(Circular) (Six-base) MAPSORT of: pFN21AE5633.seq Check: 6423 from: 1 to: 6181 pFN21AE5633 6181 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2177 3370 2177 Size: 1193 4988 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3524 1937 Size: 1587 4594 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 2576 4308 4681 1563 Size: 1013 1732 373 3063 Fragments arranged by size: 3063 1732 1013 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 2576 4244 5628 5896 1632 Size: 707 237 1668 1384 268 1917 Fragments arranged by size: 1917 1668 1384 707 268 237 AfeI AGC'GCT Cuts at: 3449 3449 Size: 6181 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 5080 6057 1203 Size: 3877 977 1327 Fragments arranged by size: 3877 1327 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 4910 4910 Size: 6181 AleI CACnn'nnGTG Cuts at: 1166 2963 1166 Size: 1797 4384 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6181 AlwNI CAG_nnn'CTG Cuts at: 2306 3924 5496 2306 Size: 1618 1572 2991 Fragments arranged by size: 2991 1618 1572 ApaLI G'TGCA_C Cuts at: 4238 5394 4238 Size: 1156 5025 ApoI r'AATT_y Cuts at: 1493 2890 3490 3716 3868 5977 1493 Size: 1397 600 226 152 2109 1697 Fragments arranged by size: 2109 1697 1397 600 226 152 AseI AT'TA_AT Cuts at: 160 1032 4735 160 Size: 872 3703 1606 Fragments arranged by size: 3703 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 2949 3506 1870 Size: 73 1006 557 4545 Fragments arranged by size: 4545 1006 557 73 BamHI G'GATC_C Cuts at: 3511 3878 3511 Size: 367 5814 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2165 2513 2729 2834 Size: 325 217 627 357 21 348 216 105 Cuts at: 2834 3502 4957 618 Size: 668 1455 1842 Fragments arranged by size: 1842 1455 668 627 357 348 325 217 216 105 21 BanII G_rGCy'C Cuts at: 1391 3282 3500 1391 Size: 1891 218 4072 Fragments arranged by size: 4072 1891 218 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3103 928 Size: 971 1204 4006 Fragments arranged by size: 4006 1204 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4372 4406 1903 Size: 34 2435 34 3678 Fragments arranged by size: 3678 2435 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4073 5283 3410 Size: 663 1210 4308 Fragments arranged by size: 4308 1210 663 BclI T'GATC_A Cuts at: 1548 1548 Size: 6181 BfrBI ATG'CAT Cuts at: 3242 3242 Size: 6181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2084 2163 4792 Size: 107 122 71 631 605 412 79 2629 Cuts at: 4792 136 Size: 1525 Fragments arranged by size: 2629 1525 631 605 412 122 107 79 71 BglII A'GATC_T Cuts at: 3076 6176 3076 Size: 3100 3081 BlpI GC'TnA_GC Cuts at: 3598 3598 Size: 6181 Bme1580I G_kGCm'C Cuts at: 1790 2755 2837 2970 4242 5398 1790 Size: 965 82 133 1272 1156 2573 Fragments arranged by size: 2573 1272 1156 965 133 82 BmgBI CAC'GTC Cuts at: 1318 2992 1318 Size: 1674 4507 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2231 2344 3241 Size: 416 53 83 399 24 814 113 897 Cuts at: 3241 3912 4870 442 Size: 671 958 1753 Fragments arranged by size: 1753 958 897 814 671 416 399 113 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2308 2499 2627 3143 3236 4841 1239 Size: 1069 191 128 516 93 1605 2579 Fragments arranged by size: 2579 1605 1069 516 191 128 93 Bpu10I CC'TnA_GC Cuts at: 3009 3451 3009 Size: 442 5739 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3321 4306 5171 5469 3321 Size: 985 865 298 4033 Fragments arranged by size: 4033 985 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4844 882 Size: 617 3345 2219 Fragments arranged by size: 3345 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2125 2320 3972 493 Size: 711 921 195 1652 2702 Fragments arranged by size: 2702 1652 921 711 195 BsaBI GATnn'nnATC Cuts at: 6175 6175 Size: 6181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3988 4370 5005 275 Size: 53 83 186 936 2455 382 635 1451 Fragments arranged by size: 2455 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4614 5012 5286 5433 5844 4614 Size: 398 274 147 411 4951 Fragments arranged by size: 4951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5956 Fragments arranged by size: 5956 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3164 3167 3434 1186 Size: 1978 3 267 3933 Fragments arranged by size: 3933 1978 267 3 BseYI C'CCAG_C Cuts at: 2091 2095 2256 2268 2903 3295 5384 5880 2091 Size: 4 161 12 635 392 2089 496 2392 Fragments arranged by size: 2392 2089 635 496 392 161 12 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3071 1163 Size: 573 1335 4273 Fragments arranged by size: 4273 1335 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2489 3108 3631 4392 5420 1540 Size: 275 186 488 619 523 761 1028 2301 Fragments arranged by size: 2301 1028 761 619 523 488 275 186 BsiHKAI G_wGCw'C Cuts at: 2409 2715 4242 4327 5398 2409 Size: 306 1527 85 1071 3192 Fragments arranged by size: 3192 1527 1071 306 85 BsmI GAATG_Cn' Cuts at: 3692 3785 3692 Size: 93 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2409 2715 2755 2837 2970 3282 3500 Size: 399 619 306 40 82 133 312 218 Cuts at: 3500 4242 4327 5398 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 619 399 312 306 218 133 85 82 40 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 2652 4070 5800 2652 Size: 1418 1730 3033 Fragments arranged by size: 3033 1730 1418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2009 2177 2782 3370 3536 844 Size: 1165 168 605 588 166 3489 Fragments arranged by size: 3489 1165 605 588 168 166 BsrBI CCG'CTC Cuts at: 1394 1823 4068 1394 Size: 429 2245 3507 Fragments arranged by size: 3507 2245 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2469 4676 4850 65 Size: 1183 1221 2207 174 1396 Fragments arranged by size: 2207 1396 1221 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2816 4825 5012 1700 Size: 110 1006 2009 187 2869 Fragments arranged by size: 2869 2009 1006 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 4241 5253 4241 Size: 1012 5169 BstAPI GCAn_nnn'nTGC Cuts at: 794 3385 794 Size: 2591 3590 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 2794 1184 Size: 1610 4571 BstXI CCAn_nnnn'nTGG Cuts at: 2599 2599 Size: 6181 BstYI r'GATC_y Cuts at: 1972 2057 2934 3076 3511 3878 4263 4280 5732 Size: 85 877 142 435 367 385 17 1452 Cuts at: 5732 5818 5830 6176 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 877 435 385 367 346 142 86 85 17 12 Bsu36I CC'TnA_GG Cuts at: 2741 3272 2741 Size: 531 5650 BtgI C'CryG_G Cuts at: 513 1065 2004 2112 2674 2700 3169 3393 3465 Size: 552 939 108 562 26 469 224 72 Cuts at: 3465 513 Size: 3229 Fragments arranged by size: 3229 939 562 552 469 224 108 72 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5442 Fragments arranged by size: 5442 683 56 BtsI GCAGTG_nn' Cuts at: 3165 3697 4491 4511 3165 Size: 532 794 20 4835 Fragments arranged by size: 4835 794 532 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DraIII CAC_nnn'GTG Cuts at: 2151 2151 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2366 5188 809 Size: 507 1050 2822 1802 Fragments arranged by size: 2822 1802 1050 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2486 2782 Size: 54 1425 50 275 18 279 377 296 Cuts at: 2782 3033 3628 4517 6146 8 Size: 251 595 889 1629 43 Fragments arranged by size: 1629 1425 889 595 377 296 279 275 251 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2486 3628 1537 Size: 275 674 1142 4090 Fragments arranged by size: 4090 1142 674 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3065 4111 1353 Size: 1712 1046 3423 Fragments arranged by size: 3423 1712 1046 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4768 5142 5288 387 Size: 1064 138 3179 374 146 1280 Fragments arranged by size: 3179 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2308 2339 2499 2576 2627 3143 3236 Size: 393 676 31 160 77 51 516 93 Cuts at: 3236 4244 4841 5628 5896 1239 Size: 1008 597 787 268 1524 Fragments arranged by size: 1524 1008 787 676 597 516 393 268 160 93 77 51 31 EcoO109I rG'GnC_Cy Cuts at: 2155 3069 2155 Size: 914 5267 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 FspI TGC'GCA Cuts at: 2660 3378 3437 4687 2660 Size: 718 59 1250 4154 Fragments arranged by size: 4154 1250 718 59 HaeII r_GCGC'y Cuts at: 1678 3451 5328 1678 Size: 1773 1877 2531 Fragments arranged by size: 2531 1877 1773 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2217 2249 4822 4854 4896 Size: 32 135 32 645 32 2573 32 42 Cuts at: 4896 4928 1373 Size: 32 2626 Fragments arranged by size: 2626 2573 645 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2176 2401 3525 3771 669 Size: 988 281 238 225 1124 246 3079 Fragments arranged by size: 3079 1124 988 281 246 238 225 HindIII A'AGCT_T Cuts at: 748 748 Size: 6181 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5295 5479 1305 Size: 3990 184 2007 Fragments arranged by size: 3990 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2784 10 Size: 54 1425 343 952 3407 Fragments arranged by size: 3407 1425 952 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2651 2654 2963 3247 4139 4498 4657 Size: 648 1485 3 309 284 892 359 159 Cuts at: 4657 518 Size: 2042 Fragments arranged by size: 2042 1485 892 648 359 309 284 159 3 MspA1I CmG'CkG Cuts at: 1606 2026 2095 2676 2788 3055 3289 3597 4274 Size: 420 69 581 112 267 234 308 677 Cuts at: 4274 5422 5667 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 581 420 308 267 245 234 112 69 NcoI C'CATG_G Cuts at: 513 1065 2004 2700 513 Size: 552 939 696 3994 Fragments arranged by size: 3994 939 696 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NsiI A_TGCA'T Cuts at: 3244 3244 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 3185 3414 3539 5084 1132 Size: 97 1956 229 125 1545 2229 Fragments arranged by size: 2229 1956 1545 229 125 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6181 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6181 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PmlI CAC'GTG Cuts at: 2320 2320 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4250 4282 5763 5795 4250 Size: 32 1481 32 4636 Fragments arranged by size: 4636 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3069 3069 Size: 6181 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3207 3751 3207 Size: 544 5637 PstI C_TGCA'G Cuts at: 830 2793 3533 830 Size: 1963 740 3478 Fragments arranged by size: 3478 1963 740 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2095 2788 3289 2095 Size: 693 501 4987 Fragments arranged by size: 4987 693 501 RsrII CG'GwC_CG Cuts at: 3390 3390 Size: 6181 SacII CC_GC'GG Cuts at: 2677 2677 Size: 6181 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SapI GCTCTTCn'nnn_ Cuts at: 3065 3065 Size: 6181 SbfI CC_TGCA'GG Cuts at: 3533 3533 Size: 6181 ScaI AGT'ACT Cuts at: 2375 4429 6141 2375 Size: 2054 1712 2415 Fragments arranged by size: 2415 2054 1712 SfcI C'TryA_G Cuts at: 826 1045 2789 3529 4664 5345 5536 826 Size: 219 1744 740 1135 681 191 1471 Fragments arranged by size: 1744 1471 1135 740 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 2951 3508 1872 Size: 1079 557 4545 Fragments arranged by size: 4545 1079 557 SmlI C'TyrA_G Cuts at: 820 1017 1943 3336 4285 5186 5448 820 Size: 197 926 1393 949 901 262 1553 Fragments arranged by size: 1553 1393 949 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 3539 1132 Size: 2407 3774 SspI AAT'ATT Cuts at: 5 52 4105 5 Size: 47 4053 2081 Fragments arranged by size: 4053 2081 47 StuI AGG'CCT Cuts at: 1517 2229 3456 1517 Size: 712 1227 4242 Fragments arranged by size: 4242 1227 712 StyI C'CwwG_G Cuts at: 513 1065 2004 2079 2140 2517 2700 3310 3620 Size: 552 939 75 61 377 183 610 310 Cuts at: 3620 513 Size: 3074 Fragments arranged by size: 3074 939 610 552 377 310 183 75 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3034 4208 4225 4378 4563 5008 325 Size: 1255 1454 1174 17 153 185 445 1498 Fragments arranged by size: 1498 1454 1255 1174 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2373 4427 6139 Size: 274 80 33 51 1443 396 2054 1712 Cuts at: 6139 96 Size: 138 Fragments arranged by size: 2054 1712 1443 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2091 2123 2309 2341 3346 3378 6141 6173 2091 Size: 32 186 32 1005 32 2763 32 2099 Fragments arranged by size: 2763 2099 1005 186 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2846 1316 Size: 1530 4651 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2989 3028 2989 Size: 39 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6181 XmaI C'CCGG_G Cuts at: 1870 2949 3506 1870 Size: 1079 557 4545 Fragments arranged by size: 4545 1079 557 XmnI GAAnn'nnTTC Cuts at: 2307 4310 2307 Size: 2003 4178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI SacI SanDI SexAI SfiI SfoI SrfI SwaI