################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AccII AclI AflII AflIII AgeI AscI AseI AsnI AspI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI EagI EarI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XcmI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 456 GACGTC GACGT^C AccI 649 GTCTAC GT^[AC][GT]AC AccIII 146 TCCGGA T^CCGGA AcsI 163 GAATTT [GA]^AATT[CT] AcyI 456 GACGTC G[GA]^CG[CT]C AhdI 433 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1168 GTGCAC G^TGCAC AocI 1068 CCTCAGG CC^T[ACGT]AGG Aor51HI 1433 AGCGCT AGC^GCT ApaI 878 GGGCCC GGGCC^C ApaLI 1168 GTGCAC G^TGCAC ApoI 163 GAATTT [GA]^AATT[CT] Asp700I 907 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 433 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 668 CCCGGG C^[CT]CG[GA]G BalI 129 TGGCCA TGG^CCA BsaHI 456 GACGTC G[GA]^CG[CT]C BsaWI 146 TCCGGA [AT]^CCGG[AT] BseAI 146 TCCGGA T^CCGGA BsiEI 1215 CGATCG CG[GA][CT]^CG BsmFI 353 GGGAC GGGAC(10/14) BsoBI 668 CCCGGG C^[CT]CG[GA]G BspEI 146 TCCGGA T^CCGGA BspMI 584 ACCTGC ACCTGC(4/8) BsrDI 42 CATTGC GCAATG(2/0) BstAPI 579 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1068 CCTCAGG CC^T[ACGT]AGG EaeI 129 TGGCCA [CT]^GGCC[GA] Eam1105I 433 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1433 AGCGCT AGC^GCT Eco81I 1068 CCTCAGG CC^T[ACGT]AGG FauI 1428 CCCGC CCCGC(4/6) HaeII 1433 AGCGCT [GA]GCGC^[CT] Hin1I 456 GACGTC G[GA]^CG[CT]C HincII 1423 GTTGAC GT[CT]^[GA]AC HindII 1423 GTTGAC GT[CT]^[GA]AC MaeII 457 ACGT A^CGT MaeIII 1372 GTCAC ^GT[ACGT]AC MluNI 129 TGGCCA TGG^CCA MroI 146 TCCGGA T^CCGGA MscI 129 TGGCCA TGG^CCA MseI 416 TTAA T^TAA MslI 190 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 536 CAGCTG C[AC]G^C[GT]G NspI 770 ACATGC [GA]CATG^[CT] PvuI 1215 CGATCG CGAT^CG PvuII 536 CAGCTG CAG^CTG SacI 26 GAGCTC GAGCT^C ScaI 224 AGTACT AGT^ACT SmaI 668 CCCGGG CCC^GGG SmlI 268 CTCAAG C^T[CT][GA]AG Tru9I 416 TTAA T^TAA Tsp45I 1372 GTCAC ^GT[GC]AC Tsp509I 164 AATT ^AATT XbaI 1017 TCTAGA T^CTAGA XmaCI 668 CCCGGG C^CCGGG XmaI 668 CCCGGG C^CCGGG XmnI 907 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 112 GGTACC G^GTACC 798 GGTACC AlwI 1275 GATCC GGATC(4/5) 411 GGATC Asp718I 112 GGTACC G^GTACC 798 GGTACC AspHI 26 GAGCTC G[AT]GC[AT]^C 1168 GTGCAC BbsI 1084 GAAGAC GAAGAC(2/6) 1204 GTCTTC BbvCI 803 CCTCAGC CCTCAGC(-5/-2) 1047 GCTGAGG BciVI 49 GGATAC GTATCC(6/5) 334 GGATAC BcnI 668 CCCGG CC^[GC]GG 669 CCGGG BpuAI 1084 GAAGAC GAAGAC(2/6) 1204 GTCTTC BsaI 421 GAGACC GGTCTC(1/5) 964 GAGACC BseRI 1066 CTCCTC GAGGAG(10/8) 1033 GAGGAG BsgI 1055 CTGCAC GTGCAG(16/14) 208 GTGCAG BsiHKAI 26 GAGCTC G[AT]GC[AT]^C 1168 GTGCAC BsmBI 700 CGTCTC CGTCTC(1/5) 1036 GAGACG BspHI 830 TCATGA T^CATGA 1080 TCATGA BstYI 411 GGATCT [GA]^GATC[CT] 1274 AGATCC DraIII 1337 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 1465 CACGATGTG DrdI 692 GACTCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 966 GACCAAGCAGTC EcoNI 585 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 945 CCTGGGTGAGG HgaI 1038 GACGC GACGC(5/10) 699 GCGTC HinfI 471 GAGTC G^A[ACGT]TC 692 GACTC KpnI 112 GGTACC GGTAC^C 798 GGTACC MflI 411 GGATCT [GA]^GATC[CT] 1274 AGATCC NciI 668 CCCGG CC^[GC]GG 669 CCGGG PleI 692 GACTC GAGTC(4/5) 471 GAGTC PpuMI 581 AGGACCT [GA]G^G[AT]CC[CT] 941 AGGTCCT RcaI 830 TCATGA T^CATGA 1080 TCATGA SfcI 589 CTACAG C^T[GA][CT]AG 1496 CTACAG XhoII 411 GGATCT [GA]^GATC[CT] 1274 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BanI BanII BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeI MboI MboII MnlI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI Van91I