(Linear) (Six-base) MAPSORT of: KIEE5669.seq Check: 4305 from: 1 to: 1602 KIEE5669.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 532 1602 Size: 84 448 1070 Fragments arranged by size: 1070 448 84 AfeI AGC'GCT Cuts at: 0 709 1602 Size: 709 893 AleI CACnn'nnGTG Cuts at: 0 874 1602 Size: 874 728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 231 1602 Size: 231 1371 AlwNI CAG_nnn'CTG Cuts at: 0 459 510 933 1047 1358 1602 Size: 459 51 423 114 311 244 Fragments arranged by size: 459 423 311 244 114 51 AvaI C'yCGr_G Cuts at: 0 488 1335 1602 Size: 488 847 267 Fragments arranged by size: 847 488 267 BanII G_rGCy'C Cuts at: 0 293 1602 Size: 293 1309 BbsI GAAGACnn'nnnn_ Cuts at: 0 1031 1602 Size: 1031 571 BglI GCCn_nnn'nGGC Cuts at: 0 283 1160 1602 Size: 283 877 442 Fragments arranged by size: 877 442 283 BglII A'GATC_T Cuts at: 0 476 1602 Size: 476 1126 Bme1580I G_kGCm'C Cuts at: 0 750 1004 1602 Size: 750 254 598 Fragments arranged by size: 750 598 254 BmgBI CAC'GTC Cuts at: 0 66 1602 Size: 66 1536 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1141 1602 Size: 1141 461 BsaI GGTCTCn'nnnn_ Cuts at: 0 331 1602 Size: 331 1271 BsaAI yAC'GTr Cuts at: 0 621 1523 1602 Size: 621 902 79 Fragments arranged by size: 902 621 79 BsaWI w'CCGG_w Cuts at: 0 418 1602 Size: 418 1184 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1512 1542 1602 Size: 1512 30 60 Fragments arranged by size: 1512 60 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 751 1047 1602 Size: 751 296 555 Fragments arranged by size: 751 555 296 BseYI C'CCAG_C Cuts at: 0 1531 1602 Size: 1531 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 256 1093 1244 1602 Size: 34 222 837 151 358 Fragments arranged by size: 837 358 222 151 34 BsiEI CG_ry'CG Cuts at: 0 278 1602 Size: 278 1324 BsiHKAI G_wGCw'C Cuts at: 0 1571 1602 Size: 1571 31 Bsp1286I G_dGCh'C Cuts at: 0 293 750 1004 1571 1602 Size: 293 457 254 567 31 Fragments arranged by size: 567 457 293 254 31 BspEI T'CCGG_A Cuts at: 0 418 1602 Size: 418 1184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 509 1037 1602 Size: 509 528 565 Fragments arranged by size: 565 528 509 BsrDI GCAATG_nn' Cuts at: 0 683 1602 Size: 683 919 BsrFI r'CCGG_y Cuts at: 0 218 1602 Size: 218 1384 BssHII G'CGCG_C Cuts at: 0 10 948 1602 Size: 10 938 654 Fragments arranged by size: 938 654 10 BstEII G'GTnAC_C Cuts at: 0 579 1602 Size: 579 1023 BstXI CCAn_nnnn'nTGG Cuts at: 0 1238 1602 Size: 1238 364 BstYI r'GATC_y Cuts at: 0 476 968 1602 Size: 476 492 634 Fragments arranged by size: 634 492 476 Bsu36I CC'TnA_GG Cuts at: 0 1573 1602 Size: 1573 29 BtgI C'CryG_G Cuts at: 0 278 584 1088 1602 Size: 278 306 504 514 Fragments arranged by size: 514 504 306 278 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 9 1602 Size: 9 1593 BtsI GCAGTG_nn' Cuts at: 0 213 806 1013 1377 1602 Size: 213 593 207 364 225 Fragments arranged by size: 593 364 225 213 207 ClaI AT'CG_AT Cuts at: 0 404 1602 Size: 404 1198 EaeI y'GGCC_r Cuts at: 0 152 275 284 701 779 803 1193 1602 Size: 152 123 9 417 78 24 390 409 Fragments arranged by size: 417 409 390 152 123 78 24 9 EagI C'GGCC_G Cuts at: 0 275 1602 Size: 275 1327 EarI CTCTTCn'nnn_ Cuts at: 0 1012 1186 1602 Size: 1012 174 416 Fragments arranged by size: 1012 416 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1505 1576 1602 Size: 1505 71 26 Fragments arranged by size: 1505 71 26 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 532 1141 1602 Size: 84 448 609 461 Fragments arranged by size: 609 461 448 84 EcoO109I rG'GnC_Cy Cuts at: 0 1235 1602 Size: 1235 367 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1048 1602 Size: 1048 554 HaeII r_GCGC'y Cuts at: 0 711 1602 Size: 711 891 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 393 425 1602 Size: 393 32 1177 Fragments arranged by size: 1177 393 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 629 1445 1602 Size: 629 816 157 Fragments arranged by size: 816 629 157 MscI TGG'CCA Cuts at: 0 703 805 1602 Size: 703 102 797 Fragments arranged by size: 797 703 102 MslI CAynn'nnrTG Cuts at: 0 874 1602 Size: 874 728 MspA1I CmG'CkG Cuts at: 0 360 784 1080 1602 Size: 360 424 296 522 Fragments arranged by size: 522 424 360 296 NaeI GCC'GGC Cuts at: 0 220 1602 Size: 220 1382 NcoI C'CATG_G Cuts at: 0 584 1088 1602 Size: 584 504 514 Fragments arranged by size: 584 514 504 NgoMIV G'CCGG_C Cuts at: 0 218 1602 Size: 218 1384 NspI r_CATG'y Cuts at: 0 212 1602 Size: 212 1390 PfoI T'CCnGG_A Cuts at: 0 662 1602 Size: 662 940 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1462 1494 1602 Size: 1462 32 108 Fragments arranged by size: 1462 108 32 PstI C_TGCA'G Cuts at: 0 83 506 992 1020 1363 1400 1602 Size: 83 423 486 28 343 37 202 Fragments arranged by size: 486 423 343 202 83 37 28 PvuII CAG'CTG Cuts at: 0 360 1080 1602 Size: 360 720 522 Fragments arranged by size: 720 522 360 ScaI AGT'ACT Cuts at: 0 628 1602 Size: 628 974 SexAI A'CCwGG_T Cuts at: 0 197 668 1602 Size: 197 471 934 Fragments arranged by size: 934 471 197 SfcI C'TryA_G Cuts at: 0 79 502 630 988 1016 1359 1365 1396 Size: 79 423 128 358 28 343 6 31 Cuts at: 1396 1602 Size: 206 Fragments arranged by size: 423 358 343 206 128 79 31 28 6 SfiI GGCCn_nnn'nGGCC Cuts at: 0 283 1602 Size: 283 1319 SmaI CCC'GGG Cuts at: 0 490 1602 Size: 490 1112 SnaBI TAC'GTA Cuts at: 0 1523 1602 Size: 1523 79 SphI G_CATG'C Cuts at: 0 212 1602 Size: 212 1390 StyI C'CwwG_G Cuts at: 0 371 584 1088 1173 1231 1602 Size: 371 213 504 85 58 371 Fragments arranged by size: 504 371 371 213 85 58 TatI w'GTAC_w Cuts at: 0 365 626 1439 1602 Size: 365 261 813 163 Fragments arranged by size: 813 365 261 163 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 551 583 1602 Size: 551 32 1019 Fragments arranged by size: 1019 551 32 Tth111I GACn'n_nGTC Cuts at: 0 670 1602 Size: 670 932 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 394 720 1602 Size: 394 326 882 Fragments arranged by size: 882 394 326 XmaI C'CCGG_G Cuts at: 0 488 1602 Size: 488 1114 XmnI GAAnn'nnTTC Cuts at: 0 1246 1602 Size: 1246 356 Enzymes that do cut: AcuI AfeI AleI AlfI AlwNI AvaI BanII BbsI BglI BglII Bme1580I BmgBI BpmI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BssHII BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EagI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PfoI PsrI PstI PvuII ScaI SexAI SfcI SfiI SmaI SnaBI SphI StyI TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsiWI BsmI BsmBI BspHI BsrBI BsrGI BssSI BstAPI BstBI BstZ17I CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfoI SgfI SgrAI SmlI SpeI SrfI SspI StuI SwaI TaqII XbaI XhoI ZraI