################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AclI AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BciVI BclI BfaI BlnI BlpI Bpu1102I BsaAI BsaBI BsiWI BsmBI BsmFI Bsp1407I BspDI BspHI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KasI KpnI KspI MaeI MamI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SfiI SfoI SfuI SnaBI SpeI SphI SspBI SspI SwaI Van91I XbaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 66 CGCG CG^CG AcyI 96 GACGCC G[GA]^CG[CT]C AfaI 90 GTAC GT^AC AflII 1690 CTTAAG C^TTAAG AflIII 440 ACATGT A^C[GA][CT]GT Alw44I 969 GTGCAC G^TGCAC Aor51HI 1099 AGCGCT AGC^GCT ApaLI 969 GTGCAC G^TGCAC AspHI 969 GTGCAC G[AT]GC[AT]^C AspI 295 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 18 GGGCTC G[GA]GC[CT]^C BbvCI 1371 GCTGAGG CCTCAGC(-5/-2) BcgI 173 GCAAAACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1690 CTTAAG C^TTAAG BglI 1088 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 926 AGATCT A^GATCT BsaHI 96 GACGCC G[GA]^CG[CT]C BsaI 666 GGTCTC GGTCTC(1/5) BseRI 585 GAGGAG GAGGAG(10/8) BsiEI 1068 CGGTCG CG[GA][CT]^CG BsiHKAI 969 GTGCAC G[AT]GC[AT]^C BsmAI 667 GTCTC GTCTC(1/5) BspLU11I 440 ACATGT A^CATGT BspMI 54 GCAGGT ACCTGC(4/8) BsrBI 641 GAGCGG CCGCTC(-3/-3) BsrDI 1040 GCAATG GCAATG(2/0) BsrFI 99 GCCGGT [GA]^CCGG[CT] BssHII 65 GCGCGC G^CGCGC BstUI 66 CGCG CG^CG BstXI 1226 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 99 GCCGGT [GA]^CCGG[CT] DraI 1456 TTTAAA TTT^AAA DraII 299 AGGTCCT [GA]G^G[ACGT]CC[CT] Eco47III 1099 AGCGCT AGC^GCT EcoO109I 299 AGGTCCT [GA]G^G[ACGT]CC[CT] HaeII 1099 AGCGCT [GA]GCGC^[CT] Hin1I 96 GACGCC G[GA]^CG[CT]C MaeII 1433 ACGT A^CGT MvnI 66 CGCG CG^CG NdeI 1280 CATATG CA^TATG PflFI 295 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 299 AGGTCCT [GA]G^G[AT]CC[CT] RsaI 90 GTAC GT^AC SbfI 1170 CCTGCAGG CCTGCA^GG SgrAI 98 CGCCGGTG C[GA]^CCGG[CT]G SmaI 986 CCCGGG CCC^GGG Sse8387I 1170 CCTGCAGG CCTGCA^GG StuI 594 AGGCCT AGG^CCT Tth111I 295 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 269 CCACTCGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 986 CCCGGG C^CCGGG XmaI 986 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccIII 446 TCCGGA T^CCGGA 650 TCCGGA AlwNI 55 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 822 CAGCACCTG BbsI 708 GAAGAC GAAGAC(2/6) 814 GTCTTC BpuAI 708 GAAGAC GAAGAC(2/6) 814 GTCTTC BsaWI 446 TCCGGA [AT]^CCGG[AT] 650 TCCGGA BseAI 446 TCCGGA T^CCGGA 650 TCCGGA BsgI 46 CTGCAC GTGCAG(16/14) 103 GTGCAG BsmI 1306 GAATGC GAATGC(1/-1) 893 GCATTC BspEI 446 TCCGGA T^CCGGA 650 TCCGGA BsrI 1013 ACTGG ACTGG(1/-1) 1741 ACTGG EcoT14I 1121 CCATGG C^C[AT][AT]GG 1226 CCATGG HgaI 96 GACGC GACGC(5/10) 615 GACGC HincII 1009 GTCAAC GT[CT]^[GA]AC 1734 GTTGAC HindII 1009 GTCAAC GT[CT]^[GA]AC 1734 GTTGAC MaeIII 10 GTGAC ^GT[ACGT]AC 160 GTCAC MroI 446 TCCGGA T^CCGGA 650 TCCGGA MslI 408 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 972 CACTGATGTG NcoI 1121 CCATGG C^CATGG 1226 CCATGG NspI 6 GCATGT [GA]CATG^[CT] 440 ACATGT PaeR7I 272 CTCGAG C^TCGAG 1355 CTCGAG PstI 149 CTGCAG CTGCA^G 1171 CTGCAG PvuII 477 CAGCTG CAG^CTG 769 CAGCTG SexAI 632 ACCTGGT A^CC[AT]GGT 809 ACCTGGT SfcI 149 CTGCAG C^T[GA][CT]AG 1171 CTGCAG StyI 1121 CCATGG C^C[AT][AT]GG 1226 CCATGG TaqI 273 TCGA T^CGA 1356 TCGA Tsp45I 10 GTGAC ^GT[GC]AC 160 GTCAC TthHB8I 273 TCGA T^CGA 1356 TCGA XhoI 272 CTCGAG C^TCGAG 1355 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI Asp700I AvaI AvaII BalI BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SmlI TfiI Tru9I TseI Tsp509I TspRI XhoII XmnI