################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccII AciI AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MluI MluNI MscI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 1160 GTCTAC GT^[AC][GT]AC AccIII 405 TCCGGA T^CCGGA AflII 460 CTTAAG C^TTAAG AflIII 1419 ACATGT A^C[GA][CT]GT AlwNI 955 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 816 ATTAAT AT^TAAT AsnI 816 ATTAAT AT^TAAT Asp700I 1546 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1651 CCCGAG C^[CT]CG[GA]G BanII 270 GGGCTC G[GA]GC[CT]^C BcnI 1623 CCCGG CC^[GC]GG BfrI 460 CTTAAG C^TTAAG BglII 546 AGATCT A^GATCT BmyI 270 GGGCTC G[AGT]GC[ACT]^C BsaWI 405 TCCGGA [AT]^CCGG[AT] BseAI 405 TCCGGA T^CCGGA BsoBI 1651 CCCGAG C^[CT]CG[GA]G Bsp1286I 270 GGGCTC G[AGT]GC[ACT]^C BspEI 405 TCCGGA T^CCGGA BspLU11I 1419 ACATGT A^CATGT BspMI 1616 ACCTGC ACCTGC(4/8) BstAPI 580 GCAACTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cac8I 1627 GCTAGC GC[ACGT]^[ACGT]GC DsaI 381 CCATGG C^C[GA][CT]GG EcoNI 1125 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 977 GAATTC G^AATTC HgaI 120 GCGTC GACGC(5/10) HincII 838 GTTGAC GT[CT]^[GA]AC HindII 838 GTTGAC GT[CT]^[GA]AC HindIII 112 AAGCTT A^AGCTT MaeII 310 ACGT A^CGT MroI 405 TCCGGA T^CCGGA MspA1I 177 CAGCTG C[AC]G^C[GT]G NciI 1623 CCCGG CC^[GC]GG NcoI 381 CCATGG C^CATGG NheI 1627 GCTAGC G^CTAGC NspI 1419 ACATGT [GA]CATG^[CT] PflMI 637 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1049 AGGACCC [GA]G^G[AT]CC[CT] PshBI 816 ATTAAT AT^TAAT PvuII 177 CAGCTG CAG^CTG SapI 1096 GCTCTTC GCTCTTC(1/4) SbfI 346 CCTGCAGG CCTGCA^GG ScaI 668 AGTACT AGT^ACT SmlI 460 CTTAAG C^T[CT][GA]AG Sse8387I 346 CCTGCAGG CCTGCA^GG TaqI 744 TCGA T^CGA TthHB8I 744 TCGA T^CGA Van91I 637 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 940 TCTAGA T^CTAGA XmnI 1546 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BbsI 507 GAAGAC GAAGAC(2/6) 1597 GAAGAC BclI 1025 TGATCA T^GATCA 1229 TGATCA BfaI 941 CTAG C^TAG 1628 CTAG BpmI 96 CTGGAG CTGGAG(16/14) 155 CTGGAG BpuAI 507 GAAGAC GAAGAC(2/6) 1597 GAAGAC Bsp1407I 414 TGTACA T^GTACA 794 TGTACA BsrGI 414 TGTACA T^GTACA 794 TGTACA DraII 464 AGGGCCT [GA]G^G[ACGT]CC[CT] 1049 AGGACCC DraIII 1237 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1366 CACCCTGTG EaeI 1152 TGGCCG [CT]^GGCC[GA] 1203 TGGCCG EcoO109I 464 AGGGCCT [GA]G^G[ACGT]CC[CT] 1049 AGGACCC FbaI 1025 TGATCA T^GATCA 1229 TGATCA HapII 406 CCGG C^CGG 1624 CCGG HpaII 406 CCGG C^CGG 1624 CCGG MaeI 941 CTAG C^TAG 1628 CTAG MfeI 773 CAATTG C^AATTG 806 CAATTG MspI 406 CCGG C^CGG 1624 CCGG MunI 773 CAATTG C^AATTG 806 CAATTG MwoI 411 GCTTGTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 580 GCAACTAATGC PstI 347 CTGCAG CTGCA^G 961 CTGCAG SspBI 414 TGTACA T^GTACA 794 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BbvI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsrDI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII