(Linear) (Six-base) MAPSORT of: KIEE5674.seq Check: 5508 from: 1 to: 1668 KIEE5674.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AccI GT'mk_AC Cuts at: 0 1161 1668 Size: 1161 507 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 526 685 1668 Size: 526 159 983 Fragments arranged by size: 983 526 159 AflII C'TTAA_G Cuts at: 0 460 1668 Size: 460 1208 AflIII A'CryG_T Cuts at: 0 1419 1668 Size: 1419 249 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1479 1668 Size: 1479 189 AlwNI CAG_nnn'CTG Cuts at: 0 960 1668 Size: 960 708 ApoI r'AATT_y Cuts at: 0 76 435 445 916 977 1668 Size: 76 359 10 471 61 691 Fragments arranged by size: 691 471 359 76 61 10 AseI AT'TA_AT Cuts at: 0 817 1668 Size: 817 851 AvaI C'yCGr_G Cuts at: 0 1651 1668 Size: 1651 17 BanII G_rGCy'C Cuts at: 0 274 1668 Size: 274 1394 BbsI GAAGACnn'nnnn_ Cuts at: 0 514 1604 1668 Size: 514 1090 64 Fragments arranged by size: 1090 514 64 BclI T'GATC_A Cuts at: 0 1025 1229 1668 Size: 1025 204 439 Fragments arranged by size: 1025 439 204 BglII A'GATC_T Cuts at: 0 546 1668 Size: 546 1122 BmgBI CAC'GTC Cuts at: 0 311 1668 Size: 311 1357 BmrI ACTGGGnnnn_n' Cuts at: 0 277 928 1668 Size: 277 651 740 Fragments arranged by size: 740 651 277 BmtI G_CTAG'C Cuts at: 0 1631 1668 Size: 1631 37 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 117 176 1668 Size: 117 59 1492 Fragments arranged by size: 1492 117 59 Bpu10I CC'TnA_GC Cuts at: 0 8 424 1668 Size: 8 416 1244 Fragments arranged by size: 1244 416 8 BsaWI w'CCGG_w Cuts at: 0 405 1668 Size: 405 1263 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 633 663 696 726 1668 Size: 633 30 33 30 942 Fragments arranged by size: 942 633 33 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 855 1130 1668 Size: 37 818 275 538 Fragments arranged by size: 818 538 275 37 BseYI C'CCAG_C Cuts at: 0 923 1566 1668 Size: 923 643 102 Fragments arranged by size: 923 643 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 355 954 1426 1449 1668 Size: 355 599 472 23 219 Fragments arranged by size: 599 472 355 219 23 Bsp1286I G_dGCh'C Cuts at: 0 274 1668 Size: 274 1394 BspEI T'CCGG_A Cuts at: 0 405 1668 Size: 405 1263 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1625 1668 Size: 1625 43 BsrDI GCAATG_nn' Cuts at: 0 190 233 1478 1580 1668 Size: 190 43 1245 102 88 Fragments arranged by size: 1245 190 102 88 43 BsrGI T'GTAC_A Cuts at: 0 414 794 1668 Size: 414 380 874 Fragments arranged by size: 874 414 380 BstAPI GCAn_nnn'nTGC Cuts at: 0 586 1668 Size: 586 1082 BstYI r'GATC_y Cuts at: 0 342 546 1660 1668 Size: 342 204 1114 8 Fragments arranged by size: 1114 342 204 8 BtgI C'CryG_G Cuts at: 0 381 1668 Size: 381 1287 BtsI GCAGTG_nn' Cuts at: 0 1113 1668 Size: 1113 555 DraIII CAC_nnn'GTG Cuts at: 0 1242 1371 1668 Size: 1242 129 297 Fragments arranged by size: 1242 297 129 EaeI y'GGCC_r Cuts at: 0 1152 1203 1668 Size: 1152 51 465 Fragments arranged by size: 1152 465 51 EarI CTCTTCn'nnn_ Cuts at: 0 130 1103 1148 1668 Size: 130 973 45 520 Fragments arranged by size: 973 520 130 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 117 176 526 685 1668 Size: 117 59 350 159 983 Fragments arranged by size: 983 350 159 117 59 EcoNI CCTnn'n_nnAGG Cuts at: 0 1129 1668 Size: 1129 539 EcoO109I rG'GnC_Cy Cuts at: 0 465 1050 1668 Size: 465 585 618 Fragments arranged by size: 618 585 465 EcoRI G'AATT_C Cuts at: 0 977 1668 Size: 977 691 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 36 68 633 665 696 728 1645 1668 Size: 36 32 565 32 31 32 917 23 Fragments arranged by size: 917 565 36 32 32 32 31 23 HincII GTy'rAC Cuts at: 0 840 1668 Size: 840 828 HindIII A'AGCT_T Cuts at: 0 112 1668 Size: 112 1556 MfeI C'AATT_G Cuts at: 0 773 806 1668 Size: 773 33 862 Fragments arranged by size: 862 773 33 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1430 1668 Size: 1430 238 MslI CAynn'nnrTG Cuts at: 0 281 617 1513 1668 Size: 281 336 896 155 Fragments arranged by size: 896 336 281 155 MspA1I CmG'CkG Cuts at: 0 179 1668 Size: 179 1489 NcoI C'CATG_G Cuts at: 0 381 1668 Size: 381 1287 NheI G'CTAG_C Cuts at: 0 1627 1668 Size: 1627 41 NspI r_CATG'y Cuts at: 0 1423 1668 Size: 1423 245 PciI A'CATG_T Cuts at: 0 1419 1668 Size: 1419 249 PflMI CCAn_nnn'nTGG Cuts at: 0 643 1668 Size: 643 1025 PfoI T'CCnGG_A Cuts at: 0 56 1668 Size: 56 1612 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 935 967 1668 Size: 935 32 701 Fragments arranged by size: 935 701 32 PpuMI rG'GwC_Cy Cuts at: 0 1050 1668 Size: 1050 618 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 33 65 652 684 1668 Size: 33 32 587 32 984 Fragments arranged by size: 984 587 33 32 32 PstI C_TGCA'G Cuts at: 0 351 965 1668 Size: 351 614 703 Fragments arranged by size: 703 614 351 PvuII CAG'CTG Cuts at: 0 179 1668 Size: 179 1489 SapI GCTCTTCn'nnn_ Cuts at: 0 1103 1668 Size: 1103 565 SbfI CC_TGCA'GG Cuts at: 0 351 1668 Size: 351 1317 ScaI AGT'ACT Cuts at: 0 670 1668 Size: 670 998 SfcI C'TryA_G Cuts at: 0 347 961 1162 1668 Size: 347 614 201 506 Fragments arranged by size: 614 506 347 201 SmlI C'TyrA_G Cuts at: 0 460 1668 Size: 460 1208 StyI C'CwwG_G Cuts at: 0 381 596 637 1668 Size: 381 215 41 1031 Fragments arranged by size: 1031 381 215 41 TatI w'GTAC_w Cuts at: 0 414 518 569 668 794 1668 Size: 414 104 51 99 126 874 Fragments arranged by size: 874 414 126 104 99 51 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 963 995 1668 Size: 963 32 673 Fragments arranged by size: 963 673 32 XbaI T'CTAG_A Cuts at: 0 940 1668 Size: 940 728 XmnI GAAnn'nnTTC Cuts at: 0 1550 1668 Size: 1550 118 Enzymes that do cut: AccI AcuI AflII AflIII AlfI AlwNI ApoI AseI AvaI BanII BbsI BclI BglII BmgBI BmrI BmtI BpmI Bpu10I BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspEI BspMI BsrDI BsrGI BstAPI BstYI BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII MfeI MmeI MslI MspA1I NcoI NheI NspI PciI PflMI PfoI PpiI PpuMI PsrI PstI PvuII SapI SbfI ScaI SfcI SmlI StyI TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BplI BpuEI BsaI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PmeI PmlI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XcmI XhoI XmaI ZraI