################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AluI 154 AGCT AG^CT Alw44I 277 GTGCAC G^TGCAC AlwNI 85 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 136 GGGCCC GGGCC^C ApaLI 277 GTGCAC G^TGCAC AvaII 96 GGACC G^G[AT]CC AviII 182 TGCGCA TGC^GCA BanII 136 GGGCCC G[GA]GC[CT]^C BbsI 424 GTCTTC GAAGAC(2/6) BglI 299 GCCGCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 424 GTCTTC GAAGAC(2/6) BsaI 400 GAGACC GGTCTC(1/5) BsiEI 222 CGACCG CG[GA][CT]^CG BsmAI 400 GAGAC GTCTC(1/5) BsrBI 337 GAGCGG CCGCTC(-3/-3) BsrDI 363 CATTGC GCAATG(2/0) BstF5I 186 CATCC GGATG(2/0) DraII 136 GGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 112 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 64 CCGCGG C^C[GA][CT]GG EaeI 297 TGGCCG [CT]^GGCC[GA] EcoNI 327 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 136 GGGCCCC [GA]G^G[ACGT]CC[CT] FokI 186 CATCC GGATG(9/13) FspI 182 TGCGCA TGC^GCA HaeII 57 GGCGCT [GA]GCGC^[CT] KspI 64 CCGCGG CCGC^GG MaeIII 323 GTTAC ^GT[ACGT]AC MboII 425 TCTTC GAAGA(8/7) NlaIII 312 CATG CATG^ PstI 350 CTGCAG CTGCA^G PvuII 153 CAGCTG CAG^CTG SacII 64 CCGCGG CCGC^GG SfcI 350 CTGCAG C^T[GA][CT]AG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 65 CGCG CG^CG 133 CGCG AspHI 277 GTGCAC G[AT]GC[AT]^C 284 GTGCTC BanI 110 GGCACC G^G[CT][GA]CC 307 GGCACC BsiHKAI 277 GTGCAC G[AT]GC[AT]^C 284 GTGCTC BsmFI 51 GGGAC GGGAC(10/14) 120 GTCCC BsrFI 41 GCCGGC [GA]^CCGG[CT] 204 GCCGGC BstAPI 174 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 352 GCAGGAGATGC BstUI 65 CGCG CG^CG 133 CGCG Cfr10I 41 GCCGGC [GA]^CCGG[CT] 204 GCCGGC DpnII 219 GATC ^GATC 237 GATC HphI 330 GGTGA GGTGA(8/7) 167 TCACC MboI 219 GATC ^GATC 237 GATC MspA1I 64 CCGCGG C[AC]G^C[GT]G 153 CAGCTG MvnI 65 CGCG CG^CG 133 CGCG NaeI 41 GCCGGC GCC^GGC 204 GCCGGC NdeII 219 GATC ^GATC 237 GATC NgoMIV 41 GCCGGC G^CCGGC 204 GCCGGC Sau3AI 219 GATC ^GATC 237 GATC SexAI 113 ACCTGGT A^CC[AT]GGT 326 ACCTGGT SfaNI 358 GATGC GCATC(5/9) 185 GCATC TaqI 221 TCGA T^CGA 398 TCGA TspRI 280 CACTG CA[GC]TG[ACGT][ACGT]^ 303 CACTG TthHB8I 221 TCGA T^CGA 398 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI BbvI BcnI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI TseI