(Circular) (Six-base) MAPSORT of: pFN21AE5789.seq Check: 9398 from: 1 to: 6553 pFN21AE5789 6553 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3449 3449 Size: 6553 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4363 278 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2148 3217 3616 3631 3646 3896 1937 Size: 211 1069 399 15 15 250 4594 Fragments arranged by size: 4594 1069 399 250 211 15 15 Acc65I G'GTAC_C Cuts at: 3874 3874 Size: 6553 AclI AA'CG_TT Cuts at: 1563 4680 5053 1563 Size: 3117 373 3063 Fragments arranged by size: 3117 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4616 6000 6268 1632 Size: 2984 1384 268 1917 Fragments arranged by size: 2984 1917 1384 268 AfeI AGC'GCT Cuts at: 2262 2262 Size: 6553 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6356 AflIII A'CryG_T Cuts at: 1203 2484 2590 3293 3295 5452 6429 1203 Size: 1281 106 703 2 2157 977 1327 Fragments arranged by size: 2157 1327 1281 977 703 106 2 AgeI A'CCGG_T Cuts at: 5384 5384 Size: 6553 AhdI GACnn_n'nnGTC Cuts at: 5282 5282 Size: 6553 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6553 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6553 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2667 2699 2667 Size: 32 6521 AlwNI CAG_nnn'CTG Cuts at: 3041 4296 5868 3041 Size: 1255 1572 3726 Fragments arranged by size: 3726 1572 1255 ApaI G_GGCC'C Cuts at: 3392 3392 Size: 6553 ApaLI G'TGCA_C Cuts at: 4610 5766 4610 Size: 1156 5397 ApoI r'AATT_y Cuts at: 1493 2571 2787 3426 3862 4088 4240 6349 1493 Size: 1078 216 639 436 226 152 2109 1697 Fragments arranged by size: 2109 1697 1078 639 436 226 216 152 AseI AT'TA_AT Cuts at: 160 1032 3447 5107 160 Size: 872 2415 1660 1606 Fragments arranged by size: 2415 1660 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 AvaI C'yCGr_G Cuts at: 1870 1943 2982 3249 3878 1870 Size: 73 1039 267 629 4545 Fragments arranged by size: 4545 1039 629 267 73 BamHI G'GATC_C Cuts at: 2353 2551 3883 4250 2353 Size: 198 1332 367 4656 Fragments arranged by size: 4656 1332 367 198 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3874 5329 618 Size: 325 217 627 2087 1455 1842 Fragments arranged by size: 2087 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2667 2941 3057 3189 3392 3872 1391 Size: 1276 274 116 132 203 480 4072 Fragments arranged by size: 4072 1276 480 274 203 132 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2743 928 Size: 971 844 4738 Fragments arranged by size: 4738 971 844 BbvCI CC'TCA_GC Cuts at: 2715 2721 2715 Size: 6 6547 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4744 4778 1903 Size: 34 2807 34 3678 Fragments arranged by size: 3678 2807 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 4445 5655 3818 Size: 627 1210 4716 Fragments arranged by size: 4716 1210 627 BclI T'GATC_A Cuts at: 1548 3741 1548 Size: 2193 4360 BfrBI ATG'CAT Cuts at: 3097 3097 Size: 6553 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5164 136 Size: 107 122 71 631 605 3492 1525 Fragments arranged by size: 3492 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6548 6548 Size: 6553 BlpI GC'TnA_GC Cuts at: 3970 3970 Size: 6553 Bme1580I G_kGCm'C Cuts at: 1790 3392 4614 5770 1790 Size: 1602 1222 1156 2573 Fragments arranged by size: 2573 1602 1222 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6553 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2888 3393 4284 Size: 416 53 83 399 24 1471 505 891 Cuts at: 4284 5242 442 Size: 958 1753 Fragments arranged by size: 1753 1471 958 891 505 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6553 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2691 2781 2871 3063 3537 3864 5213 1239 Size: 1452 90 90 192 474 327 1349 2579 Fragments arranged by size: 2579 1452 1349 474 327 192 90 90 Bpu10I CC'TnA_GC Cuts at: 2715 2721 3792 2715 Size: 6 1071 5476 Fragments arranged by size: 5476 1071 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4678 5543 5841 4678 Size: 865 298 5390 Fragments arranged by size: 5390 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2019 2201 3314 5216 882 Size: 617 520 182 1113 1902 2219 Fragments arranged by size: 2219 1902 1113 617 520 182 BsaAI yAC'GTr Cuts at: 493 1204 3017 3296 4344 493 Size: 711 1813 279 1048 2702 Fragments arranged by size: 2702 1813 1048 711 279 BsaBI GATnn'nnATC Cuts at: 2543 6547 2543 Size: 4004 2549 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4360 4742 5377 275 Size: 53 83 186 936 2827 382 635 1451 Fragments arranged by size: 2827 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4986 5384 5658 5805 6216 4986 Size: 398 274 147 411 5323 Fragments arranged by size: 5323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2438 3837 3867 1178 Size: 30 165 30 1005 30 1399 30 3864 Fragments arranged by size: 3864 1399 1005 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2315 2703 2748 2868 2970 3322 3459 Size: 919 210 388 45 120 102 352 137 Cuts at: 3459 3594 3639 3654 1186 Size: 135 45 15 4085 Fragments arranged by size: 4085 919 388 352 210 137 135 120 102 45 45 15 BseYI C'CCAG_C Cuts at: 5756 6252 5756 Size: 496 6057 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2939 1163 Size: 573 1203 4777 Fragments arranged by size: 4777 1203 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4003 4764 5792 1540 Size: 275 186 2002 761 1028 2301 Fragments arranged by size: 2301 2002 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2941 4614 4699 5770 2941 Size: 1673 85 1071 3724 Fragments arranged by size: 3724 1673 1071 85 BsmI GAATG_Cn' Cuts at: 2883 4064 4157 2883 Size: 1181 93 5279 Fragments arranged by size: 5279 1181 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2153 2153 Size: 6553 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2667 2941 3057 3189 3392 3872 4614 Size: 399 877 274 116 132 203 480 742 Cuts at: 4614 4699 5770 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 877 742 480 399 274 203 132 116 85 BspEI T'CCGG_A Cuts at: 6216 6216 Size: 6553 BspHI T'CATG_A Cuts at: 3112 4442 6172 3112 Size: 1330 1730 3493 Fragments arranged by size: 3493 1730 1330 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3449 3739 3908 844 Size: 2605 290 169 3489 Fragments arranged by size: 3489 2605 290 169 BsrBI CCG'CTC Cuts at: 1394 1823 2908 4440 1394 Size: 429 1085 1532 3507 Fragments arranged by size: 3507 1532 1085 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2040 2865 5048 5222 65 Size: 1183 792 825 2183 174 1396 Fragments arranged by size: 2183 1396 1183 825 792 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5197 5384 1700 Size: 110 3387 187 2869 Fragments arranged by size: 3387 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6553 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6553 BssSI C'ACGA_G Cuts at: 3120 3264 4613 5625 3120 Size: 144 1349 1012 4048 Fragments arranged by size: 4048 1349 1012 144 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6553 BstBI TT'CG_AA Cuts at: 5357 5357 Size: 6553 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6553 BstXI CCAn_nnnn'nTGG Cuts at: 3346 3346 Size: 6553 BstYI r'GATC_y Cuts at: 1972 2353 2551 2929 3163 3883 4250 4635 4652 Size: 381 198 378 234 720 367 385 17 Cuts at: 4652 6104 6190 6202 6548 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 720 385 381 378 367 346 234 198 86 17 12 BstZ17I GTA'TAC Cuts at: 2149 3617 3632 3647 2149 Size: 1468 15 15 5055 Fragments arranged by size: 5055 1468 15 15 Bsu36I CC'TnA_GG Cuts at: 3471 3471 Size: 6553 BtgI C'CryG_G Cuts at: 513 1065 3270 513 Size: 552 2205 3796 Fragments arranged by size: 3796 2205 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3269 3344 487 Size: 683 56 2043 75 3696 Fragments arranged by size: 3696 2043 683 75 56 BtsI GCAGTG_nn' Cuts at: 2045 4069 4863 4883 2045 Size: 2024 794 20 3715 Fragments arranged by size: 3715 2024 794 20 ClaI AT'CG_AT Cuts at: 1554 2041 2361 2384 1554 Size: 487 320 23 5723 Fragments arranged by size: 5723 487 320 23 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3649 3684 529 Size: 35 3085 35 3398 Fragments arranged by size: 3398 3085 35 35 DraI TTT'AAA Cuts at: 3819 3857 3819 Size: 38 6515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5560 809 Size: 507 4244 1802 Fragments arranged by size: 4244 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4000 4889 6518 Size: 54 1425 50 275 18 2170 889 1629 Cuts at: 6518 8 Size: 43 Fragments arranged by size: 2170 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4000 1537 Size: 275 2188 4090 Fragments arranged by size: 4090 2188 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4483 1353 Size: 3130 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5140 5514 5660 387 Size: 1064 138 3551 374 146 1280 Fragments arranged by size: 3551 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2939 2939 Size: 6553 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2691 2781 2871 3063 3537 3864 4616 Size: 393 1059 90 90 192 474 327 752 Cuts at: 4616 5213 6000 6268 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1059 787 752 597 474 393 327 268 192 90 90 EcoNI CCTnn'n_nnAGG Cuts at: 2982 2982 Size: 6553 EcoO109I rG'GnC_Cy Cuts at: 2675 3086 3194 3388 3389 3410 3434 3476 3487 Size: 411 108 194 1 21 24 42 11 Cuts at: 3487 2675 Size: 5741 Fragments arranged by size: 5741 411 194 108 42 24 21 11 1 EcoRI G'AATT_C Cuts at: 2787 3426 3862 4240 2787 Size: 639 436 378 5100 Fragments arranged by size: 5100 639 436 378 FspI TGC'GCA Cuts at: 5059 5059 Size: 6553 HaeII r_GCGC'y Cuts at: 1678 2264 2330 5700 1678 Size: 586 66 3370 2531 Fragments arranged by size: 3370 2531 586 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2755 2787 2914 Size: 32 135 32 524 32 627 32 127 Cuts at: 2914 2946 3247 3279 3754 3786 5194 5226 5268 Size: 32 301 32 475 32 1408 32 42 Cuts at: 5268 5300 1373 Size: 32 2626 Fragments arranged by size: 2626 1408 627 524 475 301 135 127 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3897 4143 669 Size: 988 281 1959 246 3079 Fragments arranged by size: 3079 1959 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6553 HpaI GTT'AAC Cuts at: 4143 4143 Size: 6553 KpnI G_GTAC'C Cuts at: 3878 3878 Size: 6553 MfeI C'AATT_G Cuts at: 4152 4152 Size: 6553 MluI A'CGCG_T Cuts at: 6429 6429 Size: 6553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5667 5851 1305 Size: 4362 184 2007 Fragments arranged by size: 4362 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4731 Fragments arranged by size: 4731 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4511 4870 5029 518 Size: 648 3345 359 159 2042 Fragments arranged by size: 3345 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2813 3969 4646 5794 6039 1606 Size: 1207 1156 677 1148 245 2120 Fragments arranged by size: 2120 1207 1156 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3270 513 Size: 552 2205 3796 Fragments arranged by size: 3796 2205 552 NdeI CA'TA_TG Cuts at: 387 2077 387 Size: 1690 4863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6553 NotI GC'GGCC_GC Cuts at: 4000 4000 Size: 6553 NsiI A_TGCA'T Cuts at: 3099 3099 Size: 6553 NspI r_CATG'y Cuts at: 1132 1229 2488 2594 3559 3911 5456 1132 Size: 97 1259 106 965 352 1545 2229 Fragments arranged by size: 2229 1545 1259 965 352 106 97 PasI CC'CwG_GG Cuts at: 1348 2660 1348 Size: 1312 5241 PciI A'CATG_T Cuts at: 2484 2590 5452 2484 Size: 106 2862 3585 Fragments arranged by size: 3585 2862 106 PflMI CCAn_nnn'nTGG Cuts at: 1108 2888 1108 Size: 1780 4773 PfoI T'CCnGG_A Cuts at: 2821 2848 3514 2821 Size: 27 666 5860 Fragments arranged by size: 5860 666 27 PmeI GTTT'AAAC Cuts at: 3857 3857 Size: 6553 PmlI CAC'GTG Cuts at: 3017 3296 3017 Size: 279 6274 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4622 4654 6135 6167 4622 Size: 32 1481 32 5008 Fragments arranged by size: 5008 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2675 3086 3434 3476 3487 2675 Size: 411 348 42 11 5741 Fragments arranged by size: 5741 411 348 42 11 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6553 PsiI TTA'TAA Cuts at: 4123 4123 Size: 6553 PspOMI G'GGCC_C Cuts at: 3388 3388 Size: 6553 PspXI vC'TCGA_Gb Cuts at: 2982 2982 Size: 6553 PstI C_TGCA'G Cuts at: 830 2326 2818 3769 3905 830 Size: 1496 492 951 136 3478 Fragments arranged by size: 3478 1496 951 492 136 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6553 PvuII CAG'CTG Cuts at: 2813 2813 Size: 6553 SacI G_AGCT'C Cuts at: 2941 2941 Size: 6553 SalI G'TCGA_C Cuts at: 1936 3895 1936 Size: 1959 4594 SanDI GG'GwC_CC Cuts at: 3086 3476 3086 Size: 390 6163 SbfI CC_TGCA'GG Cuts at: 3905 3905 Size: 6553 ScaI AGT'ACT Cuts at: 4801 6513 4801 Size: 1712 4841 SfcI C'TryA_G Cuts at: 826 1045 2322 2814 3765 3901 5036 5717 5908 Size: 219 1277 492 951 136 1135 681 191 Cuts at: 5908 826 Size: 1471 Fragments arranged by size: 1471 1277 1135 951 681 492 219 191 136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6553 SmaI CCC'GGG Cuts at: 1872 3880 1872 Size: 2008 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2982 3249 4657 5558 5820 820 Size: 197 926 1039 267 1408 901 262 1553 Fragments arranged by size: 1553 1408 1039 926 901 267 262 197 SnaBI TAC'GTA Cuts at: 493 4344 493 Size: 3851 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6553 SphI G_CATG'C Cuts at: 1132 3559 3911 1132 Size: 2427 352 3774 Fragments arranged by size: 3774 2427 352 SspI AAT'ATT Cuts at: 5 52 4477 5 Size: 47 4425 2081 Fragments arranged by size: 4425 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6553 StyI C'CwwG_G Cuts at: 513 1065 2172 2314 3270 3992 513 Size: 552 1107 142 956 722 3074 Fragments arranged by size: 3074 1107 956 722 552 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4580 4597 4750 4935 5380 325 Size: 1255 3000 17 153 185 445 1498 Fragments arranged by size: 3000 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4799 6511 96 Size: 274 80 33 51 1443 2822 1712 138 Fragments arranged by size: 2822 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2589 2621 6513 6545 2589 Size: 32 3892 32 2597 Fragments arranged by size: 3892 2597 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6553 XbaI T'CTAG_A Cuts at: 3889 3889 Size: 6553 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3064 3839 2011 Size: 1053 775 4725 Fragments arranged by size: 4725 1053 775 XhoI C'TCGA_G Cuts at: 1943 2982 3249 1943 Size: 1039 267 5247 Fragments arranged by size: 5247 1039 267 XmaI C'CCGG_G Cuts at: 1870 3878 1870 Size: 2008 4545 XmnI GAAnn'nnTTC Cuts at: 4682 4682 Size: 6553 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4361 276 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BplI BsiWI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI