################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AscI AseI AsnI Asp700I Asp718I AspI AviII BamHI BbrPI BcgI BfrI BlpI Bpu1102I BsaAI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspEI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I CelII CpoI DrdI EagI EclXI Eco52I EcoNI EcoRI FseI FspI HincII HindII HindIII HpaI KpnI MfeI MluI MroI MslI MunI NcoI NheI NotI NruI NspV PacI PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I SspBI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 1949 GACGTC GACGT^C AccI 1570 GTAGAC GT^[AC][GT]AC AfaI 1241 GTAC GT^AC AgeI 340 ACCGGT A^CCGGT AhdI 2602 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2255 GTGCAC G^TGCAC AlwI 2043 GGATC GGATC(4/5) AocI 1933 CCTGAGG CC^T[ACGT]AGG Aor51HI 146 AGCGCT AGC^GCT ApaI 2557 GGGCCC GGGCC^C ApaLI 2255 GTGCAC G^TGCAC AspEI 2602 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1915 CCTAGG C^CTAGG BalI 2819 TGGCCA TGG^CCA BbeI 761 GGCGCC GGCGC^C BciVI 901 GGATAC GTATCC(6/5) BglI 2323 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1890 AGATCT A^GATCT BlnI 1915 CCTAGG C^CTAGG BspMI 1057 GCAGGT ACCTGC(4/8) BssSI 1415 CACGAG CACGAG(-5/-1) BstYI 1890 AGATCT [GA]^GATC[CT] Bsu36I 1933 CCTGAGG CC^T[ACGT]AGG DraI 2682 TTTAAA TTT^AAA Eam1105I 2602 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 146 AGCGCT AGC^GCT Eco81I 1933 CCTGAGG CC^T[ACGT]AGG EcoRV 1210 GATATC GAT^ATC EcoT22I 2407 ATGCAT ATGCA^T KasI 761 GGCGCC G^GCGCC MflI 1890 AGATCT [GA]^GATC[CT] MluNI 2819 TGGCCA TGG^CCA MscI 2819 TGGCCA TGG^CCA NaeI 758 GCCGGC GCC^GGC NarI 761 GGCGCC GG^CGCC NdeI 1525 CATATG CA^TATG NgoMIV 758 GCCGGC G^CCGGC NsiI 2407 ATGCAT ATGCA^T PaeR7I 971 CTCGAG C^TCGAG PflMI 1540 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 340 ACCGGT A^CCGGT PshAI 2014 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 1241 GTAC GT^AC ScaI 1240 AGTACT AGT^ACT SexAI 2840 ACCTGGT A^CC[AT]GGT SfoI 761 GGCGCC GGC^GCC SmaI 431 CCCGGG CCC^GGG SpeI 1237 ACTAGT A^CTAGT Van91I 1540 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 971 CTCGAG C^TCGAG XhoII 1890 AGATCT [GA]^GATC[CT] XmaCI 431 CCCGGG C^CCGGG XmaI 431 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BanII 225 GGGCTC G[GA]GC[CT]^C 2557 GGGCCC BbvCI 473 CCTCAGC CCTCAGC(-5/-2) 2621 CCTCAGC BsaBI 790 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1885 GATCGAGATC BsmBI 2500 CGTCTC CGTCTC(1/5) 107 GAGACG BspDI 1427 ATCGAT AT^CGAT 2066 ATCGAT BspHI 573 TCATGA T^CATGA 2846 TCATGA BstEII 835 GGTCACC G^GT[ACGT]ACC 2422 GGTGACC BstPI 835 GGTCACC G^GT[ACGT]ACC 2422 GGTGACC BstXI 2573 CCATTCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2810 CCAAGGCACTGG ClaI 1427 ATCGAT AT^CGAT 2066 ATCGAT DraIII 1541 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG 2771 CACCTTGTG EcoO65I 835 GGTCACC G^GT[ACGT]ACC 2422 GGTGACC MamI 790 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1885 GATCGAGATC MseI 1168 TTAA T^TAA 2683 TTAA PvuII 2171 CAGCTG CAG^CTG 2624 CAGCTG RcaI 573 TCATGA T^CATGA 2846 TCATGA SapI 2130 GAAGAGC GCTCTTC(1/4) 1125 GCTCTTC StuI 1219 AGGCCT AGG^CCT 1380 AGGCCT Tru9I 1168 TTAA T^TAA 2683 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AcyI AluI AlwNI ApoI AspHI AvaI AvaII BanI BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI