(Linear) (Six-base) MAPSORT of: KIEE5692.seq Check: 2687 from: 1 to: 1503 KIEE5692.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AatII G_ACGT'C Cuts at: 0 460 1503 Size: 460 1043 AccI GT'mk_AC Cuts at: 0 650 1503 Size: 650 853 Acc65I G'GTAC_C Cuts at: 0 112 798 1503 Size: 112 686 705 Fragments arranged by size: 705 686 112 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 343 760 916 1117 1503 Size: 190 153 417 156 201 386 Fragments arranged by size: 417 386 201 190 156 153 AfeI AGC'GCT Cuts at: 0 1435 1503 Size: 1435 68 AhdI GACnn_n'nnGTC Cuts at: 0 438 1503 Size: 438 1065 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 995 1503 Size: 995 508 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1108 1140 1503 Size: 1108 32 363 Fragments arranged by size: 1108 363 32 AlwNI CAG_nnn'CTG Cuts at: 0 488 585 758 783 945 1503 Size: 488 97 173 25 162 558 Fragments arranged by size: 558 488 173 162 97 25 ApaI G_GGCC'C Cuts at: 0 882 1503 Size: 882 621 ApaLI G'TGCA_C Cuts at: 0 1168 1503 Size: 1168 335 ApoI r'AATT_y Cuts at: 0 163 1503 Size: 163 1340 AvaI C'yCGr_G Cuts at: 0 668 1503 Size: 668 835 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 96 129 1503 Size: 96 33 1374 Fragments arranged by size: 1374 96 33 BanI G'GyrC_C Cuts at: 0 112 798 994 1447 1503 Size: 112 686 196 453 56 Fragments arranged by size: 686 453 196 112 56 BanII G_rGCy'C Cuts at: 0 30 98 882 1199 1503 Size: 30 68 784 317 304 Fragments arranged by size: 784 317 304 68 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 1091 1197 1503 Size: 1091 106 306 Fragments arranged by size: 1091 306 106 BbvCI CC'TCA_GC Cuts at: 0 804 1048 1503 Size: 804 244 455 Fragments arranged by size: 804 455 244 BciVI GTATCCnnnnn_n' Cuts at: 0 43 328 1503 Size: 43 285 1175 Fragments arranged by size: 1175 285 43 Bme1580I G_kGCm'C Cuts at: 0 122 882 999 1172 1503 Size: 122 760 117 173 331 Fragments arranged by size: 760 331 173 122 117 BmrI ACTGGGnnnn_n' Cuts at: 0 1006 1503 Size: 1006 497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 489 679 982 1503 Size: 489 190 303 521 Fragments arranged by size: 521 489 303 190 Bpu10I CC'TnA_GC Cuts at: 0 547 804 1048 1193 1503 Size: 547 257 244 145 310 Fragments arranged by size: 547 310 257 244 145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 1503 Size: 253 1250 BsaI GGTCTCn'nnnn_ Cuts at: 0 415 958 1503 Size: 415 543 545 Fragments arranged by size: 545 543 415 BsaHI Gr'CG_yC Cuts at: 0 457 1503 Size: 457 1046 BsaWI w'CCGG_w Cuts at: 0 146 1503 Size: 146 1357 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 235 265 1049 1079 1108 1138 1503 Size: 235 30 784 30 29 30 365 Fragments arranged by size: 784 365 235 30 30 30 29 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1048 1057 1503 Size: 1048 9 446 Fragments arranged by size: 1048 446 9 BseYI C'CCAG_C Cuts at: 0 21 762 1436 1459 1503 Size: 21 741 674 23 44 Fragments arranged by size: 741 674 44 23 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 1040 1503 Size: 229 811 463 Fragments arranged by size: 811 463 229 BsiEI CG_ry'CG Cuts at: 0 1218 1503 Size: 1218 285 BsiHKAI G_wGCw'C Cuts at: 0 30 1172 1503 Size: 30 1142 331 Fragments arranged by size: 1142 331 30 BsmBI CGTCTCn'nnnn_ Cuts at: 0 706 1030 1503 Size: 706 324 473 Fragments arranged by size: 706 473 324 Bsp1286I G_dGCh'C Cuts at: 0 30 98 122 882 999 1172 1199 1503 Size: 30 68 24 760 117 173 27 304 Fragments arranged by size: 760 304 173 117 68 30 27 24 BspEI T'CCGG_A Cuts at: 0 146 1503 Size: 146 1357 BspHI T'CATG_A Cuts at: 0 830 1080 1503 Size: 830 250 423 Fragments arranged by size: 830 423 250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 593 1503 Size: 593 910 BsrDI GCAATG_nn' Cuts at: 0 41 1503 Size: 41 1462 BstAPI GCAn_nnn'nTGC Cuts at: 0 585 1503 Size: 585 918 BstYI r'GATC_y Cuts at: 0 411 1274 1503 Size: 411 863 229 Fragments arranged by size: 863 411 229 Bsu36I CC'TnA_GG Cuts at: 0 1069 1503 Size: 1069 434 BtgI C'CryG_G Cuts at: 0 88 137 1131 1503 Size: 88 49 994 372 Fragments arranged by size: 994 372 88 49 DraIII CAC_nnn'GTG Cuts at: 0 1342 1470 1503 Size: 1342 128 33 Fragments arranged by size: 1342 128 33 DrdI GACnn_nn'nnGTC Cuts at: 0 698 972 1503 Size: 698 274 531 Fragments arranged by size: 698 531 274 EaeI y'GGCC_r Cuts at: 0 129 1503 Size: 129 1374 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1117 1503 Size: 1117 386 EcoICRI GAG'CTC Cuts at: 0 28 1503 Size: 28 1475 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 343 489 679 760 916 982 1117 Size: 190 153 146 190 81 156 66 135 Cuts at: 1117 1503 Size: 386 Fragments arranged by size: 386 190 190 156 153 146 135 81 66 EcoNI CCTnn'n_nnAGG Cuts at: 0 589 949 1503 Size: 589 360 554 Fragments arranged by size: 589 554 360 EcoO109I rG'GnC_Cy Cuts at: 0 582 862 879 942 1503 Size: 582 280 17 63 561 Fragments arranged by size: 582 561 280 63 17 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 843 993 1503 Size: 843 150 510 Fragments arranged by size: 843 510 150 HaeII r_GCGC'y Cuts at: 0 1437 1503 Size: 1437 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 235 267 424 456 1503 Size: 235 32 157 32 1047 Fragments arranged by size: 1047 235 157 32 32 HincII GTy'rAC Cuts at: 0 1425 1503 Size: 1425 78 KpnI G_GTAC'C Cuts at: 0 116 802 1503 Size: 116 686 701 Fragments arranged by size: 701 686 116 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 99 1503 Size: 99 1404 MscI TGG'CCA Cuts at: 0 131 1503 Size: 131 1372 MslI CAynn'nnrTG Cuts at: 0 194 1503 Size: 194 1309 MspA1I CmG'CkG Cuts at: 0 538 1503 Size: 538 965 NcoI C'CATG_G Cuts at: 0 88 137 1131 1503 Size: 88 49 994 372 Fragments arranged by size: 994 372 88 49 NspI r_CATG'y Cuts at: 0 774 1503 Size: 774 729 PasI CC'CwG_GG Cuts at: 0 849 1295 1321 1503 Size: 849 446 26 182 Fragments arranged by size: 849 446 182 26 PflMI CCAn_nnn'nTGG Cuts at: 0 138 467 488 945 1295 1503 Size: 138 329 21 457 350 208 Fragments arranged by size: 457 350 329 208 138 21 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1108 1140 1503 Size: 1108 32 363 Fragments arranged by size: 1108 363 32 PpuMI rG'GwC_Cy Cuts at: 0 582 942 1503 Size: 582 360 561 Fragments arranged by size: 582 561 360 PspOMI G'GGCC_C Cuts at: 0 878 1503 Size: 878 625 PvuI CG_AT'CG Cuts at: 0 1218 1503 Size: 1218 285 PvuII CAG'CTG Cuts at: 0 538 1503 Size: 538 965 SacI G_AGCT'C Cuts at: 0 30 1503 Size: 30 1473 ScaI AGT'ACT Cuts at: 0 226 1503 Size: 226 1277 SfcI C'TryA_G Cuts at: 0 589 1496 1503 Size: 589 907 7 Fragments arranged by size: 907 589 7 SmaI CCC'GGG Cuts at: 0 670 1503 Size: 670 833 SmlI C'TyrA_G Cuts at: 0 268 1503 Size: 268 1235 StyI C'CwwG_G Cuts at: 0 88 137 640 1131 1503 Size: 88 49 503 491 372 Fragments arranged by size: 503 491 372 88 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 917 937 1074 1503 Size: 917 20 137 429 Fragments arranged by size: 917 429 137 20 TatI w'GTAC_w Cuts at: 0 224 1503 Size: 224 1279 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1049 1081 1503 Size: 1049 32 422 Fragments arranged by size: 1049 422 32 XbaI T'CTAG_A Cuts at: 0 1017 1503 Size: 1017 486 XmaI C'CCGG_G Cuts at: 0 668 1503 Size: 668 835 XmnI GAAnn'nnTTC Cuts at: 0 911 1503 Size: 911 592 ZraI GAC'GTC Cuts at: 0 458 1503 Size: 458 1045 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII BbsI BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstYI Bsu36I BtgI DraIII DrdI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PpiI PpuMI PspOMI PvuI PvuII SacI ScaI SfcI SmaI SmlI StyI TaqII TatI TstI XbaI XmaI XmnI ZraI Enzymes that do not cut: AarI AclI AflII AflIII AgeI AleI AscI AseI AsiSI AvrII BamHI BbeI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsiWI BsmI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI EagI EarI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XcmI XhoI