################################## # NO CUT ENZYMES ( 216 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MroI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 125 GCGG CCGC(-3/-1) AfaI 49 GTAC GT^AC Aor51HI 5 AGCGCT AGC^GCT Asp700I 3 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 149 TGGCCA TGG^CCA BbvCI 104 CCTCAGC CCTCAGC(-5/-2) BbvI 98 GCAGC GCAGC(8/12) BsiYI 39 CCTGGTTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 39 CCTGGTTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrI 61 ACTGG ACTGG(1/-1) BssKI 39 CCTGG ^CC[ACGT]GG BstF5I 110 CATCC GGATG(2/0) BstNI 39 CCTGG CC^[AT]GG CfoI 6 GCGC GCG^C EaeI 149 TGGCCA [CT]^GGCC[GA] Eco47III 5 AGCGCT AGC^GCT EcoRII 39 CCTGG ^CC[AT]GG Fnu4HI 98 GCAGC GC^[ACGT]GC FokI 110 CATCC GGATG(9/13) HaeII 5 AGCGCT [GA]GCGC^[CT] HaeIII 150 GGCC GG^CC HhaI 6 GCGC GCG^C HinP1I 6 GCGC G^CGC HinfI 65 GACTC G^A[ACGT]TC HphI 26 TCACC GGTGA(8/7) ItaI 98 GCAGC GC^[ACGT]GC MluNI 149 TGGCCA TGG^CCA MscI 149 TGGCCA TGG^CCA MvaI 39 CCTGG CC^[AT]GG PleI 65 GACTC GAGTC(4/5) RsaI 49 GTAC GT^AC ScrFI 39 CCTGG CC^[ACGT]GG SfaNI 109 GCATC GCATC(5/9) TseI 98 GCAGC G^C[AT]GC XcmI 29 CCATCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 3 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 4 enzymes) # ################################### DdeI 69 CTCAG C^T[ACGT]AG 105 CTCAG EarI 13 CTCTTC CTCTTC(1/4) 169 CTCTTC Ksp632I 13 CTCTTC CTCTTC(1/4) 169 CTCTTC MwoI 89 GCCAAACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 116 GCAACATAAGC ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MboII MnlI