(Linear) (Six-base) MAPSORT of: KIEE5633.seq Check: 2578 from: 1 to: 1476 KIEE5633.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 172 1365 1476 Size: 172 1193 111 Fragments arranged by size: 1193 172 111 AclI AA'CG_TT Cuts at: 0 571 1476 Size: 571 905 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 571 1476 Size: 334 237 905 Fragments arranged by size: 905 334 237 AfeI AGC'GCT Cuts at: 0 1444 1476 Size: 1444 32 AleI CACnn'nnGTG Cuts at: 0 958 1476 Size: 958 518 AlwNI CAG_nnn'CTG Cuts at: 0 301 1476 Size: 301 1175 ApoI r'AATT_y Cuts at: 0 885 1476 Size: 885 591 AvaI C'yCGr_G Cuts at: 0 944 1476 Size: 944 532 BanI G'GyrC_C Cuts at: 0 139 160 508 724 829 1476 Size: 139 21 348 216 105 647 Fragments arranged by size: 647 348 216 139 105 21 BanII G_rGCy'C Cuts at: 0 1277 1476 Size: 1277 199 BbsI GAAGACnn'nnnn_ Cuts at: 0 1098 1476 Size: 1098 378 BciVI GTATCCnnnnn_n' Cuts at: 0 1405 1476 Size: 1405 71 BfrBI ATG'CAT Cuts at: 0 1237 1476 Size: 1237 239 BglI GCCn_nnn'nGGC Cuts at: 0 79 158 1476 Size: 79 79 1318 Fragments arranged by size: 1318 79 79 BglII A'GATC_T Cuts at: 0 1071 1476 Size: 1071 405 Bme1580I G_kGCm'C Cuts at: 0 750 832 965 1476 Size: 750 82 133 511 Fragments arranged by size: 750 511 133 82 BmgBI CAC'GTC Cuts at: 0 987 1476 Size: 987 489 BmrI ACTGGGnnnn_n' Cuts at: 0 226 339 1236 1476 Size: 226 113 897 240 Fragments arranged by size: 897 240 226 113 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 303 494 622 1138 1231 1476 Size: 303 191 128 516 93 245 Fragments arranged by size: 516 303 245 191 128 93 Bpu10I CC'TnA_GC Cuts at: 0 1004 1446 1476 Size: 1004 442 30 Fragments arranged by size: 1004 442 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1316 1476 Size: 1316 160 BsaAI yAC'GTr Cuts at: 0 120 315 1476 Size: 120 195 1161 Fragments arranged by size: 1161 195 120 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1159 1162 1429 1476 Size: 1159 3 267 47 Fragments arranged by size: 1159 267 47 3 BseYI C'CCAG_C Cuts at: 0 86 90 251 263 898 1290 1476 Size: 86 4 161 12 635 392 186 Fragments arranged by size: 635 392 186 161 86 12 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1066 1476 Size: 1066 410 BsiEI CG_ry'CG Cuts at: 0 484 1103 1476 Size: 484 619 373 Fragments arranged by size: 619 484 373 BsiHKAI G_wGCw'C Cuts at: 0 404 710 1476 Size: 404 306 766 Fragments arranged by size: 766 404 306 Bsp1286I G_dGCh'C Cuts at: 0 404 710 750 832 965 1277 1476 Size: 404 306 40 82 133 312 199 Fragments arranged by size: 404 312 306 199 133 82 40 BspHI T'CATG_A Cuts at: 0 647 1476 Size: 647 829 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 4 172 777 1365 1476 Size: 4 168 605 588 111 Fragments arranged by size: 605 588 168 111 4 BsrDI GCAATG_nn' Cuts at: 0 464 1476 Size: 464 1012 BsrFI r'CCGG_y Cuts at: 0 811 1476 Size: 811 665 BstAPI GCAn_nnn'nTGC Cuts at: 0 1380 1476 Size: 1380 96 BstEII G'GTnAC_C Cuts at: 0 789 1476 Size: 789 687 BstXI CCAn_nnnn'nTGG Cuts at: 0 594 1476 Size: 594 882 BstYI r'GATC_y Cuts at: 0 52 929 1071 1476 Size: 52 877 142 405 Fragments arranged by size: 877 405 142 52 Bsu36I CC'TnA_GG Cuts at: 0 736 1267 1476 Size: 736 531 209 Fragments arranged by size: 736 531 209 BtgI C'CryG_G Cuts at: 0 107 669 695 1164 1388 1460 1476 Size: 107 562 26 469 224 72 16 Fragments arranged by size: 562 469 224 107 72 26 16 BtsI GCAGTG_nn' Cuts at: 0 1160 1476 Size: 1160 316 DraIII CAC_nnn'GTG Cuts at: 0 146 1476 Size: 146 1330 DrdI GACnn_nn'nnGTC Cuts at: 0 361 1476 Size: 361 1115 EaeI y'GGCC_r Cuts at: 0 104 481 777 1028 1476 Size: 104 377 296 251 448 Fragments arranged by size: 448 377 296 251 104 EagI C'GGCC_G Cuts at: 0 481 1476 Size: 481 995 EarI CTCTTCn'nnn_ Cuts at: 0 1060 1476 Size: 1060 416 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 303 334 494 571 622 1138 1231 1476 Size: 303 31 160 77 51 516 93 245 Fragments arranged by size: 516 303 245 160 93 77 51 31 EcoO109I rG'GnC_Cy Cuts at: 0 150 1064 1476 Size: 150 914 412 Fragments arranged by size: 914 412 150 FspI TGC'GCA Cuts at: 0 655 1373 1432 1476 Size: 655 718 59 44 Fragments arranged by size: 718 655 59 44 HaeII r_GCGC'y Cuts at: 0 1446 1476 Size: 1446 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 212 244 1476 Size: 212 32 1232 Fragments arranged by size: 1232 212 32 HincII GTy'rAC Cuts at: 0 171 396 1476 Size: 171 225 1080 Fragments arranged by size: 1080 225 171 MscI TGG'CCA Cuts at: 0 779 1476 Size: 779 697 MslI CAynn'nnrTG Cuts at: 0 646 649 958 1242 1476 Size: 646 3 309 284 234 Fragments arranged by size: 646 309 284 234 3 MspA1I CmG'CkG Cuts at: 0 21 90 671 783 1050 1284 1476 Size: 21 69 581 112 267 234 192 Fragments arranged by size: 581 267 234 192 112 69 21 NcoI C'CATG_G Cuts at: 0 695 1476 Size: 695 781 NsiI A_TGCA'T Cuts at: 0 1239 1476 Size: 1239 237 NspI r_CATG'y Cuts at: 0 1180 1409 1476 Size: 1180 229 67 Fragments arranged by size: 1180 229 67 PmlI CAC'GTG Cuts at: 0 315 1476 Size: 315 1161 PpuMI rG'GwC_Cy Cuts at: 0 1064 1476 Size: 1064 412 PsiI TTA'TAA Cuts at: 0 1202 1476 Size: 1202 274 PstI C_TGCA'G Cuts at: 0 788 1476 Size: 788 688 PvuII CAG'CTG Cuts at: 0 90 783 1284 1476 Size: 90 693 501 192 Fragments arranged by size: 693 501 192 90 RsrII CG'GwC_CG Cuts at: 0 1385 1476 Size: 1385 91 SacII CC_GC'GG Cuts at: 0 672 1476 Size: 672 804 SapI GCTCTTCn'nnn_ Cuts at: 0 1060 1476 Size: 1060 416 ScaI AGT'ACT Cuts at: 0 370 1476 Size: 370 1106 SfcI C'TryA_G Cuts at: 0 784 1476 Size: 784 692 SmaI CCC'GGG Cuts at: 0 946 1476 Size: 946 530 SmlI C'TyrA_G Cuts at: 0 1331 1476 Size: 1331 145 StuI AGG'CCT Cuts at: 0 224 1451 1476 Size: 224 1227 25 Fragments arranged by size: 1227 224 25 StyI C'CwwG_G Cuts at: 0 74 135 512 695 1305 1476 Size: 74 61 377 183 610 171 Fragments arranged by size: 610 377 183 171 74 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1029 1476 Size: 1029 447 TatI w'GTAC_w Cuts at: 0 368 1476 Size: 368 1108 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 86 118 304 336 1341 1373 1476 Size: 86 32 186 32 1005 32 103 Fragments arranged by size: 1005 186 103 86 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 841 1476 Size: 841 635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 984 1023 1476 Size: 984 39 453 Fragments arranged by size: 984 453 39 XmaI C'CCGG_G Cuts at: 0 944 1476 Size: 944 532 XmnI GAAnn'nnTTC Cuts at: 0 302 1476 Size: 302 1174 Enzymes that do cut: AarI AclI AcuI AfeI AleI AlwNI ApoI AvaI BanI BanII BbsI BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI Eco57MI EcoO109I FspI HaeII Hin4I HincII MscI MslI MspA1I NcoI NsiI NspI PmlI PpuMI PsiI PstI PvuII RsrII SacII SapI ScaI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BlpI BmtI BplI BsaI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI SacI SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI