################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccII AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsiWI BsmBI BsmI Bsp1407I BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CfoI Cfr10I CpoI DraI DraII DrdI Eam1105I Eco47III EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HpaI KasI KpnI KspI MamI MluI MluNI MscI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 1418 GTCTAC GT^[AC][GT]AC AccIII 161 TCCGGA T^CCGGA AclI 739 AACGTT AA^CGTT AcyI 36 GGCGTC G[GA]^CG[CT]C AfaI 392 GTAC GT^AC AocI 26 CCTCAGG CC^T[ACGT]AGG AseI 1240 ATTAAT AT^TAAT AsnI 1240 ATTAAT AT^TAAT AspHI 55 GAGCTC G[AT]GC[AT]^C AvaI 1403 CCCGAG C^[CT]CG[GA]G AvaII 597 GGTCC G^G[AT]CC BanI 1822 GGTGCC G^G[CT][GA]CC BanII 55 GAGCTC G[GA]GC[CT]^C BbsI 1796 GTCTTC GAAGAC(2/6) BlpI 52 GCTGAGC GC^T[ACGT]AGC BmyI 55 GAGCTC G[AGT]GC[ACT]^C Bpu1102I 52 GCTGAGC GC^T[ACGT]AGC BpuAI 1796 GTCTTC GAAGAC(2/6) BsaHI 36 GGCGTC G[GA]^CG[CT]C BsaI 76 GAGACC GGTCTC(1/5) BseAI 161 TCCGGA T^CCGGA BsiHKAI 55 GAGCTC G[AT]GC[AT]^C BsmFI 39 GTCCC GGGAC(10/14) BsoBI 1403 CCCGAG C^[CT]CG[GA]G Bsp1286I 55 GAGCTC G[AGT]GC[ACT]^C BspDI 869 ATCGAT AT^CGAT BspEI 161 TCCGGA T^CCGGA BspMI 381 ACCTGC ACCTGC(4/8) Bsu36I 26 CCTCAGG CC^T[ACGT]AGG CelII 52 GCTGAGC GC^T[ACGT]AGC ClaI 869 ATCGAT AT^CGAT DraIII 727 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 47 CCGTGG C^C[GA][CT]GG Eco81I 26 CCTCAGG CC^T[ACGT]AGG EcoNI 199 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1058 ATGCAT ATGCA^T HgaI 37 GCGTC GACGC(5/10) Hin1I 36 GGCGTC G[GA]^CG[CT]C MaeII 740 ACGT A^CGT MfeI 939 CAATTG C^AATTG MroI 161 TCCGGA T^CCGGA MunI 939 CAATTG C^AATTG NotI 7 GCGGCCGC GC^GGCCGC NsiI 1058 ATGCAT ATGCA^T PshAI 1014 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1240 ATTAAT AT^TAAT Psp1406I 739 AACGTT AA^CGTT PstI 383 CTGCAG CTGCA^G RsaI 392 GTAC GT^AC SacI 55 GAGCTC GAGCT^C SapI 565 GCTCTTC GCTCTTC(1/4) SspI 1492 AATATT AAT^ATT StuI 89 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Asp700I 517 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 718 GAATTTTTTC AspI 768 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1003 GACCATGTC BcgI 1168 CGATATTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 294 GCAAAAAATTCG BcnI 42 CCCGG CC^[GC]GG 72 CCCGG BsaWI 161 TCCGGA [AT]^CCGG[AT] 594 TCCGGT BsgI 1048 CTGCAC GTGCAG(16/14) 747 GTGCAG BsiEI 8 CGGCCG CG[GA][CT]^CG 44 CGGCCG BsmAI 76 GAGAC GTCTC(1/5) 1232 GAGAC BstNI 1354 CCTGG CC^[AT]GG 1655 CCTGG BstYI 154 AGATCC [GA]^GATC[CT] 471 AGATCC Cac8I 853 GCCTGC GC[ACGT]^[ACGT]GC 979 GCTCGC EaeI 8 CGGCCG [CT]^GGCC[GA] 44 CGGCCG EagI 8 CGGCCG C^GGCCG 44 CGGCCG EclXI 8 CGGCCG C^GGCCG 44 CGGCCG Eco52I 8 CGGCCG C^GGCCG 44 CGGCCG EcoRII 1354 CCTGG ^CC[AT]GG 1655 CCTGG MflI 154 AGATCC [GA]^GATC[CT] 471 AGATCC MvaI 1354 CCTGG CC^[AT]GG 1655 CCTGG NciI 42 CCCGG CC^[GC]GG 72 CCCGG NlaIV 449 GGAACC GG[ACGT]^[ACGT]CC 1822 GGTGCC NspI 540 GCATGT [GA]CATG^[CT] 1060 GCATGT PflFI 768 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1003 GACCATGTC PleI 191 GACTC GAGTC(4/5) 502 GACTC PvuII 289 CAGCTG CAG^CTG 1891 CAGCTG SfaNI 850 GATGC GCATC(5/9) 1057 GATGC TfiI 872 GATTC G^A[AT]TC 1573 GAATC Tth111I 768 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1003 GACCATGTC XbaI 513 TCTAGA T^CTAGA 775 TCTAGA XhoII 154 AGATCC [GA]^GATC[CT] 471 AGATCC XmnI 517 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 718 GAATTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbvI BfaI BpmI BsaJI BseRI BsiYI BslI BsrI BssKI BstF5I Cfr13I DdeI DpnII EarI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I