(Circular) (Six-base) MAPSORT of: pFN21AE3815.seq Check: 7989 from: 1 to: 6160 pFN21AE3815 6160 bp With 182 enzymes: SgfI * July 24, 2018 19:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2101 2101 Size: 6160 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3503 1937 Size: 1566 4594 Acc65I G'GTAC_C Cuts at: 3481 3481 Size: 6160 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4223 5607 5875 1632 Size: 2591 1384 268 1917 Fragments arranged by size: 2591 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 5059 6036 1203 Size: 3856 977 1327 Fragments arranged by size: 3856 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3310 1184 Size: 2126 4034 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2740 2772 2740 Size: 32 6128 AlwNI CAG_nnn'CTG Cuts at: 3903 5475 3903 Size: 1572 4588 ApaI G_GGCC'C Cuts at: 3026 3051 3026 Size: 25 6135 ApaLI G'TGCA_C Cuts at: 2102 2120 4217 5373 2102 Size: 18 2097 1156 2889 Fragments arranged by size: 2889 2097 1156 18 ApoI r'AATT_y Cuts at: 1493 2165 3469 3695 3847 5956 1493 Size: 672 1304 226 152 2109 1697 Fragments arranged by size: 2109 1697 1304 672 226 152 AseI AT'TA_AT Cuts at: 160 1032 4714 160 Size: 872 3682 1606 Fragments arranged by size: 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 2232 2269 2317 2444 2556 2635 2974 Size: 73 289 37 48 127 112 79 339 Cuts at: 2974 3373 3485 1870 Size: 399 112 4545 Fragments arranged by size: 4545 399 339 289 127 112 112 79 73 48 37 BamHI G'GATC_C Cuts at: 2495 3490 3857 2495 Size: 995 367 4798 Fragments arranged by size: 4798 995 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2631 2653 3247 3274 3481 Size: 325 217 627 844 22 594 27 207 Cuts at: 3481 4936 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 844 627 594 325 217 207 27 22 BanII G_rGCy'C Cuts at: 1391 2132 2181 2370 2791 3026 3051 3318 3329 Size: 741 49 189 421 235 25 267 11 Cuts at: 3329 3445 3479 1391 Size: 116 34 4072 Fragments arranged by size: 4072 741 421 267 235 189 116 49 34 25 11 BbeI G_GCGC'C Cuts at: 2657 3251 3278 2657 Size: 594 27 5539 Fragments arranged by size: 5539 594 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5189 BbvCI CC'TCA_GC Cuts at: 2115 2411 3422 2115 Size: 296 1011 4853 Fragments arranged by size: 4853 1011 296 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4351 4385 1903 Size: 34 2414 34 3678 Fragments arranged by size: 3678 2414 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4052 5262 4052 Size: 1210 4950 BclI T'GATC_A Cuts at: 1548 1548 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2861 2918 3021 Size: 107 122 71 631 605 1189 57 103 Cuts at: 3021 3149 4771 136 Size: 128 1622 1525 Fragments arranged by size: 1622 1525 1189 631 605 128 122 107 103 71 57 BglII A'GATC_T Cuts at: 2604 6155 2604 Size: 3551 2609 BlpI GC'TnA_GC Cuts at: 3577 3577 Size: 6160 Bme1580I G_kGCm'C Cuts at: 1790 2106 2124 2775 3026 3051 4221 5377 1790 Size: 316 18 651 251 25 1170 1156 2573 Fragments arranged by size: 2573 1170 1156 651 316 251 25 18 BmgBI CAC'GTC Cuts at: 1318 3096 1318 Size: 1778 4382 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3891 4849 442 Size: 416 53 83 399 24 2474 958 1753 Fragments arranged by size: 2474 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2890 3421 2890 Size: 531 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3248 3404 3470 4820 1239 Size: 2009 156 66 1350 2579 Fragments arranged by size: 2579 2009 1350 156 66 Bpu10I CC'TnA_GC Cuts at: 2115 2411 3422 2115 Size: 296 1011 4853 Fragments arranged by size: 4853 1011 296 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3039 4285 5150 5448 3039 Size: 1246 865 298 3751 Fragments arranged by size: 3751 1246 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4823 882 Size: 617 3324 2219 Fragments arranged by size: 3324 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2770 3951 493 Size: 711 1566 1181 2702 Fragments arranged by size: 2702 1566 1181 711 BsaBI GATnn'nnATC Cuts at: 2603 6154 2603 Size: 3551 2609 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2654 3204 3248 3275 Size: 53 83 186 936 1121 550 44 27 Cuts at: 3275 3967 4349 4984 275 Size: 692 382 635 1451 Fragments arranged by size: 1451 1121 936 692 635 550 382 186 83 53 44 27 BsaWI w'CCGG_w Cuts at: 2362 2492 2926 4593 4991 5265 5412 5823 2362 Size: 130 434 1667 398 274 147 411 2699 Fragments arranged by size: 2699 1667 434 411 398 274 147 130 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5935 Fragments arranged by size: 5935 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2504 2576 2913 2946 3467 1186 Size: 1318 72 337 33 521 3879 Fragments arranged by size: 3879 1318 521 337 72 33 BseYI C'CCAG_C Cuts at: 2846 3120 3186 5363 5859 2846 Size: 274 66 2177 496 3147 Fragments arranged by size: 3147 2177 496 274 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2073 2698 3270 1163 Size: 573 337 625 572 4053 Fragments arranged by size: 4053 625 573 572 337 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2174 3610 4371 5399 1540 Size: 275 186 173 1436 761 1028 2301 Fragments arranged by size: 2301 1436 1028 761 275 186 173 BsiHKAI G_wGCw'C Cuts at: 2106 2124 3318 4221 4306 5377 2106 Size: 18 1194 903 85 1071 2889 Fragments arranged by size: 2889 1194 1071 903 85 18 BsmI GAATG_Cn' Cuts at: 2358 3671 3764 2358 Size: 1313 93 4754 Fragments arranged by size: 4754 1313 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2443 2570 2443 Size: 127 6033 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2124 2132 2181 2370 2775 2791 Size: 399 316 18 8 49 189 405 16 Cuts at: 2791 3026 3051 3318 3329 3445 3479 4221 4306 Size: 235 25 267 11 116 34 742 85 Cuts at: 4306 5377 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 405 399 316 267 235 189 116 85 49 34 25 18 16 11 8 BspEI T'CCGG_A Cuts at: 2362 2926 5823 2362 Size: 564 2897 2699 Fragments arranged by size: 2897 2699 564 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2101 2890 3515 844 Size: 1257 789 625 3489 Fragments arranged by size: 3489 1257 789 625 BsrBI CCG'CTC Cuts at: 1394 1823 2305 3053 4047 1394 Size: 429 482 748 994 3507 Fragments arranged by size: 3507 994 748 482 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4655 4829 65 Size: 1183 3407 174 1396 Fragments arranged by size: 3407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2134 2421 2469 2735 2860 3271 4804 Size: 110 324 287 48 266 125 411 1533 Cuts at: 4804 4991 1700 Size: 187 2869 Fragments arranged by size: 2869 1533 411 324 287 266 187 125 110 48 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6160 BssHII G'CGCG_C Cuts at: 1925 2778 2891 1925 Size: 853 113 5194 Fragments arranged by size: 5194 853 113 BssSI C'ACGA_G Cuts at: 2713 4220 5232 2713 Size: 1507 1012 3641 Fragments arranged by size: 3641 1507 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6160 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 2699 1184 Size: 1515 4645 BstYI r'GATC_y Cuts at: 1972 2495 2604 2871 3490 3857 4242 4259 5711 Size: 523 109 267 619 367 385 17 1452 Cuts at: 5711 5797 5809 6155 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 619 523 385 367 346 267 109 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2949 2949 Size: 6160 BtgI C'CryG_G Cuts at: 513 1065 2223 3110 513 Size: 552 1158 887 3563 Fragments arranged by size: 3563 1158 887 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2201 2308 2533 487 Size: 683 56 975 107 225 4114 Fragments arranged by size: 4114 975 683 225 107 56 BtsI GCAGTG_nn' Cuts at: 2106 3676 4470 4490 2106 Size: 1570 794 20 3776 Fragments arranged by size: 3776 1570 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6125 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2171 2568 3607 Size: 54 1425 50 275 18 341 397 1039 Cuts at: 3607 4496 6125 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1039 889 397 341 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2171 3607 1537 Size: 275 359 1436 4090 Fragments arranged by size: 4090 1436 359 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3390 4090 1353 Size: 2037 700 3423 Fragments arranged by size: 3423 2037 700 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3199 4747 5121 5267 387 Size: 1064 138 1610 1548 374 146 1280 Fragments arranged by size: 1610 1548 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3316 3316 Size: 6160 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3248 3404 3470 4223 4820 5607 5875 Size: 393 1616 156 66 753 597 787 268 Cuts at: 5875 1239 Size: 1524 Fragments arranged by size: 1616 1524 787 753 597 393 268 156 66 EcoNI CCTnn'n_nnAGG Cuts at: 2040 3425 2040 Size: 1385 4775 EcoO109I rG'GnC_Cy Cuts at: 2284 2998 3023 3131 3370 2284 Size: 714 25 108 239 5074 Fragments arranged by size: 5074 714 239 108 25 EcoRI G'AATT_C Cuts at: 3469 3847 3469 Size: 378 5782 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2554 3098 2554 Size: 544 5616 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 2657 2939 3251 3263 3278 5307 1678 Size: 979 282 312 12 15 2029 2531 Fragments arranged by size: 2531 2029 979 312 282 15 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 4801 4833 4875 Size: 32 135 32 669 32 2528 32 42 Cuts at: 4875 4907 1373 Size: 32 2626 Fragments arranged by size: 2626 2528 669 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3504 3750 669 Size: 988 281 1566 246 3079 Fragments arranged by size: 3079 1566 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6160 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KasI G'GCGC_C Cuts at: 2653 3247 3274 2653 Size: 594 27 5539 Fragments arranged by size: 5539 594 27 KpnI G_GTAC'C Cuts at: 3485 3485 Size: 6160 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5274 5458 1305 Size: 3969 184 2007 Fragments arranged by size: 3969 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2570 10 Size: 54 1425 343 738 3600 Fragments arranged by size: 3600 1425 738 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 4118 4477 4636 518 Size: 648 1020 1932 359 159 2042 Fragments arranged by size: 2042 1932 1020 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2225 2410 3576 4253 5401 5646 1606 Size: 619 185 1166 677 1148 245 2120 Fragments arranged by size: 2120 1166 1148 677 619 245 185 NaeI GCC'GGC Cuts at: 2136 2471 2862 3273 2136 Size: 335 391 411 5023 Fragments arranged by size: 5023 411 391 335 NarI GG'CG_CC Cuts at: 2654 3248 3275 2654 Size: 594 27 5539 Fragments arranged by size: 5539 594 27 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5608 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NgoMIV G'CCGG_C Cuts at: 2134 2469 2860 3271 2134 Size: 335 391 411 5023 Fragments arranged by size: 5023 411 391 335 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 3518 5063 1132 Size: 97 2289 1545 2229 Fragments arranged by size: 2289 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2370 3100 1348 Size: 1022 730 4408 Fragments arranged by size: 4408 1022 730 PciI A'CATG_T Cuts at: 5059 5059 Size: 6160 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6160 PfoI T'CCnGG_A Cuts at: 2586 2594 3264 2586 Size: 8 670 5482 Fragments arranged by size: 5482 670 8 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PmlI CAC'GTG Cuts at: 2770 2770 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4229 4261 5742 5774 4229 Size: 32 1481 32 4615 Fragments arranged by size: 4615 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2998 3131 2998 Size: 133 6027 PshAI GACnn'nnGTC Cuts at: 1613 2486 1613 Size: 873 5287 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PspOMI G'GGCC_C Cuts at: 3022 3047 3022 Size: 25 6135 PstI C_TGCA'G Cuts at: 830 2844 3342 3512 830 Size: 2014 498 170 3478 Fragments arranged by size: 3478 2014 498 170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 PvuII CAG'CTG Cuts at: 2410 2410 Size: 6160 RsrII CG'GwC_CG Cuts at: 2481 2481 Size: 6160 SacI G_AGCT'C Cuts at: 3318 3318 Size: 6160 SacII CC_GC'GG Cuts at: 2226 2226 Size: 6160 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SanDI GG'GwC_CC Cuts at: 2998 3131 2998 Size: 133 6027 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 4408 6120 4408 Size: 1712 4448 SfcI C'TryA_G Cuts at: 826 1045 2840 3338 3508 4643 5324 5515 826 Size: 219 1795 498 170 1135 681 191 1471 Fragments arranged by size: 1795 1471 1135 681 498 219 191 170 SfiI GGCCn_nnn'nGGCC Cuts at: 3021 3021 Size: 6160 SfoI GGC'GCC Cuts at: 2655 3249 3276 2655 Size: 594 27 5539 Fragments arranged by size: 5539 594 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 2421 2735 1700 Size: 721 314 5125 Fragments arranged by size: 5125 721 314 SmaI CCC'GGG Cuts at: 1872 2234 2319 2446 2558 2637 2976 3375 3487 Size: 362 85 127 112 79 339 399 112 Cuts at: 3487 1872 Size: 4545 Fragments arranged by size: 4545 399 362 339 127 112 112 85 79 SmlI C'TyrA_G Cuts at: 820 1017 1943 3054 4264 5165 5427 820 Size: 197 926 1111 1210 901 262 1553 Fragments arranged by size: 1553 1210 1111 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 3518 1132 Size: 2386 3774 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 2079 3389 1517 Size: 562 1310 4288 Fragments arranged by size: 4288 1310 562 StyI C'CwwG_G Cuts at: 513 1065 3073 3599 513 Size: 552 2008 526 3074 Fragments arranged by size: 3074 2008 552 526 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3106 4187 4204 4357 4542 4987 325 Size: 1255 1526 1081 17 153 185 445 1498 Fragments arranged by size: 1526 1498 1255 1081 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4406 6118 96 Size: 274 80 33 51 1443 2429 1712 138 Fragments arranged by size: 2429 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6120 6152 6120 Size: 32 6128 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6160 XbaI T'CTAG_A Cuts at: 3496 3496 Size: 6160 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2338 Size: 6160 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 2232 2317 2444 2556 2635 2974 3373 3485 Size: 362 85 127 112 79 339 399 112 Cuts at: 3485 1870 Size: 4545 Fragments arranged by size: 4545 399 362 339 127 112 112 85 79 XmnI GAAnn'nnTTC Cuts at: 4289 4289 Size: 6160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI SapI SexAI SrfI SwaI