(Linear) (Six-base) MAPSORT of: KIEE5707.seq Check: 5453 from: 1 to: 1962 KIEE5707.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AccI GT'mk_AC Cuts at: 0 1419 1962 Size: 1419 543 AclI AA'CG_TT Cuts at: 0 740 1962 Size: 740 1222 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 263 508 553 832 1850 1962 Size: 263 245 45 279 1018 112 Fragments arranged by size: 1018 279 263 245 112 45 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1073 1962 Size: 1073 889 ApoI r'AATT_y Cuts at: 0 299 718 1327 1948 1962 Size: 299 419 609 621 14 Fragments arranged by size: 621 609 419 299 14 AseI AT'TA_AT Cuts at: 0 1241 1962 Size: 1241 721 AvaI C'yCGr_G Cuts at: 0 1403 1962 Size: 1403 559 BanI G'GyrC_C Cuts at: 0 1822 1962 Size: 1822 140 BanII G_rGCy'C Cuts at: 0 59 1962 Size: 59 1903 BbsI GAAGACnn'nnnn_ Cuts at: 0 1789 1962 Size: 1789 173 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 283 317 1157 1191 1962 Size: 283 34 840 34 771 Fragments arranged by size: 840 771 283 34 34 BfrBI ATG'CAT Cuts at: 0 1060 1962 Size: 1060 902 BlpI GC'TnA_GC Cuts at: 0 53 1962 Size: 53 1909 BmrI ACTGGGnnnn_n' Cuts at: 0 419 738 791 1962 Size: 419 319 53 1171 Fragments arranged by size: 1171 419 319 53 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 1285 1376 1962 Size: 712 573 91 586 Fragments arranged by size: 712 586 573 91 BsaI GGTCTCn'nnnn_ Cuts at: 0 70 1962 Size: 70 1892 BsaHI Gr'CG_yC Cuts at: 0 37 1962 Size: 37 1925 BsaWI w'CCGG_w Cuts at: 0 161 594 1962 Size: 161 433 1368 Fragments arranged by size: 1368 433 161 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 565 595 1962 Size: 565 30 1367 Fragments arranged by size: 1367 565 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 15 221 1874 1962 Size: 15 206 1653 88 Fragments arranged by size: 1653 206 88 15 BseYI C'CCAG_C Cuts at: 0 395 1889 1962 Size: 395 1494 73 Fragments arranged by size: 1494 395 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 768 1033 1962 Size: 768 265 929 Fragments arranged by size: 929 768 265 BsiEI CG_ry'CG Cuts at: 0 11 47 1962 Size: 11 36 1915 Fragments arranged by size: 1915 36 11 BsiHKAI G_wGCw'C Cuts at: 0 59 1962 Size: 59 1903 Bsp1286I G_dGCh'C Cuts at: 0 59 1962 Size: 59 1903 BspEI T'CCGG_A Cuts at: 0 161 1962 Size: 161 1801 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 390 1962 Size: 390 1572 BstYI r'GATC_y Cuts at: 0 154 471 1962 Size: 154 317 1491 Fragments arranged by size: 1491 317 154 Bsu36I CC'TnA_GG Cuts at: 0 27 1962 Size: 27 1935 BtgI C'CryG_G Cuts at: 0 47 1962 Size: 47 1915 ClaI AT'CG_AT Cuts at: 0 870 1962 Size: 870 1092 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 741 776 812 847 1962 Size: 741 35 36 35 1115 Fragments arranged by size: 1115 741 36 35 35 DraIII CAC_nnn'GTG Cuts at: 0 732 1962 Size: 732 1230 EaeI y'GGCC_r Cuts at: 0 8 44 1962 Size: 8 36 1918 Fragments arranged by size: 1918 36 8 EagI C'GGCC_G Cuts at: 0 8 44 1962 Size: 8 36 1918 Fragments arranged by size: 1918 36 8 EarI CTCTTCn'nnn_ Cuts at: 0 236 572 1181 1962 Size: 236 336 609 781 Fragments arranged by size: 781 609 336 236 EcoICRI GAG'CTC Cuts at: 0 57 1962 Size: 57 1905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 263 508 553 712 832 1285 1376 1850 Size: 263 245 45 159 120 453 91 474 Cuts at: 1850 1962 Size: 112 Fragments arranged by size: 474 453 263 245 159 120 112 91 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 203 1962 Size: 203 1759 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1243 1962 Size: 1243 719 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 463 495 1657 1689 1962 Size: 463 32 1162 32 273 Fragments arranged by size: 1162 463 273 32 32 HindIII A'AGCT_T Cuts at: 0 275 1114 1905 1962 Size: 275 839 791 57 Fragments arranged by size: 839 791 275 57 MfeI C'AATT_G Cuts at: 0 939 1962 Size: 939 1023 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 554 1962 Size: 554 1408 MslI CAynn'nnrTG Cuts at: 0 845 1055 1805 1962 Size: 845 210 750 157 Fragments arranged by size: 845 750 210 157 MspA1I CmG'CkG Cuts at: 0 291 1041 1893 1962 Size: 291 750 852 69 Fragments arranged by size: 852 750 291 69 NotI GC'GGCC_GC Cuts at: 0 8 1962 Size: 8 1954 NsiI A_TGCA'T Cuts at: 0 1062 1962 Size: 1062 900 NspI r_CATG'y Cuts at: 0 544 1064 1962 Size: 544 520 898 Fragments arranged by size: 898 544 520 PfoI T'CCnGG_A Cuts at: 0 1654 1962 Size: 1654 308 PshAI GACnn'nnGTC Cuts at: 0 1018 1962 Size: 1018 944 PsiI TTA'TAA Cuts at: 0 1945 1962 Size: 1945 17 PstI C_TGCA'G Cuts at: 0 387 1962 Size: 387 1575 PvuII CAG'CTG Cuts at: 0 291 1893 1962 Size: 291 1602 69 Fragments arranged by size: 1602 291 69 SacI G_AGCT'C Cuts at: 0 59 1962 Size: 59 1903 SapI GCTCTTCn'nnn_ Cuts at: 0 572 1962 Size: 572 1390 SfcI C'TryA_G Cuts at: 0 383 965 1420 1962 Size: 383 582 455 542 Fragments arranged by size: 582 542 455 383 SspI AAT'ATT Cuts at: 0 1494 1962 Size: 1494 468 StuI AGG'CCT Cuts at: 0 91 1962 Size: 91 1871 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 720 1903 1962 Size: 720 1183 59 Fragments arranged by size: 1183 720 59 Tth111I GACn'n_nGTC Cuts at: 0 771 1006 1962 Size: 771 235 956 Fragments arranged by size: 956 771 235 XbaI T'CTAG_A Cuts at: 0 513 775 1962 Size: 513 262 1187 Fragments arranged by size: 1187 513 262 XmnI GAAnn'nnTTC Cuts at: 0 521 722 1962 Size: 521 201 1240 Fragments arranged by size: 1240 521 201 Enzymes that do cut: AccI AclI AcuI AlfI ApoI AseI AvaI BanI BanII BbsI BcgI BfrBI BlpI BmrI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BstYI Bsu36I BtgI ClaI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI FalI Hin4I HindIII MfeI MmeI MslI MspA1I NotI NsiI NspI PfoI PshAI PsiI PstI PvuII SacI SapI SfcI SspI StuI TaqII Tth111I XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BglI BglII Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI DraI DrdI EciI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StyI SwaI TatI TstI XcmI XhoI XmaI ZraI