(Circular) (Six-base) MAPSORT of: pFN21AB7877.seq Check: 5555 from: 1 to: 6418 pFN21AB7877 6418 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4228 278 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3761 1937 Size: 1824 4594 Acc65I G'GTAC_C Cuts at: 2307 3739 2307 Size: 1432 4986 AclI AA'CG_TT Cuts at: 1563 4545 4918 1563 Size: 2982 373 3063 Fragments arranged by size: 3063 2982 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3128 3204 4481 5865 6133 1632 Size: 1496 76 1277 1384 268 1917 Fragments arranged by size: 1917 1496 1384 1277 268 76 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6221 AflIII A'CryG_T Cuts at: 1203 3398 5317 6294 1203 Size: 2195 1919 977 1327 Fragments arranged by size: 2195 1919 1327 977 AgeI A'CCGG_T Cuts at: 5249 5249 Size: 6418 AhdI GACnn_n'nnGTC Cuts at: 5147 5147 Size: 6418 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6418 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6418 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2963 2995 2963 Size: 32 6386 AlwNI CAG_nnn'CTG Cuts at: 2080 2186 3654 4161 5733 2080 Size: 106 1468 507 1572 2765 Fragments arranged by size: 2765 1572 1468 507 106 ApaI G_GGCC'C Cuts at: 2850 3180 2850 Size: 330 6088 ApaLI G'TGCA_C Cuts at: 4475 5631 4475 Size: 1156 5262 ApoI r'AATT_y Cuts at: 1493 3119 3296 3727 3953 4105 6214 1493 Size: 1626 177 431 226 152 2109 1697 Fragments arranged by size: 2109 1697 1626 431 226 177 152 AseI AT'TA_AT Cuts at: 160 1032 4972 160 Size: 872 3940 1606 Fragments arranged by size: 3940 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 AvaI C'yCGr_G Cuts at: 1870 1943 2242 3743 1870 Size: 73 299 1501 4545 Fragments arranged by size: 4545 1501 299 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2291 2324 2291 Size: 33 6385 BamHI G'GATC_C Cuts at: 3348 3748 4115 3348 Size: 400 367 5651 Fragments arranged by size: 5651 400 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2224 2307 2372 3739 5194 Size: 325 217 627 437 83 65 1367 1455 Cuts at: 5194 618 Size: 1842 Fragments arranged by size: 1842 1455 1367 627 437 325 217 83 65 BanII G_rGCy'C Cuts at: 1391 2243 2850 3090 3180 3737 1391 Size: 852 607 240 90 557 4072 Fragments arranged by size: 4072 852 607 557 240 90 BbeI G_GCGC'C Cuts at: 2228 2228 Size: 6418 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2154 2742 2891 3414 3717 928 Size: 971 255 588 149 523 303 3629 Fragments arranged by size: 3629 971 588 523 303 255 149 BbvCI CC'TCA_GC Cuts at: 2495 2495 Size: 6418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4609 4643 1903 Size: 34 2672 34 3678 Fragments arranged by size: 3678 2672 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4310 5520 4310 Size: 1210 5208 BclI T'GATC_A Cuts at: 1548 1548 Size: 6418 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3175 5029 136 Size: 107 122 71 631 605 1503 1854 1525 Fragments arranged by size: 1854 1525 1503 631 605 122 107 71 BglII A'GATC_T Cuts at: 2386 2916 6413 2386 Size: 530 3497 2391 Fragments arranged by size: 3497 2391 530 BlpI GC'TnA_GC Cuts at: 2237 3835 2237 Size: 1598 4820 Bme1580I G_kGCm'C Cuts at: 1790 2850 2998 3180 4479 5635 1790 Size: 1060 148 182 1299 1156 2573 Fragments arranged by size: 2573 1299 1156 1060 182 148 BmgBI CAC'GTC Cuts at: 1318 2323 1318 Size: 1005 5413 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2500 4149 5107 Size: 416 53 83 399 24 1083 1649 958 Cuts at: 5107 442 Size: 1753 Fragments arranged by size: 1753 1649 1083 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6418 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2283 2283 Size: 6418 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2663 3477 3490 5078 1239 Size: 953 471 814 13 1588 2579 Fragments arranged by size: 2579 1588 953 814 471 13 Bpu10I CC'TnA_GC Cuts at: 2348 2495 2348 Size: 147 6271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3245 4543 5408 5706 3245 Size: 1298 865 298 3957 Fragments arranged by size: 3957 1298 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5081 882 Size: 617 3582 2219 Fragments arranged by size: 3582 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4209 493 Size: 711 3005 2702 Fragments arranged by size: 3005 2702 711 BsaBI GATnn'nnATC Cuts at: 6412 6412 Size: 6418 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2225 4225 4607 5242 Size: 53 83 186 936 692 2000 382 635 Cuts at: 5242 275 Size: 1451 Fragments arranged by size: 2000 1451 936 692 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2217 4851 5249 5523 5670 6081 2217 Size: 2634 398 274 147 411 2554 Fragments arranged by size: 2634 2554 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6193 Fragments arranged by size: 6193 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2615 1186 Size: 1429 4989 BseYI C'CCAG_C Cuts at: 2525 2636 2650 5621 6117 2525 Size: 111 14 2971 496 2826 Fragments arranged by size: 2971 2826 496 111 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2594 2645 3050 1163 Size: 573 858 51 405 4531 Fragments arranged by size: 4531 858 573 405 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3868 4629 5657 1540 Size: 275 186 1867 761 1028 2301 Fragments arranged by size: 2301 1867 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2649 3090 4479 4564 5635 2649 Size: 441 1389 85 1071 3432 Fragments arranged by size: 3432 1389 1071 441 85 BsmI GAATG_Cn' Cuts at: 3929 4022 3929 Size: 93 6325 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2243 2649 2850 2998 3090 3180 3737 Size: 399 453 406 201 148 92 90 557 Cuts at: 3737 4479 4564 5635 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 557 453 406 399 201 148 92 90 85 BspEI T'CCGG_A Cuts at: 2217 6081 2217 Size: 3864 2554 BspHI T'CATG_A Cuts at: 3367 3706 4307 6037 3367 Size: 339 601 1730 3748 Fragments arranged by size: 3748 1730 601 339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2616 3773 844 Size: 1772 1157 3489 Fragments arranged by size: 3489 1772 1157 BsrBI CCG'CTC Cuts at: 1394 1823 4305 1394 Size: 429 2482 3507 Fragments arranged by size: 3507 2482 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4913 5087 65 Size: 1183 3665 174 1396 Fragments arranged by size: 3665 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3339 3378 5062 5249 1700 Size: 110 1529 39 1684 187 2869 Fragments arranged by size: 2869 1684 1529 187 110 39 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6418 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6418 BssSI C'ACGA_G Cuts at: 2360 4478 5490 2360 Size: 2118 1012 3288 Fragments arranged by size: 3288 2118 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2186 3590 794 Size: 1392 1404 3622 Fragments arranged by size: 3622 1404 1392 BstBI TT'CG_AA Cuts at: 5222 5222 Size: 6418 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6418 BstXI CCAn_nnnn'nTGG Cuts at: 2405 2405 Size: 6418 BstYI r'GATC_y Cuts at: 1972 2386 2705 2916 3348 3748 4115 4500 4517 Size: 414 319 211 432 400 367 385 17 Cuts at: 4517 5969 6055 6067 6413 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 432 414 400 385 367 346 319 211 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2037 2487 2037 Size: 450 5968 BtgI C'CryG_G Cuts at: 513 1065 2004 2139 2303 513 Size: 552 939 135 164 4628 Fragments arranged by size: 4628 939 552 164 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5679 Fragments arranged by size: 5679 683 56 BtsI GCAGTG_nn' Cuts at: 2386 3038 3934 4728 4748 2386 Size: 652 896 794 20 4056 Fragments arranged by size: 4056 896 794 652 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6418 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2404 2439 3550 3585 529 Size: 35 1840 35 1111 35 3362 Fragments arranged by size: 3362 1840 1111 35 35 35 DraI TTT'AAA Cuts at: 3722 3722 Size: 6418 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3608 5425 809 Size: 507 2292 1817 1802 Fragments arranged by size: 2292 1817 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2018 2082 2133 Size: 54 1425 50 275 18 188 64 51 Cuts at: 2133 3865 4754 6383 8 Size: 1732 889 1629 43 Fragments arranged by size: 1732 1629 1425 889 275 188 64 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3865 1537 Size: 275 2053 4090 Fragments arranged by size: 4090 2053 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2721 3180 4348 1353 Size: 1368 459 1168 3423 Fragments arranged by size: 3423 1368 1168 459 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5005 5379 5525 387 Size: 1064 138 3416 374 146 1280 Fragments arranged by size: 3416 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3088 3088 Size: 6418 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2663 3128 3204 3477 3490 4481 Size: 393 560 471 465 76 273 13 991 Cuts at: 4481 5078 5865 6133 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 991 787 597 560 471 465 393 273 268 76 13 EcoO109I rG'GnC_Cy Cuts at: 2499 2605 2847 3177 2499 Size: 106 242 330 5740 Fragments arranged by size: 5740 330 242 106 EcoRI G'AATT_C Cuts at: 3727 4105 3727 Size: 378 6040 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2759 3107 2759 Size: 348 6070 FspI TGC'GCA Cuts at: 4924 4924 Size: 6418 HaeII r_GCGC'y Cuts at: 1678 2228 5565 1678 Size: 550 3337 2531 Fragments arranged by size: 3337 2531 550 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2212 2230 2244 2262 2723 Size: 32 135 32 640 18 14 18 461 Cuts at: 2723 2755 3626 3658 5059 5091 5133 5165 1373 Size: 32 871 32 1401 32 42 32 2626 Fragments arranged by size: 2626 1401 871 640 461 135 42 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 2989 3762 4008 669 Size: 988 281 1051 773 246 3079 Fragments arranged by size: 3079 1051 988 773 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6418 HpaI GTT'AAC Cuts at: 4008 4008 Size: 6418 KasI G'GCGC_C Cuts at: 2224 2224 Size: 6418 KpnI G_GTAC'C Cuts at: 2311 3743 2311 Size: 1432 4986 MfeI C'AATT_G Cuts at: 4017 4017 Size: 6418 MluI A'CGCG_T Cuts at: 6294 6294 Size: 6418 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2179 2583 5532 5716 1305 Size: 874 404 2949 184 2007 Fragments arranged by size: 2949 2007 874 404 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 10 Size: 54 1425 343 303 4293 Fragments arranged by size: 4293 1425 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2802 3366 3472 4376 4735 4894 518 Size: 648 1636 564 106 904 359 159 2042 Fragments arranged by size: 2042 1636 904 648 564 359 159 106 MspA1I CmG'CkG Cuts at: 1606 2170 3834 4511 5659 5904 1606 Size: 564 1664 677 1148 245 2120 Fragments arranged by size: 2120 1664 1148 677 564 245 NarI GG'CG_CC Cuts at: 2225 2225 Size: 6418 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4927 Fragments arranged by size: 4927 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6418 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6418 NotI GC'GGCC_GC Cuts at: 3865 3865 Size: 6418 NspI r_CATG'y Cuts at: 1132 1229 3402 3445 3776 5321 1132 Size: 97 2173 43 331 1545 2229 Fragments arranged by size: 2229 2173 1545 331 97 43 PasI CC'CwG_GG Cuts at: 1348 2328 1348 Size: 980 5438 PciI A'CATG_T Cuts at: 3398 5317 3398 Size: 1919 4499 PflMI CCAn_nnn'nTGG Cuts at: 1108 2801 1108 Size: 1693 4725 PmeI GTTT'AAAC Cuts at: 3722 3722 Size: 6418 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4487 4519 6000 6032 4487 Size: 32 1481 32 4873 Fragments arranged by size: 4873 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2499 2605 2499 Size: 106 6312 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6418 PsiI TTA'TAA Cuts at: 3988 3988 Size: 6418 PspOMI G'GGCC_C Cuts at: 2846 3176 2846 Size: 330 6088 PstI C_TGCA'G Cuts at: 830 2816 3196 3770 830 Size: 1986 380 574 3478 Fragments arranged by size: 3478 1986 574 380 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6418 PvuII CAG'CTG Cuts at: 2170 2170 Size: 6418 SacI G_AGCT'C Cuts at: 3090 3090 Size: 6418 SalI G'TCGA_C Cuts at: 1936 3760 1936 Size: 1824 4594 SbfI CC_TGCA'GG Cuts at: 3770 3770 Size: 6418 ScaI AGT'ACT Cuts at: 3566 4666 6378 3566 Size: 1100 1712 3606 Fragments arranged by size: 3606 1712 1100 SfcI C'TryA_G Cuts at: 826 1045 2119 2255 2812 3192 3202 3766 4901 Size: 219 1074 136 557 380 10 564 1135 Cuts at: 4901 5582 5773 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1074 681 564 557 380 219 191 136 10 SfoI GGC'GCC Cuts at: 2226 2226 Size: 6418 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6418 SmaI CCC'GGG Cuts at: 1872 2244 3745 1872 Size: 372 1501 4545 Fragments arranged by size: 4545 1501 372 SmlI C'TyrA_G Cuts at: 820 1017 1943 3260 4522 5423 5685 820 Size: 197 926 1317 1262 901 262 1553 Fragments arranged by size: 1553 1317 1262 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4209 493 Size: 3716 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6418 SphI G_CATG'C Cuts at: 1132 3776 1132 Size: 2644 3774 SspI AAT'ATT Cuts at: 5 52 3242 4342 5 Size: 47 3190 1100 2081 Fragments arranged by size: 3190 2081 1100 47 StuI AGG'CCT Cuts at: 1517 2679 1517 Size: 1162 5256 StyI C'CwwG_G Cuts at: 513 1065 2004 3006 3857 513 Size: 552 939 1002 851 3074 Fragments arranged by size: 3074 1002 939 851 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2539 2664 4445 4462 4615 4800 5245 Size: 1255 959 125 1781 17 153 185 445 Cuts at: 5245 325 Size: 1498 Fragments arranged by size: 1781 1498 1255 959 445 185 153 125 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3264 3485 3564 Size: 274 80 33 51 1443 1287 221 79 Cuts at: 3564 4664 6376 96 Size: 1100 1712 138 Fragments arranged by size: 1712 1443 1287 1100 274 221 138 80 79 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2033 2065 2437 2469 6378 6410 2033 Size: 32 372 32 3909 32 2041 Fragments arranged by size: 3909 2041 372 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6418 XbaI T'CTAG_A Cuts at: 3754 3754 Size: 6418 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2402 2405 2948 2402 Size: 3 543 5872 Fragments arranged by size: 5872 543 3 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6418 XmaI C'CCGG_G Cuts at: 1870 2242 3743 1870 Size: 372 1501 4545 Fragments arranged by size: 4545 1501 372 XmnI GAAnn'nnTTC Cuts at: 3410 3551 3665 4547 3410 Size: 141 114 882 5281 Fragments arranged by size: 5281 882 141 114 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4226 276 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI