(Circular) (Six-base) MAPSORT of: pFN21AE5692.seq Check: 6954 from: 1 to: 6208 pFN21AE5692 6208 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2465 4018 278 Size: 53 83 186 936 929 1553 2468 Fragments arranged by size: 2468 1553 936 929 186 83 53 AccI GT'mk_AC Cuts at: 1937 2655 3551 1937 Size: 718 896 4594 Fragments arranged by size: 4594 896 718 Acc65I G'GTAC_C Cuts at: 2117 2803 3529 2117 Size: 686 726 4796 Fragments arranged by size: 4796 726 686 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 2348 2765 2921 3122 4271 5655 5923 Size: 563 153 417 156 201 1149 1384 268 Cuts at: 5923 1632 Size: 1917 Fragments arranged by size: 1917 1384 1149 563 417 268 201 156 153 AfeI AGC'GCT Cuts at: 3440 3440 Size: 6208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 5107 6084 1203 Size: 3904 977 1327 Fragments arranged by size: 3904 1327 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 2443 4937 2443 Size: 2494 3714 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3000 1184 Size: 1816 4392 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3113 3145 3113 Size: 32 6176 AlwNI CAG_nnn'CTG Cuts at: 2493 2590 2763 2788 2950 3951 5523 2493 Size: 97 173 25 162 1001 1572 3178 Fragments arranged by size: 3178 1572 1001 173 162 97 25 ApaI G_GGCC'C Cuts at: 2887 2887 Size: 6208 ApaLI G'TGCA_C Cuts at: 3173 4265 5421 3173 Size: 1092 1156 3960 Fragments arranged by size: 3960 1156 1092 ApoI r'AATT_y Cuts at: 1493 2168 3517 3743 3895 6004 1493 Size: 675 1349 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 675 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2673 3533 1870 Size: 73 730 860 4545 Fragments arranged by size: 4545 860 730 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2101 2134 2101 Size: 33 6175 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 2803 2999 3452 3529 Size: 325 217 627 330 686 196 453 77 Cuts at: 3529 4984 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 686 627 453 330 325 217 196 77 BanII G_rGCy'C Cuts at: 1391 2035 2103 2887 3204 3527 1391 Size: 644 68 784 317 323 4072 Fragments arranged by size: 4072 784 644 323 317 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3096 3202 928 Size: 971 1197 106 3934 Fragments arranged by size: 3934 1197 971 106 BbvCI CC'TCA_GC Cuts at: 2809 3053 2809 Size: 244 5964 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4399 4433 1903 Size: 34 2462 34 3678 Fragments arranged by size: 3678 2462 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2048 2333 4100 5310 2048 Size: 285 1767 1210 2946 Fragments arranged by size: 2946 1767 1210 285 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4819 136 Size: 107 122 71 631 605 3147 1525 Fragments arranged by size: 3147 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 3625 3625 Size: 6208 Bme1580I G_kGCm'C Cuts at: 1790 2127 2887 3004 3177 4269 5425 1790 Size: 337 760 117 173 1092 1156 2573 Fragments arranged by size: 2573 1156 1092 760 337 173 117 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3011 3939 4897 Size: 416 53 83 399 24 1594 928 958 Cuts at: 4897 442 Size: 1753 Fragments arranged by size: 1753 1594 958 928 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2494 2684 2987 4868 1239 Size: 1255 190 303 1881 2579 Fragments arranged by size: 2579 1881 1255 303 190 Bpu10I CC'TnA_GC Cuts at: 2552 2809 3053 3198 2552 Size: 257 244 145 5562 Fragments arranged by size: 5562 257 244 145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2258 4333 5198 5496 2258 Size: 2075 865 298 2970 Fragments arranged by size: 2970 2075 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2420 2963 4871 882 Size: 617 921 543 1908 2219 Fragments arranged by size: 2219 1908 921 617 543 BsaAI yAC'GTr Cuts at: 493 1204 3999 493 Size: 711 2795 2702 Fragments arranged by size: 2795 2702 711 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2462 4015 4397 5032 Size: 53 83 186 936 929 1553 382 635 Cuts at: 5032 275 Size: 1451 Fragments arranged by size: 1553 1451 936 929 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2151 4641 5039 5313 5460 5871 2151 Size: 2490 398 274 147 411 2488 Fragments arranged by size: 2490 2488 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2240 2270 3054 3084 3113 Size: 30 165 30 837 30 784 30 29 Cuts at: 3113 3143 1178 Size: 30 4243 Fragments arranged by size: 4243 837 784 165 30 30 30 30 30 29 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3053 3062 1186 Size: 1867 9 4332 Fragments arranged by size: 4332 1867 9 BseYI C'CCAG_C Cuts at: 2026 2767 3441 3464 5411 5907 2026 Size: 741 674 23 1947 496 2327 Fragments arranged by size: 2327 1947 741 674 496 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 3045 1163 Size: 573 498 811 4326 Fragments arranged by size: 4326 811 573 498 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3223 3658 4419 5447 1540 Size: 275 186 1222 435 761 1028 2301 Fragments arranged by size: 2301 1222 1028 761 435 275 186 BsiHKAI G_wGCw'C Cuts at: 2035 3177 4269 4354 5425 2035 Size: 1142 1092 85 1071 2818 Fragments arranged by size: 2818 1142 1092 1071 85 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 BsmBI CGTCTCn'nnnn_ Cuts at: 2711 3035 2711 Size: 324 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2103 2127 2887 3004 3177 3204 Size: 399 245 68 24 760 117 173 27 Cuts at: 3204 3527 4269 4354 5425 1391 Size: 323 742 85 1071 2174 Fragments arranged by size: 2174 1071 760 742 399 323 245 173 117 85 68 27 24 BspEI T'CCGG_A Cuts at: 2151 5871 2151 Size: 3720 2488 BspHI T'CATG_A Cuts at: 2835 3085 4097 5827 2835 Size: 250 1012 1730 3216 Fragments arranged by size: 3216 1730 1012 250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2598 3563 844 Size: 1754 965 3489 Fragments arranged by size: 3489 1754 965 BsrBI CCG'CTC Cuts at: 1394 1823 4095 1394 Size: 429 2272 3507 Fragments arranged by size: 3507 2272 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2046 4703 4877 65 Size: 1183 798 2657 174 1396 Fragments arranged by size: 2657 1396 1183 798 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4852 5039 1700 Size: 110 3042 187 2869 Fragments arranged by size: 3042 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6208 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 2590 794 Size: 1796 4412 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstYI r'GATC_y Cuts at: 1972 2416 3279 3538 3905 4290 4307 5759 5845 Size: 444 863 259 367 385 17 1452 86 Cuts at: 5845 5857 6203 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 863 444 385 367 346 259 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3074 3074 Size: 6208 BtgI C'CryG_G Cuts at: 513 1065 2004 2093 2142 3136 513 Size: 552 939 89 49 994 3585 Fragments arranged by size: 3585 994 939 552 89 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5469 Fragments arranged by size: 5469 683 56 BtsI GCAGTG_nn' Cuts at: 3724 4518 4538 3724 Size: 794 20 5394 Fragments arranged by size: 5394 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DraIII CAC_nnn'GTG Cuts at: 3347 3475 3347 Size: 128 6080 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2703 2977 5215 809 Size: 507 1387 274 2238 1802 Fragments arranged by size: 2238 1802 1387 507 274 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2134 3655 4544 Size: 54 1425 50 275 18 304 1521 889 Cuts at: 4544 6173 8 Size: 1629 43 Fragments arranged by size: 1629 1521 1425 889 304 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3655 1537 Size: 275 1843 4090 Fragments arranged by size: 4090 1843 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4138 1353 Size: 2785 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3122 4795 5169 5315 387 Size: 1064 138 1533 1673 374 146 1280 Fragments arranged by size: 1673 1533 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2033 2033 Size: 6208 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2348 2494 2684 2765 2921 2987 Size: 393 563 153 146 190 81 156 66 Cuts at: 2987 3122 4271 4868 5655 5923 1239 Size: 135 1149 597 787 268 1524 Fragments arranged by size: 1524 1149 787 597 563 393 268 190 156 153 146 135 81 66 EcoNI CCTnn'n_nnAGG Cuts at: 2594 2954 2594 Size: 360 5848 EcoO109I rG'GnC_Cy Cuts at: 2587 2867 2884 2947 2587 Size: 280 17 63 5848 Fragments arranged by size: 5848 280 63 17 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2848 2998 2848 Size: 150 6058 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 3442 5355 1678 Size: 1764 1913 2531 Fragments arranged by size: 2531 1913 1764 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2240 2272 2429 2461 4849 Size: 32 135 32 668 32 157 32 2388 Cuts at: 4849 4881 4923 4955 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2388 668 157 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3430 3552 3798 669 Size: 988 281 1492 122 246 3079 Fragments arranged by size: 3079 1492 988 281 246 122 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KpnI G_GTAC'C Cuts at: 2121 2807 3533 2121 Size: 686 726 4796 Fragments arranged by size: 4796 726 686 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2104 5322 5506 1305 Size: 799 3218 184 2007 Fragments arranged by size: 3218 2007 799 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2136 10 Size: 54 1425 343 304 4082 Fragments arranged by size: 4082 1425 343 304 54 MslI CAynn'nnrTG Cuts at: 518 1166 2199 4166 4525 4684 518 Size: 648 1033 1967 359 159 2042 Fragments arranged by size: 2042 1967 1033 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2543 3624 4301 5449 5694 1606 Size: 937 1081 677 1148 245 2120 Fragments arranged by size: 2120 1148 1081 937 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2093 2142 3136 513 Size: 552 939 89 49 994 3585 Fragments arranged by size: 3585 994 939 552 89 49 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 2779 3566 5111 1132 Size: 97 1550 787 1545 2229 Fragments arranged by size: 2229 1550 1545 787 97 PasI CC'CwG_GG Cuts at: 1348 2854 3300 3326 1348 Size: 1506 446 26 4230 Fragments arranged by size: 4230 1506 446 26 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 2143 2472 2493 2950 3300 1108 Size: 1035 329 21 457 350 4016 Fragments arranged by size: 4016 1035 457 350 329 21 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3113 3145 4277 4309 5790 5822 3113 Size: 32 1132 32 1481 32 3499 Fragments arranged by size: 3499 1481 1132 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2587 2947 2587 Size: 360 5848 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspOMI G'GGCC_C Cuts at: 2883 2883 Size: 6208 PstI C_TGCA'G Cuts at: 830 3560 830 Size: 2730 3478 PvuI CG_AT'CG Cuts at: 2001 3223 2001 Size: 1222 4986 PvuII CAG'CTG Cuts at: 2543 2543 Size: 6208 SacI G_AGCT'C Cuts at: 2035 2035 Size: 6208 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 2231 4456 6168 2231 Size: 2225 1712 2271 Fragments arranged by size: 2271 2225 1712 SfcI C'TryA_G Cuts at: 826 1045 2594 3501 3556 4691 5372 5563 826 Size: 219 1549 907 55 1135 681 191 1471 Fragments arranged by size: 1549 1471 1135 907 681 219 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 2675 3535 1872 Size: 803 860 4545 Fragments arranged by size: 4545 860 803 SmlI C'TyrA_G Cuts at: 820 1017 1943 2273 4312 5213 5475 820 Size: 197 926 330 2039 901 262 1553 Fragments arranged by size: 2039 1553 926 901 330 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6208 StyI C'CwwG_G Cuts at: 513 1065 2004 2093 2142 2645 3136 3647 513 Size: 552 939 89 49 503 491 511 3074 Fragments arranged by size: 3074 939 552 511 503 491 89 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2922 2942 3079 4235 4252 4405 4590 Size: 1255 1342 20 137 1156 17 153 185 Cuts at: 4590 5035 325 Size: 445 1498 Fragments arranged by size: 1498 1342 1255 1156 445 185 153 137 20 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2229 4454 6166 Size: 274 80 33 51 1443 252 2225 1712 Cuts at: 6166 96 Size: 138 Fragments arranged by size: 2225 1712 1443 274 252 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3054 3086 6168 6200 3054 Size: 32 3082 32 3062 Fragments arranged by size: 3082 3062 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3022 3544 3022 Size: 522 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 2673 3533 1870 Size: 803 860 4545 Fragments arranged by size: 4545 860 803 XmnI GAAnn'nnTTC Cuts at: 2916 4337 2916 Size: 1421 4787 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2463 4016 276 Size: 53 83 186 936 929 1553 2468 Fragments arranged by size: 2468 1553 936 929 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbeI BfrBI BplI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI