(Circular) (Six-base) MAPSORT of: pFN21AE5669.seq Check: 7301 from: 1 to: 6307 pFN21AE5669 6307 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4117 278 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3650 1937 Size: 1713 4594 Acc65I G'GTAC_C Cuts at: 3628 3628 Size: 6307 AclI AA'CG_TT Cuts at: 1563 4434 4807 1563 Size: 2871 373 3063 Fragments arranged by size: 3063 2871 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2089 2537 4370 5754 6022 1632 Size: 457 448 1833 1384 268 1917 Fragments arranged by size: 1917 1833 1384 457 448 268 AfeI AGC'GCT Cuts at: 2714 2714 Size: 6307 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6110 AflIII A'CryG_T Cuts at: 1203 5206 6183 1203 Size: 4003 977 1327 Fragments arranged by size: 4003 1327 977 AgeI A'CCGG_T Cuts at: 5138 5138 Size: 6307 AhdI GACnn_n'nnGTC Cuts at: 5036 5036 Size: 6307 AleI CACnn'nnGTG Cuts at: 1166 2879 1166 Size: 1713 4594 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2236 1184 Size: 1052 5255 AlwNI CAG_nnn'CTG Cuts at: 2464 2515 2938 3052 3363 4050 5622 2464 Size: 51 423 114 311 687 1572 3149 Fragments arranged by size: 3149 1572 687 423 311 114 51 ApaLI G'TGCA_C Cuts at: 4364 5520 4364 Size: 1156 5151 ApoI r'AATT_y Cuts at: 1493 3616 3842 3994 6103 1493 Size: 2123 226 152 2109 1697 Fragments arranged by size: 2123 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4861 160 Size: 872 3829 1606 Fragments arranged by size: 3829 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 AvaI C'yCGr_G Cuts at: 1870 1943 2493 3340 3632 1870 Size: 73 550 847 292 4545 Fragments arranged by size: 4545 847 550 292 73 BamHI G'GATC_C Cuts at: 3637 4004 3637 Size: 367 5940 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3628 5083 618 Size: 325 217 627 1841 1455 1842 Fragments arranged by size: 1842 1841 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2298 3626 1391 Size: 907 1328 4072 Fragments arranged by size: 4072 1328 907 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3036 928 Size: 971 1137 4199 Fragments arranged by size: 4199 1137 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4498 4532 1903 Size: 34 50 34 2477 34 3678 Fragments arranged by size: 3678 2477 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4199 5409 4199 Size: 1210 5097 BclI T'GATC_A Cuts at: 1548 1548 Size: 6307 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2288 3165 Size: 107 122 71 631 605 337 279 877 Cuts at: 3165 4918 136 Size: 1753 1525 Fragments arranged by size: 1753 1525 877 631 605 337 279 122 107 71 BglII A'GATC_T Cuts at: 2481 6302 2481 Size: 3821 2486 BlpI GC'TnA_GC Cuts at: 3724 3724 Size: 6307 Bme1580I G_kGCm'C Cuts at: 1790 2755 3009 4368 5524 1790 Size: 965 254 1359 1156 2573 Fragments arranged by size: 2573 1359 1156 965 254 BmgBI CAC'GTC Cuts at: 1318 2071 1318 Size: 753 5554 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4038 4996 442 Size: 416 53 83 399 24 2621 958 1753 Fragments arranged by size: 2621 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3146 4967 1239 Size: 1907 1821 2579 Fragments arranged by size: 2579 1907 1821 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4432 5297 5595 4432 Size: 865 298 5144 Fragments arranged by size: 5144 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2336 4970 882 Size: 617 837 2634 2219 Fragments arranged by size: 2634 2219 837 617 BsaAI yAC'GTr Cuts at: 493 1204 2626 3528 4098 493 Size: 711 1422 902 570 2702 Fragments arranged by size: 2702 1422 902 711 570 BsaBI GATnn'nnATC Cuts at: 6301 6301 Size: 6307 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4114 4496 5131 275 Size: 53 83 186 936 2581 382 635 1451 Fragments arranged by size: 2581 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2423 4740 5138 5412 5559 5970 2423 Size: 2317 398 274 147 411 2760 Fragments arranged by size: 2760 2317 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3517 3547 1178 Size: 30 165 30 2114 30 3938 Fragments arranged by size: 3938 2114 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2756 3052 1186 Size: 1570 296 4441 Fragments arranged by size: 4441 1570 296 BseYI C'CCAG_C Cuts at: 3536 5510 6006 3536 Size: 1974 496 3837 Fragments arranged by size: 3837 1974 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2039 2261 3098 3249 1163 Size: 573 303 222 837 151 4221 Fragments arranged by size: 4221 837 573 303 222 151 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2283 3757 4518 5546 1540 Size: 275 186 282 1474 761 1028 2301 Fragments arranged by size: 2301 1474 1028 761 282 275 186 BsiHKAI G_wGCw'C Cuts at: 3576 4368 4453 5524 3576 Size: 792 85 1071 4359 Fragments arranged by size: 4359 1071 792 85 BsmI GAATG_Cn' Cuts at: 3818 3911 3818 Size: 93 6214 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 2755 3009 3576 3626 4368 4453 Size: 399 508 457 254 567 50 742 85 Cuts at: 4453 5524 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 567 508 457 399 254 85 50 BspEI T'CCGG_A Cuts at: 2423 5970 2423 Size: 3547 2760 BspHI T'CATG_A Cuts at: 4196 5926 4196 Size: 1730 4577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2514 3042 3662 844 Size: 1670 528 620 3489 Fragments arranged by size: 3489 1670 620 528 BsrBI CCG'CTC Cuts at: 1394 1823 4194 1394 Size: 429 2371 3507 Fragments arranged by size: 3507 2371 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2688 4802 4976 65 Size: 1183 1440 2114 174 1396 Fragments arranged by size: 2114 1440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2223 4951 5138 1700 Size: 110 413 2728 187 2869 Fragments arranged by size: 2869 2728 413 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6307 BssHII G'CGCG_C Cuts at: 1925 2015 2953 1925 Size: 90 938 5279 Fragments arranged by size: 5279 938 90 BssSI C'ACGA_G Cuts at: 4367 5379 4367 Size: 1012 5295 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6307 BstBI TT'CG_AA Cuts at: 5111 5111 Size: 6307 BstEII G'GTnAC_C Cuts at: 1184 2584 1184 Size: 1400 4907 BstXI CCAn_nnnn'nTGG Cuts at: 3243 3243 Size: 6307 BstYI r'GATC_y Cuts at: 1972 2481 2973 3637 4004 4389 4406 5858 5944 Size: 509 492 664 367 385 17 1452 86 Cuts at: 5944 5956 6302 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 664 509 492 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3578 3578 Size: 6307 BtgI C'CryG_G Cuts at: 513 1065 2283 2589 3093 513 Size: 552 1218 306 504 3727 Fragments arranged by size: 3727 1218 552 504 306 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2014 487 Size: 683 56 788 4780 Fragments arranged by size: 4780 788 683 56 BtsI GCAGTG_nn' Cuts at: 2218 2811 3018 3382 3823 4617 4637 2218 Size: 593 207 364 441 794 20 3888 Fragments arranged by size: 3888 794 593 441 364 207 20 ClaI AT'CG_AT Cuts at: 1554 2409 1554 Size: 855 5452 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6272 DraI TTT'AAA Cuts at: 3611 3611 Size: 6307 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5314 809 Size: 507 3998 1802 Fragments arranged by size: 3998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2280 2289 Size: 54 1425 50 275 18 327 123 9 Cuts at: 2289 2706 2784 2808 3198 3754 4643 6272 8 Size: 417 78 24 390 556 889 1629 43 Fragments arranged by size: 1629 1425 889 556 417 390 327 275 123 78 54 50 43 24 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2280 3754 1537 Size: 275 468 1474 4090 Fragments arranged by size: 4090 1474 468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3017 3191 4237 1353 Size: 1664 174 1046 3423 Fragments arranged by size: 3423 1664 1046 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3510 3581 4894 5268 5414 387 Size: 1064 138 1921 71 1313 374 146 1280 Fragments arranged by size: 1921 1313 1280 1064 374 146 138 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2089 2537 3146 4370 4967 5754 6022 Size: 393 457 448 609 1224 597 787 268 Cuts at: 6022 1239 Size: 1524 Fragments arranged by size: 1524 1224 787 609 597 457 448 393 268 EcoO109I rG'GnC_Cy Cuts at: 3240 3240 Size: 6307 EcoRI G'AATT_C Cuts at: 3616 3994 3616 Size: 378 5929 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3053 3053 Size: 6307 FspI TGC'GCA Cuts at: 4813 4813 Size: 6307 HaeII r_GCGC'y Cuts at: 1678 2716 5454 1678 Size: 1038 2738 2531 Fragments arranged by size: 2738 2531 1038 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2398 2430 4948 4980 5022 Size: 32 135 32 826 32 2518 32 42 Cuts at: 5022 5054 1373 Size: 32 2626 Fragments arranged by size: 2626 2518 826 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3651 3897 669 Size: 988 281 1713 246 3079 Fragments arranged by size: 3079 1713 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6307 HpaI GTT'AAC Cuts at: 3897 3897 Size: 6307 KpnI G_GTAC'C Cuts at: 3632 3632 Size: 6307 MfeI C'AATT_G Cuts at: 3906 3906 Size: 6307 MluI A'CGCG_T Cuts at: 6183 6183 Size: 6307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2634 3450 5421 5605 1305 Size: 1329 816 1971 184 2007 Fragments arranged by size: 2007 1971 1329 816 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2708 2810 10 Size: 54 1425 343 876 102 3507 Fragments arranged by size: 3507 1425 876 343 102 54 MslI CAynn'nnrTG Cuts at: 518 1166 2879 4265 4624 4783 518 Size: 648 1713 1386 359 159 2042 Fragments arranged by size: 2042 1713 1386 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2365 2789 3085 3723 4400 5548 5793 1606 Size: 759 424 296 638 677 1148 245 2120 Fragments arranged by size: 2120 1148 759 677 638 424 296 245 NaeI GCC'GGC Cuts at: 2225 2225 Size: 6307 NcoI C'CATG_G Cuts at: 513 1065 2589 3093 513 Size: 552 1524 504 3727 Fragments arranged by size: 3727 1524 552 504 NdeI CA'TA_TG Cuts at: 387 387 Size: 6307 NgoMIV G'CCGG_C Cuts at: 2223 2223 Size: 6307 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6307 NotI GC'GGCC_GC Cuts at: 3754 3754 Size: 6307 NspI r_CATG'y Cuts at: 1132 1229 2217 3665 5210 1132 Size: 97 988 1448 1545 2229 Fragments arranged by size: 2229 1545 1448 988 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6307 PciI A'CATG_T Cuts at: 5206 5206 Size: 6307 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6307 PfoI T'CCnGG_A Cuts at: 2667 2667 Size: 6307 PmeI GTTT'AAAC Cuts at: 3611 3611 Size: 6307 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 5889 5921 4376 Size: 32 1481 32 4762 Fragments arranged by size: 4762 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6307 PsiI TTA'TAA Cuts at: 3877 3877 Size: 6307 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3467 3499 3467 Size: 32 6275 PstI C_TGCA'G Cuts at: 830 2088 2511 2997 3025 3368 3405 3659 830 Size: 1258 423 486 28 343 37 254 3478 Fragments arranged by size: 3478 1258 486 423 343 254 37 28 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6307 PvuII CAG'CTG Cuts at: 2365 3085 2365 Size: 720 5587 SalI G'TCGA_C Cuts at: 1936 3649 1936 Size: 1713 4594 SbfI CC_TGCA'GG Cuts at: 3659 3659 Size: 6307 ScaI AGT'ACT Cuts at: 2633 4555 6267 2633 Size: 1922 1712 2673 Fragments arranged by size: 2673 1922 1712 SexAI A'CCwGG_T Cuts at: 2202 2673 2202 Size: 471 5836 SfcI C'TryA_G Cuts at: 826 1045 2084 2507 2635 2993 3021 3364 3370 Size: 219 1039 423 128 358 28 343 6 Cuts at: 3370 3401 3655 4790 5471 5662 826 Size: 31 254 1135 681 191 1471 Fragments arranged by size: 1471 1135 1039 681 423 358 343 254 219 191 128 31 28 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2288 2288 Size: 6307 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6307 SmaI CCC'GGG Cuts at: 1872 2495 3634 1872 Size: 623 1139 4545 Fragments arranged by size: 4545 1139 623 SmlI C'TyrA_G Cuts at: 820 1017 1943 4411 5312 5574 820 Size: 197 926 2468 901 262 1553 Fragments arranged by size: 2468 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3528 4098 493 Size: 3035 570 2702 Fragments arranged by size: 3035 2702 570 SpeI A'CTAG_T Cuts at: 152 152 Size: 6307 SphI G_CATG'C Cuts at: 1132 2217 3665 1132 Size: 1085 1448 3774 Fragments arranged by size: 3774 1448 1085 SspI AAT'ATT Cuts at: 5 52 4231 5 Size: 47 4179 2081 Fragments arranged by size: 4179 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6307 StyI C'CwwG_G Cuts at: 513 1065 2376 2589 3093 3178 3236 3746 513 Size: 552 1311 213 504 85 58 510 3074 Fragments arranged by size: 3074 1311 552 510 504 213 85 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4334 4351 4504 4689 5134 325 Size: 1255 2754 17 153 185 445 1498 Fragments arranged by size: 2754 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2370 2631 3444 Size: 274 80 33 51 1443 393 261 813 Cuts at: 3444 4553 6265 96 Size: 1109 1712 138 Fragments arranged by size: 1712 1443 1109 813 393 274 261 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2556 2588 6267 6299 2556 Size: 32 3679 32 2564 Fragments arranged by size: 3679 2564 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2675 1316 Size: 1359 4948 XbaI T'CTAG_A Cuts at: 3643 3643 Size: 6307 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2399 2725 2399 Size: 326 5981 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6307 XmaI C'CCGG_G Cuts at: 1870 2493 3632 1870 Size: 623 1139 4545 Fragments arranged by size: 4545 1139 623 XmnI GAAnn'nnTTC Cuts at: 3251 4436 3251 Size: 1185 5122 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4115 276 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI