Restriction map for
pFN21AE5770
(Circular) (Six-base) MAPSORT of: pFN21AE5770.seq Check: 1838 from: 1 to: 6505
pFN21AE5770 6505 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2050 2050
Size: 6505
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4315 278
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3848 1937
Size: 1911 4594
Acc65I G'GTAC_C
Cuts at: 3826 3826
Size: 6505
AclI AA'CG_TT
Cuts at: 1563 4632 5005 1563
Size: 3069 373 3063
Fragments arranged by size:
3069 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2809 3030 3735 4568 5952 6220 1632
Size: 1177 221 705 833 1384 268 1917
Fragments arranged by size:
1917 1384 1177 833 705 268 221
AfeI AGC'GCT
Cuts at: 3106 3106
Size: 6505
AflII C'TTAA_G
Cuts at: 820 1017 3695 820
Size: 197 2678 3630
Fragments arranged by size:
3630 2678 197
AflIII A'CryG_T
Cuts at: 1203 2445 5404 6381 1203
Size: 1242 2959 977 1327
Fragments arranged by size:
2959 1327 1242 977
AgeI A'CCGG_T
Cuts at: 5336 5336
Size: 6505
AhdI GACnn_n'nnGTC
Cuts at: 5234 5234
Size: 6505
AleI CACnn'nnGTG
Cuts at: 1166 2981 1166
Size: 1815 4690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6505
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2432 2464 2432
Size: 32 6473
AlwNI CAG_nnn'CTG
Cuts at: 2065 2832 4248 5820 2065
Size: 767 1416 1572 2750
Fragments arranged by size:
2750 1572 1416 767
ApaLI G'TGCA_C
Cuts at: 2974 4562 5718 2974
Size: 1588 1156 3761
Fragments arranged by size:
3761 1588 1156
ApoI r'AATT_y
Cuts at: 1493 2211 2765 3545 3722 3814 4040 4192 6301
Size: 718 554 780 177 92 226 152 2109
Cuts at: 6301 1493
Size: 1697
Fragments arranged by size:
2109 1697 780 718 554 226 177 152
92
AseI AT'TA_AT
Cuts at: 160 1032 5059 160
Size: 872 4027 1606
Fragments arranged by size:
4027 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
AvaI C'yCGr_G
Cuts at: 1870 1943 2277 2333 2991 3360 3830 1870
Size: 73 334 56 658 369 470 4545
Fragments arranged by size:
4545 658 470 369 334 73 56
BamHI G'GATC_C
Cuts at: 3835 4202 3835
Size: 367 6138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3826 5281 618
Size: 325 217 627 2039 1455 1842
Fragments arranged by size:
2039 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2027 3824 1391
Size: 636 1797 4072
Fragments arranged by size:
4072 1797 636
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2720 2812 928
Size: 971 821 92 4621
Fragments arranged by size:
4621 971 821 92
BbvCI CC'TCA_GC
Cuts at: 3377 3377
Size: 6505
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2167 2201 4696 4730 1903
Size: 34 230 34 2495 34 3678
Fragments arranged by size:
3678 2495 230 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4397 5607 4397
Size: 1210 5295
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6505
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3099 5116 136
Size: 107 122 71 631 605 1427 2017 1525
Fragments arranged by size:
2017 1525 1427 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2931 6500 2931
Size: 3569 2936
BlpI GC'TnA_GC
Cuts at: 3922 3922
Size: 6505
Bme1580I G_kGCm'C
Cuts at: 1790 2710 2978 3432 4566 5722 1790
Size: 920 268 454 1134 1156 2573
Fragments arranged by size:
2573 1156 1134 920 454 268
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6505
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3028 4236 5194
Size: 416 53 83 399 24 1611 1208 958
Cuts at: 5194 442
Size: 1753
Fragments arranged by size:
1753 1611 1208 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6505
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2669 2669
Size: 6505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2394 2412 2591 3521 5165 1239
Size: 1155 18 179 930 1644 2579
Fragments arranged by size:
2579 1644 1155 930 179 18
Bpu10I CC'TnA_GC
Cuts at: 3377 3377
Size: 6505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3209 4630 5495 5793 3209
Size: 1421 865 298 3921
Fragments arranged by size:
3921 1421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2677 5168 882
Size: 617 1178 2491 2219
Fragments arranged by size:
2491 2219 1178 617
BsaAI yAC'GTr
Cuts at: 493 1204 4296 493
Size: 711 3092 2702
Fragments arranged by size:
3092 2702 711
BsaBI GATnn'nnATC
Cuts at: 6499 6499
Size: 6505
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 4312 4694 5329
Size: 53 83 186 936 569 2210 382 635
Cuts at: 5329 275
Size: 1451
Fragments arranged by size:
2210 1451 936 635 569 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2451 2655 4938 5336 5610 5757 6168 2451
Size: 204 2283 398 274 147 411 2788
Fragments arranged by size:
2788 2283 411 398 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2367 2397 2432 2462 1178
Size: 30 165 30 964 30 35 30 5221
Fragments arranged by size:
5221 964 165 35 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2605 1186
Size: 1419 5086
BseYI C'CCAG_C
Cuts at: 2065 2083 3041 5708 6204 2065
Size: 18 958 2667 496 2366
Fragments arranged by size:
2667 2366 958 496 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 2129 1163
Size: 573 300 93 5539
Fragments arranged by size:
5539 573 300 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3076 3955 4716 5744 1540
Size: 275 186 1075 879 761 1028 2301
Fragments arranged by size:
2301 1075 1028 879 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2978 4566 4651 5722 2978
Size: 1588 85 1071 3761
Fragments arranged by size:
3761 1588 1071 85
BsmI GAATG_Cn'
Cuts at: 2898 3317 4016 4109 2898
Size: 419 699 93 5294
Fragments arranged by size:
5294 699 419 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2710 2978 3432 3824 4566 4651
Size: 399 237 683 268 454 392 742 85
Cuts at: 4651 5722 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 683 454 399 392 268
237 85
BspEI T'CCGG_A
Cuts at: 2451 2655 6168 2451
Size: 204 3513 2788
Fragments arranged by size:
3513 2788 204
BspHI T'CATG_A
Cuts at: 4394 6124 4394
Size: 1730 4775
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2050 3860 844
Size: 1206 1810 3489
Fragments arranged by size:
3489 1810 1206
BsrBI CCG'CTC
Cuts at: 1394 1823 2648 4392 1394
Size: 429 825 1744 3507
Fragments arranged by size:
3507 1744 825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3052 5000 5174 65
Size: 1183 1804 1948 174 1396
Fragments arranged by size:
1948 1804 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2104 5149 5336 1700
Size: 110 294 3045 187 2869
Fragments arranged by size:
3045 2869 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6505
BssHII G'CGCG_C
Cuts at: 1925 2070 1925
Size: 145 6360
BssSI C'ACGA_G
Cuts at: 4565 5577 4565
Size: 1012 5493
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6505
BstBI TT'CG_AA
Cuts at: 5309 5309
Size: 6505
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6505
BstXI CCAn_nnnn'nTGG
Cuts at: 3238 3238
Size: 6505
BstYI r'GATC_y
Cuts at: 1972 2473 2931 3337 3780 3835 4202 4587 4604
Size: 501 458 406 443 55 367 385 17
Cuts at: 4604 6056 6142 6154 6500 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 501 458 443 406 385 367
346 86 55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3094 3126 3231 3432 513
Size: 552 939 1090 32 105 201 3586
Fragments arranged by size:
3586 1090 939 552 201 105 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3050 487
Size: 683 56 1824 3942
Fragments arranged by size:
3942 1824 683 56
BtsI GCAGTG_nn'
Cuts at: 2710 4021 4815 4835 2710
Size: 1311 794 20 4380
Fragments arranged by size:
4380 1311 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6505
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6470
DraI TTT'AAA
Cuts at: 3463 3809 3463
Size: 346 6159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5512 809
Size: 507 4196 1802
Fragments arranged by size:
4196 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 2421 2478
Size: 54 1425 50 275 18 456 135 57
Cuts at: 2478 3091 3952 4841 6470 8
Size: 613 861 889 1629 43
Fragments arranged by size:
1629 1425 889 861 613 456 275 135
57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3952 1537
Size: 275 2140 4090
Fragments arranged by size:
4090 2140 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2218 2223 3126 3508 3621 4435 1353
Size: 865 5 903 382 113 814 3423
Fragments arranged by size:
3423 903 865 814 382 113 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5092 5466 5612 387
Size: 1064 138 3503 374 146 1280
Fragments arranged by size:
3503 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2394 2412 2591 2809 3030 3521 3735
Size: 393 762 18 179 218 221 491 214
Cuts at: 3735 4568 5165 5952 6220 1239
Size: 833 597 787 268 1524
Fragments arranged by size:
1524 833 787 762 597 491 393 268
221 218 214 179 18
EcoO109I rG'GnC_Cy
Cuts at: 2305 2305
Size: 6505
EcoRI G'AATT_C
Cuts at: 3814 4192 3814
Size: 378 6127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3683 3683
Size: 6505
FspI TGC'GCA
Cuts at: 5011 5011
Size: 6505
HaeII r_GCGC'y
Cuts at: 1678 3108 5652 1678
Size: 1430 2544 2531
Fragments arranged by size:
2544 2531 1430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2247 2279 2294 2326 5146
Size: 32 135 32 675 32 15 32 2820
Cuts at: 5146 5178 5220 5252 1373
Size: 32 42 32 2626
Fragments arranged by size:
2820 2626 675 135 42 32 32 32
32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3016 3741 3849 4095 669
Size: 988 281 1078 725 108 246 3079
Fragments arranged by size:
3079 1078 988 725 281 246 108
HindIII A'AGCT_T
Cuts at: 748 3273 3294 3561 748
Size: 2525 21 267 3692
Fragments arranged by size:
3692 2525 267 21
HpaI GTT'AAC
Cuts at: 4095 4095
Size: 6505
KpnI G_GTAC'C
Cuts at: 3830 3830
Size: 6505
MfeI C'AATT_G
Cuts at: 4104 4104
Size: 6505
MluI A'CGCG_T
Cuts at: 6381 6381
Size: 6505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2233 2493 5619 5803 1305
Size: 928 260 3126 184 2007
Fragments arranged by size:
3126 2007 928 260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2288 2423 2480 3093 10
Size: 54 1425 343 456 135 57 613 3422
Fragments arranged by size:
3422 1425 613 456 343 135 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 2981 4463 4822 4981 518
Size: 648 1251 564 1482 359 159 2042
Fragments arranged by size:
2042 1482 1251 648 564 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2484 2776 3206 3921 4598 5746 5991
Size: 444 434 292 430 715 677 1148 245
Cuts at: 5991 1606
Size: 2120
Fragments arranged by size:
2120 1148 715 677 444 434 430 292
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3126 3231 513
Size: 552 939 1122 105 3787
Fragments arranged by size:
3787 1122 939 552 105
NdeI CA'TA_TG
Cuts at: 387 3286 387
Size: 2899 3606
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6505
NotI GC'GGCC_GC
Cuts at: 3952 3952
Size: 6505
NspI r_CATG'y
Cuts at: 1132 1229 2015 2449 3863 5408 1132
Size: 97 786 434 1414 1545 2229
Fragments arranged by size:
2229 1545 1414 786 434 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6505
PciI A'CATG_T
Cuts at: 2445 5404 2445
Size: 2959 3546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6505
PfoI T'CCnGG_A
Cuts at: 2205 2371 3498 2205
Size: 166 1127 5212
Fragments arranged by size:
5212 1127 166
PmeI GTTT'AAAC
Cuts at: 3809 3809
Size: 6505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2432 2464 4574 4606 6087 6119 2432
Size: 32 2110 32 1481 32 2818
Fragments arranged by size:
2818 2110 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2305
Size: 6505
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6505
PsiI TTA'TAA
Cuts at: 4075 4075
Size: 6505
PspXI vC'TCGA_Gb
Cuts at: 3360 3360
Size: 6505
PstI C_TGCA'G
Cuts at: 830 2158 3180 3857 830
Size: 1328 1022 677 3478
Fragments arranged by size:
3478 1328 1022 677
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6505
PvuII CAG'CTG
Cuts at: 2484 2776 2484
Size: 292 6213
SalI G'TCGA_C
Cuts at: 1936 3847 1936
Size: 1911 4594
SbfI CC_TGCA'GG
Cuts at: 3180 3857 3180
Size: 677 5828
ScaI AGT'ACT
Cuts at: 4753 6465 4753
Size: 1712 4793
SexAI A'CCwGG_T
Cuts at: 2637 2814 2637
Size: 177 6328
SfcI C'TryA_G
Cuts at: 826 1045 2154 3176 3853 4988 5669 5860 826
Size: 219 1109 1022 677 1135 681 191 1471
Fragments arranged by size:
1471 1135 1109 1022 681 677 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
SgrAI Cr'CCGG_yG
Cuts at: 1700 2104 1700
Size: 404 6101
SmaI CCC'GGG
Cuts at: 1872 2993 3832 1872
Size: 1121 839 4545
Fragments arranged by size:
4545 1121 839
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2277 3224 3360 3695 4609 5510
Size: 197 926 334 947 136 335 914 901
Cuts at: 5510 5772 820
Size: 262 1553
Fragments arranged by size:
1553 947 926 914 901 335 334 262
197 136
SnaBI TAC'GTA
Cuts at: 493 4296 493
Size: 3803 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6505
SphI G_CATG'C
Cuts at: 1132 3863 1132
Size: 2731 3774
SspI AAT'ATT
Cuts at: 5 52 4429 5
Size: 47 4377 2081
Fragments arranged by size:
4377 2081 47
StuI AGG'CCT
Cuts at: 1517 2601 1517
Size: 1084 5421
StyI C'CwwG_G
Cuts at: 513 1065 2004 3126 3231 3944 513
Size: 552 939 1122 105 713 3074
Fragments arranged by size:
3074 1122 939 713 552 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4532 4549 4702 4887 5332 325
Size: 1255 2952 17 153 185 445 1498
Fragments arranged by size:
2952 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4751 6463 96
Size: 274 80 33 51 1443 2774 1712 138
Fragments arranged by size:
2774 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6465 6497 6465
Size: 32 6473
Tth111I GACn'n_nGTC
Cuts at: 1316 2303 1316
Size: 987 5518
XbaI T'CTAG_A
Cuts at: 3841 3841
Size: 6505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 2281
Size: 6505
XhoI C'TCGA_G
Cuts at: 1943 2277 3360 1943
Size: 334 1083 5088
Fragments arranged by size:
5088 1083 334
XmaI C'CCGG_G
Cuts at: 1870 2991 3830 1870
Size: 1121 839 4545
Fragments arranged by size:
4545 1121 839
XmnI GAAnn'nnTTC
Cuts at: 2214 2327 3663 4634 2214
Size: 113 1336 971 4085
Fragments arranged by size:
4085 1336 971 113
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4313 276
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI