################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvI BcgI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraII DraIII Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI HinP1I HpaI HpaII KasI KpnI KspI MfeI MluI MroI MslI MspI MunI MvaI MwoI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI TfiI TseI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 31 GACGTC GACGT^C AccII 65 CGCG CG^CG AcyI 31 GACGTC G[GA]^CG[CT]C AflIII 933 ACATGT A^C[GA][CT]GT AlwNI 170 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 885 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 31 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1337 CTCGAG C^[CT]CG[GA]G BbvCI 613 GCTGAGG CCTCAGC(-5/-2) BciVI 1077 GGATAC GTATCC(6/5) BglII 1389 AGATCT A^GATCT BsaBI 757 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 31 GACGTC G[GA]^CG[CT]C BseRI 185 GAGGAG GAGGAG(10/8) BsiEI 61 CGGCCG CG[GA][CT]^CG BsiYI 53 CCGAAGAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 53 CCGAAGAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 889 GCATTC GAATGC(1/-1) BsoBI 1337 CTCGAG C^[CT]CG[GA]G BspDI 527 ATCGAT AT^CGAT BspLU11I 933 ACATGT A^CATGT BstF5I 1347 GGATG GGATG(2/0) BstUI 65 CGCG CG^CG BstXI 204 CCAACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 790 GGGCC G^G[ACGT]CC ClaI 527 ATCGAT AT^CGAT DrdI 828 GACAGGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 61 CGGCCG C^GGCCG EarI 1180 GAAGAG CTCTTC(1/4) EclXI 61 CGGCCG C^GGCCG Eco52I 61 CGGCCG C^GGCCG EcoT14I 284 CCATGG C^C[AT][AT]GG Fnu4HI 63 GCCGC GC^[ACGT]GC FokI 1347 GGATG GGATG(9/13) Hin1I 31 GACGTC G[GA]^CG[CT]C HincII 430 GTTGAC GT[CT]^[GA]AC HindII 430 GTTGAC GT[CT]^[GA]AC HindIII 225 AAGCTT A^AGCTT ItaI 63 GCCGC GC^[ACGT]GC Ksp632I 1180 GAAGAG CTCTTC(1/4) MaeII 32 ACGT A^CGT MamI 757 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 611 CAGCTG C[AC]G^C[GT]G MvnI 65 CGCG CG^CG NcoI 284 CCATGG C^CATGG PaeR7I 1337 CTCGAG C^TCGAG PflFI 31 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 611 CAGCTG CAG^CTG Sau96I 790 GGGCC G^G[ACGT]CC SfaNI 694 GATGC GCATC(5/9) SmlI 1337 CTCGAG C^T[CT][GA]AG StyI 284 CCATGG C^C[AT][AT]GG Tth111I 31 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 366 CCATACGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1337 CTCGAG C^TCGAG XmnI 885 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 64 CCGC CCGC(-3/-1) 4 GCGG AcsI 78 GAATTT [GA]^AATT[CT] 859 GAATTT ApoI 78 GAATTT [GA]^AATT[CT] 859 GAATTT AseI 415 ATTAAT AT^TAAT 516 ATTAAT AsnI 415 ATTAAT AT^TAAT 516 ATTAAT BalI 201 TGGCCA TGG^CCA 1350 TGGCCA BbsI 825 GAAGAC GAAGAC(2/6) 1114 GAAGAC BclI 216 TGATCA T^GATCA 303 TGATCA BfaI 346 CTAG C^TAG 782 CTAG BpmI 710 CTCCAG CTGGAG(16/14) 13 CTGGAG BpuAI 825 GAAGAC GAAGAC(2/6) 1114 GAAGAC BsaJI 180 CCGTGG C^C[ACGT][ACGT]GG 284 CCATGG Bsp1407I 47 TGTACA T^GTACA 132 TGTACA BsrGI 47 TGTACA T^GTACA 132 TGTACA DsaI 180 CCGTGG C^C[GA][CT]GG 284 CCATGG EcoRV 457 GATATC GAT^ATC 1228 GATATC FbaI 216 TGATCA T^GATCA 303 TGATCA MaeI 346 CTAG C^TAG 782 CTAG MluNI 201 TGGCCA TGG^CCA 1350 TGGCCA MscI 201 TGGCCA TGG^CCA 1350 TGGCCA NspI 933 ACATGT [GA]CATG^[CT] 1202 ACATGC PshBI 415 ATTAAT AT^TAAT 516 ATTAAT SfcI 167 CTACAG C^T[GA][CT]AG 1233 CTATAG SspBI 47 TGTACA T^GTACA 132 TGTACA SspI 104 AATATT AAT^ATT 774 AATATT XbaI 345 TCTAGA T^CTAGA 781 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AfaI AluI AlwI BsaI BsmAI BsmFI BsrI BstYI Cac8I DdeI DpnII EaeI HaeIII HinfI HphI MaeIII MboI MboII MflI MnlI MseI NdeII NlaIII PleI RsaI Sau3AI TaqI Tru9I Tsp45I Tsp509I TspRI TthHB8I XhoII