(Circular) (Six-base) MAPSORT of: pFN21AE5085.seq Check: 3508 from: 1 to: 4888 pFN21AE5085 4888 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2698 278 Size: 53 83 186 936 1162 2468 Fragments arranged by size: 2468 1162 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2231 1937 Size: 294 4594 Acc65I G'GTAC_C Cuts at: 2209 2209 Size: 4888 AclI AA'CG_TT Cuts at: 1563 3015 3388 1563 Size: 1452 373 3063 Fragments arranged by size: 3063 1452 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2167 2951 4335 4603 1632 Size: 535 784 1384 268 1917 Fragments arranged by size: 1917 1384 784 535 268 AfeI AGC'GCT Cuts at: 2012 2012 Size: 4888 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4691 AflIII A'CryG_T Cuts at: 1203 3787 4764 1203 Size: 2584 977 1327 Fragments arranged by size: 2584 1327 977 AgeI A'CCGG_T Cuts at: 3719 3719 Size: 4888 AhdI GACnn_n'nnGTC Cuts at: 3617 3617 Size: 4888 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4888 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4888 AlwNI CAG_nnn'CTG Cuts at: 2631 4203 2631 Size: 1572 3316 ApaLI G'TGCA_C Cuts at: 2945 4101 2945 Size: 1156 3732 ApoI r'AATT_y Cuts at: 1493 2197 2423 2575 4684 1493 Size: 704 226 152 2109 1697 Fragments arranged by size: 2109 1697 704 226 152 AseI AT'TA_AT Cuts at: 160 1032 3442 160 Size: 872 2410 1606 Fragments arranged by size: 2410 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4888 AvaI C'yCGr_G Cuts at: 1870 1943 2213 1870 Size: 73 270 4545 Fragments arranged by size: 4545 270 73 BamHI G'GATC_C Cuts at: 2218 2585 2218 Size: 367 4521 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2209 3664 618 Size: 325 217 627 422 1455 1842 Fragments arranged by size: 1842 1455 627 422 325 217 BanII G_rGCy'C Cuts at: 1391 2207 1391 Size: 816 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3917 BbvCI CC'TCA_GC Cuts at: 2110 2110 Size: 4888 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3079 3113 1903 Size: 34 1142 34 3678 Fragments arranged by size: 3678 1142 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2780 3990 2780 Size: 1210 3678 BclI T'GATC_A Cuts at: 1548 1548 Size: 4888 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3499 136 Size: 107 122 71 631 605 1827 1525 Fragments arranged by size: 1827 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4883 4883 Size: 4888 BlpI GC'TnA_GC Cuts at: 2305 2305 Size: 4888 Bme1580I G_kGCm'C Cuts at: 1790 2949 4105 1790 Size: 1159 1156 2573 Fragments arranged by size: 2573 1159 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4888 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2619 3577 442 Size: 416 53 83 399 24 1202 958 1753 Fragments arranged by size: 1753 1202 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4888 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3548 1239 Size: 2309 2579 Bpu10I CC'TnA_GC Cuts at: 2110 2110 Size: 4888 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3013 3878 4176 3013 Size: 865 298 3725 Fragments arranged by size: 3725 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3551 882 Size: 617 2052 2219 Fragments arranged by size: 2219 2052 617 BsaAI yAC'GTr Cuts at: 493 1204 2679 493 Size: 711 1475 2702 Fragments arranged by size: 2702 1475 711 BsaBI GATnn'nnATC Cuts at: 4882 4882 Size: 4888 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2695 3077 3712 275 Size: 53 83 186 936 1162 382 635 1451 Fragments arranged by size: 1451 1162 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3321 3719 3993 4140 4551 3321 Size: 398 274 147 411 3658 Fragments arranged by size: 3658 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4663 Fragments arranged by size: 4663 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4888 BseYI C'CCAG_C Cuts at: 4091 4587 4091 Size: 496 4392 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2338 3099 4127 1540 Size: 275 186 337 761 1028 2301 Fragments arranged by size: 2301 1028 761 337 275 186 BsiHKAI G_wGCw'C Cuts at: 2949 3034 4105 2949 Size: 85 1071 3732 Fragments arranged by size: 3732 1071 85 BsmI GAATG_Cn' Cuts at: 2399 2492 2399 Size: 93 4795 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2207 2949 3034 4105 1391 Size: 399 417 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 417 399 85 BspEI T'CCGG_A Cuts at: 4551 4551 Size: 4888 BspHI T'CATG_A Cuts at: 2777 4507 2777 Size: 1730 3158 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2243 844 Size: 1399 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2775 1394 Size: 429 952 3507 Fragments arranged by size: 3507 952 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3383 3557 65 Size: 1183 2135 174 1396 Fragments arranged by size: 2135 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3532 3719 1700 Size: 110 1722 187 2869 Fragments arranged by size: 2869 1722 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4888 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4888 BssSI C'ACGA_G Cuts at: 2948 3960 2948 Size: 1012 3876 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4888 BstBI TT'CG_AA Cuts at: 3692 3692 Size: 4888 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4888 BstYI r'GATC_y Cuts at: 1972 2218 2585 2970 2987 4439 4525 4537 4883 Size: 246 367 385 17 1452 86 12 346 Cuts at: 4883 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 246 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4336 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4149 Fragments arranged by size: 4149 683 56 BtsI GCAGTG_nn' Cuts at: 2404 3198 3218 2404 Size: 794 20 4074 Fragments arranged by size: 4074 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4888 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4853 DraI TTT'AAA Cuts at: 2192 2192 Size: 4888 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3895 809 Size: 507 2579 1802 Fragments arranged by size: 2579 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 2335 3224 Size: 54 1425 50 275 18 324 181 889 Cuts at: 3224 4853 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 324 275 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2335 1537 Size: 275 523 4090 Fragments arranged by size: 4090 523 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2180 2818 1353 Size: 671 156 638 3423 Fragments arranged by size: 3423 671 638 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3475 3849 3995 387 Size: 1064 138 1886 374 146 1280 Fragments arranged by size: 1886 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2167 2951 3548 4335 4603 1239 Size: 393 535 784 597 787 268 1524 Fragments arranged by size: 1524 787 784 597 535 393 268 EcoRI G'AATT_C Cuts at: 2197 2575 2197 Size: 378 4510 FspI TGC'GCA Cuts at: 3394 3394 Size: 4888 HaeII r_GCGC'y Cuts at: 1678 2014 4035 1678 Size: 336 2021 2531 Fragments arranged by size: 2531 2021 336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3529 3561 3603 3635 1373 Size: 32 135 32 1957 32 42 32 2626 Fragments arranged by size: 2626 1957 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2232 2478 669 Size: 988 281 294 246 3079 Fragments arranged by size: 3079 988 294 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4888 HpaI GTT'AAC Cuts at: 2478 2478 Size: 4888 KpnI G_GTAC'C Cuts at: 2213 2213 Size: 4888 MfeI C'AATT_G Cuts at: 2487 2487 Size: 4888 MluI A'CGCG_T Cuts at: 4764 4764 Size: 4888 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4002 4186 1305 Size: 2697 184 2007 Fragments arranged by size: 2697 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2156 10 Size: 54 1425 343 324 2742 Fragments arranged by size: 2742 1425 343 324 54 MslI CAynn'nnrTG Cuts at: 518 1166 2846 3205 3364 518 Size: 648 1680 359 159 2042 Fragments arranged by size: 2042 1680 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2304 2981 4129 4374 1606 Size: 698 677 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4336 NdeI CA'TA_TG Cuts at: 387 387 Size: 4888 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4888 NotI GC'GGCC_GC Cuts at: 2335 2335 Size: 4888 NspI r_CATG'y Cuts at: 1132 1229 2246 3791 1132 Size: 97 1017 1545 2229 Fragments arranged by size: 2229 1545 1017 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4888 PciI A'CATG_T Cuts at: 3787 3787 Size: 4888 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4888 PmeI GTTT'AAAC Cuts at: 2192 2192 Size: 4888 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2957 2989 4470 4502 2957 Size: 32 1481 32 3343 Fragments arranged by size: 3343 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4888 PsiI TTA'TAA Cuts at: 2458 2458 Size: 4888 PstI C_TGCA'G Cuts at: 830 2240 830 Size: 1410 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4888 SalI G'TCGA_C Cuts at: 1936 2230 1936 Size: 294 4594 SbfI CC_TGCA'GG Cuts at: 2240 2240 Size: 4888 ScaI AGT'ACT Cuts at: 3136 4848 3136 Size: 1712 3176 SfcI C'TryA_G Cuts at: 826 1045 2236 3371 4052 4243 826 Size: 219 1191 1135 681 191 1471 Fragments arranged by size: 1471 1191 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4888 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4888 SmaI CCC'GGG Cuts at: 1872 2215 1872 Size: 343 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2992 3893 4155 820 Size: 197 926 1049 901 262 1553 Fragments arranged by size: 1553 1049 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2679 493 Size: 2186 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4888 SphI G_CATG'C Cuts at: 1132 2246 1132 Size: 1114 3774 SspI AAT'ATT Cuts at: 5 52 2812 5 Size: 47 2760 2081 Fragments arranged by size: 2760 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4888 StyI C'CwwG_G Cuts at: 513 1065 2327 513 Size: 552 1262 3074 Fragments arranged by size: 3074 1262 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2915 2932 3085 3270 3715 325 Size: 1255 1335 17 153 185 445 1498 Fragments arranged by size: 1498 1335 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3134 4846 96 Size: 274 80 33 51 1443 1157 1712 138 Fragments arranged by size: 1712 1443 1157 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4848 4880 4848 Size: 32 4856 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4888 XbaI T'CTAG_A Cuts at: 2224 2224 Size: 4888 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2041 2041 Size: 4888 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4888 XmaI C'CCGG_G Cuts at: 1870 2213 1870 Size: 343 4545 XmnI GAAnn'nnTTC Cuts at: 2012 3017 2012 Size: 1005 3883 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2696 276 Size: 53 83 186 936 1162 2468 Fragments arranged by size: 2468 1162 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI