################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AvaI AviII BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HapII HpaII KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 616 GCGG CCGC(-3/-1) AcyI 1092 GACGCC G[GA]^CG[CT]C AflIII 312 ACGTGT A^C[GA][CT]GT Alw44I 193 GTGCAC G^TGCAC AlwNI 55 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 995 GGGCCC GGGCC^C ApaLI 193 GTGCAC G^TGCAC Asp700I 572 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 193 GTGCAC G[AT]GC[AT]^C AspI 532 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1566 CCTAGG C^CTAGG BalI 668 TGGCCA TGG^CCA BamHI 1441 GGATCC G^GATCC BciVI 181 GTATCC GTATCC(6/5) BglI 997 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1566 CCTAGG C^CTAGG BsaHI 1092 GACGCC G[GA]^CG[CT]C BsgI 551 CTGCAC GTGCAG(16/14) BsiHKAI 193 GTGCAC G[AT]GC[AT]^C BsmFI 1083 GTCCC GGGAC(10/14) Bsp1407I 140 TGTACA T^GTACA BspHI 1217 TCATGA T^CATGA BspMI 266 ACCTGC ACCTGC(4/8) BsrGI 140 TGTACA T^GTACA BstAPI 42 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 449 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 668 TGGCCA [CT]^GGCC[GA] EcoRV 1291 GATATC GAT^ATC HaeII 411 GGCGCT [GA]GCGC^[CT] HgaI 1092 GACGC GACGC(5/10) Hin1I 1092 GACGCC G[GA]^CG[CT]C HindIII 303 AAGCTT A^AGCTT MluNI 668 TGGCCA TGG^CCA MscI 668 TGGCCA TGG^CCA MslI 576 CACTTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 20 CCATGG C^CATGG PflFI 532 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1081 AGGTCCC [GA]G^G[AT]CC[CT] RcaI 1217 TCATGA T^CATGA SapI 787 GAAGAGC GCTCTTC(1/4) SfcI 162 CTGTAG C^T[GA][CT]AG SspBI 140 TGTACA T^GTACA SspI 1327 AATATT AAT^ATT StuI 105 AGGCCT AGG^CCT Tth111I 532 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 904 TCTAGA T^CTAGA XmnI 572 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 168 AAATTT [GA]^AATT[CT] 1495 GAATTT AfaI 141 GTAC GT^AC 1100 GTAC ApoI 168 AAATTT [GA]^AATT[CT] 1495 GAATTT AvaII 1082 GGTCC G^G[AT]CC 1227 GGTCC BpmI 767 CTCCAG CTGGAG(16/14) 214 CTGGAG BseRI 646 CTCCTC GAGGAG(10/8) 1039 GAGGAG BsmI 612 GAATGC GAATGC(1/-1) 1457 GCATTC BstYI 503 AGATCC [GA]^GATC[CT] 1441 GGATCC CfoI 412 GCGC GCG^C 457 GCGC DraII 994 AGGGCCC [GA]G^G[ACGT]CC[CT] 1081 AGGTCCC DsaI 20 CCATGG C^C[GA][CT]GG 980 CCACGG EcoO109I 994 AGGGCCC [GA]G^G[ACGT]CC[CT] 1081 AGGTCCC HhaI 412 GCGC GCG^C 457 GCGC HinP1I 412 GCGC G^CGC 457 GCGC HpaI 597 GTTAAC GTT^AAC 1482 GTTAAC MaeII 312 ACGT A^CGT 1548 ACGT MflI 503 AGATCC [GA]^GATC[CT] 1441 GGATCC PflMI 54 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1161 CCAAGTGGTGG RsaI 141 GTAC GT^AC 1100 GTAC SmlI 713 CTCAAG C^T[CT][GA]AG 1618 CTCAAG TfiI 148 GAATC G^A[AT]TC 914 GAATC Van91I 54 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1161 CCAAGTGGTGG XhoII 503 AGATCC [GA]^GATC[CT] 1441 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AluI AlwI BanII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I