(Circular) (Six-base) MAPSORT of: pFN21AE5646.seq Check: 8727 from: 1 to: 6181 pFN21AE5646 6181 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2040 3991 278 Size: 53 83 186 936 504 1951 2468 Fragments arranged by size: 2468 1951 936 504 186 83 53 AccI GT'mk_AC Cuts at: 1937 3524 1937 Size: 1587 4594 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3204 4244 5628 5896 1632 Size: 1572 1040 1384 268 1917 Fragments arranged by size: 1917 1572 1384 1040 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 2938 5080 6057 1203 Size: 1735 2142 977 1327 Fragments arranged by size: 2142 1735 1327 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 4910 4910 Size: 6181 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6181 AlwNI CAG_nnn'CTG Cuts at: 2180 3924 5496 2180 Size: 1744 1572 2865 Fragments arranged by size: 2865 1744 1572 ApaLI G'TGCA_C Cuts at: 4238 5394 4238 Size: 1156 5025 ApoI r'AATT_y Cuts at: 1493 2083 2864 3490 3716 3868 5977 1493 Size: 590 781 626 226 152 2109 1697 Fragments arranged by size: 2109 1697 781 626 590 226 152 AseI AT'TA_AT Cuts at: 160 1032 2421 2522 4735 160 Size: 872 1389 101 2213 1606 Fragments arranged by size: 2213 1606 1389 872 101 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 3342 3506 1870 Size: 73 1399 164 4545 Fragments arranged by size: 4545 1399 164 73 BamHI G'GATC_C Cuts at: 3511 3878 3511 Size: 367 5814 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3502 4957 618 Size: 325 217 627 1715 1455 1842 Fragments arranged by size: 1842 1715 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3500 1391 Size: 2109 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2837 3126 928 Size: 971 938 289 3983 Fragments arranged by size: 3983 971 938 289 BbvCI CC'TCA_GC Cuts at: 2619 2619 Size: 6181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4372 4406 1903 Size: 34 2435 34 3678 Fragments arranged by size: 3678 2435 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3076 4073 5283 3076 Size: 997 1210 3974 Fragments arranged by size: 3974 1210 997 BclI T'GATC_A Cuts at: 1548 2221 2308 1548 Size: 673 87 5421 Fragments arranged by size: 5421 673 87 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4792 136 Size: 107 122 71 631 605 3120 1525 Fragments arranged by size: 3120 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3394 6176 3394 Size: 2782 3399 BlpI GC'TnA_GC Cuts at: 3598 3598 Size: 6181 Bme1580I G_kGCm'C Cuts at: 1790 4242 5398 1790 Size: 2452 1156 2573 Fragments arranged by size: 2573 2452 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2744 3912 4870 Size: 416 53 83 399 24 1327 1168 958 Cuts at: 4870 442 Size: 1753 Fragments arranged by size: 1753 1327 1168 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2861 2861 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2700 4841 1239 Size: 800 661 2141 2579 Fragments arranged by size: 2579 2141 800 661 Bpu10I CC'TnA_GC Cuts at: 2619 2619 Size: 6181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4306 5171 5469 4306 Size: 865 298 5018 Fragments arranged by size: 5018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2015 2420 2682 3303 3420 4844 882 Size: 617 516 405 262 621 117 1424 2219 Fragments arranged by size: 2219 1424 621 617 516 405 262 117 BsaAI yAC'GTr Cuts at: 493 1204 3972 493 Size: 711 2768 2702 Fragments arranged by size: 2768 2702 711 BsaBI GATnn'nnATC Cuts at: 2766 6175 2766 Size: 3409 2772 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 3988 4370 5005 Size: 53 83 186 936 504 1951 382 635 Cuts at: 5005 275 Size: 1451 Fragments arranged by size: 1951 1451 936 635 504 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4614 5012 5286 5433 5844 4614 Size: 398 274 147 411 4951 Fragments arranged by size: 4951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5956 Fragments arranged by size: 5956 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2205 1186 Size: 1019 5162 BseYI C'CCAG_C Cuts at: 5384 5880 5384 Size: 496 5685 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5608 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2069 3631 4392 5420 1540 Size: 275 186 68 1562 761 1028 2301 Fragments arranged by size: 2301 1562 1028 761 275 186 68 BsiHKAI G_wGCw'C Cuts at: 4242 4327 5398 4242 Size: 85 1071 5025 Fragments arranged by size: 5025 1071 85 BsmI GAATG_Cn' Cuts at: 2894 3692 3785 2894 Size: 798 93 5290 Fragments arranged by size: 5290 798 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3500 4242 4327 5398 1391 Size: 399 1710 742 85 1071 2174 Fragments arranged by size: 2174 1710 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 4070 5800 4070 Size: 1730 4451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3536 844 Size: 2692 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4068 1394 Size: 429 2245 3507 Fragments arranged by size: 3507 2245 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4676 4850 65 Size: 1183 3428 174 1396 Fragments arranged by size: 3428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4825 5012 1700 Size: 110 3015 187 2869 Fragments arranged by size: 3015 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2052 2137 96 Size: 1956 85 4140 Fragments arranged by size: 4140 1956 85 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 4241 5253 4241 Size: 1012 5169 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6181 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6181 BstXI CCAn_nnnn'nTGG Cuts at: 2216 2216 Size: 6181 BstYI r'GATC_y Cuts at: 1972 2318 2767 3394 3511 3878 4263 4280 5732 Size: 346 449 627 117 367 385 17 1452 Cuts at: 5732 5818 5830 6176 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 627 449 385 367 346 346 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2185 2289 513 Size: 552 939 181 104 4405 Fragments arranged by size: 4405 939 552 181 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5442 Fragments arranged by size: 5442 683 56 BtsI GCAGTG_nn' Cuts at: 2311 3697 4491 4511 2311 Size: 1386 794 20 3981 Fragments arranged by size: 3981 1386 794 20 ClaI AT'CG_AT Cuts at: 1554 2533 1554 Size: 979 5202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3030 3065 529 Size: 35 2466 35 3645 Fragments arranged by size: 3645 2466 35 35 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2839 5188 809 Size: 507 1523 2349 1802 Fragments arranged by size: 2349 1802 1523 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2066 2182 2206 Size: 54 1425 50 275 18 236 116 24 Cuts at: 2206 3355 3628 4517 6146 8 Size: 1149 273 889 1629 43 Fragments arranged by size: 1629 1425 1149 889 275 273 236 116 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2066 3628 1537 Size: 275 254 1562 4090 Fragments arranged by size: 4090 1562 275 254 EarI CTCTTCn'nnn_ Cuts at: 1353 3180 4111 1353 Size: 1827 931 3423 Fragments arranged by size: 3423 1827 931 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4768 5142 5288 387 Size: 1064 138 3179 374 146 1280 Fragments arranged by size: 3179 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2700 3204 4244 4841 5628 5896 Size: 393 407 661 504 1040 597 787 268 Cuts at: 5896 1239 Size: 1524 Fragments arranged by size: 1524 1040 787 661 597 504 407 393 268 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 EcoRV GAT'ATC Cuts at: 2464 3235 2464 Size: 771 5410 FspI TGC'GCA Cuts at: 4687 4687 Size: 6181 HaeII r_GCGC'y Cuts at: 1678 5328 1678 Size: 3650 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2184 2216 3113 3145 4822 Size: 32 135 32 612 32 897 32 1677 Cuts at: 4822 4854 4896 4928 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1677 897 612 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2437 3525 3771 669 Size: 988 281 499 1088 246 3079 Fragments arranged by size: 3079 1088 988 499 281 246 HindIII A'AGCT_T Cuts at: 748 2230 748 Size: 1482 4699 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5295 5479 1305 Size: 3990 184 2007 Fragments arranged by size: 3990 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2208 3357 10 Size: 54 1425 343 376 1149 2834 Fragments arranged by size: 2834 1425 1149 376 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4139 4498 4657 518 Size: 648 2973 359 159 2042 Fragments arranged by size: 2973 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2618 3597 4274 5422 5667 1606 Size: 1012 979 677 1148 245 2120 Fragments arranged by size: 2120 1148 1012 979 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2289 513 Size: 552 939 285 4405 Fragments arranged by size: 4405 939 552 285 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 2942 3211 3539 5084 1132 Size: 97 1713 269 328 1545 2229 Fragments arranged by size: 2229 1713 1545 328 269 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6181 PciI A'CATG_T Cuts at: 2938 5080 2938 Size: 2142 4039 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6181 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4250 4282 5763 5795 4250 Size: 32 1481 32 4636 Fragments arranged by size: 4636 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PstI C_TGCA'G Cuts at: 830 3533 830 Size: 2703 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2618 2618 Size: 6181 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SbfI CC_TGCA'GG Cuts at: 3533 3533 Size: 6181 ScaI AGT'ACT Cuts at: 4429 6141 4429 Size: 1712 4469 SfcI C'TryA_G Cuts at: 826 1045 2172 3238 3529 4664 5345 5536 826 Size: 219 1127 1066 291 1135 681 191 1471 Fragments arranged by size: 1471 1135 1127 1066 681 291 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 3508 1872 Size: 1636 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3342 4285 5186 5448 820 Size: 197 926 1399 943 901 262 1553 Fragments arranged by size: 1553 1399 943 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 3539 1132 Size: 2407 3774 SspI AAT'ATT Cuts at: 5 52 2111 2781 4105 5 Size: 47 2059 670 1324 2081 Fragments arranged by size: 2081 2059 1324 670 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6181 StyI C'CwwG_G Cuts at: 513 1065 2004 2289 3620 513 Size: 552 939 285 1331 3074 Fragments arranged by size: 3074 1331 939 552 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3327 3444 4208 4225 4378 4563 5008 Size: 1255 1747 117 764 17 153 185 445 Cuts at: 5008 325 Size: 1498 Fragments arranged by size: 1747 1498 1255 764 445 185 153 117 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 2137 2725 Size: 274 80 33 51 1443 75 85 588 Cuts at: 2725 4427 6139 96 Size: 1702 1712 138 Fragments arranged by size: 1712 1702 1443 588 274 138 85 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2170 2202 6141 6173 2170 Size: 32 3939 32 2178 Fragments arranged by size: 3939 2178 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2039 1316 Size: 723 5458 XbaI T'CTAG_A Cuts at: 2350 2786 3517 2350 Size: 436 731 5014 Fragments arranged by size: 5014 731 436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2378 2378 Size: 6181 XhoI C'TCGA_G Cuts at: 1943 3342 1943 Size: 1399 4782 XmaI C'CCGG_G Cuts at: 1870 3506 1870 Size: 1636 4545 XmnI GAAnn'nnTTC Cuts at: 2894 4310 2894 Size: 1416 4765 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2038 3989 276 Size: 53 83 186 936 504 1951 2468 Fragments arranged by size: 2468 1951 936 504 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI