################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsiWI BsmBI BsmI Bsp1407I BspHI BspLU11I BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstZ17I CelII CpoI DraI DraIII DrdI Eam1105I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfoI SfuI SgrAI SmlI SpeI Sse8387I SspBI SspI StuI SwaI Tsp509I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 418 TCCGGA T^CCGGA AocI 1572 CCTGAGG CC^T[ACGT]AGG Aor51HI 707 AGCGCT AGC^GCT Asp700I 1242 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1567 GAGCAC G[AT]GC[AT]^C AspI 667 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 289 GGGCTC G[GA]GC[CT]^C BbsI 1024 GAAGAC GAAGAC(2/6) BfaI 448 CTAG C^TAG BglII 476 AGATCT A^GATCT BpmI 1156 CTCCAG CTGGAG(16/14) BpuAI 1024 GAAGAC GAAGAC(2/6) BsaI 337 GAGACC GGTCTC(1/5) BsaWI 418 TCCGGA [AT]^CCGG[AT] BseAI 418 TCCGGA T^CCGGA BsiEI 275 CGGCCG CG[GA][CT]^CG BsiHKAI 1567 GAGCAC G[AT]GC[AT]^C BspDI 403 ATCGAT AT^CGAT BspEI 418 TCCGGA T^CCGGA BsrDI 684 CATTGC GCAATG(2/0) BsrFI 218 GCCGGC [GA]^CCGG[CT] BstEII 579 GGTCACC G^GT[ACGT]ACC BstPI 579 GGTCACC G^GT[ACGT]ACC BstXI 1231 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1572 CCTGAGG CC^T[ACGT]AGG Cfr10I 218 GCCGGC [GA]^CCGG[CT] ClaI 403 ATCGAT AT^CGAT DraII 1234 AGGGCCT [GA]G^G[ACGT]CC[CT] EagI 275 CGGCCG C^GGCCG EclXI 275 CGGCCG C^GGCCG Eco47III 707 AGCGCT AGC^GCT Eco52I 275 CGGCCG C^GGCCG Eco81I 1572 CCTGAGG CC^T[ACGT]AGG EcoO109I 1234 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 579 GGTCACC G^GT[ACGT]ACC HaeII 707 AGCGCT [GA]GCGC^[CT] MaeI 448 CTAG C^TAG MroI 418 TCCGGA T^CCGGA MseI 1298 TTAA T^TAA MslI 870 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 218 GCCGGC GCC^GGC NgoMIV 218 GCCGGC G^CCGGC NspI 208 GCATGC [GA]CATG^[CT] PflFI 667 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ScaI 626 AGTACT AGT^ACT SfiI 276 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 488 CCCGGG CCC^GGG SnaBI 1521 TACGTA TAC^GTA SphI 208 GCATGC GCATG^C TfiI 1587 GAATC G^A[AT]TC Tru9I 1298 TTAA T^TAA Tth111I 667 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 488 CCCGGG C^CCGGG XmaI 488 CCCGGG C^CCGGG XmnI 1242 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 969 GATCC GGATC(4/5) 401 GGATC AvaI 488 CCCGGG C^[CT]CG[GA]G 1335 CCCGAG AvaII 444 GGACC G^G[AT]CC 666 GGACC BalI 701 TGGCCA TGG^CCA 803 TGGCCA BglI 277 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1154 GCCTCCAGGGC BsaAI 619 TACGTG [CT]AC^GT[GA] 1521 TACGTA BseRI 760 CTCCTC GAGGAG(10/8) 1056 CTCCTC BsoBI 488 CCCGGG C^[CT]CG[GA]G 1335 CCCGAG BspMI 500 ACCTGC ACCTGC(4/8) 1028 ACCTGC BssHII 10 GCGCGC G^CGCGC 948 GCGCGC BstYI 476 AGATCT [GA]^GATC[CT] 968 AGATCC EarI 1006 CTCTTC CTCTTC(1/4) 1180 CTCTTC HgaI 1126 GACGC GACGC(5/10) 56 GCGTC Ksp632I 1006 CTCTTC CTCTTC(1/4) 1180 CTCTTC MflI 476 AGATCT [GA]^GATC[CT] 968 AGATCC MluNI 701 TGGCCA TGG^CCA 803 TGGCCA MscI 701 TGGCCA TGG^CCA 803 TGGCCA NcoI 584 CCATGG C^CATGG 1088 CCATGG NlaIV 825 GGCTCC GG[ACGT]^[ACGT]CC 1469 GGAACC PvuII 358 CAGCTG CAG^CTG 1078 CAGCTG SexAI 197 ACCTGGT A^CC[AT]GGT 668 ACCTGGT TaqI 404 TCGA T^CGA 730 TCGA TthHB8I 404 TCGA T^CGA 730 TCGA XcmI 387 CCACACCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 713 CCAAGTGTCTCCTGG XhoII 476 AGATCT [GA]^GATC[CT] 968 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI BbvI BcnI BmyI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI