(Linear) (Six-base) MAPSORT of: KIEE5766.seq Check: 9955 from: 1 to: 1767 KIEE5766.orf With 182 enzymes: SgfI * December 5, 2014 13:10 .. AatII G_ACGT'C Cuts at: 0 200 1767 Size: 200 1567 AccI GT'mk_AC Cuts at: 0 574 1767 Size: 574 1193 Acc65I G'GTAC_C Cuts at: 0 478 1767 Size: 478 1289 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 409 518 881 1250 1396 1767 Size: 235 174 109 363 369 146 371 Fragments arranged by size: 371 369 363 235 174 146 109 AhdI GACnn_n'nnGTC Cuts at: 0 253 498 1767 Size: 253 245 1269 Fragments arranged by size: 1269 253 245 AlwNI CAG_nnn'CTG Cuts at: 0 1219 1767 Size: 1219 548 ApaI G_GGCC'C Cuts at: 0 508 1767 Size: 508 1259 ApoI r'AATT_y Cuts at: 0 67 88 544 1336 1711 1767 Size: 67 21 456 792 375 56 Fragments arranged by size: 792 456 375 67 56 21 AvaI C'yCGr_G Cuts at: 0 1633 1767 Size: 1633 134 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 640 673 1767 Size: 640 33 1094 Fragments arranged by size: 1094 640 33 BanI G'GyrC_C Cuts at: 0 478 1767 Size: 478 1289 BanII G_rGCy'C Cuts at: 0 508 1767 Size: 508 1259 BbsI GAAGACnn'nnnn_ Cuts at: 0 249 1384 1589 1767 Size: 249 1135 205 178 Fragments arranged by size: 1135 249 205 178 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 666 700 1767 Size: 666 34 1067 Fragments arranged by size: 1067 666 34 BciVI GTATCCnnnnn_n' Cuts at: 0 668 1767 Size: 668 1099 BclI T'GATC_A Cuts at: 0 1601 1767 Size: 1601 166 BfrBI ATG'CAT Cuts at: 0 1081 1767 Size: 1081 686 Bme1580I G_kGCm'C Cuts at: 0 508 1767 Size: 508 1259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 263 953 1767 Size: 118 145 690 814 Fragments arranged by size: 814 690 145 118 BsaBI GATnn'nnATC Cuts at: 0 1031 1048 1767 Size: 1031 17 719 Fragments arranged by size: 1031 719 17 BsaHI Gr'CG_yC Cuts at: 0 197 1767 Size: 197 1570 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1725 1767 Size: 1725 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1664 1767 Size: 1664 103 BsiEI CG_ry'CG Cuts at: 0 851 1767 Size: 851 916 BsiHKAI G_wGCw'C Cuts at: 0 465 1255 1767 Size: 465 790 512 Fragments arranged by size: 790 512 465 BsmI GAATG_Cn' Cuts at: 0 766 1767 Size: 766 1001 Bsp1286I G_dGCh'C Cuts at: 0 465 508 1255 1767 Size: 465 43 747 512 Fragments arranged by size: 747 512 465 43 BsrDI GCAATG_nn' Cuts at: 0 684 1767 Size: 684 1083 BstAPI GCAn_nnn'nTGC Cuts at: 0 469 1108 1767 Size: 469 639 659 Fragments arranged by size: 659 639 469 BstZ17I GTA'TAC Cuts at: 0 575 1767 Size: 575 1192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1653 1767 Size: 1653 114 ClaI AT'CG_AT Cuts at: 0 402 1767 Size: 402 1365 DraI TTT'AAA Cuts at: 0 1007 1767 Size: 1007 760 EarI CTCTTCn'nnn_ Cuts at: 0 37 329 1767 Size: 37 292 1438 Fragments arranged by size: 1438 292 37 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 235 263 409 518 881 953 1250 Size: 118 117 28 146 109 363 72 297 Cuts at: 1250 1396 1767 Size: 146 371 Fragments arranged by size: 371 363 297 146 146 118 117 109 72 28 EcoO109I rG'GnC_Cy Cuts at: 0 1618 1686 1767 Size: 1618 68 81 Fragments arranged by size: 1618 81 68 EcoRV GAT'ATC Cuts at: 0 540 1290 1767 Size: 540 750 477 Fragments arranged by size: 750 540 477 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 203 235 239 261 271 293 484 516 Size: 203 32 4 22 10 22 191 32 Cuts at: 516 597 629 1586 1618 1767 Size: 81 32 957 32 149 Fragments arranged by size: 957 203 191 149 81 32 32 32 32 22 22 10 4 HindIII A'AGCT_T Cuts at: 0 1678 1767 Size: 1678 89 KpnI G_GTAC'C Cuts at: 0 482 1767 Size: 482 1285 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 933 1081 1224 1734 1767 Size: 933 148 143 510 33 Fragments arranged by size: 933 510 148 143 33 MslI CAynn'nnrTG Cuts at: 0 1082 1132 1490 1767 Size: 1082 50 358 277 Fragments arranged by size: 1082 358 277 50 MspA1I CmG'CkG Cuts at: 0 1219 1333 1767 Size: 1219 114 434 Fragments arranged by size: 1219 434 114 NsiI A_TGCA'T Cuts at: 0 1083 1767 Size: 1083 684 NspI r_CATG'y Cuts at: 0 1085 1767 Size: 1085 682 PasI CC'CwG_GG Cuts at: 0 42 1767 Size: 42 1725 PflMI CCAn_nnn'nTGG Cuts at: 0 42 489 1767 Size: 42 447 1278 Fragments arranged by size: 1278 447 42 PpuMI rG'GwC_Cy Cuts at: 0 1686 1767 Size: 1686 81 PspOMI G'GGCC_C Cuts at: 0 504 1767 Size: 504 1263 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 356 388 762 794 1767 Size: 356 32 374 32 973 Fragments arranged by size: 973 374 356 32 32 PvuII CAG'CTG Cuts at: 0 1333 1767 Size: 1333 434 SapI GCTCTTCn'nnn_ Cuts at: 0 37 1767 Size: 37 1730 SfcI C'TryA_G Cuts at: 0 1479 1767 Size: 1479 288 SmaI CCC'GGG Cuts at: 0 1635 1767 Size: 1635 132 SspI AAT'ATT Cuts at: 0 447 750 829 1767 Size: 447 303 79 938 Fragments arranged by size: 938 447 303 79 StyI C'CwwG_G Cuts at: 0 566 1767 Size: 566 1201 XmaI C'CCGG_G Cuts at: 0 1633 1767 Size: 1633 134 ZraI GAC'GTC Cuts at: 0 198 1767 Size: 198 1569 Enzymes that do cut: AatII AccI Acc65I AcuI AhdI AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI BcgI BciVI BclI BfrBI Bme1580I BpmI BsaBI BsaHI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrDI BstAPI BstZ17I BtgZI ClaI DraI EarI Eco57MI EcoO109I EcoRV Hin4I HindIII KpnI MmeI MslI MspA1I NsiI NspI PasI PflMI PpuMI PspOMI PsrI PvuII SapI SfcI SmaI SspI StyI XmaI ZraI Enzymes that do not cut: AarI AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BbvCI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseYI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII HincII HpaI KasI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI