################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccIII AclI AgeI AhdI AocI Aor51HI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvI BcgI BciVI BcnI BfaI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI Fnu4HI FseI FspI HaeII HhaI HinP1I HindIII HpaI HphI ItaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 418 GACGTC GACGT^C AccII 503 CGCG CG^CG AcyI 418 GACGTC G[GA]^CG[CT]C AflII 39 CTTAAG C^TTAAG AflIII 891 ACATGT A^C[GA][CT]GT Alw44I 1183 GTGCAC G^TGCAC AlwNI 1223 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 663 GGGCCC GGGCC^C ApaLI 1183 GTGCAC G^TGCAC AseI 604 ATTAAT AT^TAAT AsnI 604 ATTAAT AT^TAAT BamHI 480 GGATCC G^GATCC BanII 663 GGGCCC G[GA]GC[CT]^C BfrI 39 CTTAAG C^TTAAG BpmI 935 CTCCAG CTGGAG(16/14) BsaHI 418 GACGTC G[GA]^CG[CT]C BsaI 404 GGTCTC GGTCTC(1/5) BsgI 1031 GTGCAG GTGCAG(16/14) BsmFI 416 GGGAC GGGAC(10/14) BspLU11I 891 ACATGT A^CATGT BstAPI 1118 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 854 TTCGAA TT^CGAA BstUI 503 CGCG CG^CG EaeI 778 TGGCCG [CT]^GGCC[GA] EarI 272 GAAGAG CTCTTC(1/4) HapII 781 CCGG C^CGG HgaI 504 GCGTC GACGC(5/10) Hin1I 418 GACGTC G[GA]^CG[CT]C HincII 1079 GTCAAC GT[CT]^[GA]AC HindII 1079 GTCAAC GT[CT]^[GA]AC HpaII 781 CCGG C^CGG Ksp632I 272 GAAGAG CTCTTC(1/4) MaeIII 580 GTAAC ^GT[ACGT]AC MslI 1024 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 781 CCGG C^CGG MvnI 503 CGCG CG^CG NspI 891 ACATGT [GA]CATG^[CT] NspV 854 TTCGAA TT^CGAA PflMI 123 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 933 GACTC GAGTC(4/5) PshBI 604 ATTAAT AT^TAAT SfaNI 1108 GATGC GCATC(5/9) SfuI 854 TTCGAA TT^CGAA TaqI 855 TCGA T^CGA TthHB8I 855 TCGA T^CGA Van91I 123 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AciI 215 CCGC CCGC(-3/-1) 502 CCGC AfaI 157 GTAC GT^AC 909 GTAC Asp700I 22 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 642 GAAAGCCTTC BbsI 712 GAAGAC GAAGAC(2/6) 982 GAAGAC BbvCI 549 CCTCAGC CCTCAGC(-5/-2) 411 GCTGAGG BpuAI 712 GAAGAC GAAGAC(2/6) 982 GAAGAC BsiYI 123 CCACAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 502 CCGCGTCATGG BslI 123 CCACAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 502 CCGCGTCATGG BsmAI 1225 GAGAC GTCTC(1/5) 405 GTCTC BsrDI 651 CATTGC GCAATG(2/0) 364 GCAATG BssKI 63 CCAGG ^CC[ACGT]GG 999 CCAGG BstNI 63 CCAGG CC^[AT]GG 999 CCAGG BstYI 68 AGATCC [GA]^GATC[CT] 480 GGATCC DsaI 349 CCACGG C^C[GA][CT]GG 680 CCGTGG EcoRI 392 GAATTC G^AATTC 451 GAATTC EcoRII 63 CCAGG ^CC[AT]GG 999 CCAGG MaeII 284 ACGT A^CGT 419 ACGT MflI 68 AGATCC [GA]^GATC[CT] 480 GGATCC MspA1I 409 CAGCTG C[AC]G^C[GT]G 1084 CAGCTG MvaI 63 CCAGG CC^[AT]GG 999 CCAGG PstI 376 CTGCAG CTGCA^G 1026 CTGCAG PvuII 409 CAGCTG CAG^CTG 1084 CAGCTG RsaI 157 GTAC GT^AC 909 GTAC ScrFI 63 CCAGG CC^[ACGT]GG 999 CCAGG SmlI 39 CTTAAG C^T[CT][GA]AG 179 CTCAAG TfiI 3 GATTC G^A[AT]TC 586 GATTC XhoII 68 AGATCC [GA]^GATC[CT] 480 GGATCC XmnI 22 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 642 GAAAGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AcsI AluI AlwI ApoI AspHI AvaII BclI BmyI BsaJI BsiHKAI Bsp1286I BsrI BstF5I Cac8I Cfr13I DdeI DpnII DraII EcoO109I FbaI FokI HaeIII HinfI MboI MboII MnlI MseI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I SfcI Tru9I Tsp509I TspRI