################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BseAI BsgI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BsrI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII ClaI DdeI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeIII MamI MboII MfeI MluI MroI MseI MslI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 424 GACGTC GACGT^C AfaI 99 GTAC GT^AC ApaI 211 GGGCCC GGGCC^C AspHI 312 GAGCTC G[AT]GC[AT]^C BamHI 326 GGATCC G^GATCC BbrPI 106 CACGTG CAC^GTG BglI 250 GCCCGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 106 CACGTG [CT]AC^GT[GA] BsaWI 378 ACCGGA [AT]^CCGG[AT] BseRI 260 CTCCTC GAGGAG(10/8) BsiHKAI 312 GAGCTC G[AT]GC[AT]^C BsrBI 19 GAGCGG CCGCTC(-3/-3) BsrFI 38 GCCGGC [GA]^CCGG[CT] BssSI 276 CTCGTG CACGAG(-5/-1) BstYI 326 GGATCC [GA]^GATC[CT] Cfr10I 38 GCCGGC [GA]^CCGG[CT] CpoI 486 CGGACCG CG^G[AT]CCG DpnII 327 GATC ^GATC DraII 211 GGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 127 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 43 CCGCGG C^C[GA][CT]GG EcoO109I 211 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoT14I 462 CCTTGG C^C[AT][AT]GG HphI 172 TCACC GGTGA(8/7) KspI 43 CCGCGG CCGC^GG MboI 327 GATC ^GATC MflI 326 GGATCC [GA]^GATC[CT] NaeI 38 GCCGGC GCC^GGC NdeII 327 GATC ^GATC NgoMIV 38 GCCGGC G^CCGGC NlaIII 156 CATG CATG^ NotI 202 GCGGCCGC GC^GGCCGC PflMI 126 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 106 CACGTG CAC^GTG PmlI 106 CACGTG CAC^GTG RsaI 99 GTAC GT^AC RsrII 486 CGGACCG CG^G[AT]CCG SacI 312 GAGCTC GAGCT^C SacII 43 CCGCGG CCGC^GG Sau3AI 327 GATC ^GATC StyI 462 CCTTGG C^C[AT][AT]GG TspRI 53 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 126 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 326 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 327 GATCC GGATC(4/5) 326 GGATC AlwNI 286 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 362 CAGAGCCTG AvaII 444 GGACC G^G[AT]CC 487 GGACC BalI 218 TGGCCA TGG^CCA 405 TGGCCA BanI 8 GGCGCC G^G[CT][GA]CC 185 GGCGCC BbeI 8 GGCGCC GGCGC^C 185 GGCGCC BsiEI 40 CGGCCG CG[GA][CT]^CG 203 CGGCCG EagI 40 CGGCCG C^GGCCG 203 CGGCCG EclXI 40 CGGCCG C^GGCCG 203 CGGCCG Eco52I 40 CGGCCG C^GGCCG 203 CGGCCG HaeII 8 GGCGCC [GA]GCGC^[CT] 185 GGCGCC HinfI 114 GAGTC G^A[ACGT]TC 382 GAGTC KasI 8 GGCGCC G^GCGCC 185 GGCGCC MaeII 107 ACGT A^CGT 425 ACGT MluNI 218 TGGCCA TGG^CCA 405 TGGCCA MscI 218 TGGCCA TGG^CCA 405 TGGCCA NarI 8 GGCGCC GG^CGCC 185 GGCGCC PleI 114 GAGTC GAGTC(4/5) 382 GAGTC SfoI 8 GGCGCC GGC^GCC 185 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccII AciI AcyI AluI BanII BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I EaeI EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI