Restriction map for
pFN21AE3815
(Linear) (Six-base) MAPSORT of: KIEE3815.seq Check: 7558 from: 1 to: 1455
KIEE3815.orf
With 182 enzymes: SgfI *
July 24, 2018 16:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 96 1455
Size: 96 1359
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1305 1455
Size: 1305 150
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 735 767 1455
Size: 735 32 688
Fragments arranged by size:
735 688 32
ApaI G_GGCC'C
Cuts at: 0 1021 1046 1455
Size: 1021 25 409
Fragments arranged by size:
1021 409 25
ApaLI G'TGCA_C
Cuts at: 0 97 115 1455
Size: 97 18 1340
Fragments arranged by size:
1340 97 18
ApoI r'AATT_y
Cuts at: 0 160 1455
Size: 160 1295
AvaI C'yCGr_G
Cuts at: 0 227 264 312 439 551 630 969 1368
Size: 227 37 48 127 112 79 339 399
Cuts at: 1368 1455
Size: 87
Fragments arranged by size:
399 339 227 127 112 87 79 48
37
BamHI G'GATC_C
Cuts at: 0 490 1455
Size: 490 965
BanI G'GyrC_C
Cuts at: 0 626 648 1242 1269 1455
Size: 626 22 594 27 186
Fragments arranged by size:
626 594 186 27 22
BanII G_rGCy'C
Cuts at: 0 127 176 365 786 1021 1046 1313 1324
Size: 127 49 189 421 235 25 267 11
Cuts at: 1324 1440 1455
Size: 116 15
Fragments arranged by size:
421 267 235 189 127 116 49 25
15 11
BbeI G_GCGC'C
Cuts at: 0 652 1246 1273 1455
Size: 652 594 27 182
Fragments arranged by size:
652 594 182 27
BbvCI CC'TCA_GC
Cuts at: 0 110 406 1417 1455
Size: 110 296 1011 38
Fragments arranged by size:
1011 296 110 38
BglI GCCn_nnn'nGGC
Cuts at: 0 856 913 1016 1144 1455
Size: 856 57 103 128 311
Fragments arranged by size:
856 311 128 103 57
BglII A'GATC_T
Cuts at: 0 599 1455
Size: 599 856
Bme1580I G_kGCm'C
Cuts at: 0 101 119 770 1021 1046 1455
Size: 101 18 651 251 25 409
Fragments arranged by size:
651 409 251 101 25 18
BmgBI CAC'GTC
Cuts at: 0 1091 1455
Size: 1091 364
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 885 1416 1455
Size: 885 531 39
Fragments arranged by size:
885 531 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1399 1455
Size: 1243 156 56
Fragments arranged by size:
1243 156 56
Bpu10I CC'TnA_GC
Cuts at: 0 110 406 1417 1455
Size: 110 296 1011 38
Fragments arranged by size:
1011 296 110 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1455
Size: 1034 421
BsaAI yAC'GTr
Cuts at: 0 765 1455
Size: 765 690
BsaBI GATnn'nnATC
Cuts at: 0 598 1455
Size: 598 857
BsaHI Gr'CG_yC
Cuts at: 0 649 1199 1243 1270 1455
Size: 649 550 44 27 185
Fragments arranged by size:
649 550 185 44 27
BsaWI w'CCGG_w
Cuts at: 0 357 487 921 1455
Size: 357 130 434 534
Fragments arranged by size:
534 434 357 130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 499 571 908 941 1455
Size: 499 72 337 33 514
Fragments arranged by size:
514 499 337 72 33
BseYI C'CCAG_C
Cuts at: 0 841 1115 1181 1455
Size: 841 274 66 274
Fragments arranged by size:
841 274 274 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 693 1265 1455
Size: 68 625 572 190
Fragments arranged by size:
625 572 190 68
BsiEI CG_ry'CG
Cuts at: 0 169 1455
Size: 169 1286
BsiHKAI G_wGCw'C
Cuts at: 0 101 119 1313 1455
Size: 101 18 1194 142
Fragments arranged by size:
1194 142 101 18
BsmI GAATG_Cn'
Cuts at: 0 353 1455
Size: 353 1102
BsmBI CGTCTCn'nnnn_
Cuts at: 0 438 565 1455
Size: 438 127 890
Fragments arranged by size:
890 438 127
Bsp1286I G_dGCh'C
Cuts at: 0 101 119 127 176 365 770 786 1021
Size: 101 18 8 49 189 405 16 235
Cuts at: 1021 1046 1313 1324 1440 1455
Size: 25 267 11 116 15
Fragments arranged by size:
405 267 235 189 116 101 49 25
18 16 15 11 8
BspEI T'CCGG_A
Cuts at: 0 357 921 1455
Size: 357 564 534
Fragments arranged by size:
564 534 357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 96 885 1455
Size: 96 789 570
Fragments arranged by size:
789 570 96
BsrBI CCG'CTC
Cuts at: 0 300 1048 1455
Size: 300 748 407
Fragments arranged by size:
748 407 300
BsrFI r'CCGG_y
Cuts at: 0 129 416 464 730 855 1266 1455
Size: 129 287 48 266 125 411 189
Fragments arranged by size:
411 287 266 189 129 125 48
BssHII G'CGCG_C
Cuts at: 0 773 886 1455
Size: 773 113 569
Fragments arranged by size:
773 569 113
BssSI C'ACGA_G
Cuts at: 0 708 1455
Size: 708 747
BstEII G'GTnAC_C
Cuts at: 0 694 1455
Size: 694 761
BstYI r'GATC_y
Cuts at: 0 490 599 866 1455
Size: 490 109 267 589
Fragments arranged by size:
589 490 267 109
Bsu36I CC'TnA_GG
Cuts at: 0 944 1455
Size: 944 511
BtgI C'CryG_G
Cuts at: 0 218 1105 1455
Size: 218 887 350
Fragments arranged by size:
887 350 218
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 196 303 528 1455
Size: 196 107 225 927
Fragments arranged by size:
927 225 196 107
BtsI GCAGTG_nn'
Cuts at: 0 101 1455
Size: 101 1354
EaeI y'GGCC_r
Cuts at: 0 166 563 1455
Size: 166 397 892
Fragments arranged by size:
892 397 166
EagI C'GGCC_G
Cuts at: 0 166 1455
Size: 166 1289
EarI CTCTTCn'nnn_
Cuts at: 0 1385 1455
Size: 1385 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1194 1455
Size: 1194 261
EcoICRI GAG'CTC
Cuts at: 0 1311 1455
Size: 1311 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1399 1455
Size: 1243 156 56
Fragments arranged by size:
1243 156 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 35 1420 1455
Size: 35 1385 35
Fragments arranged by size:
1385 35 35
EcoO109I rG'GnC_Cy
Cuts at: 0 279 993 1018 1126 1365 1455
Size: 279 714 25 108 239 90
Fragments arranged by size:
714 279 239 108 90 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 549 1093 1455
Size: 549 544 362
Fragments arranged by size:
549 544 362
HaeII r_GCGC'y
Cuts at: 0 652 934 1246 1258 1273 1455
Size: 652 282 312 12 15 182
Fragments arranged by size:
652 312 282 182 15 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 1455
Size: 236 32 1187
Fragments arranged by size:
1187 236 32
KasI G'GCGC_C
Cuts at: 0 648 1242 1269 1455
Size: 648 594 27 186
Fragments arranged by size:
648 594 186 27
MscI TGG'CCA
Cuts at: 0 565 1455
Size: 565 890
MslI CAynn'nnrTG
Cuts at: 0 181 1455
Size: 181 1274
MspA1I CmG'CkG
Cuts at: 0 220 405 1455
Size: 220 185 1050
Fragments arranged by size:
1050 220 185
NaeI GCC'GGC
Cuts at: 0 131 466 857 1268 1455
Size: 131 335 391 411 187
Fragments arranged by size:
411 391 335 187 131
NarI GG'CG_CC
Cuts at: 0 649 1243 1270 1455
Size: 649 594 27 185
Fragments arranged by size:
649 594 185 27
NgoMIV G'CCGG_C
Cuts at: 0 129 464 855 1266 1455
Size: 129 335 391 411 189
Fragments arranged by size:
411 391 335 189 129
PasI CC'CwG_GG
Cuts at: 0 365 1095 1455
Size: 365 730 360
Fragments arranged by size:
730 365 360
PfoI T'CCnGG_A
Cuts at: 0 581 589 1259 1455
Size: 581 8 670 196
Fragments arranged by size:
670 581 196 8
PmlI CAC'GTG
Cuts at: 0 765 1455
Size: 765 690
PpuMI rG'GwC_Cy
Cuts at: 0 993 1126 1455
Size: 993 133 329
Fragments arranged by size:
993 329 133
PshAI GACnn'nnGTC
Cuts at: 0 481 1455
Size: 481 974
PspOMI G'GGCC_C
Cuts at: 0 1017 1042 1455
Size: 1017 25 413
Fragments arranged by size:
1017 413 25
PstI C_TGCA'G
Cuts at: 0 839 1337 1455
Size: 839 498 118
Fragments arranged by size:
839 498 118
PvuII CAG'CTG
Cuts at: 0 405 1455
Size: 405 1050
RsrII CG'GwC_CG
Cuts at: 0 476 1455
Size: 476 979
SacI G_AGCT'C
Cuts at: 0 1313 1455
Size: 1313 142
SacII CC_GC'GG
Cuts at: 0 221 1455
Size: 221 1234
SanDI GG'GwC_CC
Cuts at: 0 993 1126 1455
Size: 993 133 329
Fragments arranged by size:
993 329 133
SfcI C'TryA_G
Cuts at: 0 835 1333 1455
Size: 835 498 122
Fragments arranged by size:
835 498 122
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1016 1455
Size: 1016 439
SfoI GGC'GCC
Cuts at: 0 650 1244 1271 1455
Size: 650 594 27 184
Fragments arranged by size:
650 594 184 27
SgrAI Cr'CCGG_yG
Cuts at: 0 416 730 1455
Size: 416 314 725
Fragments arranged by size:
725 416 314
SmaI CCC'GGG
Cuts at: 0 229 314 441 553 632 971 1370 1455
Size: 229 85 127 112 79 339 399 85
Fragments arranged by size:
399 339 229 127 112 85 85 79
SmlI C'TyrA_G
Cuts at: 0 1049 1455
Size: 1049 406
StuI AGG'CCT
Cuts at: 0 74 1384 1455
Size: 74 1310 71
Fragments arranged by size:
1310 74 71
StyI C'CwwG_G
Cuts at: 0 1068 1455
Size: 1068 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1101 1455
Size: 1101 354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 1455
Size: 333 1122
XmaI C'CCGG_G
Cuts at: 0 227 312 439 551 630 969 1368 1455
Size: 227 85 127 112 79 339 399 87
Fragments arranged by size:
399 339 227 127 112 87 85 79
Enzymes that do cut:
AarI AlfI AloI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbvCI BglI BglII Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssHII BssSI BstEII BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI
MscI MslI MspA1I NaeI NarI NgoMIV PasI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuII RsrII SacI
SacII SanDI SfcI SfiI SfoI SgrAI SmaI SmlI
StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlwNI AscI AseI AsiSI AvrII
BaeI BbsI BcgI BciVI BclI BfrBI BlpI BmrI
BmtI BsaI BsaXI BsiWI BspHI BsrDI BsrGI BstAPI
BstBI BstXI BstZ17I ClaI CspCI DraI DraIII DrdI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PpiI
PsiI PspXI PsrI PvuI SalI SapI SbfI ScaI
SexAI SgfI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmnI ZraI