################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII AccI AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BbrPI BcgI BciVI BclI BfrI BlnI BsaAI BsaBI BsaI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI Tth111I TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 302 GGTACC G^GTACC AccIII 212 TCCGGA T^CCGGA AcyI 219 GGCGCC G[GA]^CG[CT]C AflIII 1393 ACATGT A^C[GA][CT]GT Asp718I 302 GGTACC G^GTACC AvaI 237 CCCGGG C^[CT]CG[GA]G BalI 128 TGGCCA TGG^CCA BamHI 1343 GGATCC G^GATCC BbeI 219 GGCGCC GGCGC^C BbvCI 489 GCTGAGG CCTCAGC(-5/-2) BfaI 1015 CTAG C^TAG BglI 1164 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 231 GCTGAGC GC^T[ACGT]AGC Bpu1102I 231 GCTGAGC GC^T[ACGT]AGC BsaHI 219 GGCGCC G[GA]^CG[CT]C BsaWI 212 TCCGGA [AT]^CCGG[AT] BseAI 212 TCCGGA T^CCGGA BseRI 595 GAGGAG GAGGAG(10/8) BsoBI 237 CCCGGG C^[CT]CG[GA]G BspEI 212 TCCGGA T^CCGGA BspLU11I 1393 ACATGT A^CATGT BspMI 602 ACCTGC ACCTGC(4/8) BssSI 355 CACGAG CACGAG(-5/-1) BstXI 393 CCAAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 231 GCTGAGC GC^T[ACGT]AGC CfoI 220 GCGC GCG^C DrdI 1597 GACAGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 1001 CCAAGG C^C[AT][AT]GG HaeII 219 GGCGCC [GA]GCGC^[CT] HgaI 178 GCGTC GACGC(5/10) HhaI 220 GCGC GCG^C Hin1I 219 GGCGCC G[GA]^CG[CT]C HinP1I 220 GCGC G^CGC HincII 982 GTCAAC GT[CT]^[GA]AC HindII 982 GTCAAC GT[CT]^[GA]AC KasI 219 GGCGCC G^GCGCC KpnI 302 GGTACC GGTAC^C MaeI 1015 CTAG C^TAG MaeII 317 ACGT A^CGT MluNI 128 TGGCCA TGG^CCA MroI 212 TCCGGA T^CCGGA MscI 128 TGGCCA TGG^CCA MseI 445 TTAA T^TAA MspA1I 163 CAGCTG C[AC]G^C[GT]G NarI 219 GGCGCC GG^CGCC PflMI 790 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 163 CAGCTG CAG^CTG SacI 1081 GAGCTC GAGCT^C ScaI 1559 AGTACT AGT^ACT SfoI 219 GGCGCC GGC^GCC SmaI 237 CCCGGG CCC^GGG SmlI 1255 CTCAAG C^T[CT][GA]AG SspI 1235 AATATT AAT^ATT StuI 672 AGGCCT AGG^CCT StyI 1001 CCAAGG C^C[AT][AT]GG Tru9I 445 TTAA T^TAA Van91I 790 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 237 CCCGGG C^CCGGG XmaI 237 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 81 CGCG CG^CG 363 CGCG AcsI 1114 AAATTT [GA]^AATT[CT] 1291 AAATTT AocI 31 CCTCAGG CC^T[ACGT]AGG 481 CCTGAGG ApaI 841 GGGCCC GGGCC^C 1171 GGGCCC ApoI 1114 AAATTT [GA]^AATT[CT] 1291 AAATTT AspHI 640 GAGCAC G[AT]GC[AT]^C 1081 GAGCTC BglII 381 AGATCT A^GATCT 911 AGATCT BsiHKAI 640 GAGCAC G[AT]GC[AT]^C 1081 GAGCTC BspHI 1362 TCATGA T^CATGA 1701 TCATGA BsrFI 1334 ACCGGC [GA]^CCGG[CT] 1373 ACCGGC BstAPI 175 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1579 GCATCCCATGC BstUI 81 CGCG CG^CG 363 CGCG Bsu36I 31 CCTCAGG CC^T[ACGT]AGG 481 CCTGAGG Cfr10I 1334 ACCGGC [GA]^CCGG[CT] 1373 ACCGGC DsaI 134 CCGTGG C^C[GA][CT]GG 298 CCGTGG EarI 710 CTCTTC CTCTTC(1/4) 1180 GAAGAG Eco81I 31 CCTCAGG CC^T[ACGT]AGG 481 CCTGAGG HphI 781 GGTGA GGTGA(8/7) 517 TCACC Ksp632I 710 CTCTTC CTCTTC(1/4) 1180 GAAGAG MvnI 81 CGCG CG^CG 363 CGCG NspI 1393 ACATGT [GA]CATG^[CT] 1436 ACATGC PpuMI 493 AGGACCT [GA]G^G[AT]CC[CT] 599 AGGACCT PstI 807 CTGCAG CTGCA^G 1187 CTGCAG RcaI 1362 TCATGA T^CATGA 1701 TCATGA TfiI 706 GATTC G^A[AT]TC 1065 GATTC Tsp45I 625 GTCAC ^GT[GC]AC 1144 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI Asp700I AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp509I TspRI XcmI XhoII XmnI