(Linear) (Six-base) MAPSORT of: KIEE5770.seq Check: 3771 from: 1 to: 1800 KIEE5770.orf With 182 enzymes: SgfI * December 5, 2014 13:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 45 1800 Size: 45 1755 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 804 1025 1730 1800 Size: 804 221 705 70 Fragments arranged by size: 804 705 221 70 AfeI AGC'GCT Cuts at: 0 1101 1800 Size: 1101 699 AflII C'TTAA_G Cuts at: 0 1690 1800 Size: 1690 110 AflIII A'CryG_T Cuts at: 0 440 1800 Size: 440 1360 AleI CACnn'nnGTG Cuts at: 0 976 1800 Size: 976 824 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 427 459 1800 Size: 427 32 1341 Fragments arranged by size: 1341 427 32 AlwNI CAG_nnn'CTG Cuts at: 0 60 827 1800 Size: 60 767 973 Fragments arranged by size: 973 767 60 ApaLI G'TGCA_C Cuts at: 0 969 1800 Size: 969 831 ApoI r'AATT_y Cuts at: 0 206 760 1540 1717 1800 Size: 206 554 780 177 83 Fragments arranged by size: 780 554 206 177 83 AvaI C'yCGr_G Cuts at: 0 272 328 986 1355 1800 Size: 272 56 658 369 445 Fragments arranged by size: 658 445 369 272 56 BanII G_rGCy'C Cuts at: 0 22 1800 Size: 22 1778 BbsI GAAGACnn'nnnn_ Cuts at: 0 715 807 1800 Size: 715 92 993 Fragments arranged by size: 993 715 92 BbvCI CC'TCA_GC Cuts at: 0 1372 1800 Size: 1372 428 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 162 196 1800 Size: 162 34 1604 Fragments arranged by size: 1604 162 34 BglI GCCn_nnn'nGGC Cuts at: 0 1094 1800 Size: 1094 706 BglII A'GATC_T Cuts at: 0 926 1800 Size: 926 874 Bme1580I G_kGCm'C Cuts at: 0 705 973 1427 1800 Size: 705 268 454 373 Fragments arranged by size: 705 454 373 268 BmrI ACTGGGnnnn_n' Cuts at: 0 1023 1800 Size: 1023 777 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 664 1800 Size: 664 1136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 389 407 586 1516 1800 Size: 389 18 179 930 284 Fragments arranged by size: 930 389 284 179 18 Bpu10I CC'TnA_GC Cuts at: 0 1372 1800 Size: 1372 428 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1204 1800 Size: 1204 596 BsaI GGTCTCn'nnnn_ Cuts at: 0 672 1800 Size: 672 1128 BsaHI Gr'CG_yC Cuts at: 0 97 1800 Size: 97 1703 BsaWI w'CCGG_w Cuts at: 0 446 650 1800 Size: 446 204 1150 Fragments arranged by size: 1150 446 204 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 362 392 427 457 1800 Size: 362 30 35 30 1343 Fragments arranged by size: 1343 362 35 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 600 1800 Size: 600 1200 BseYI C'CCAG_C Cuts at: 0 60 78 1036 1800 Size: 60 18 958 764 Fragments arranged by size: 958 764 60 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 124 1800 Size: 31 93 1676 Fragments arranged by size: 1676 93 31 BsiEI CG_ry'CG Cuts at: 0 1071 1800 Size: 1071 729 BsiHKAI G_wGCw'C Cuts at: 0 973 1800 Size: 973 827 BsmI GAATG_Cn' Cuts at: 0 893 1312 1800 Size: 893 419 488 Fragments arranged by size: 893 488 419 Bsp1286I G_dGCh'C Cuts at: 0 22 705 973 1427 1800 Size: 22 683 268 454 373 Fragments arranged by size: 683 454 373 268 22 BspEI T'CCGG_A Cuts at: 0 446 650 1800 Size: 446 204 1150 Fragments arranged by size: 1150 446 204 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 45 1800 Size: 45 1755 BsrBI CCG'CTC Cuts at: 0 643 1800 Size: 643 1157 BsrDI GCAATG_nn' Cuts at: 0 1047 1800 Size: 1047 753 BsrFI r'CCGG_y Cuts at: 0 99 1800 Size: 99 1701 BssHII G'CGCG_C Cuts at: 0 65 1800 Size: 65 1735 BstXI CCAn_nnnn'nTGG Cuts at: 0 1233 1800 Size: 1233 567 BstYI r'GATC_y Cuts at: 0 468 926 1332 1775 1800 Size: 468 458 406 443 25 Fragments arranged by size: 468 458 443 406 25 BtgI C'CryG_G Cuts at: 0 1089 1121 1226 1427 1800 Size: 1089 32 105 201 373 Fragments arranged by size: 1089 373 201 105 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1045 1800 Size: 1045 755 BtsI GCAGTG_nn' Cuts at: 0 705 1800 Size: 705 1095 DraI TTT'AAA Cuts at: 0 1458 1800 Size: 1458 342 EaeI y'GGCC_r Cuts at: 0 281 416 473 1086 1800 Size: 281 135 57 613 714 Fragments arranged by size: 714 613 281 135 57 EarI CTCTTCn'nnn_ Cuts at: 0 213 218 1121 1503 1616 1800 Size: 213 5 903 382 113 184 Fragments arranged by size: 903 382 213 184 113 5 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 389 407 586 804 1025 1516 1730 1800 Size: 389 18 179 218 221 491 214 70 Fragments arranged by size: 491 389 221 218 214 179 70 18 EcoO109I rG'GnC_Cy Cuts at: 0 300 1800 Size: 300 1500 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1678 1800 Size: 1678 122 HaeII r_GCGC'y Cuts at: 0 1103 1800 Size: 1103 697 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 242 274 289 321 1800 Size: 242 32 15 32 1479 Fragments arranged by size: 1479 242 32 32 15 HincII GTy'rAC Cuts at: 0 1011 1736 1800 Size: 1011 725 64 Fragments arranged by size: 1011 725 64 HindIII A'AGCT_T Cuts at: 0 1268 1289 1556 1800 Size: 1268 21 267 244 Fragments arranged by size: 1268 267 244 21 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 228 488 1800 Size: 228 260 1312 Fragments arranged by size: 1312 260 228 MscI TGG'CCA Cuts at: 0 283 418 475 1088 1800 Size: 283 135 57 613 712 Fragments arranged by size: 712 613 283 135 57 MslI CAynn'nnrTG Cuts at: 0 412 976 1800 Size: 412 564 824 Fragments arranged by size: 824 564 412 MspA1I CmG'CkG Cuts at: 0 45 479 771 1201 1800 Size: 45 434 292 430 599 Fragments arranged by size: 599 434 430 292 45 NcoI C'CATG_G Cuts at: 0 1121 1226 1800 Size: 1121 105 574 Fragments arranged by size: 1121 574 105 NdeI CA'TA_TG Cuts at: 0 1281 1800 Size: 1281 519 NspI r_CATG'y Cuts at: 0 10 444 1800 Size: 10 434 1356 Fragments arranged by size: 1356 434 10 PciI A'CATG_T Cuts at: 0 440 1800 Size: 440 1360 PfoI T'CCnGG_A Cuts at: 0 200 366 1493 1800 Size: 200 166 1127 307 Fragments arranged by size: 1127 307 200 166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 427 459 1800 Size: 427 32 1341 Fragments arranged by size: 1341 427 32 PpuMI rG'GwC_Cy Cuts at: 0 300 1800 Size: 300 1500 PspXI vC'TCGA_Gb Cuts at: 0 1355 1800 Size: 1355 445 PstI C_TGCA'G Cuts at: 0 153 1175 1800 Size: 153 1022 625 Fragments arranged by size: 1022 625 153 PvuII CAG'CTG Cuts at: 0 479 771 1800 Size: 479 292 1029 Fragments arranged by size: 1029 479 292 SbfI CC_TGCA'GG Cuts at: 0 1175 1800 Size: 1175 625 SexAI A'CCwGG_T Cuts at: 0 632 809 1800 Size: 632 177 991 Fragments arranged by size: 991 632 177 SfcI C'TryA_G Cuts at: 0 149 1171 1800 Size: 149 1022 629 Fragments arranged by size: 1022 629 149 SgrAI Cr'CCGG_yG Cuts at: 0 99 1800 Size: 99 1701 SmaI CCC'GGG Cuts at: 0 988 1800 Size: 988 812 SmlI C'TyrA_G Cuts at: 0 272 1219 1355 1690 1800 Size: 272 947 136 335 110 Fragments arranged by size: 947 335 272 136 110 StuI AGG'CCT Cuts at: 0 596 1800 Size: 596 1204 StyI C'CwwG_G Cuts at: 0 1121 1226 1800 Size: 1121 105 574 Fragments arranged by size: 1121 574 105 Tth111I GACn'n_nGTC Cuts at: 0 298 1800 Size: 298 1502 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 276 1800 Size: 276 1524 XhoI C'TCGA_G Cuts at: 0 272 1355 1800 Size: 272 1083 445 Fragments arranged by size: 1083 445 272 XmaI C'CCGG_G Cuts at: 0 986 1800 Size: 986 814 XmnI GAAnn'nnTTC Cuts at: 0 209 322 1658 1800 Size: 209 113 1336 142 Fragments arranged by size: 1336 209 142 113 Enzymes that do cut: AarI AcuI AfeI AflII AflIII AleI AloI AlwNI ApaLI ApoI AvaI BanII BbsI BbvCI BcgI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstXI BstYI BtgI BtgZI BtsI DraI EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NdeI NspI PciI PfoI PpiI PpuMI PspXI PstI PvuII SbfI SexAI SfcI SgrAI SmaI SmlI StuI StyI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AgeI AhdI AlfI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BciVI BclI BfrBI BlpI BmgBI BmtI BsaAI BsaBI BsiWI BsmBI BspHI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI XbaI ZraI