(Circular) (Six-base) MAPSORT of: pFN21AE5766.seq Check: 8212 from: 1 to: 6472 pFN21AE5766 6472 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2205 4282 278 Size: 53 83 186 936 669 2077 2468 Fragments arranged by size: 2468 2077 936 669 186 83 53 AccI GT'mk_AC Cuts at: 1937 2579 3815 1937 Size: 642 1236 4594 Fragments arranged by size: 4594 1236 642 Acc65I G'GTAC_C Cuts at: 2483 3793 2483 Size: 1310 5162 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 2414 2523 2886 3255 3401 4535 5919 Size: 608 174 109 363 369 146 1134 1384 Cuts at: 5919 6187 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1134 608 369 363 268 174 146 109 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 5371 6348 1203 Size: 4168 977 1327 Fragments arranged by size: 4168 1327 977 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 2258 2503 5201 2258 Size: 245 2698 3529 Fragments arranged by size: 3529 2698 245 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6472 AlwNI CAG_nnn'CTG Cuts at: 3224 4215 5787 3224 Size: 991 1572 3909 Fragments arranged by size: 3909 1572 991 ApaI G_GGCC'C Cuts at: 2513 2513 Size: 6472 ApaLI G'TGCA_C Cuts at: 4529 5685 4529 Size: 1156 5316 ApoI r'AATT_y Cuts at: 1493 2072 2093 2549 3341 3716 3781 4007 4159 Size: 579 21 456 792 375 65 226 152 Cuts at: 4159 6268 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 792 579 456 375 226 152 65 21 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 3638 3797 1870 Size: 73 1695 159 4545 Fragments arranged by size: 4545 1695 159 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2645 2678 2645 Size: 33 6439 BamHI G'GATC_C Cuts at: 3802 4169 3802 Size: 367 6105 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2483 3793 5248 618 Size: 325 217 627 696 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 696 627 325 217 BanII G_rGCy'C Cuts at: 1391 2513 3791 1391 Size: 1122 1278 4072 Fragments arranged by size: 4072 1278 1122 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2254 3389 3594 928 Size: 971 355 1135 205 3806 Fragments arranged by size: 3806 1135 971 355 205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2671 2705 4663 4697 1903 Size: 34 50 34 650 34 1958 34 3678 Fragments arranged by size: 3678 1958 650 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2673 4364 5574 2673 Size: 1691 1210 3571 Fragments arranged by size: 3571 1691 1210 BclI T'GATC_A Cuts at: 1548 3606 1548 Size: 2058 4414 BfrBI ATG'CAT Cuts at: 3086 3086 Size: 6472 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5083 136 Size: 107 122 71 631 605 3411 1525 Fragments arranged by size: 3411 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6467 6467 Size: 6472 BlpI GC'TnA_GC Cuts at: 3889 3889 Size: 6472 Bme1580I G_kGCm'C Cuts at: 1790 2513 4533 5689 1790 Size: 723 2020 1156 2573 Fragments arranged by size: 2573 2020 1156 723 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6472 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4203 5161 442 Size: 416 53 83 399 24 2786 958 1753 Fragments arranged by size: 2786 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2268 2958 5132 1239 Size: 884 145 690 2174 2579 Fragments arranged by size: 2579 2174 884 690 145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4597 5462 5760 4597 Size: 865 298 5309 Fragments arranged by size: 5309 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5135 882 Size: 617 3636 2219 Fragments arranged by size: 3636 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4263 493 Size: 711 3059 2702 Fragments arranged by size: 3059 2702 711 BsaBI GATnn'nnATC Cuts at: 3036 3053 6466 3036 Size: 17 3413 3042 Fragments arranged by size: 3413 3042 17 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2202 4279 4661 5296 Size: 53 83 186 936 669 2077 382 635 Cuts at: 5296 275 Size: 1451 Fragments arranged by size: 2077 1451 936 669 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4905 5303 5577 5724 6135 4905 Size: 398 274 147 411 5242 Fragments arranged by size: 5242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6247 Fragments arranged by size: 6247 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3730 1186 Size: 2544 3928 BseYI C'CCAG_C Cuts at: 5675 6171 5675 Size: 496 5976 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3669 1163 Size: 573 1933 3966 Fragments arranged by size: 3966 1933 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2856 3922 4683 5711 1540 Size: 275 186 855 1066 761 1028 2301 Fragments arranged by size: 2301 1066 1028 855 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2470 3260 4533 4618 5689 2470 Size: 790 1273 85 1071 3253 Fragments arranged by size: 3253 1273 1071 790 85 BsmI GAATG_Cn' Cuts at: 2771 3983 4076 2771 Size: 1212 93 5167 Fragments arranged by size: 5167 1212 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2470 2513 3260 3791 4533 4618 5689 Size: 399 680 43 747 531 742 85 1071 Cuts at: 5689 1391 Size: 2174 Fragments arranged by size: 2174 1071 747 742 680 531 399 85 43 BspEI T'CCGG_A Cuts at: 6135 6135 Size: 6472 BspHI T'CATG_A Cuts at: 4361 6091 4361 Size: 1730 4742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3827 844 Size: 2983 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4359 1394 Size: 429 2536 3507 Fragments arranged by size: 3507 2536 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2689 4967 5141 65 Size: 1183 1441 2278 174 1396 Fragments arranged by size: 2278 1441 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5116 5303 1700 Size: 110 3306 187 2869 Fragments arranged by size: 3306 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6472 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 4532 5544 4532 Size: 1012 5460 BstAPI GCAn_nnn'nTGC Cuts at: 794 2474 3113 794 Size: 1680 639 4153 Fragments arranged by size: 4153 1680 639 BstBI TT'CG_AA Cuts at: 5276 5276 Size: 6472 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6472 BstYI r'GATC_y Cuts at: 1972 3802 4169 4554 4571 6023 6109 6121 6467 Size: 1830 367 385 17 1452 86 12 346 Cuts at: 6467 1972 Size: 1977 Fragments arranged by size: 1977 1830 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2580 2580 Size: 6472 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5920 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3658 487 Size: 683 56 2432 3301 Fragments arranged by size: 3301 2432 683 56 BtsI GCAGTG_nn' Cuts at: 3988 4782 4802 3988 Size: 794 20 5658 Fragments arranged by size: 5658 794 20 ClaI AT'CG_AT Cuts at: 1554 2407 1554 Size: 853 5619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6437 DraI TTT'AAA Cuts at: 3012 3776 3012 Size: 764 5708 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5479 809 Size: 507 4163 1802 Fragments arranged by size: 4163 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3919 4808 6437 Size: 54 1425 50 275 18 2089 889 1629 Cuts at: 6437 8 Size: 43 Fragments arranged by size: 2089 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3919 1537 Size: 275 2107 4090 Fragments arranged by size: 4090 2107 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2334 4402 1353 Size: 689 292 2068 3423 Fragments arranged by size: 3423 2068 689 292 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5059 5433 5579 387 Size: 1064 138 3470 374 146 1280 Fragments arranged by size: 3470 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2240 2268 2414 2523 2886 2958 Size: 393 491 117 28 146 109 363 72 Cuts at: 2958 3255 3401 4535 5132 5919 6187 1239 Size: 297 146 1134 597 787 268 1524 Fragments arranged by size: 1524 1134 787 597 491 393 363 297 268 146 146 117 109 72 28 EcoO109I rG'GnC_Cy Cuts at: 3623 3691 3623 Size: 68 6404 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 EcoRV GAT'ATC Cuts at: 2545 3295 2545 Size: 750 5722 FspI TGC'GCA Cuts at: 4978 4978 Size: 6472 HaeII r_GCGC'y Cuts at: 1678 5619 1678 Size: 3941 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2208 2240 2244 2266 2276 Size: 32 135 32 636 32 4 22 10 Cuts at: 2276 2298 2489 2521 2602 2634 3591 3623 5113 Size: 22 191 32 81 32 957 32 1490 Cuts at: 5113 5145 5187 5219 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1490 957 636 191 135 81 42 32 32 32 32 32 32 32 32 22 22 10 4 HincII GTy'rAC Cuts at: 669 1657 1938 3816 4062 669 Size: 988 281 1878 246 3079 Fragments arranged by size: 3079 1878 988 281 246 HindIII A'AGCT_T Cuts at: 748 3683 748 Size: 2935 3537 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KpnI G_GTAC'C Cuts at: 2487 3797 2487 Size: 1310 5162 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2938 3086 3229 3739 5586 5770 1305 Size: 1633 148 143 510 1847 184 2007 Fragments arranged by size: 2007 1847 1633 510 184 148 143 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4650 Fragments arranged by size: 4650 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3087 3137 3495 4430 4789 4948 518 Size: 648 1921 50 358 935 359 159 2042 Fragments arranged by size: 2042 1921 935 648 359 358 159 50 MspA1I CmG'CkG Cuts at: 1606 3224 3338 3888 4565 5713 5958 1606 Size: 1618 114 550 677 1148 245 2120 Fragments arranged by size: 2120 1618 1148 677 550 245 114 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5920 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NsiI A_TGCA'T Cuts at: 3088 3088 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 3090 3830 5375 1132 Size: 97 1861 740 1545 2229 Fragments arranged by size: 2229 1861 1545 740 97 PasI CC'CwG_GG Cuts at: 1348 2047 1348 Size: 699 5773 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 2494 1108 Size: 939 447 5086 Fragments arranged by size: 5086 939 447 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4541 4573 6054 6086 4541 Size: 32 1481 32 4927 Fragments arranged by size: 4927 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3691 3691 Size: 6472 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 4042 4042 Size: 6472 PspOMI G'GGCC_C Cuts at: 2509 2509 Size: 6472 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2361 2393 2767 2799 2361 Size: 32 374 32 6034 Fragments arranged by size: 6034 374 32 32 PstI C_TGCA'G Cuts at: 830 3824 830 Size: 2994 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 PvuII CAG'CTG Cuts at: 3338 3338 Size: 6472 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SapI GCTCTTCn'nnn_ Cuts at: 2042 2042 Size: 6472 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SfcI C'TryA_G Cuts at: 826 1045 3484 3820 4955 5636 5827 826 Size: 219 2439 336 1135 681 191 1471 Fragments arranged by size: 2439 1471 1135 681 336 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 3640 3799 1872 Size: 1768 159 4545 Fragments arranged by size: 4545 1768 159 SmlI C'TyrA_G Cuts at: 820 1017 1943 4576 5477 5739 820 Size: 197 926 2633 901 262 1553 Fragments arranged by size: 2633 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4263 493 Size: 3770 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SspI AAT'ATT Cuts at: 5 52 2452 2755 2834 4396 5 Size: 47 2400 303 79 1562 2081 Fragments arranged by size: 2400 2081 1562 303 79 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6472 StyI C'CwwG_G Cuts at: 513 1065 2571 3911 513 Size: 552 1506 1340 3074 Fragments arranged by size: 3074 1506 1340 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4499 4516 4669 4854 5299 325 Size: 1255 2919 17 153 185 445 1498 Fragments arranged by size: 2919 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4718 6430 96 Size: 274 80 33 51 1443 2741 1712 138 Fragments arranged by size: 2741 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6432 6464 6432 Size: 32 6440 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6472 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6472 XmaI C'CCGG_G Cuts at: 1870 3638 3797 1870 Size: 1768 159 4545 Fragments arranged by size: 4545 1768 159 XmnI GAAnn'nnTTC Cuts at: 4601 4601 Size: 6472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2203 4280 276 Size: 53 83 186 936 669 2077 2468 Fragments arranged by size: 2468 2077 936 669 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI