(Circular) (Six-base) MAPSORT of: pFN21AE5707.seq Check: 709 from: 1 to: 6667 pFN21AE5707 6667 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4477 278 Size: 53 83 186 936 2941 2468 Fragments arranged by size: 2941 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3424 4010 1937 Size: 1487 586 4594 Fragments arranged by size: 4594 1487 586 Acc65I G'GTAC_C Cuts at: 3988 3988 Size: 6667 AclI AA'CG_TT Cuts at: 1563 2745 4794 5167 1563 Size: 1182 2049 373 3063 Fragments arranged by size: 3063 2049 1182 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2268 2513 2558 2837 3855 4730 6114 6382 Size: 636 245 45 279 1018 875 1384 268 Cuts at: 6382 1632 Size: 1917 Fragments arranged by size: 1917 1384 1018 875 636 279 268 245 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6470 AflIII A'CryG_T Cuts at: 1203 5566 6543 1203 Size: 4363 977 1327 Fragments arranged by size: 4363 1327 977 AgeI A'CCGG_T Cuts at: 5498 5498 Size: 6667 AhdI GACnn_n'nnGTC Cuts at: 5396 5396 Size: 6667 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6667 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3078 1184 Size: 1894 4773 AlwNI CAG_nnn'CTG Cuts at: 4410 5982 4410 Size: 1572 5095 ApaLI G'TGCA_C Cuts at: 4724 5880 4724 Size: 1156 5511 ApoI r'AATT_y Cuts at: 1493 2304 2723 3332 3953 3976 4202 4354 6463 Size: 811 419 609 621 23 226 152 2109 Cuts at: 6463 1493 Size: 1697 Fragments arranged by size: 2109 1697 811 621 609 419 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 3246 5221 160 Size: 872 2214 1975 1606 Fragments arranged by size: 2214 1975 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6667 AvaI C'yCGr_G Cuts at: 1870 1943 3408 3992 1870 Size: 73 1465 584 4545 Fragments arranged by size: 4545 1465 584 73 BamHI G'GATC_C Cuts at: 3997 4364 3997 Size: 367 6300 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3827 3988 5443 618 Size: 325 217 627 2040 161 1455 1842 Fragments arranged by size: 2040 1842 1455 627 325 217 161 BanII G_rGCy'C Cuts at: 1391 2064 3986 1391 Size: 673 1922 4072 Fragments arranged by size: 4072 1922 673 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3794 928 Size: 971 1895 3801 Fragments arranged by size: 3801 1895 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2288 2322 3162 3196 4858 4892 1903 Size: 34 351 34 840 34 1662 34 3678 Fragments arranged by size: 3678 1662 840 351 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4559 5769 4559 Size: 1210 5457 BclI T'GATC_A Cuts at: 1548 1548 Size: 6667 BfrBI ATG'CAT Cuts at: 3065 3065 Size: 6667 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5278 136 Size: 107 122 71 631 605 337 3269 1525 Fragments arranged by size: 3269 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6662 6662 Size: 6667 BlpI GC'TnA_GC Cuts at: 2058 4084 2058 Size: 2026 4641 Bme1580I G_kGCm'C Cuts at: 1790 4728 5884 1790 Size: 2938 1156 2573 Fragments arranged by size: 2938 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6667 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2424 2743 2796 Size: 416 53 83 399 24 1007 319 53 Cuts at: 2796 4398 5356 442 Size: 1602 958 1753 Fragments arranged by size: 1753 1602 1007 958 416 399 319 83 53 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6667 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2717 3290 3381 5327 1239 Size: 1478 573 91 1946 2579 Fragments arranged by size: 2579 1946 1478 573 91 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4792 5657 5955 4792 Size: 865 298 5504 Fragments arranged by size: 5504 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2075 5330 882 Size: 617 576 3255 2219 Fragments arranged by size: 3255 2219 617 576 BsaAI yAC'GTr Cuts at: 493 1204 4458 493 Size: 711 3254 2702 Fragments arranged by size: 3254 2702 711 BsaBI GATnn'nnATC Cuts at: 6661 6661 Size: 6667 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 4474 4856 5491 Size: 53 83 186 936 509 2432 382 635 Cuts at: 5491 275 Size: 1451 Fragments arranged by size: 2432 1451 936 635 509 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2166 2599 5100 5498 5772 5919 6330 2166 Size: 433 2501 398 274 147 411 2503 Fragments arranged by size: 2503 2501 433 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2570 2600 1178 Size: 30 165 30 1167 30 5245 Fragments arranged by size: 5245 1167 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2020 2226 3879 1186 Size: 834 206 1653 3974 Fragments arranged by size: 3974 1653 834 206 BseYI C'CCAG_C Cuts at: 2400 3894 5870 6366 2400 Size: 1494 1976 496 2701 Fragments arranged by size: 2701 1976 1494 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2773 3038 1163 Size: 573 1037 265 4792 Fragments arranged by size: 4792 1037 573 265 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 2052 4117 4878 5906 1540 Size: 275 186 15 36 2065 761 1028 2301 Fragments arranged by size: 2301 2065 1028 761 275 186 36 15 BsiHKAI G_wGCw'C Cuts at: 2064 4728 4813 5884 2064 Size: 2664 85 1071 2847 Fragments arranged by size: 2847 2664 1071 85 BsmI GAATG_Cn' Cuts at: 4178 4271 4178 Size: 93 6574 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 3986 4728 4813 5884 1391 Size: 399 274 1922 742 85 1071 2174 Fragments arranged by size: 2174 1922 1071 742 399 274 85 BspEI T'CCGG_A Cuts at: 2166 6330 2166 Size: 4164 2503 BspHI T'CATG_A Cuts at: 4556 6286 4556 Size: 1730 4937 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2395 4022 844 Size: 1551 1627 3489 Fragments arranged by size: 3489 1627 1551 BsrBI CCG'CTC Cuts at: 1394 1823 4554 1394 Size: 429 2731 3507 Fragments arranged by size: 3507 2731 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5162 5336 65 Size: 1183 3914 174 1396 Fragments arranged by size: 3914 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5311 5498 1700 Size: 110 3501 187 2869 Fragments arranged by size: 3501 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6667 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6667 BssSI C'ACGA_G Cuts at: 4727 5739 4727 Size: 1012 5655 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6667 BstBI TT'CG_AA Cuts at: 5471 5471 Size: 6667 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6667 BstYI r'GATC_y Cuts at: 1972 2159 2476 3997 4364 4749 4766 6218 6304 Size: 187 317 1521 367 385 17 1452 86 Cuts at: 6304 6316 6662 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1521 1452 385 367 346 317 187 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2032 2032 Size: 6667 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 513 Size: 552 939 48 5128 Fragments arranged by size: 5128 939 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5928 Fragments arranged by size: 5928 683 56 BtsI GCAGTG_nn' Cuts at: 4183 4977 4997 4183 Size: 794 20 5853 Fragments arranged by size: 5853 794 20 ClaI AT'CG_AT Cuts at: 1554 2875 1554 Size: 1321 5346 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2746 2781 2817 2852 529 Size: 35 2182 35 36 35 4344 Fragments arranged by size: 4344 2182 36 35 35 35 DraI TTT'AAA Cuts at: 3971 3971 Size: 6667 DraIII CAC_nnn'GTG Cuts at: 2737 2737 Size: 6667 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5674 809 Size: 507 4358 1802 Fragments arranged by size: 4358 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2049 4114 Size: 54 1425 50 275 18 183 36 2065 Cuts at: 4114 5003 6632 8 Size: 889 1629 43 Fragments arranged by size: 2065 1629 1425 889 275 183 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 2049 4114 1537 Size: 275 201 36 2065 4090 Fragments arranged by size: 4090 2065 275 201 36 EarI CTCTTCn'nnn_ Cuts at: 1353 2241 2577 3186 4597 1353 Size: 888 336 609 1411 3423 Fragments arranged by size: 3423 1411 888 609 336 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5254 5628 5774 387 Size: 1064 138 3665 374 146 1280 Fragments arranged by size: 3665 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2062 2062 Size: 6667 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2268 2513 2558 2717 2837 3290 3381 Size: 393 636 245 45 159 120 453 91 Cuts at: 3381 3855 4730 5327 6114 6382 1239 Size: 474 875 597 787 268 1524 Fragments arranged by size: 1524 875 787 636 597 474 453 393 268 245 159 120 91 45 EcoNI CCTnn'n_nnAGG Cuts at: 2208 2208 Size: 6667 EcoRI G'AATT_C Cuts at: 3976 4354 3976 Size: 378 6289 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3248 3248 Size: 6667 FspI TGC'GCA Cuts at: 5173 5173 Size: 6667 HaeII r_GCGC'y Cuts at: 1678 5814 1678 Size: 4136 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 3662 3694 5308 Size: 32 135 32 896 32 1162 32 1614 Cuts at: 5308 5340 5382 5414 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1614 1162 896 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4011 4257 669 Size: 988 281 2073 246 3079 Fragments arranged by size: 3079 2073 988 281 246 HindIII A'AGCT_T Cuts at: 748 2280 3119 3910 748 Size: 1532 839 791 3505 Fragments arranged by size: 3505 1532 839 791 HpaI GTT'AAC Cuts at: 4257 4257 Size: 6667 KpnI G_GTAC'C Cuts at: 3992 3992 Size: 6667 MfeI C'AATT_G Cuts at: 2944 4266 2944 Size: 1322 5345 MluI A'CGCG_T Cuts at: 6543 6543 Size: 6667 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2559 5781 5965 1305 Size: 1254 3222 184 2007 Fragments arranged by size: 3222 2007 1254 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4845 Fragments arranged by size: 4845 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2850 3060 3810 4625 4984 5143 518 Size: 648 1684 210 750 815 359 159 2042 Fragments arranged by size: 2042 1684 815 750 648 359 210 159 MspA1I CmG'CkG Cuts at: 1606 2296 3046 3898 4083 4760 5908 6153 1606 Size: 690 750 852 185 677 1148 245 2120 Fragments arranged by size: 2120 1148 852 750 690 677 245 185 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5176 Fragments arranged by size: 5176 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6667 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6667 NotI GC'GGCC_GC Cuts at: 2013 4114 2013 Size: 2101 4566 NsiI A_TGCA'T Cuts at: 3067 3067 Size: 6667 NspI r_CATG'y Cuts at: 1132 1229 2549 3069 4025 5570 1132 Size: 97 1320 520 956 1545 2229 Fragments arranged by size: 2229 1545 1320 956 520 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6667 PciI A'CATG_T Cuts at: 5566 5566 Size: 6667 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6667 PfoI T'CCnGG_A Cuts at: 3659 3659 Size: 6667 PmeI GTTT'AAAC Cuts at: 3971 3971 Size: 6667 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4736 4768 6249 6281 4736 Size: 32 1481 32 5122 Fragments arranged by size: 5122 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3023 1613 Size: 1410 5257 PsiI TTA'TAA Cuts at: 3950 4237 3950 Size: 287 6380 PstI C_TGCA'G Cuts at: 830 2392 4019 830 Size: 1562 1627 3478 Fragments arranged by size: 3478 1627 1562 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6667 PvuII CAG'CTG Cuts at: 2296 3898 2296 Size: 1602 5065 SacI G_AGCT'C Cuts at: 2064 2064 Size: 6667 SalI G'TCGA_C Cuts at: 1936 4009 1936 Size: 2073 4594 SapI GCTCTTCn'nnn_ Cuts at: 2577 2577 Size: 6667 SbfI CC_TGCA'GG Cuts at: 4019 4019 Size: 6667 ScaI AGT'ACT Cuts at: 4915 6627 4915 Size: 1712 4955 SfcI C'TryA_G Cuts at: 826 1045 2388 2970 3425 4015 5150 5831 6022 Size: 219 1343 582 455 590 1135 681 191 Cuts at: 6022 826 Size: 1471 Fragments arranged by size: 1471 1343 1135 681 590 582 455 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6667 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6667 SmaI CCC'GGG Cuts at: 1872 3994 1872 Size: 2122 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4771 5672 5934 820 Size: 197 926 2828 901 262 1553 Fragments arranged by size: 2828 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4458 493 Size: 3965 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6667 SphI G_CATG'C Cuts at: 1132 4025 1132 Size: 2893 3774 SspI AAT'ATT Cuts at: 5 52 3499 4591 5 Size: 47 3447 1092 2081 Fragments arranged by size: 3447 2081 1092 47 StuI AGG'CCT Cuts at: 1517 2096 1517 Size: 579 6088 StyI C'CwwG_G Cuts at: 513 1065 2004 4106 513 Size: 552 939 2102 3074 Fragments arranged by size: 3074 2102 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2725 3908 4694 4711 4864 5049 5494 Size: 1255 1145 1183 786 17 153 185 445 Cuts at: 5494 325 Size: 1498 Fragments arranged by size: 1498 1255 1183 1145 786 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4913 6625 96 Size: 274 80 33 51 1443 2936 1712 138 Fragments arranged by size: 2936 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6627 6659 6627 Size: 32 6635 Tth111I GACn'n_nGTC Cuts at: 1316 2776 3011 1316 Size: 1460 235 4972 Fragments arranged by size: 4972 1460 235 XbaI T'CTAG_A Cuts at: 2518 2780 4003 2518 Size: 262 1223 5182 Fragments arranged by size: 5182 1223 262 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6667 XmaI C'CCGG_G Cuts at: 1870 3992 1870 Size: 2122 4545 XmnI GAAnn'nnTTC Cuts at: 2526 2727 4796 2526 Size: 201 2069 4397 Fragments arranged by size: 4397 2069 201 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4475 276 Size: 53 83 186 936 2941 2468 Fragments arranged by size: 2941 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI