(Linear) (Six-base) MAPSORT of: KIEE5083.seq Check: 6794 from: 1 to: 2931 KIEE5083.orf With 182 enzymes: SgfI * December 5, 2014 12:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1048 2931 Size: 1048 1883 AatII G_ACGT'C Cuts at: 0 1953 2931 Size: 1953 978 AccI GT'mk_AC Cuts at: 0 1571 2931 Size: 1571 1360 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 754 2308 2401 2849 2931 Size: 535 219 1554 93 448 82 Fragments arranged by size: 1554 535 448 219 93 82 AfeI AGC'GCT Cuts at: 0 148 2931 Size: 148 2783 AgeI A'CCGG_T Cuts at: 0 340 2931 Size: 340 2591 AhdI GACnn_n'nnGTC Cuts at: 0 2607 2931 Size: 2607 324 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1379 1411 2659 2691 2931 Size: 1379 32 1248 32 240 Fragments arranged by size: 1379 1248 240 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1063 1611 1620 2326 2638 2796 2931 Size: 1063 548 9 706 312 158 135 Fragments arranged by size: 1063 706 548 312 158 135 9 ApaI G_GGCC'C Cuts at: 0 2561 2931 Size: 2561 370 ApaLI G'TGCA_C Cuts at: 0 2255 2931 Size: 2255 676 ApoI r'AATT_y Cuts at: 0 396 1258 1420 2931 Size: 396 862 162 1511 Fragments arranged by size: 1511 862 396 162 AvaI C'yCGr_G Cuts at: 0 384 431 971 1096 1691 2931 Size: 384 47 540 125 595 1240 Fragments arranged by size: 1240 595 540 384 125 47 AvrII C'CTAG_G Cuts at: 0 1915 2931 Size: 1915 1016 BanI G'GyrC_C Cuts at: 0 761 842 1435 1668 1758 2931 Size: 761 81 593 233 90 1173 Fragments arranged by size: 1173 761 593 233 90 81 BanII G_rGCy'C Cuts at: 0 229 2561 2931 Size: 229 2332 370 Fragments arranged by size: 2332 370 229 BbeI G_GCGC'C Cuts at: 0 765 2931 Size: 765 2166 BbsI GAAGACnn'nnnn_ Cuts at: 0 2071 2243 2437 2931 Size: 2071 172 194 494 Fragments arranged by size: 2071 494 194 172 BbvCI CC'TCA_GC Cuts at: 0 474 2622 2931 Size: 474 2148 309 Fragments arranged by size: 2148 474 309 BciVI GTATCCnnnnn_n' Cuts at: 0 895 2931 Size: 895 2036 BclI T'GATC_A Cuts at: 0 1104 2708 2849 2931 Size: 1104 1604 141 82 Fragments arranged by size: 1604 1104 141 82 BfrBI ATG'CAT Cuts at: 0 2409 2931 Size: 2409 522 BglI GCCn_nnn'nGGC Cuts at: 0 2329 2931 Size: 2329 602 BglII A'GATC_T Cuts at: 0 1890 2931 Size: 1890 1041 Bme1580I G_kGCm'C Cuts at: 0 1671 2259 2561 2931 Size: 1671 588 302 370 Fragments arranged by size: 1671 588 370 302 BmrI ACTGGGnnnn_n' Cuts at: 0 1768 2931 Size: 1768 1163 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 130 1241 1706 2931 Size: 130 1111 465 1225 Fragments arranged by size: 1225 1111 465 130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 339 872 1000 1073 2486 2931 Size: 339 533 128 73 1413 445 Fragments arranged by size: 1413 533 445 339 128 73 Bpu10I CC'TnA_GC Cuts at: 0 474 2622 2931 Size: 474 2148 309 Fragments arranged by size: 2148 474 309 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1054 2470 2931 Size: 1054 1416 461 Fragments arranged by size: 1416 1054 461 BsaI GGTCTCn'nnnn_ Cuts at: 0 968 1901 1970 2144 2931 Size: 968 933 69 174 787 Fragments arranged by size: 968 933 787 174 69 BsaBI GATnn'nnATC Cuts at: 0 794 1889 2931 Size: 794 1095 1042 Fragments arranged by size: 1095 1042 794 BsaHI Gr'CG_yC Cuts at: 0 762 1560 1950 2931 Size: 762 798 390 981 Fragments arranged by size: 981 798 762 390 BsaWI w'CCGG_w Cuts at: 0 340 1118 2192 2931 Size: 340 778 1074 739 Fragments arranged by size: 1074 778 739 340 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 783 813 2187 2217 2459 2489 2931 Size: 783 30 1374 30 242 30 442 Fragments arranged by size: 1374 783 442 242 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 462 1951 2604 2931 Size: 462 1489 653 327 Fragments arranged by size: 1489 653 462 327 BseYI C'CCAG_C Cuts at: 0 536 1897 2480 2786 2931 Size: 536 1361 583 306 145 Fragments arranged by size: 1361 583 536 306 145 BsiHKAI G_wGCw'C Cuts at: 0 391 719 1692 2259 2931 Size: 391 328 973 567 672 Fragments arranged by size: 973 672 567 391 328 BsmBI CGTCTCn'nnnn_ Cuts at: 0 101 2506 2931 Size: 101 2405 425 Fragments arranged by size: 2405 425 101 Bsp1286I G_dGCh'C Cuts at: 0 229 391 719 1671 1692 2259 2561 2931 Size: 229 162 328 952 21 567 302 370 Fragments arranged by size: 952 567 370 328 302 229 162 21 BspHI T'CATG_A Cuts at: 0 573 2846 2931 Size: 573 2273 85 Fragments arranged by size: 2273 573 85 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1048 2931 Size: 1048 1883 BsrBI CCG'CTC Cuts at: 0 207 645 773 2243 2931 Size: 207 438 128 1470 688 Fragments arranged by size: 1470 688 438 207 128 BsrDI GCAATG_nn' Cuts at: 0 246 964 1304 2931 Size: 246 718 340 1627 Fragments arranged by size: 1627 718 340 246 BsrFI r'CCGG_y Cuts at: 0 340 725 758 839 914 2606 2931 Size: 340 385 33 81 75 1692 325 Fragments arranged by size: 1692 385 340 325 81 75 33 BssSI C'ACGA_G Cuts at: 0 1415 2931 Size: 1415 1516 BstAPI GCAn_nnn'nTGC Cuts at: 0 1063 1510 1611 1620 2370 2415 2931 Size: 1063 447 101 9 750 45 516 Fragments arranged by size: 1063 750 516 447 101 45 9 BstEII G'GTnAC_C Cuts at: 0 835 2422 2931 Size: 835 1587 509 Fragments arranged by size: 1587 835 509 BstXI CCAn_nnnn'nTGG Cuts at: 0 2580 2817 2931 Size: 2580 237 114 Fragments arranged by size: 2580 237 114 BstYI r'GATC_y Cuts at: 0 1890 2931 Size: 1890 1041 Bsu36I CC'TnA_GG Cuts at: 0 1934 2931 Size: 1934 997 BtgI C'CryG_G Cuts at: 0 28 102 174 198 2859 2931 Size: 28 74 72 24 2661 72 Fragments arranged by size: 2661 74 72 72 28 24 BtsI GCAGTG_nn' Cuts at: 0 1995 2270 2636 2931 Size: 1995 275 366 295 Fragments arranged by size: 1995 366 295 275 ClaI AT'CG_AT Cuts at: 0 1428 2067 2931 Size: 1428 639 864 Fragments arranged by size: 1428 864 639 DraI TTT'AAA Cuts at: 0 2684 2931 Size: 2684 247 DraIII CAC_nnn'GTG Cuts at: 0 1546 2776 2931 Size: 1546 1230 155 Fragments arranged by size: 1546 1230 155 EaeI y'GGCC_r Cuts at: 0 670 689 936 1230 2819 2931 Size: 670 19 247 294 1589 112 Fragments arranged by size: 1589 670 294 247 112 19 EarI CTCTTCn'nnn_ Cuts at: 0 1132 2125 2622 2896 2931 Size: 1132 993 497 274 35 Fragments arranged by size: 1132 993 497 274 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 48 300 947 2931 Size: 48 252 647 1984 Fragments arranged by size: 1984 647 252 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 339 535 754 872 1000 1073 2308 2401 Size: 339 196 219 118 128 73 1235 93 Cuts at: 2401 2486 2849 2931 Size: 85 363 82 Fragments arranged by size: 1235 363 339 219 196 128 118 93 85 82 73 EcoO109I rG'GnC_Cy Cuts at: 0 18 533 821 1387 1734 2093 2557 2754 Size: 18 515 288 566 347 359 464 197 Cuts at: 2754 2931 Size: 177 Fragments arranged by size: 566 515 464 359 347 288 197 177 18 EcoRV GAT'ATC Cuts at: 0 1212 2931 Size: 1212 1719 HaeII r_GCGC'y Cuts at: 0 150 765 893 2920 2931 Size: 150 615 128 2027 11 Fragments arranged by size: 2027 615 150 128 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 517 549 1846 1878 2187 2219 2593 2625 Size: 517 32 1297 32 309 32 374 32 Cuts at: 2625 2931 Size: 306 Fragments arranged by size: 1297 517 374 309 306 32 32 32 32 KasI G'GCGC_C Cuts at: 0 761 2931 Size: 761 2170 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 141 2426 2504 2931 Size: 141 2285 78 427 Fragments arranged by size: 2285 427 141 78 MscI TGG'CCA Cuts at: 0 2821 2931 Size: 2821 110 MspA1I CmG'CkG Cuts at: 0 30 63 104 200 254 635 2173 2626 Size: 30 33 41 96 54 381 1538 453 Cuts at: 2626 2931 Size: 305 Fragments arranged by size: 1538 453 381 305 96 54 41 33 30 NaeI GCC'GGC Cuts at: 0 760 2931 Size: 760 2171 NarI GG'CG_CC Cuts at: 0 762 2931 Size: 762 2169 NdeI CA'TA_TG Cuts at: 0 1526 2931 Size: 1526 1405 NgoMIV G'CCGG_C Cuts at: 0 758 2931 Size: 758 2173 NsiI A_TGCA'T Cuts at: 0 2411 2931 Size: 2411 520 NspI r_CATG'y Cuts at: 0 700 1513 2373 2409 2931 Size: 700 813 860 36 522 Fragments arranged by size: 860 813 700 522 36 PflMI CCAn_nnn'nTGG Cuts at: 0 1546 2931 Size: 1546 1385 PfoI T'CCnGG_A Cuts at: 0 2795 2931 Size: 2795 136 PpuMI rG'GwC_Cy Cuts at: 0 18 533 1387 1734 2754 2931 Size: 18 515 854 347 1020 177 Fragments arranged by size: 1020 854 515 347 177 18 PshAI GACnn'nnGTC Cuts at: 0 2018 2931 Size: 2018 913 PsiI TTA'TAA Cuts at: 0 1315 2931 Size: 1315 1616 PspOMI G'GGCC_C Cuts at: 0 2557 2931 Size: 2557 374 PstI C_TGCA'G Cuts at: 0 923 1616 1803 2931 Size: 923 693 187 1128 Fragments arranged by size: 1128 923 693 187 PvuII CAG'CTG Cuts at: 0 2173 2626 2931 Size: 2173 453 305 Fragments arranged by size: 2173 453 305 SacII CC_GC'GG Cuts at: 0 31 105 201 2931 Size: 31 74 96 2730 Fragments arranged by size: 2730 96 74 31 SanDI GG'GwC_CC Cuts at: 0 18 533 2931 Size: 18 515 2398 Fragments arranged by size: 2398 515 18 SapI GCTCTTCn'nnn_ Cuts at: 0 1132 2125 2931 Size: 1132 993 806 Fragments arranged by size: 1132 993 806 ScaI AGT'ACT Cuts at: 0 1242 2931 Size: 1242 1689 SexAI A'CCwGG_T Cuts at: 0 2840 2931 Size: 2840 91 SfcI C'TryA_G Cuts at: 0 827 919 1612 1799 2931 Size: 827 92 693 187 1132 Fragments arranged by size: 1132 827 693 187 92 SfoI GGC'GCC Cuts at: 0 763 2931 Size: 763 2168 SmaI CCC'GGG Cuts at: 0 433 2931 Size: 433 2498 SmlI C'TyrA_G Cuts at: 0 971 1033 2485 2931 Size: 971 62 1452 446 Fragments arranged by size: 1452 971 446 62 SpeI A'CTAG_T Cuts at: 0 1237 2931 Size: 1237 1694 SphI G_CATG'C Cuts at: 0 700 1513 2373 2931 Size: 700 813 860 558 Fragments arranged by size: 860 813 700 558 StuI AGG'CCT Cuts at: 0 1221 1382 2931 Size: 1221 161 1549 Fragments arranged by size: 1549 1221 161 StyI C'CwwG_G Cuts at: 0 1039 1390 1865 1915 2810 2931 Size: 1039 351 475 50 895 121 Fragments arranged by size: 1039 895 475 351 121 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 277 1362 1371 1746 2931 Size: 277 1085 9 375 1185 Fragments arranged by size: 1185 1085 375 277 9 TatI w'GTAC_w Cuts at: 0 1240 2931 Size: 1240 1691 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1760 1792 2459 2491 2931 Size: 1760 32 667 32 440 Fragments arranged by size: 1760 667 440 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 708 2308 2352 2931 Size: 708 1600 44 579 Fragments arranged by size: 1600 708 579 44 XhoI C'TCGA_G Cuts at: 0 971 2931 Size: 971 1960 XmaI C'CCGG_G Cuts at: 0 431 2931 Size: 431 2500 ZraI GAC'GTC Cuts at: 0 1951 2931 Size: 1951 980 Enzymes that do cut: AarI AatII AccI AcuI AfeI AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRV HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PflMI PfoI PpuMI PshAI PsiI PspOMI PstI PvuII SacII SanDI SapI ScaI SexAI SfcI SfoI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmaI ZraI Enzymes that do not cut: Acc65I AclI AflII AflIII AleI AlfI AscI AseI AsiSI BaeI BamHI BcgI BlpI BmgBI BmtI BsaAI BsgI BsiEI BsiWI BsmI BspEI BsrGI BssHII BstBI BstZ17I BtgZI CspCI DrdI EagI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NcoI NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacI SalI SbfI SfiI SgfI SgrAI SnaBI SrfI SspI SwaI Tth111I XbaI XmnI