(Circular) (Six-base) MAPSORT of: pFN21AE5674.seq Check: 2933 from: 1 to: 6373 pFN21AE5674 6373 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4183 278 Size: 53 83 186 936 2647 2468 Fragments arranged by size: 2647 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3166 3716 1937 Size: 1229 550 4594 Fragments arranged by size: 4594 1229 550 Acc65I G'GTAC_C Cuts at: 3694 3694 Size: 6373 AclI AA'CG_TT Cuts at: 1563 4500 4873 1563 Size: 2937 373 3063 Fragments arranged by size: 3063 2937 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2531 2690 4436 5820 6088 1632 Size: 899 159 1746 1384 268 1917 Fragments arranged by size: 1917 1746 1384 899 268 159 AflII C'TTAA_G Cuts at: 820 1017 2465 820 Size: 197 1448 4728 Fragments arranged by size: 4728 1448 197 AflIII A'CryG_T Cuts at: 1203 3424 5272 6249 1203 Size: 2221 1848 977 1327 Fragments arranged by size: 2221 1848 1327 977 AgeI A'CCGG_T Cuts at: 5204 5204 Size: 6373 AhdI GACnn_n'nnGTC Cuts at: 5102 5102 Size: 6373 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6373 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3484 1184 Size: 2300 4073 AlwNI CAG_nnn'CTG Cuts at: 2965 4116 5688 2965 Size: 1151 1572 3650 Fragments arranged by size: 3650 1572 1151 ApaLI G'TGCA_C Cuts at: 4430 5586 4430 Size: 1156 5217 ApoI r'AATT_y Cuts at: 1493 2081 2440 2450 2921 2982 3682 3908 4060 Size: 588 359 10 471 61 700 226 152 Cuts at: 4060 6169 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 700 588 471 359 226 152 61 10 AseI AT'TA_AT Cuts at: 160 1032 2822 4927 160 Size: 872 1790 2105 1606 Fragments arranged by size: 2105 1790 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 AvaI C'yCGr_G Cuts at: 1870 1943 3656 3698 1870 Size: 73 1713 42 4545 Fragments arranged by size: 4545 1713 73 42 BamHI G'GATC_C Cuts at: 3703 4070 3703 Size: 367 6006 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3694 5149 618 Size: 325 217 627 1907 1455 1842 Fragments arranged by size: 1907 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2279 3692 1391 Size: 888 1413 4072 Fragments arranged by size: 4072 1413 888 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2519 3609 928 Size: 971 620 1090 3692 Fragments arranged by size: 3692 1090 971 620 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4564 4598 1903 Size: 34 2627 34 3678 Fragments arranged by size: 3678 2627 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4265 5475 4265 Size: 1210 5163 BclI T'GATC_A Cuts at: 1548 3030 3234 1548 Size: 1482 204 4687 Fragments arranged by size: 4687 1482 204 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4984 136 Size: 107 122 71 631 605 3312 1525 Fragments arranged by size: 3312 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2551 6368 2551 Size: 3817 2556 BlpI GC'TnA_GC Cuts at: 3790 3790 Size: 6373 Bme1580I G_kGCm'C Cuts at: 1790 4434 5590 1790 Size: 2644 1156 2573 Fragments arranged by size: 2644 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2316 1318 Size: 998 5375 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2282 2933 4104 Size: 416 53 83 399 24 865 651 1171 Cuts at: 4104 5062 442 Size: 958 1753 Fragments arranged by size: 1753 1171 958 865 651 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3636 1056 Size: 2580 3793 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2122 2181 3690 5033 1239 Size: 883 59 1509 1343 2579 Fragments arranged by size: 2579 1509 1343 883 59 Bpu10I CC'TnA_GC Cuts at: 2013 2429 2013 Size: 416 5957 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4498 5363 5661 4498 Size: 865 298 5210 Fragments arranged by size: 5210 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5036 882 Size: 617 3537 2219 Fragments arranged by size: 3537 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4164 493 Size: 711 2960 2702 Fragments arranged by size: 2960 2702 711 BsaBI GATnn'nnATC Cuts at: 6367 6367 Size: 6373 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4180 4562 5197 275 Size: 53 83 186 936 2647 382 635 1451 Fragments arranged by size: 2647 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2410 4806 5204 5478 5625 6036 2410 Size: 2396 398 274 147 411 2747 Fragments arranged by size: 2747 2396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2638 2668 2701 2731 1178 Size: 30 165 30 1235 30 33 30 4820 Fragments arranged by size: 4820 1235 165 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2860 3135 1186 Size: 856 818 275 4424 Fragments arranged by size: 4424 856 818 275 BseYI C'CCAG_C Cuts at: 2928 3571 5576 6072 2928 Size: 643 2005 496 3229 Fragments arranged by size: 3229 2005 643 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2360 2959 3431 3454 1163 Size: 573 624 599 472 23 4082 Fragments arranged by size: 4082 624 599 573 472 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3823 4584 5612 1540 Size: 275 186 1822 761 1028 2301 Fragments arranged by size: 2301 1822 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4434 4519 5590 4434 Size: 85 1071 5217 Fragments arranged by size: 5217 1071 85 BsmI GAATG_Cn' Cuts at: 3884 3977 3884 Size: 93 6280 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2279 3692 4434 4519 5590 1391 Size: 399 489 1413 742 85 1071 2174 Fragments arranged by size: 2174 1413 1071 742 489 399 85 BspEI T'CCGG_A Cuts at: 2410 6036 2410 Size: 3626 2747 BspHI T'CATG_A Cuts at: 4262 5992 4262 Size: 1730 4643 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3630 3728 844 Size: 2786 98 3489 Fragments arranged by size: 3489 2786 98 BsrBI CCG'CTC Cuts at: 1394 1823 4260 1394 Size: 429 2437 3507 Fragments arranged by size: 3507 2437 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2195 2238 3483 3585 4868 5042 65 Size: 1183 947 43 1245 102 1283 174 1396 Fragments arranged by size: 1396 1283 1245 1183 947 174 102 43 BsrFI r'CCGG_y Cuts at: 1700 1810 5017 5204 1700 Size: 110 3207 187 2869 Fragments arranged by size: 3207 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2419 2799 96 Size: 2323 380 3670 Fragments arranged by size: 3670 2323 380 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6373 BssSI C'ACGA_G Cuts at: 4433 5445 4433 Size: 1012 5361 BstAPI GCAn_nnn'nTGC Cuts at: 794 2591 794 Size: 1797 4576 BstBI TT'CG_AA Cuts at: 5177 5177 Size: 6373 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6373 BstYI r'GATC_y Cuts at: 1972 2347 2551 3665 3703 4070 4455 4472 5924 Size: 375 204 1114 38 367 385 17 1452 Cuts at: 5924 6010 6022 6368 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1114 385 375 367 346 204 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 2386 513 Size: 552 1321 4500 Fragments arranged by size: 4500 1321 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5634 Fragments arranged by size: 5634 683 56 BtsI GCAGTG_nn' Cuts at: 3118 3889 4683 4703 3118 Size: 771 794 20 4788 Fragments arranged by size: 4788 794 771 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6373 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6338 DraI TTT'AAA Cuts at: 3677 3677 Size: 6373 DraIII CAC_nnn'GTG Cuts at: 3247 3376 3247 Size: 129 6244 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5380 809 Size: 507 4064 1802 Fragments arranged by size: 4064 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3157 3208 3820 Size: 54 1425 50 275 18 1327 51 612 Cuts at: 3820 4709 6338 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1327 889 612 275 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3820 1537 Size: 275 2008 4090 Fragments arranged by size: 4090 2008 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 3108 3153 4303 1353 Size: 782 973 45 1150 3423 Fragments arranged by size: 3423 1150 973 782 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4960 5334 5480 387 Size: 1064 138 3371 374 146 1280 Fragments arranged by size: 3371 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2181 2531 2690 3690 4436 5033 Size: 393 490 59 350 159 1000 746 597 Cuts at: 5033 5820 6088 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1000 787 746 597 490 393 350 268 159 59 EcoNI CCTnn'n_nnAGG Cuts at: 3134 3134 Size: 6373 EcoO109I rG'GnC_Cy Cuts at: 2470 3055 2470 Size: 585 5788 EcoRI G'AATT_C Cuts at: 2982 3682 4060 2982 Size: 700 378 5295 Fragments arranged by size: 5295 700 378 FspI TGC'GCA Cuts at: 4879 4879 Size: 6373 HaeII r_GCGC'y Cuts at: 1678 5520 1678 Size: 3842 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2041 2073 2638 2670 2701 Size: 32 135 32 469 32 565 32 31 Cuts at: 2701 2733 3650 3682 5014 5046 5088 5120 1373 Size: 32 917 32 1332 32 42 32 2626 Fragments arranged by size: 2626 1332 917 565 469 135 42 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2845 3717 3963 669 Size: 988 281 907 872 246 3079 Fragments arranged by size: 3079 988 907 872 281 246 HindIII A'AGCT_T Cuts at: 748 2117 748 Size: 1369 5004 HpaI GTT'AAC Cuts at: 3963 3963 Size: 6373 KpnI G_GTAC'C Cuts at: 3698 3698 Size: 6373 MfeI C'AATT_G Cuts at: 2778 2811 3972 2778 Size: 33 1161 5179 Fragments arranged by size: 5179 1161 33 MluI A'CGCG_T Cuts at: 6249 6249 Size: 6373 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3435 5487 5671 1305 Size: 2130 2052 184 2007 Fragments arranged by size: 2130 2052 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4551 Fragments arranged by size: 4551 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2286 2622 3518 4331 4690 4849 518 Size: 648 1120 336 896 813 359 159 2042 Fragments arranged by size: 2042 1120 896 813 648 359 336 159 MspA1I CmG'CkG Cuts at: 1606 2184 3789 4466 5614 5859 1606 Size: 578 1605 677 1148 245 2120 Fragments arranged by size: 2120 1605 1148 677 578 245 NcoI C'CATG_G Cuts at: 513 1065 2386 513 Size: 552 1321 4500 Fragments arranged by size: 4500 1321 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6373 NheI G'CTAG_C Cuts at: 1052 3632 1052 Size: 2580 3793 NotI GC'GGCC_GC Cuts at: 3820 3820 Size: 6373 NspI r_CATG'y Cuts at: 1132 1229 3428 3731 5276 1132 Size: 97 2199 303 1545 2229 Fragments arranged by size: 2229 2199 1545 303 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6373 PciI A'CATG_T Cuts at: 3424 5272 3424 Size: 1848 4525 PflMI CCAn_nnn'nTGG Cuts at: 1108 2648 1108 Size: 1540 4833 PfoI T'CCnGG_A Cuts at: 2061 2061 Size: 6373 PmeI GTTT'AAAC Cuts at: 3677 3677 Size: 6373 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2940 2972 4442 4474 5955 5987 2940 Size: 32 1470 32 1481 32 3326 Fragments arranged by size: 3326 1481 1470 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3055 3055 Size: 6373 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6373 PsiI TTA'TAA Cuts at: 3943 3943 Size: 6373 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2038 2070 2657 2689 2038 Size: 32 587 32 5722 Fragments arranged by size: 5722 587 32 32 PstI C_TGCA'G Cuts at: 830 2356 2970 3725 830 Size: 1526 614 755 3478 Fragments arranged by size: 3478 1526 755 614 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6373 PvuII CAG'CTG Cuts at: 2184 2184 Size: 6373 SalI G'TCGA_C Cuts at: 1936 3715 1936 Size: 1779 4594 SapI GCTCTTCn'nnn_ Cuts at: 3108 3108 Size: 6373 SbfI CC_TGCA'GG Cuts at: 2356 3725 2356 Size: 1369 5004 ScaI AGT'ACT Cuts at: 2675 4621 6333 2675 Size: 1946 1712 2715 Fragments arranged by size: 2715 1946 1712 SfcI C'TryA_G Cuts at: 826 1045 2352 2966 3167 3721 4856 5537 5728 Size: 219 1307 614 201 554 1135 681 191 Cuts at: 5728 826 Size: 1471 Fragments arranged by size: 1471 1307 1135 681 614 554 219 201 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6373 SmaI CCC'GGG Cuts at: 1872 3700 1872 Size: 1828 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2465 4477 5378 5640 820 Size: 197 926 522 2012 901 262 1553 Fragments arranged by size: 2012 1553 926 901 522 262 197 SnaBI TAC'GTA Cuts at: 493 4164 493 Size: 3671 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6373 SphI G_CATG'C Cuts at: 1132 3731 1132 Size: 2599 3774 SspI AAT'ATT Cuts at: 5 52 4297 5 Size: 47 4245 2081 Fragments arranged by size: 4245 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6373 StyI C'CwwG_G Cuts at: 513 1065 2386 2601 2642 3812 513 Size: 552 1321 215 41 1170 3074 Fragments arranged by size: 3074 1321 1170 552 215 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4400 4417 4570 4755 5200 325 Size: 1255 2820 17 153 185 445 1498 Fragments arranged by size: 2820 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2419 2523 2574 Size: 274 80 33 51 1443 442 104 51 Cuts at: 2574 2673 2799 4619 6331 96 Size: 99 126 1820 1712 138 Fragments arranged by size: 1820 1712 1443 442 274 138 126 104 99 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2968 3000 6333 6365 2968 Size: 32 3333 32 2976 Fragments arranged by size: 3333 2976 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6373 XbaI T'CTAG_A Cuts at: 2945 3709 2945 Size: 764 5609 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6373 XmaI C'CCGG_G Cuts at: 1870 3698 1870 Size: 1828 4545 XmnI GAAnn'nnTTC Cuts at: 3555 4502 3555 Size: 947 5426 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4181 276 Size: 53 83 186 936 2647 2468 Fragments arranged by size: 2647 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI