################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AccII AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI BstXI BstYI Bsu36I CelII CfoI Cfr10I CpoI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FauI FseI FspI HaeII HhaI HinP1I HincII HindII HpaI KasI KspI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 196 GACGTC GACGT^C Acc65I 478 GGTACC G^GTACC AccI 573 GTATAC GT^[AC][GT]AC AcyI 196 GACGTC G[GA]^CG[CT]C AfaI 479 GTAC GT^AC AlwI 717 GGATC GGATC(4/5) AlwNI 1214 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 504 GGGCCC GGGCC^C Asp718I 478 GGTACC G^GTACC AvaI 1633 CCCGGG C^[CT]CG[GA]G BanI 478 GGTACC G^G[CT][GA]CC BanII 504 GGGCCC G[GA]GC[CT]^C BcgI 677 GCAATGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 657 GTATCC GTATCC(6/5) BclI 1601 TGATCA T^GATCA BsaHI 196 GACGTC G[GA]^CG[CT]C BseRI 1734 CTCCTC GAGGAG(10/8) BsgI 1643 GTGCAG GTGCAG(16/14) BsiEI 848 CGGTCG CG[GA][CT]^CG BsmAI 337 GAGAC GTCTC(1/5) BsmFI 199 GTCCC GGGAC(10/14) BsmI 760 GAATGC GAATGC(1/-1) BsoBI 1633 CCCGGG C^[CT]CG[GA]G BspDI 401 ATCGAT AT^CGAT BsrDI 677 GCAATG GCAATG(2/0) Bst1107I 573 GTATAC GTA^TAC BstZ17I 573 GTATAC GTA^TAC ClaI 401 ATCGAT AT^CGAT DraI 1005 TTTAAA TTT^AAA EcoT14I 566 CCAAGG C^C[AT][AT]GG EcoT22I 1079 ATGCAT ATGCA^T FbaI 1601 TGATCA T^GATCA HapII 1634 CCGG C^CGG Hin1I 196 GACGTC G[GA]^CG[CT]C HindIII 1678 AAGCTT A^AGCTT HpaII 1634 CCGG C^CGG KpnI 478 GGTACC GGTAC^C MaeIII 811 GTCAC ^GT[ACGT]AC MspI 1634 CCGG C^CGG NsiI 1079 ATGCAT ATGCA^T NspI 1081 GCATGT [GA]CATG^[CT] PleI 1137 GACTC GAGTC(4/5) PpuMI 1685 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 1331 CAGCTG CAG^CTG RsaI 479 GTAC GT^AC SapI 30 GCTCTTC GCTCTTC(1/4) SfcI 1479 CTACAG C^T[GA][CT]AG SmaI 1633 CCCGGG CCC^GGG StyI 566 CCAAGG C^C[AT][AT]GG Tsp45I 811 GTCAC ^GT[GC]AC XmaCI 1633 CCCGGG C^CCGGG XmaI 1633 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AhdI 248 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 493 GACACTTTGTC AspEI 248 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 493 GACACTTTGTC AspHI 461 GAGCAC G[AT]GC[AT]^C 1251 GTGCTC AvaII 1686 GGTCC G^G[AT]CC 1756 GGACC BcnI 1633 CCCGG CC^[GC]GG 1634 CCGGG BsaBI 1027 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1044 GATTGAAATC BsiHKAI 461 GAGCAC G[AT]GC[AT]^C 1251 GTGCTC BstAPI 463 GCACTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1102 GCAGCTATTGC BstF5I 1693 CATCC GGATG(2/0) 998 GGATG DraII 1617 AGGGCCT [GA]G^G[ACGT]CC[CT] 1685 GGGTCCT Eam1105I 248 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 493 GACACTTTGTC EarI 31 CTCTTC CTCTTC(1/4) 334 GAAGAG EcoO109I 1617 AGGGCCT [GA]G^G[ACGT]CC[CT] 1685 GGGTCCT EcoRV 538 GATATC GAT^ATC 1288 GATATC FokI 1693 CATCC GGATG(9/13) 998 GGATG HgaI 528 GCGTC GACGC(5/10) 1670 GCGTC Ksp632I 31 CTCTTC CTCTTC(1/4) 334 GAAGAG MamI 1027 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1044 GATTGAAATC MspA1I 1217 CCGCTG C[AC]G^C[GT]G 1331 CAGCTG NciI 1633 CCCGG CC^[GC]GG 1634 CCGGG PflMI 36 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 483 CCATGATTTGG Van91I 36 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 483 CCATGATTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AcsI AluI ApoI BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeI MaeII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SspI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I