(Linear) (Six-base) MAPSORT of: KIEE5638.seq Check: 6881 from: 1 to: 1641 KIEE5638.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 563 800 1043 1600 1641 Size: 563 237 243 557 41 Fragments arranged by size: 563 557 243 237 41 AflIII A'CryG_T Cuts at: 0 312 1641 Size: 312 1329 AleI CACnn'nnGTG Cuts at: 0 580 1641 Size: 580 1061 AlwNI CAG_nnn'CTG Cuts at: 0 60 1641 Size: 60 1581 ApaI G_GGCC'C Cuts at: 0 999 1641 Size: 999 642 ApaLI G'TGCA_C Cuts at: 0 193 1641 Size: 193 1448 ApoI r'AATT_y Cuts at: 0 168 1495 1641 Size: 168 1327 146 Fragments arranged by size: 1327 168 146 AvrII C'CTAG_G Cuts at: 0 1566 1641 Size: 1566 75 BamHI G'GATC_C Cuts at: 0 1441 1641 Size: 1441 200 BanII G_rGCy'C Cuts at: 0 742 794 999 1641 Size: 742 52 205 642 Fragments arranged by size: 742 642 205 52 BbsI GAAGACnn'nnnn_ Cuts at: 0 629 713 1096 1641 Size: 629 84 383 545 Fragments arranged by size: 629 545 383 84 BciVI GTATCCnnnnn_n' Cuts at: 0 192 1641 Size: 192 1449 BglI GCCn_nnn'nGGC Cuts at: 0 1003 1641 Size: 1003 638 Bme1580I G_kGCm'C Cuts at: 0 197 999 1023 1254 1641 Size: 197 802 24 231 387 Fragments arranged by size: 802 387 231 197 24 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 752 1641 Size: 235 517 889 Fragments arranged by size: 889 517 235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 698 1603 1641 Size: 698 905 38 Fragments arranged by size: 905 698 38 BsaHI Gr'CG_yC Cuts at: 0 1093 1641 Size: 1093 548 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 637 1054 1641 Size: 637 417 587 Fragments arranged by size: 637 587 417 BseYI C'CCAG_C Cuts at: 0 60 488 520 640 1641 Size: 60 428 32 120 1001 Fragments arranged by size: 1001 428 120 60 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 536 1641 Size: 536 1105 BsiHKAI G_wGCw'C Cuts at: 0 197 1641 Size: 197 1444 BsmI GAATG_Cn' Cuts at: 0 618 1457 1641 Size: 618 839 184 Fragments arranged by size: 839 618 184 Bsp1286I G_dGCh'C Cuts at: 0 197 742 794 999 1023 1254 1641 Size: 197 545 52 205 24 231 387 Fragments arranged by size: 545 387 231 205 197 52 24 BspHI T'CATG_A Cuts at: 0 1217 1641 Size: 1217 424 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 275 1641 Size: 275 1366 BsrGI T'GTAC_A Cuts at: 0 140 1641 Size: 140 1501 BstAPI GCAn_nnn'nTGC Cuts at: 0 48 1641 Size: 48 1593 BstYI r'GATC_y Cuts at: 0 503 1441 1641 Size: 503 938 200 Fragments arranged by size: 938 503 200 BtgI C'CryG_G Cuts at: 0 20 980 1641 Size: 20 960 661 Fragments arranged by size: 960 661 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 512 1641 Size: 512 1129 DraIII CAC_nnn'GTG Cuts at: 0 454 1641 Size: 454 1187 EaeI y'GGCC_r Cuts at: 0 668 1641 Size: 668 973 EarI CTCTTCn'nnn_ Cuts at: 0 20 242 626 782 1332 1576 1641 Size: 20 222 384 156 550 244 65 Fragments arranged by size: 550 384 244 222 156 65 20 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 563 752 800 1043 1600 1641 Size: 235 328 189 48 243 557 41 Fragments arranged by size: 557 328 243 235 189 48 41 EcoO109I rG'GnC_Cy Cuts at: 0 995 1082 1641 Size: 995 87 559 Fragments arranged by size: 995 559 87 EcoRV GAT'ATC Cuts at: 0 1293 1641 Size: 1293 348 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 49 593 1641 Size: 49 544 1048 Fragments arranged by size: 1048 544 49 HaeII r_GCGC'y Cuts at: 0 415 1641 Size: 415 1226 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 899 931 1580 1612 1641 Size: 899 32 649 32 29 Fragments arranged by size: 899 649 32 32 29 HincII GTy'rAC Cuts at: 0 599 857 1484 1641 Size: 599 258 627 157 Fragments arranged by size: 627 599 258 157 HindIII A'AGCT_T Cuts at: 0 303 1641 Size: 303 1338 HpaI GTT'AAC Cuts at: 0 599 1484 1641 Size: 599 885 157 Fragments arranged by size: 885 599 157 MscI TGG'CCA Cuts at: 0 670 1641 Size: 670 971 MslI CAynn'nnrTG Cuts at: 0 580 1641 Size: 580 1061 NcoI C'CATG_G Cuts at: 0 20 1641 Size: 20 1621 PflMI CCAn_nnn'nTGG Cuts at: 0 60 1167 1641 Size: 60 1107 474 Fragments arranged by size: 1107 474 60 PfoI T'CCnGG_A Cuts at: 0 560 1641 Size: 560 1081 PpuMI rG'GwC_Cy Cuts at: 0 1082 1641 Size: 1082 559 PspOMI G'GGCC_C Cuts at: 0 995 1641 Size: 995 646 SapI GCTCTTCn'nnn_ Cuts at: 0 782 1641 Size: 782 859 SfcI C'TryA_G Cuts at: 0 162 1641 Size: 162 1479 SmlI C'TyrA_G Cuts at: 0 713 1618 1641 Size: 713 905 23 Fragments arranged by size: 905 713 23 SspI AAT'ATT Cuts at: 0 1329 1641 Size: 1329 312 StuI AGG'CCT Cuts at: 0 107 1641 Size: 107 1534 StyI C'CwwG_G Cuts at: 0 20 734 1566 1641 Size: 20 714 832 75 Fragments arranged by size: 832 714 75 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 223 1641 Size: 223 1418 TatI w'GTAC_w Cuts at: 0 140 1099 1641 Size: 140 959 542 Fragments arranged by size: 959 542 140 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1433 1465 1641 Size: 1433 32 176 Fragments arranged by size: 1433 176 32 Tth111I GACn'n_nGTC Cuts at: 0 535 1641 Size: 535 1106 XbaI T'CTAG_A Cuts at: 0 904 1641 Size: 904 737 XmnI GAAnn'nnTTC Cuts at: 0 576 1641 Size: 576 1065 Enzymes that do cut: AcuI AflIII AleI AlwNI ApaI ApaLI ApoI AvrII BamHI BanII BbsI BciVI BglI Bme1580I BpmI BpuEI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BstAPI BstYI BtgI BtgZI DraIII EaeI EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI MscI MslI NcoI PflMI PfoI PpuMI PspOMI SapI SfcI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AscI AseI AsiSI AvaI BaeI BanI BbeI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XcmI XhoI XmaI ZraI