Restriction map for
pFN21AE3815
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AlwNI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbsI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BpuAI BsaI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BsrI Bst1107I BstAPI BstBI
BstXI BstZ17I CelII ClaI DraI
DraIII DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MfeI MluI MseI MunI NcoI
NdeI NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI RcaI SalI SapI
SbfI ScaI SexAI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 160 AAATTC [GA]^AATT[CT]
AfaI 309 GTAC GT^AC
AocI 943 CCTCAGG CC^T[ACGT]AGG
ApoI 160 AAATTC [GA]^AATT[CT]
BalI 563 TGGCCA TGG^CCA
BamHI 490 GGATCC G^GATCC
BbrPI 763 CACGTG CAC^GTG
BfaI 1354 CTAG C^TAG
BglII 599 AGATCT A^GATCT
BsaAI 763 CACGTG [CT]AC^GT[GA]
BsaBI 594 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 166 CGGCCG CG[GA][CT]^CG
BsmI 353 GCATTC GAATGC(1/-1)
BssSI 708 CTCGTG CACGAG(-5/-1)
BstEII 694 GGTCACC G^GT[ACGT]ACC
BstPI 694 GGTCACC G^GT[ACGT]ACC
Bsu36I 943 CCTCAGG CC^T[ACGT]AGG
CpoI 475 CGGACCG CG^G[AT]CCG
EagI 166 CGGCCG C^GGCCG
EarI 1390 GAAGAG CTCTTC(1/4)
EclXI 166 CGGCCG C^GGCCG
Eco52I 166 CGGCCG C^GGCCG
Eco81I 943 CCTCAGG CC^T[ACGT]AGG
EcoO65I 694 GGTCACC G^GT[ACGT]ACC
EcoT14I 1068 CCAAGG C^C[AT][AT]GG
Ksp632I 1390 GAAGAG CTCTTC(1/4)
KspI 218 CCGCGG CCGC^GG
MaeI 1354 CTAG C^TAG
MamI 594 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 563 TGGCCA TGG^CCA
MscI 563 TGGCCA TGG^CCA
MslI 177 CACCGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI 763 CACGTG CAC^GTG
PmlI 763 CACGTG CAC^GTG
PshAI 477 GACCGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 403 CAGCTG CAG^CTG
RsaI 309 GTAC GT^AC
RsrII 475 CGGACCG CG^G[AT]CCG
SacI 1309 GAGCTC GAGCT^C
SacII 218 CCGCGG CCGC^GG
SfiI 1009 GGCCTGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1049 CTCAAG C^T[CT][GA]AG
StyI 1068 CCAAGG C^C[AT][AT]GG
TaqI 615 TCGA T^CGA
Tsp509I 161 AATT ^AATT
TspRI 95 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 615 TCGA T^CGA
XcmI 326 CCAGAGAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccIII 357 TCCGGA T^CCGGA
921 TCCGGA
Alw44I 97 GTGCAC G^TGCAC
115 GTGCAC
ApaI 1017 GGGCCC GGGCC^C
1042 GGGCCC
ApaLI 97 GTGCAC G^TGCAC
115 GTGCAC
BseAI 357 TCCGGA T^CCGGA
921 TCCGGA
BsmBI 444 GAGACG CGTCTC(1/5)
571 GAGACG
BspEI 357 TCCGGA T^CCGGA
921 TCCGGA
BspMI 87 ACCTGC ACCTGC(4/8)
876 ACCTGC
BsrBI 1046 CCGCTC CCGCTC(-3/-3)
298 GAGCGG
BssHII 773 GCGCGC G^CGCGC
886 GCGCGC
BstF5I 1236 CATCC GGATG(2/0)
1305 GGATG
DsaI 218 CCGCGG C^C[GA][CT]GG
1105 CCGTGG
EaeI 166 CGGCCG [CT]^GGCC[GA]
563 TGGCCA
EcoNI 31 CCTTTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1416 CCTCAGCGAGG
FokI 1236 CATCC GGATG(9/13)
1305 GGATG
MaeII 764 ACGT A^CGT
1090 ACGT
MboII 1390 GAAGA GAAGA(8/7)
1426 GAAGA
MroI 357 TCCGGA T^CCGGA
921 TCCGGA
MspA1I 218 CCGCGG C[AC]G^C[GT]G
403 CAGCTG
PpuMI 992 GGGACCC [GA]G^G[AT]CC[CT]
1125 GGGTCCC
PstI 835 CTGCAG CTGCA^G
1333 CTGCAG
SfcI 835 CTGCAG C^T[GA][CT]AG
1333 CTGCAG
SgrAI 415 CACCGGCG C[GA]^CCGG[CT]G
729 CACCGGCG
StuI 72 AGGCCT AGG^CCT
1382 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbeI BbvCI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeIII MboI MflI
MnlI MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI SfoI SmaI TfiI TseI
Tsp45I XhoII XmaCI XmaI