869 1472 700 433 246 208 187 179 112 110 35 23 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 2293 2458 2460 2483 2502 2504 2506 2634 Size: 368 165 2 23 19 2 2 128 Cuts at: 2634 2636 2668 2872 2923 2966 3042 3129 3131 Size: 2 32 204 51 43 76 87 2 Cuts at: 3131 3206 3295 1925 Size: 75 89 5204 Fragments arranged by size: 5204 368 204 165 128 89 87 76 75 51 43 32 23 19 2 2 2 2 2 BssSI C'ACGA_G Cuts at: 4634 5646 4634 Size: 1012 5562 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6574 BstBI TT'CG_AA Cuts at: 5378 5378 Size: 6574 BstEII G'GTnAC_C Cuts at: 1184 3239 1184 Size: 2055 4519 BstYI r'GATC_y Cuts at: 1972 3378 3904 4271 4656 4673 6125 6211 6223 Size: 1406 526 367 385 17 1452 86 12 Cuts at: 6223 6569 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1406 526 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2286 2286 Size: 6574 BtgI C'CryG_G Cuts at: 513 1065 2004 2216 2313 2682 2898 3111 3168 Size: 552 939 212 97 369 216 213 57 Cuts at: 3168 3252 3315 3345 3370 3428 3535 3645 513 Size: 84 63 30 25 58 107 110 3442 Fragments arranged by size: 3442 939 552 369 216 213 212 110 107 97 84 63 58 57 30 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5835 Fragments arranged by size: 5835 683 56 BtsI GCAGTG_nn' Cuts at: 2240 3586 3608 4090 4884 4904 2240 Size: 1346 22 482 794 20 3910 Fragments arranged by size: 3910 1346 794 482 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 3878 3878 Size: 6574 DraIII CAC_nnn'GTG Cuts at: 2017 2017 Size: 6574 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2981 5581 809 Size: 507 1665 2600 1802 Fragments arranged by size: 2600 1802 1665 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2616 2628 Size: 54 1425 50 275 18 357 429 12 Cuts at: 2628 2703 2730 2824 2878 2895 2979 3003 3124 Size: 75 27 94 54 17 84 24 121 Cuts at: 3124 3165 3480 3594 4021 4910 6539 8 Size: 41 315 114 427 889 1629 43 Fragments arranged by size: 1629 1425 889 429 427 357 315 275 121 114 94 84 75 54 54 50 43 41 27 24 18 17 12 EagI C'GGCC_G Cuts at: 1537 1812 2824 2878 2979 3003 3124 3480 4021 Size: 275 1012 54 101 24 121 356 541 Cuts at: 4021 1537 Size: 4090 Fragments arranged by size: 4090 1012 541 356 275 121 101 54 24 EarI CTCTTCn'nnn_ Cuts at: 1353 2139 2301 3092 3407 4504 1353 Size: 786 162 791 315 1097 3423 Fragments arranged by size: 3423 1097 791 786 315 162 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2192 5161 5535 5681 387 Size: 1064 138 603 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 603 374 146 138 EcoICRI GAG'CTC Cuts at: 2275 2275 Size: 6574 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2116 2188 2456 2816 3199 4637 5234 Size: 393 484 72 268 360 383 1438 597 Cuts at: 5234 6021 6289 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1438 787 597 484 393 383 360 268 268 72 EcoNI CCTnn'n_nnAGG Cuts at: 3200 3200 Size: 6574 EcoO109I rG'GnC_Cy Cuts at: 2350 2719 2848 3116 3192 3310 2350 Size: 369 129 268 76 118 5614 Fragments arranged by size: 5614 369 268 129 118 76 EcoRI G'AATT_C Cuts at: 3883 4261 3883 Size: 378 6196 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 2085 2259 2321 2452 2555 2661 2906 3110 Size: 407 174 62 131 103 106 245 204 Cuts at: 3110 3186 3286 3323 3339 3478 3687 3721 3785 Size: 76 100 37 16 139 209 34 64 Cuts at: 3785 3792 3870 5721 1678 Size: 7 78 1851 2531 Fragments arranged by size: 2531 1851 407 245 209 204 174 139 131 106 103 100 78 76 64 62 37 34 16 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 2342 2374 5215 Size: 32 135 32 692 32 46 32 2841 Cuts at: 5215 5247 5289 5321 1373 Size: 32 42 32 2626 Fragments arranged by size: 2841 2626 692 135 46 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3918 4164 669 Size: 988 281 1980 246 3079 Fragments arranged by size: 3079 1980 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KasI G'GCGC_C Cuts at: 2081 2255 2317 2448 2657 3106 3319 3335 3683 Size: 174 62 131 209 449 213 16 348 Cuts at: 3683 3788 3866 2081 Size: 105 78 4789 Fragments arranged by size: 4789 449 348 213 209 174 131 105 78 62 16 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3165 5688 5872 1305 Size: 1860 2523 184 2007 Fragments arranged by size: 2523 2007 1860 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2705 3596 10 Size: 54 1425 343 873 891 2988 Fragments arranged by size: 2988 1425 891 873 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4532 4891 5050 518 Size: 648 3366 359 159 2042 Fragments arranged by size: 3366 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2174 2208 2218 2315 2583 2650 2695 2743 Size: 568 34 10 97 268 67 45 48 Cuts at: 2743 2900 3113 3177 3217 3306 3325 3430 3537 Size: 157 213 64 40 89 19 105 107 Cuts at: 3537 3666 3990 4667 5815 6060 1606 Size: 129 324 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 568 324 268 245 213 157 129 107 105 97 89 67 64 48 45 40 34 19 10 NaeI GCC'GGC Cuts at: 2245 2453 2632 2878 2913 2936 3048 3748 2245 Size: 208 179 246 35 23 112 700 5071 Fragments arranged by size: 5071 700 246 208 179 112 35 23 NarI GG'CG_CC Cuts at: 2082 2256 2318 2449 2658 3107 3320 3336 3684 Size: 174 62 131 209 449 213 16 348 Cuts at: 3684 3789 3867 2082 Size: 105 78 4789 Fragments arranged by size: 4789 449 348 213 209 174 131 105 78 62 16 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5083 Fragments arranged by size: 5083 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NgoMIV G'CCGG_C Cuts at: 2243 2451 2630 2876 2911 2934 3046 3746 2243 Size: 208 179 246 35 23 112 700 5071 Fragments arranged by size: 5071 700 246 208 179 112 35 23 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 3932 5477 1132 Size: 97 2703 1545 2229 Fragments arranged by size: 2703 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2039 1348 Size: 691 5883 PciI A'CATG_T Cuts at: 5473 5473 Size: 6574 PflMI CCAn_nnn'nTGG Cuts at: 1108 2794 2893 3592 3603 1108 Size: 1686 99 699 11 4079 Fragments arranged by size: 4079 1686 699 99 11 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4643 4675 6156 6188 4643 Size: 32 1481 32 5029 Fragments arranged by size: 5029 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2350 2719 2848 2350 Size: 369 129 6076 Fragments arranged by size: 6076 369 129 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PspOMI G'GGCC_C Cuts at: 3115 3309 3115 Size: 194 6380 PstI C_TGCA'G Cuts at: 830 2111 2649 3657 3926 830 Size: 1281 538 1008 269 3478 Fragments arranged by size: 3478 1281 1008 538 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 2650 2695 3177 3217 2650 Size: 45 482 40 6007 Fragments arranged by size: 6007 482 45 40 SacI G_AGCT'C Cuts at: 2277 2277 Size: 6574 SacII CC_GC'GG Cuts at: 2219 2316 2901 3114 3431 3538 2219 Size: 97 585 213 317 107 5255 Fragments arranged by size: 5255 585 317 213 107 97 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SapI GCTCTTCn'nnn_ Cuts at: 2301 2301 Size: 6574 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 4822 6534 4822 Size: 1712 4862 SexAI A'CCwGG_T Cuts at: 2427 3144 2427 Size: 717 5857 SfcI C'TryA_G Cuts at: 826 1045 2107 2578 2645 3653 3922 5057 5738 Size: 219 1062 471 67 1008 269 1135 681 Cuts at: 5738 5929 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1062 1008 681 471 269 219 191 67 SfiI GGCCn_nnn'nGGCC Cuts at: 3123 3648 3123 Size: 525 6049 SfoI GGC'GCC Cuts at: 2083 2257 2319 2450 2659 3108 3321 3337 3685 Size: 174 62 131 209 449 213 16 348 Cuts at: 3685 3790 3868 2083 Size: 105 78 4789 Fragments arranged by size: 4789 449 348 213 209 174 131 105 78 62 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 3046 1700 Size: 1346 5228 SmaI CCC'GGG Cuts at: 1872 2053 2374 2397 3332 3734 3901 1872 Size: 181 321 23 935 402 167 4545 Fragments arranged by size: 4545 935 402 321 181 167 23 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 3416 4678 5579 5841 820 Size: 197 926 402 1071 1262 901 262 1553 Fragments arranged by size: 1553 1262 1071 926 901 402 262 197 SnaBI TAC'GTA Cuts at: 493 4365 493 Size: 3872 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 3932 1132 Size: 2800 3774 SspI AAT'ATT Cuts at: 5 52 4498 5 Size: 47 4446 2081 Fragments arranged by size: 4446 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6574 StyI C'CwwG_G Cuts at: 513 1065 2004 3795 4013 513 Size: 552 939 1791 218 3074 Fragments arranged by size: 3074 1791 939 552 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4601 4618 4771 4956 5401 325 Size: 1255 3021 17 153 185 445 1498 Fragments arranged by size: 3021 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4820 6532 96 Size: 274 80 33 51 1443 2843 1712 138 Fragments arranged by size: 2843 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2219 2251 2282 2314 3440 3472 6534 6566 2219 Size: 32 31 32 1126 32 3062 32 2227 Fragments arranged by size: 3062 2227 1126 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 1316 3146 1316 Size: 1830 4744 XbaI T'CTAG_A Cuts at: 3910 3910 Size: 6574 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2680 2680 Size: 6574 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 2051 2372 2395 3330 3732 3899 1870 Size: 181 321 23 935 402 167 4545 Fragments arranged by size: 4545 935 402 321 181 167 23 XmnI GAAnn'nnTTC Cuts at: 4703 4703 Size: 6574 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3802 4382 276 Size: 53 83 186 936 2268 580 2468 Fragments arranged by size: 2468 2268 936 580 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SrfI SwaI