(Circular) (Six-base) MAPSORT of: pFN21AE5638.seq Check: 6523 from: 1 to: 6346 pFN21AE5638 6346 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4156 278 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3689 1937 Size: 1752 4594 Acc65I G'GTAC_C Cuts at: 3667 3667 Size: 6346 AclI AA'CG_TT Cuts at: 1563 4473 4846 1563 Size: 2910 373 3063 Fragments arranged by size: 3063 2910 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2568 2805 3048 3605 4409 5793 6061 1632 Size: 936 237 243 557 804 1384 268 1917 Fragments arranged by size: 1917 1384 936 804 557 268 243 237 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6149 AflIII A'CryG_T Cuts at: 1203 2317 5245 6222 1203 Size: 1114 2928 977 1327 Fragments arranged by size: 2928 1327 1114 977 AgeI A'CCGG_T Cuts at: 5177 5177 Size: 6346 AhdI GACnn_n'nnGTC Cuts at: 5075 5075 Size: 6346 AleI CACnn'nnGTG Cuts at: 1166 2585 1166 Size: 1419 4927 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6346 AlwNI CAG_nnn'CTG Cuts at: 2065 4089 5661 2065 Size: 2024 1572 2750 Fragments arranged by size: 2750 2024 1572 ApaI G_GGCC'C Cuts at: 3004 3004 Size: 6346 ApaLI G'TGCA_C Cuts at: 2198 4403 5559 2198 Size: 2205 1156 2985 Fragments arranged by size: 2985 2205 1156 ApoI r'AATT_y Cuts at: 1493 2173 3500 3655 3881 4033 6142 1493 Size: 680 1327 155 226 152 2109 1697 Fragments arranged by size: 2109 1697 1327 680 226 155 152 AseI AT'TA_AT Cuts at: 160 1032 4900 160 Size: 872 3868 1606 Fragments arranged by size: 3868 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 AvaI C'yCGr_G Cuts at: 1870 1943 3671 1870 Size: 73 1728 4545 Fragments arranged by size: 4545 1728 73 AvrII C'CTAG_G Cuts at: 3571 3571 Size: 6346 BamHI G'GATC_C Cuts at: 3446 3676 4043 3446 Size: 230 367 5749 Fragments arranged by size: 5749 367 230 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3667 5122 618 Size: 325 217 627 1880 1455 1842 Fragments arranged by size: 1880 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2747 2799 3004 3665 1391 Size: 1356 52 205 661 4072 Fragments arranged by size: 4072 1356 661 205 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2634 2718 3101 928 Size: 971 735 84 383 4173 Fragments arranged by size: 4173 971 735 383 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4537 4571 1903 Size: 34 2600 34 3678 Fragments arranged by size: 3678 2600 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2197 4238 5448 2197 Size: 2041 1210 3095 Fragments arranged by size: 3095 2041 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6346 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3008 4957 136 Size: 107 122 71 631 605 1336 1949 1525 Fragments arranged by size: 1949 1525 1336 631 605 122 107 71 BglII A'GATC_T Cuts at: 6341 6341 Size: 6346 BlpI GC'TnA_GC Cuts at: 3763 3763 Size: 6346 Bme1580I G_kGCm'C Cuts at: 1790 2202 3004 3028 3259 4407 5563 1790 Size: 412 802 24 231 1148 1156 2573 Fragments arranged by size: 2573 1156 1148 802 412 231 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6346 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4077 5035 442 Size: 416 53 83 399 24 2660 958 1753 Fragments arranged by size: 2660 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6346 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 2757 5006 1239 Size: 1001 517 2249 2579 Fragments arranged by size: 2579 2249 1001 517 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2703 3608 4471 5336 5634 2703 Size: 905 863 865 298 3415 Fragments arranged by size: 3415 905 865 863 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5009 882 Size: 617 3510 2219 Fragments arranged by size: 3510 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4137 493 Size: 711 2933 2702 Fragments arranged by size: 2933 2702 711 BsaBI GATnn'nnATC Cuts at: 6340 6340 Size: 6346 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3098 4153 4535 5170 Size: 53 83 186 936 1565 1055 382 635 Cuts at: 5170 275 Size: 1451 Fragments arranged by size: 1565 1451 1055 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4779 5177 5451 5598 6009 4779 Size: 398 274 147 411 5116 Fragments arranged by size: 5116 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6121 Fragments arranged by size: 6121 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2642 3059 1186 Size: 1456 417 4473 Fragments arranged by size: 4473 1456 417 BseYI C'CCAG_C Cuts at: 2065 2493 2525 2645 5549 6045 2065 Size: 428 32 120 2904 496 2366 Fragments arranged by size: 2904 2366 496 428 120 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2541 1163 Size: 573 805 4968 Fragments arranged by size: 4968 805 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3796 4557 5585 1540 Size: 275 186 1795 761 1028 2301 Fragments arranged by size: 2301 1795 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2202 4407 4492 5563 2202 Size: 2205 85 1071 2985 Fragments arranged by size: 2985 2205 1071 85 BsmI GAATG_Cn' Cuts at: 2623 3462 3857 3950 2623 Size: 839 395 93 5019 Fragments arranged by size: 5019 839 395 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2747 2799 3004 3028 3259 3665 Size: 399 412 545 52 205 24 231 406 Cuts at: 3665 4407 4492 5563 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 545 412 406 399 231 205 85 52 24 BspEI T'CCGG_A Cuts at: 6009 6009 Size: 6346 BspHI T'CATG_A Cuts at: 3222 4235 5965 3222 Size: 1013 1730 3603 Fragments arranged by size: 3603 1730 1013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 3701 844 Size: 1436 1421 3489 Fragments arranged by size: 3489 1436 1421 BsrBI CCG'CTC Cuts at: 1394 1823 4233 1394 Size: 429 2410 3507 Fragments arranged by size: 3507 2410 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4841 5015 65 Size: 1183 3593 174 1396 Fragments arranged by size: 3593 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4990 5177 1700 Size: 110 3180 187 2869 Fragments arranged by size: 3180 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2145 96 Size: 2049 4297 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6346 BssSI C'ACGA_G Cuts at: 4406 5418 4406 Size: 1012 5334 BstAPI GCAn_nnn'nTGC Cuts at: 794 2053 794 Size: 1259 5087 BstBI TT'CG_AA Cuts at: 5150 5150 Size: 6346 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6346 BstYI r'GATC_y Cuts at: 1972 2508 3446 3676 4043 4428 4445 5897 5983 Size: 536 938 230 367 385 17 1452 86 Cuts at: 5983 5995 6341 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 938 536 385 367 346 230 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2025 2985 513 Size: 552 960 960 3874 Fragments arranged by size: 3874 960 960 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2517 487 Size: 683 56 1291 4316 Fragments arranged by size: 4316 1291 683 56 BtsI GCAGTG_nn' Cuts at: 3862 4656 4676 3862 Size: 794 20 5532 Fragments arranged by size: 5532 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6346 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6311 DraI TTT'AAA Cuts at: 3650 3650 Size: 6346 DraIII CAC_nnn'GTG Cuts at: 2459 2459 Size: 6346 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5353 809 Size: 507 4037 1802 Fragments arranged by size: 4037 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2673 3793 4682 Size: 54 1425 50 275 18 843 1120 889 Cuts at: 4682 6311 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1120 889 843 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3793 1537 Size: 275 1981 4090 Fragments arranged by size: 4090 1981 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2025 2247 2631 2787 3337 3581 4276 1353 Size: 672 222 384 156 550 244 695 3423 Fragments arranged by size: 3423 695 672 550 384 244 222 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4933 5307 5453 387 Size: 1064 138 3344 374 146 1280 Fragments arranged by size: 3344 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2568 2757 2805 3048 3605 4409 Size: 393 608 328 189 48 243 557 804 Cuts at: 4409 5006 5793 6061 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 804 787 608 597 557 393 328 268 243 189 48 EcoO109I rG'GnC_Cy Cuts at: 3000 3087 3000 Size: 87 6259 EcoRI G'AATT_C Cuts at: 3655 4033 3655 Size: 378 5968 EcoRV GAT'ATC Cuts at: 3298 3298 Size: 6346 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2054 2598 2054 Size: 544 5802 FspI TGC'GCA Cuts at: 4852 4852 Size: 6346 HaeII r_GCGC'y Cuts at: 1678 2420 5493 1678 Size: 742 3073 2531 Fragments arranged by size: 3073 2531 742 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2904 2936 3585 3617 4987 Size: 32 135 32 1332 32 649 32 1370 Cuts at: 4987 5019 5061 5093 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1370 1332 649 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2604 2862 3489 3690 3936 669 Size: 988 281 666 258 627 201 246 3079 Fragments arranged by size: 3079 988 666 627 281 258 246 201 HindIII A'AGCT_T Cuts at: 748 2308 748 Size: 1560 4786 HpaI GTT'AAC Cuts at: 2604 3489 3936 2604 Size: 885 447 5014 Fragments arranged by size: 5014 885 447 KpnI G_GTAC'C Cuts at: 3671 3671 Size: 6346 MfeI C'AATT_G Cuts at: 3945 3945 Size: 6346 MluI A'CGCG_T Cuts at: 6222 6222 Size: 6346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5460 5644 1305 Size: 4155 184 2007 Fragments arranged by size: 4155 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 10 Size: 54 1425 343 843 3681 Fragments arranged by size: 3681 1425 843 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 4304 4663 4822 518 Size: 648 1419 1719 359 159 2042 Fragments arranged by size: 2042 1719 1419 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3762 4439 5587 5832 1606 Size: 2156 677 1148 245 2120 Fragments arranged by size: 2156 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2025 513 Size: 552 960 4834 Fragments arranged by size: 4834 960 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6346 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6346 NotI GC'GGCC_GC Cuts at: 3793 3793 Size: 6346 NspI r_CATG'y Cuts at: 1132 1229 3704 5249 1132 Size: 97 2475 1545 2229 Fragments arranged by size: 2475 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6346 PciI A'CATG_T Cuts at: 5245 5245 Size: 6346 PflMI CCAn_nnn'nTGG Cuts at: 1108 2065 3172 1108 Size: 957 1107 4282 Fragments arranged by size: 4282 1107 957 PfoI T'CCnGG_A Cuts at: 2565 2565 Size: 6346 PmeI GTTT'AAAC Cuts at: 3650 3650 Size: 6346 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4415 4447 5928 5960 4415 Size: 32 1481 32 4801 Fragments arranged by size: 4801 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3087 3087 Size: 6346 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6346 PsiI TTA'TAA Cuts at: 3916 3916 Size: 6346 PspOMI G'GGCC_C Cuts at: 3000 3000 Size: 6346 PstI C_TGCA'G Cuts at: 830 3698 830 Size: 2868 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6346 SalI G'TCGA_C Cuts at: 1936 3688 1936 Size: 1752 4594 SapI GCTCTTCn'nnn_ Cuts at: 2787 2787 Size: 6346 SbfI CC_TGCA'GG Cuts at: 3698 3698 Size: 6346 ScaI AGT'ACT Cuts at: 4594 6306 4594 Size: 1712 4634 SfcI C'TryA_G Cuts at: 826 1045 2167 3694 4829 5510 5701 826 Size: 219 1122 1527 1135 681 191 1471 Fragments arranged by size: 1527 1471 1135 1122 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6346 SmaI CCC'GGG Cuts at: 1872 3673 1872 Size: 1801 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2718 3623 4450 5351 5613 820 Size: 197 926 775 905 827 901 262 1553 Fragments arranged by size: 1553 926 905 901 827 775 262 197 SnaBI TAC'GTA Cuts at: 493 4137 493 Size: 3644 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6346 SphI G_CATG'C Cuts at: 1132 3704 1132 Size: 2572 3774 SspI AAT'ATT Cuts at: 5 52 3334 4270 5 Size: 47 3282 936 2081 Fragments arranged by size: 3282 2081 936 47 StuI AGG'CCT Cuts at: 1517 2112 1517 Size: 595 5751 StyI C'CwwG_G Cuts at: 513 1065 2025 2739 3571 3785 513 Size: 552 960 714 832 214 3074 Fragments arranged by size: 3074 960 832 714 552 214 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2228 4373 4390 4543 4728 5173 325 Size: 1255 648 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 648 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 3104 4592 Size: 274 80 33 51 1443 168 959 1488 Cuts at: 4592 6304 96 Size: 1712 138 Fragments arranged by size: 1712 1488 1443 959 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3438 3470 6306 6338 3438 Size: 32 2836 32 3446 Fragments arranged by size: 3446 2836 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2540 1316 Size: 1224 5122 XbaI T'CTAG_A Cuts at: 2909 3682 2909 Size: 773 5573 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6346 XmaI C'CCGG_G Cuts at: 1870 3671 1870 Size: 1801 4545 XmnI GAAnn'nnTTC Cuts at: 2581 4475 2581 Size: 1894 4452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4154 276 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI