Restriction map for
pFN21AE5745
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI AvrII BbeI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
ClaI DraI Eam1105I Eco47III EcoO65I
EcoRV FbaI FseI HincII HindII
HindIII HpaI KasI MfeI MluI
MroI MunI NarI NdeI NruI
NspV PacI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SalI SbfI ScaI SfiI SfoI
SfuI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 431 GGTACC G^GTACC
AfaI 432 GTAC GT^AC
Alw44I 623 GTGCAC G^TGCAC
AlwNI 442 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 623 GTGCAC G^TGCAC
Asp718I 431 GGTACC G^GTACC
AspI 373 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 764 TGCGCA TGC^GCA
BamHI 1268 GGATCC G^GATCC
BbrPI 127 CACGTG CAC^GTG
BbsI 897 GAAGAC GAAGAC(2/6)
BlpI 810 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 810 GCTCAGC GC^T[ACGT]AGC
BpuAI 897 GAAGAC GAAGAC(2/6)
BsaBI 1269 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1780 GAGACC GGTCTC(1/5)
BsiEI 138 CGGCCG CG[GA][CT]^CG
BspMI 781 ACCTGC ACCTGC(4/8)
BsrBI 976 GAGCGG CCGCTC(-3/-3)
BsrI 1551 CCAGT ACTGG(1/-1)
BssHII 390 GCGCGC G^CGCGC
CelII 810 GCTCAGC GC^T[ACGT]AGC
CpoI 302 CGGTCCG CG^G[AT]CCG
DrdI 345 GACGCTGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 138 CGGCCG C^GGCCG
EclXI 138 CGGCCG C^GGCCG
Eco52I 138 CGGCCG C^GGCCG
EcoNI 231 CCTGTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1420 GAATTC G^AATTC
EcoT22I 1813 ATGCAT ATGCA^T
FspI 764 TGCGCA TGC^GCA
HaeII 443 AGCGCC [GA]GCGC^[CT]
KpnI 431 GGTACC GGTAC^C
KspI 1176 CCGCGG CCGC^GG
MamI 1269 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 735 GCCGGC GCC^GGC
NgoMIV 735 GCCGGC G^CCGGC
NheI 192 GCTAGC G^CTAGC
NotI 137 GCGGCCGC GC^GGCCGC
NsiI 1813 ATGCAT ATGCA^T
PaeR7I 1243 CTCGAG C^TCGAG
PflFI 373 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 127 CACGTG CAC^GTG
PmlI 127 CACGTG CAC^GTG
PstI 1749 CTGCAG CTGCA^G
PvuII 1342 CAGCTG CAG^CTG
RsaI 432 GTAC GT^AC
RsrII 302 CGGTCCG CG^G[AT]CCG
SacII 1176 CCGCGG CCGC^GG
SapI 480 GAAGAGC GCTCTTC(1/4)
SexAI 1721 ACCAGGT A^CC[AT]GGT
SgrAI 326 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 488 CCCGGG CCC^GGG
SphI 970 GCATGC GCATG^C
Tth111I 373 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1243 CTCGAG C^TCGAG
XmaCI 488 CCCGGG C^CCGGG
XmaI 488 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 437 GGCGTC G[GA]^CG[CT]C
751 GGCGTC
AocI 397 CCTCAGG CC^T[ACGT]AGG
1636 CCTCAGG
ApaI 657 GGGCCC GGGCC^C
1118 GGGCCC
AvaI 488 CCCGGG C^[CT]CG[GA]G
1243 CTCGAG
BanI 431 GGTACC G^G[CT][GA]CC
1255 GGCACC
BsaHI 437 GGCGTC G[GA]^CG[CT]C
751 GGCGTC
BsmBI 178 GAGACG CGTCTC(1/5)
333 GAGACG
BsoBI 488 CCCGGG C^[CT]CG[GA]G
1243 CTCGAG
BspLU11I 15 ACATGT A^CATGT
1360 ACATGT
BstAPI 776 GCAACACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1224 GCACAAGGTGC
BstYI 210 GGATCT [GA]^GATC[CT]
1268 GGATCC
Bsu36I 397 CCTCAGG CC^T[ACGT]AGG
1636 CCTCAGG
DraIII 1147 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1225 CACAAGGTG
EarI 1090 CTCTTC CTCTTC(1/4)
480 GAAGAG
Eco81I 397 CCTCAGG CC^T[ACGT]AGG
1636 CCTCAGG
Hin1I 437 GGCGTC G[GA]^CG[CT]C
751 GGCGTC
Ksp632I 1090 CTCTTC CTCTTC(1/4)
480 GAAGAG
MflI 210 GGATCT [GA]^GATC[CT]
1268 GGATCC
MslI 324 CACGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1488 CACTGAGATG
PpuMI 269 GGGACCT [GA]G^G[AT]CC[CT]
1406 GGGACCT
SacI 483 GAGCTC GAGCT^C
1027 GAGCTC
SfcI 1626 CTACAG C^T[GA][CT]AG
1749 CTGCAG
XhoII 210 GGATCT [GA]^GATC[CT]
1268 GGATCC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AflIII AluI
AlwI ApoI AspHI AvaII BalI
BanII BbvI BcnI BfaI BmyI
BpmI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I