(Circular) (Six-base) MAPSORT of: pFN21AE5745.seq Check: 7224 from: 1 to: 6625 pFN21AE5745 6625 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2795 2795 Size: 6625 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4435 278 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3968 1937 Size: 2031 4594 Acc65I G'GTAC_C Cuts at: 2436 3946 2436 Size: 1510 5115 AclI AA'CG_TT Cuts at: 1563 4752 5125 1563 Size: 3189 373 3063 Fragments arranged by size: 3189 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2300 4688 6072 6340 1632 Size: 668 2388 1384 268 1917 Fragments arranged by size: 2388 1917 1384 668 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6428 AflIII A'CryG_T Cuts at: 1203 2020 2223 3365 5524 6501 1203 Size: 817 203 1142 2159 977 1327 Fragments arranged by size: 2159 1327 1142 977 817 203 AgeI A'CCGG_T Cuts at: 5456 5456 Size: 6625 AhdI GACnn_n'nnGTC Cuts at: 5354 5354 Size: 6625 AleI CACnn'nnGTG Cuts at: 1166 2333 1166 Size: 1167 5458 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3779 1184 Size: 2595 4030 AlwNI CAG_nnn'CTG Cuts at: 2452 4368 5940 2452 Size: 1916 1572 3137 Fragments arranged by size: 3137 1916 1572 ApaI G_GGCC'C Cuts at: 2666 3127 2666 Size: 461 6164 ApaLI G'TGCA_C Cuts at: 2628 4682 5838 2628 Size: 2054 1156 3415 Fragments arranged by size: 3415 2054 1156 ApoI r'AATT_y Cuts at: 1493 3209 3425 3509 3934 4160 4312 6421 1493 Size: 1716 216 84 425 226 152 2109 1697 Fragments arranged by size: 2109 1716 1697 425 226 216 152 84 AseI AT'TA_AT Cuts at: 160 1032 5179 160 Size: 872 4147 1606 Fragments arranged by size: 4147 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 2493 3248 3950 1870 Size: 73 550 755 702 4545 Fragments arranged by size: 4545 755 702 550 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2428 2461 2428 Size: 33 6592 BamHI G'GATC_C Cuts at: 3273 3955 4322 3273 Size: 682 367 5576 Fragments arranged by size: 5576 682 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2436 3260 3946 5401 618 Size: 325 217 627 649 824 686 1455 1842 Fragments arranged by size: 1842 1455 824 686 649 627 325 217 BanII G_rGCy'C Cuts at: 1391 2492 2666 3036 3127 3405 3944 1391 Size: 1101 174 370 91 278 539 4072 Fragments arranged by size: 4072 1101 539 370 278 174 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2909 928 Size: 971 1010 4644 Fragments arranged by size: 4644 1010 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4816 4850 1903 Size: 34 2879 34 3678 Fragments arranged by size: 3678 2879 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4517 5727 4517 Size: 1210 5415 BclI T'GATC_A Cuts at: 1548 1548 Size: 6625 BfrBI ATG'CAT Cuts at: 3820 3820 Size: 6625 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5236 136 Size: 107 122 71 631 605 3564 1525 Fragments arranged by size: 3564 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6620 6620 Size: 6625 BlpI GC'TnA_GC Cuts at: 2816 4042 2816 Size: 1226 5399 Bme1580I G_kGCm'C Cuts at: 1790 2632 2666 2712 3127 3263 4686 5842 1790 Size: 842 34 46 415 136 1423 1156 2573 Fragments arranged by size: 2573 1423 1156 842 415 136 46 34 BmgBI CAC'GTC Cuts at: 1318 2342 2380 1318 Size: 1024 38 5563 Fragments arranged by size: 5563 1024 38 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4356 5314 442 Size: 416 53 83 399 24 2939 958 1753 Fragments arranged by size: 2939 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2201 1056 Size: 1145 5480 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2506 2506 Size: 6625 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 2558 2615 2792 2810 3050 5285 1239 Size: 1292 27 57 177 18 240 2235 2579 Fragments arranged by size: 2579 2235 1292 240 177 57 27 18 Bpu10I CC'TnA_GC Cuts at: 3841 3841 Size: 6625 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2105 2708 2974 4750 5615 5913 2105 Size: 603 266 1776 865 298 2817 Fragments arranged by size: 2817 1776 865 603 298 266 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3779 5288 882 Size: 617 2280 1509 2219 Fragments arranged by size: 2280 2219 1509 617 BsaAI yAC'GTr Cuts at: 493 1204 2134 2224 2251 4416 493 Size: 711 930 90 27 2165 2702 Fragments arranged by size: 2702 2165 930 711 90 27 BsaBI GATnn'nnATC Cuts at: 3278 6619 3278 Size: 3341 3284 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2443 2757 4432 4814 Size: 53 83 186 936 910 314 1675 382 Cuts at: 4814 5449 275 Size: 635 1451 Fragments arranged by size: 1675 1451 936 910 635 382 314 186 83 53 BsaWI w'CCGG_w Cuts at: 5058 5456 5730 5877 6288 5058 Size: 398 274 147 411 5395 Fragments arranged by size: 5395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2622 2652 2783 2813 3752 Size: 30 165 30 1219 30 131 30 939 Cuts at: 3752 3782 1178 Size: 30 4021 Fragments arranged by size: 4021 1219 939 165 131 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 2391 2543 2567 2612 3760 1186 Size: 1009 196 152 24 45 1148 4051 Fragments arranged by size: 4051 1148 1009 196 152 45 24 BseYI C'CCAG_C Cuts at: 5828 6324 5828 Size: 496 6129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2627 3071 3134 3212 3257 3641 1163 Size: 573 891 444 63 78 45 384 4147 Fragments arranged by size: 4147 891 573 444 384 78 63 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2146 4075 4836 5864 1540 Size: 275 186 145 1929 761 1028 2301 Fragments arranged by size: 2301 1929 1028 761 275 186 145 BsiHKAI G_wGCw'C Cuts at: 2129 2256 2492 2632 2817 3036 4686 4771 5842 Size: 127 236 140 185 219 1650 85 1071 Cuts at: 5842 2129 Size: 2912 Fragments arranged by size: 2912 1650 1071 236 219 185 140 127 85 BsmI GAATG_Cn' Cuts at: 4136 4229 4136 Size: 93 6532 BsmBI CGTCTCn'nnnn_ Cuts at: 2177 2332 2177 Size: 155 6470 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2256 2492 2632 2666 2712 2817 Size: 399 339 127 236 140 34 46 105 Cuts at: 2817 3036 3127 3263 3405 3944 4686 4771 5842 Size: 219 91 136 142 539 742 85 1071 Cuts at: 5842 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 539 399 339 236 219 142 140 136 127 105 91 85 46 34 BspEI T'CCGG_A Cuts at: 6288 6288 Size: 6625 BspHI T'CATG_A Cuts at: 4514 6244 4514 Size: 1730 4895 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2795 3980 844 Size: 1951 1185 3489 Fragments arranged by size: 3489 1951 1185 BsrBI CCG'CTC Cuts at: 1394 1823 2983 4512 1394 Size: 429 1160 1529 3507 Fragments arranged by size: 3507 1529 1160 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5120 5294 65 Size: 1183 3872 174 1396 Fragments arranged by size: 3872 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2332 2439 2740 5269 5456 1700 Size: 110 522 107 301 2529 187 2869 Fragments arranged by size: 2869 2529 522 301 187 110 107 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6625 BssHII G'CGCG_C Cuts at: 1925 2395 1925 Size: 470 6155 BssSI C'ACGA_G Cuts at: 4685 5697 4685 Size: 1012 5613 BstAPI GCAn_nnn'nTGC Cuts at: 794 2787 3235 794 Size: 1993 448 4184 Fragments arranged by size: 4184 1993 448 BstBI TT'CG_AA Cuts at: 5429 5429 Size: 6625 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6625 BstYI r'GATC_y Cuts at: 1972 2215 3273 3955 4322 4707 4724 6176 6262 Size: 243 1058 682 367 385 17 1452 86 Cuts at: 6262 6274 6620 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1058 682 385 367 346 243 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2403 3642 2403 Size: 1239 5386 BtgI C'CryG_G Cuts at: 513 1065 2004 2202 2361 2745 2940 3181 3738 Size: 552 939 198 159 384 195 241 557 Cuts at: 3738 513 Size: 3400 Fragments arranged by size: 3400 939 557 552 384 241 198 195 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5886 Fragments arranged by size: 5886 683 56 BtsI GCAGTG_nn' Cuts at: 3442 3816 4141 4935 4955 3442 Size: 374 325 794 20 5112 Fragments arranged by size: 5112 794 374 325 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6590 DraI TTT'AAA Cuts at: 3929 3929 Size: 6625 DraIII CAC_nnn'GTG Cuts at: 3157 3235 3157 Size: 78 6547 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2356 5632 809 Size: 507 1040 3276 1802 Fragments arranged by size: 3276 1802 1040 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2457 2604 Size: 54 1425 50 275 18 313 314 147 Cuts at: 2604 2742 2937 3504 3682 4072 4961 6590 8 Size: 138 195 567 178 390 889 1629 43 Fragments arranged by size: 1629 1425 889 567 390 314 313 275 195 178 147 138 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2143 4072 1537 Size: 275 331 1929 4090 Fragments arranged by size: 4090 1929 331 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2480 3101 4555 1353 Size: 1127 621 1454 3423 Fragments arranged by size: 3423 1454 1127 621 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2362 5212 5586 5732 387 Size: 1064 138 773 2850 374 146 1280 Fragments arranged by size: 2850 1280 1064 773 374 146 138 EcoICRI GAG'CTC Cuts at: 2490 3034 2490 Size: 544 6081 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2531 2558 2615 2792 2810 3050 Size: 393 668 231 27 57 177 18 240 Cuts at: 3050 4688 5285 6072 6340 1239 Size: 1638 597 787 268 1524 Fragments arranged by size: 1638 1524 787 668 597 393 268 240 231 177 57 27 18 EcoNI CCTnn'n_nnAGG Cuts at: 2240 2240 Size: 6625 EcoO109I rG'GnC_Cy Cuts at: 2275 2533 3009 3124 3412 3526 2275 Size: 258 476 115 288 114 5374 Fragments arranged by size: 5374 476 288 258 115 114 EcoRI G'AATT_C Cuts at: 3425 3934 4312 3425 Size: 509 378 5738 Fragments arranged by size: 5738 509 378 FspI TGC'GCA Cuts at: 2771 5131 2771 Size: 2360 4265 HaeII r_GCGC'y Cuts at: 1678 2452 5772 1678 Size: 774 3320 2531 Fragments arranged by size: 3320 2531 774 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3752 3784 5266 5298 5340 Size: 32 135 32 2180 32 1482 32 42 Cuts at: 5340 5372 1373 Size: 32 2626 Fragments arranged by size: 2626 2180 1482 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3969 4215 669 Size: 988 281 2031 246 3079 Fragments arranged by size: 3079 2031 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6625 HpaI GTT'AAC Cuts at: 4215 4215 Size: 6625 KpnI G_GTAC'C Cuts at: 2440 3950 2440 Size: 1510 5115 MfeI C'AATT_G Cuts at: 4224 4224 Size: 6625 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3002 5739 5923 1305 Size: 1697 2737 184 2007 Fragments arranged by size: 2737 2007 1697 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2459 2606 3506 10 Size: 54 1425 343 627 147 900 3129 Fragments arranged by size: 3129 1425 900 627 343 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 3497 4583 4942 5101 518 Size: 648 1167 1164 1086 359 159 2042 Fragments arranged by size: 2042 1167 1164 1086 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2518 3183 3349 4041 4718 5866 6111 1606 Size: 912 665 166 692 677 1148 245 2120 Fragments arranged by size: 2120 1148 912 692 677 665 245 166 NaeI GCC'GGC Cuts at: 2742 2742 Size: 6625 NcoI C'CATG_G Cuts at: 513 1065 2004 2202 2361 2745 3738 513 Size: 552 939 198 159 384 993 3400 Fragments arranged by size: 3400 993 939 552 384 198 159 NdeI CA'TA_TG Cuts at: 387 387 Size: 6625 NgoMIV G'CCGG_C Cuts at: 2740 2740 Size: 6625 NheI G'CTAG_C Cuts at: 1052 2197 1052 Size: 1145 5480 NotI GC'GGCC_GC Cuts at: 2143 4072 2143 Size: 1929 4696 NsiI A_TGCA'T Cuts at: 3822 3822 Size: 6625 NspI r_CATG'y Cuts at: 1132 1229 2024 2979 3369 3766 3983 5528 1132 Size: 97 795 955 390 397 217 1545 2229 Fragments arranged by size: 2229 1545 955 795 397 390 217 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6625 PciI A'CATG_T Cuts at: 2020 3365 5524 2020 Size: 1345 2159 3121 Fragments arranged by size: 3121 2159 1345 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6625 PfoI T'CCnGG_A Cuts at: 2211 3113 2211 Size: 902 5723 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PmlI CAC'GTG Cuts at: 2134 2134 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 6207 6239 4694 Size: 32 1481 32 5080 Fragments arranged by size: 5080 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 3412 2275 Size: 1137 5488 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6625 PsiI TTA'TAA Cuts at: 4195 4195 Size: 6625 PspOMI G'GGCC_C Cuts at: 2662 3123 2662 Size: 461 6164 PspXI vC'TCGA_Gb Cuts at: 3248 3248 Size: 6625 PstI C_TGCA'G Cuts at: 830 3758 3977 830 Size: 2928 219 3478 Fragments arranged by size: 3478 2928 219 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 PvuII CAG'CTG Cuts at: 3349 3349 Size: 6625 RsrII CG'GwC_CG Cuts at: 2308 2308 Size: 6625 SacI G_AGCT'C Cuts at: 2492 3036 2492 Size: 544 6081 SacII CC_GC'GG Cuts at: 3184 3184 Size: 6625 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SapI GCTCTTCn'nnn_ Cuts at: 2480 2480 Size: 6625 SbfI CC_TGCA'GG Cuts at: 3977 3977 Size: 6625 ScaI AGT'ACT Cuts at: 4873 6585 4873 Size: 1712 4913 SexAI A'CCwGG_T Cuts at: 3726 3726 Size: 6625 SfcI C'TryA_G Cuts at: 826 1045 3631 3754 3973 5108 5789 5980 826 Size: 219 2586 123 219 1135 681 191 1471 Fragments arranged by size: 2586 1471 1135 681 219 219 191 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 2332 1700 Size: 632 5993 SmaI CCC'GGG Cuts at: 1872 2495 3952 1872 Size: 623 1457 4545 Fragments arranged by size: 4545 1457 623 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 2723 2953 3248 4729 5630 Size: 197 926 177 603 230 295 1481 901 Cuts at: 5630 5892 820 Size: 262 1553 Fragments arranged by size: 1553 1481 926 901 603 295 262 230 197 177 SnaBI TAC'GTA Cuts at: 493 4416 493 Size: 3923 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6625 SphI G_CATG'C Cuts at: 1132 2979 3983 1132 Size: 1847 1004 3774 Fragments arranged by size: 3774 1847 1004 SspI AAT'ATT Cuts at: 5 52 4549 5 Size: 47 4497 2081 Fragments arranged by size: 4497 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6625 StyI C'CwwG_G Cuts at: 513 1065 2004 2202 2361 2745 3738 4064 513 Size: 552 939 198 159 384 993 326 3074 Fragments arranged by size: 3074 993 939 552 384 326 198 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4652 4669 4822 5007 5452 325 Size: 1255 3072 17 153 185 445 1498 Fragments arranged by size: 3072 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4871 6583 96 Size: 274 80 33 51 1443 2894 1712 138 Fragments arranged by size: 2894 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2622 2654 3258 3290 6585 6617 2622 Size: 32 604 32 3295 32 2630 Fragments arranged by size: 3295 2630 604 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2381 1316 Size: 1065 5560 XbaI T'CTAG_A Cuts at: 3961 3961 Size: 6625 XhoI C'TCGA_G Cuts at: 1943 3248 1943 Size: 1305 5320 XmaI C'CCGG_G Cuts at: 1870 2493 3950 1870 Size: 623 1457 4545 Fragments arranged by size: 4545 1457 623 XmnI GAAnn'nnTTC Cuts at: 4754 4754 Size: 6625 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4433 276 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI PacI PsrI SanDI SfiI SfoI SrfI SwaI XcmI