################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbeI BbvCI BcgI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI DraI Eam1105I EcoNI EcoRI EcoRV FbaI FseI Hin1I HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI PvuI SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### AclI 570 AACGTT AA^CGTT AcsI 885 GAATTT [GA]^AATT[CT] AfaI 369 GTAC GT^AC AlwNI 296 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1442 AGCGCT AGC^GCT ApoI 885 GAATTT [GA]^AATT[CT] Asp700I 298 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 838 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 944 CCCGGG C^[CT]CG[GA]G BalI 777 TGGCCA TGG^CCA BanII 1273 GAGCCC G[GA]GC[CT]^C BbrPI 313 CACGTG CAC^GTG BbsI 1105 GTCTTC GAAGAC(2/6) BciVI 1394 GTATCC GTATCC(6/5) BfaI 911 CTAG C^TAG BglII 1071 AGATCT A^GATCT BpuAI 1105 GTCTTC GAAGAC(2/6) BsgI 1045 GTGCAG GTGCAG(16/14) BsoBI 944 CCCGGG C^[CT]CG[GA]G BspHI 647 TCATGA T^CATGA BsrDI 465 CATTGC GCAATG(2/0) BsrFI 811 ACCGGC [GA]^CCGG[CT] BstAPI 1374 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 789 GGTCACC G^GT[ACGT]ACC BstPI 789 GGTCACC G^GT[ACGT]ACC BstXI 587 CCAGCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 811 ACCGGC [GA]^CCGG[CT] CpoI 1384 CGGACCG CG^G[AT]CCG DraIII 141 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 355 GACAGAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 481 CGGCCG C^GGCCG EarI 1054 CTCTTC CTCTTC(1/4) EclXI 481 CGGCCG C^GGCCG Eco47III 1442 AGCGCT AGC^GCT Eco52I 481 CGGCCG C^GGCCG EcoO65I 789 GGTCACC G^GT[ACGT]ACC EcoT22I 1235 ATGCAT ATGCA^T HaeII 1442 AGCGCT [GA]GCGC^[CT] HgaI 167 GCGTC GACGC(5/10) Ksp632I 1054 CTCTTC CTCTTC(1/4) KspI 669 CCGCGG CCGC^GG MaeI 911 CTAG C^TAG MluNI 777 TGGCCA TGG^CCA MscI 777 TGGCCA TGG^CCA MseI 374 TTAA T^TAA NcoI 695 CCATGG C^CATGG NsiI 1235 ATGCAT ATGCA^T PflFI 838 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 313 CACGTG CAC^GTG PmlI 313 CACGTG CAC^GTG PpuMI 1063 GGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 570 AACGTT AA^CGTT PstI 784 CTGCAG CTGCA^G RcaI 647 TCATGA T^CATGA RsaI 369 GTAC GT^AC RsrII 1384 CGGACCG CG^G[AT]CCG SacII 669 CCGCGG CCGC^GG SapI 1053 GCTCTTC GCTCTTC(1/4) ScaI 368 AGTACT AGT^ACT SfcI 784 CTGCAG C^T[GA][CT]AG SmaI 944 CCCGGG CCC^GGG SmlI 1331 CTCAAG C^T[CT][GA]AG Tru9I 374 TTAA T^TAA Tsp509I 886 AATT ^AATT Tth111I 838 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 944 CCCGGG C^CCGGG XmaI 944 CCCGGG C^CCGGG XmnI 298 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 670 CGCG CG^CG 755 CGCG AocI 735 CCTGAGG CC^T[ACGT]AGG 1266 CCTGAGG AspHI 400 GAGCAC G[AT]GC[AT]^C 706 GAGCAC BglI 73 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 152 GCCTCGGCGGC BsaAI 118 TACGTG [CT]AC^GT[GA] 313 CACGTG BsiEI 481 CGGCCG CG[GA][CT]^CG 1100 CGACCG BsiHKAI 400 GAGCAC G[AT]GC[AT]^C 706 GAGCAC BsmFI 23 GGGAC GGGAC(10/14) 865 GTCCC BstUI 670 CGCG CG^CG 755 CGCG Bsu36I 735 CCTGAGG CC^T[ACGT]AGG 1266 CCTGAGG DraII 149 GGGGCCT [GA]G^G[ACGT]CC[CT] 1063 GGGTCCT Eco81I 735 CCTGAGG CC^T[ACGT]AGG 1266 CCTGAGG EcoO109I 149 GGGGCCT [GA]G^G[ACGT]CC[CT] 1063 GGGTCCT HincII 169 GTCAAC GT[CT]^[GA]AC 394 GTTGAC HindII 169 GTCAAC GT[CT]^[GA]AC 394 GTTGAC MvnI 670 CGCG CG^CG 755 CGCG NspI 1176 GCATGT [GA]CATG^[CT] 1405 ACATGC PleI 940 GACTC GAGTC(4/5) 863 GAGTC StuI 222 AGGCCT AGG^CCT 1449 AGGCCT XcmI 977 CCATTGGTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1016 CCACAGCGATCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AluI AlwI AvaII AviII BanI BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII