Restriction map for
pFN21AE5072
(Circular) (Six-base) MAPSORT of: pFN21AE5072.seq Check: 564 from: 1 to: 5155
pFN21AE5072 5155 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2965 278
Size: 53 83 186 936 1429 2468
Fragments arranged by size:
2468 1429 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2498 1937
Size: 561 4594
Acc65I G'GTAC_C
Cuts at: 2476 2476
Size: 5155
AclI AA'CG_TT
Cuts at: 1563 3282 3655 1563
Size: 1719 373 3063
Fragments arranged by size:
3063 1719 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3218 4602 4870 1632
Size: 1586 1384 268 1917
Fragments arranged by size:
1917 1586 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4958
AflIII A'CryG_T
Cuts at: 1203 4054 5031 1203
Size: 2851 977 1327
Fragments arranged by size:
2851 1327 977
AgeI A'CCGG_T
Cuts at: 3986 3986
Size: 5155
AhdI GACnn_n'nnGTC
Cuts at: 3884 3884
Size: 5155
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5155
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5155
AlwNI CAG_nnn'CTG
Cuts at: 2095 2898 4470 2095
Size: 803 1572 2780
Fragments arranged by size:
2780 1572 803
ApaI G_GGCC'C
Cuts at: 2145 2145
Size: 5155
ApaLI G'TGCA_C
Cuts at: 2282 3212 4368 2282
Size: 930 1156 3069
Fragments arranged by size:
3069 1156 930
ApoI r'AATT_y
Cuts at: 1493 2464 2690 2842 4951 1493
Size: 971 226 152 2109 1697
Fragments arranged by size:
2109 1697 971 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3709 160
Size: 872 2677 1606
Fragments arranged by size:
2677 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5155
AvaI C'yCGr_G
Cuts at: 1870 1943 2480 1870
Size: 73 537 4545
Fragments arranged by size:
4545 537 73
BamHI G'GATC_C
Cuts at: 2485 2852 2485
Size: 367 4788
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2115 2312 2476 3931 618
Size: 325 217 627 328 197 164 1455 1842
Fragments arranged by size:
1842 1455 627 328 325 217 197 164
BanII G_rGCy'C
Cuts at: 1391 2145 2474 1391
Size: 754 329 4072
Fragments arranged by size:
4072 754 329
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2422 928
Size: 971 523 3661
Fragments arranged by size:
3661 971 523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3346 3380 1903
Size: 34 1409 34 3678
Fragments arranged by size:
3678 1409 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3047 4257 3047
Size: 1210 3945
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5155
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2310 3766 136
Size: 107 122 71 631 605 638 1456 1525
Fragments arranged by size:
1525 1456 638 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5150 5150
Size: 5155
BlpI GC'TnA_GC
Cuts at: 2572 2572
Size: 5155
Bme1580I G_kGCm'C
Cuts at: 1790 2145 2286 3216 4372 1790
Size: 355 141 930 1156 2573
Fragments arranged by size:
2573 1156 930 355 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5155
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2886 3844 442
Size: 416 53 83 399 24 1469 958 1753
Fragments arranged by size:
1753 1469 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3815 1239
Size: 2576 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3280 4145 4443 3280
Size: 865 298 3992
Fragments arranged by size:
3992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2399 3818 882
Size: 617 900 1419 2219
Fragments arranged by size:
2219 1419 900 617
BsaAI yAC'GTr
Cuts at: 493 1204 2946 493
Size: 711 1742 2702
Fragments arranged by size:
2702 1742 711
BsaBI GATnn'nnATC
Cuts at: 5149 5149
Size: 5155
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2962 3344 3979 275
Size: 53 83 186 936 1429 382 635 1451
Fragments arranged by size:
1451 1429 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3588 3986 4260 4407 4818 3588
Size: 398 274 147 411 3925
Fragments arranged by size:
3925 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2259 2289 1178
Size: 30 165 30 856 30 4044
Fragments arranged by size:
4044 856 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5155
BseYI C'CCAG_C
Cuts at: 4358 4854 4358
Size: 496 4659
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2162 2258 1163
Size: 573 297 129 96 4060
Fragments arranged by size:
4060 573 297 129 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2230 2605 3366 4394 1540
Size: 275 186 229 375 761 1028 2301
Fragments arranged by size:
2301 1028 761 375 275 229 186
BsiHKAI G_wGCw'C
Cuts at: 2286 2293 3216 3301 4372 2286
Size: 7 923 85 1071 3069
Fragments arranged by size:
3069 1071 923 85 7
BsmI GAATG_Cn'
Cuts at: 2666 2759 2666
Size: 93 5062
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2286 2293 2474 3216 3301 4372
Size: 399 355 141 7 181 742 85 1071
Cuts at: 4372 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 355 181 141 85
7
BspEI T'CCGG_A
Cuts at: 4818 4818
Size: 5155
BspHI T'CATG_A
Cuts at: 3044 4774 3044
Size: 1730 3425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2510 844
Size: 1666 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2344 3042 1394
Size: 429 521 698 3507
Fragments arranged by size:
3507 698 521 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2367 3650 3824 65
Size: 1183 1119 1283 174 1396
Fragments arranged by size:
1396 1283 1183 1119 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 2209 3799 3986 1700
Size: 110 236 163 1590 187 2869
Fragments arranged by size:
2869 1590 236 187 163 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5155
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5155
BssSI C'ACGA_G
Cuts at: 3215 4227 3215
Size: 1012 4143
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2185 2363 794
Size: 1391 178 3586
Fragments arranged by size:
3586 1391 178
BstBI TT'CG_AA
Cuts at: 3959 3959
Size: 5155
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5155
BstYI r'GATC_y
Cuts at: 1972 2485 2852 3237 3254 4706 4792 4804 5150
Size: 513 367 385 17 1452 86 12 346
Cuts at: 5150 1972
Size: 1977
Fragments arranged by size:
1977 1452 513 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2069 513
Size: 552 939 65 3599
Fragments arranged by size:
3599 939 552 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4416
Fragments arranged by size:
4416 683 56
BtsI GCAGTG_nn'
Cuts at: 2671 3465 3485 2671
Size: 794 20 4341
Fragments arranged by size:
4341 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5120
DraI TTT'AAA
Cuts at: 2459 2459
Size: 5155
DraIII CAC_nnn'GTG
Cuts at: 2122 2122
Size: 5155
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4162 809
Size: 507 2846 1802
Fragments arranged by size:
2846 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2602 3491
Size: 54 1425 50 275 18 472 300 889
Cuts at: 3491 5120 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 472 300 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2602 1537
Size: 275 790 4090
Fragments arranged by size:
4090 790 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3085 1353
Size: 1732 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3742 4116 4262 387
Size: 1064 138 2153 374 146 1280
Fragments arranged by size:
2153 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3218 3815 4602 4870 1239
Size: 393 1586 597 787 268 1524
Fragments arranged by size:
1586 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 2336
Size: 5155
EcoO109I rG'GnC_Cy
Cuts at: 2142 2142
Size: 5155
EcoRI G'AATT_C
Cuts at: 2464 2842 2464
Size: 378 4777
FspI TGC'GCA
Cuts at: 2189 3661 2189
Size: 1472 3683
HaeII r_GCGC'y
Cuts at: 1678 2066 4302 1678
Size: 388 2236 2531
Fragments arranged by size:
2531 2236 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3796 3828 3870 3902 1373
Size: 32 135 32 2224 32 42 32 2626
Fragments arranged by size:
2626 2224 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2499 2745 669
Size: 988 281 561 246 3079
Fragments arranged by size:
3079 988 561 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5155
HpaI GTT'AAC
Cuts at: 2745 2745
Size: 5155
KpnI G_GTAC'C
Cuts at: 2480 2480
Size: 5155
MfeI C'AATT_G
Cuts at: 2754 2754
Size: 5155
MluI A'CGCG_T
Cuts at: 5031 5031
Size: 5155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 2404 4269 4453 1305
Size: 774 325 1865 184 2007
Fragments arranged by size:
2007 1865 774 325 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3333
Fragments arranged by size:
3333 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3113 3472 3631 518
Size: 648 843 1104 359 159 2042
Fragments arranged by size:
2042 1104 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2160 2571 3248 4396 4641 1606
Size: 465 89 411 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 465 411 245 89
NaeI GCC'GGC
Cuts at: 2048 2211 2048
Size: 163 4992
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3664
Fragments arranged by size:
3664 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5155
NgoMIV G'CCGG_C
Cuts at: 2046 2209 2046
Size: 163 4992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5155
NotI GC'GGCC_GC
Cuts at: 2602 2602
Size: 5155
NspI r_CATG'y
Cuts at: 1132 1229 2513 4058 1132
Size: 97 1284 1545 2229
Fragments arranged by size:
2229 1545 1284 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5155
PciI A'CATG_T
Cuts at: 4054 4054
Size: 5155
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5155
PmeI GTTT'AAAC
Cuts at: 2459 2459
Size: 5155
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 4737 4769 3224
Size: 32 1481 32 3610
Fragments arranged by size:
3610 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5155
PsiI TTA'TAA
Cuts at: 2725 2725
Size: 5155
PspOMI G'GGCC_C
Cuts at: 2141 2141
Size: 5155
PstI C_TGCA'G
Cuts at: 830 2359 2507 830
Size: 1529 148 3478
Fragments arranged by size:
3478 1529 148
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5155
PvuII CAG'CTG
Cuts at: 2160 2160
Size: 5155
SacII CC_GC'GG
Cuts at: 2072 2072
Size: 5155
SalI G'TCGA_C
Cuts at: 1936 2497 1936
Size: 561 4594
SbfI CC_TGCA'GG
Cuts at: 2507 2507
Size: 5155
ScaI AGT'ACT
Cuts at: 3403 5115 3403
Size: 1712 3443
SexAI A'CCwGG_T
Cuts at: 2118 2331 2118
Size: 213 4942
SfcI C'TryA_G
Cuts at: 826 1045 2355 2503 3638 4319 4510 826
Size: 219 1310 148 1135 681 191 1471
Fragments arranged by size:
1471 1310 1135 681 219 191 148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5155
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5155
SmaI CCC'GGG
Cuts at: 1872 2482 1872
Size: 610 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3259 4160 4422 820
Size: 197 926 1316 901 262 1553
Fragments arranged by size:
1553 1316 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2946 493
Size: 2453 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5155
SphI G_CATG'C
Cuts at: 1132 2513 1132
Size: 1381 3774
SspI AAT'ATT
Cuts at: 5 52 3079 5
Size: 47 3027 2081
Fragments arranged by size:
3027 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5155
StyI C'CwwG_G
Cuts at: 513 1065 2004 2594 513
Size: 552 939 590 3074
Fragments arranged by size:
3074 939 590 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 3182 3199 3352 3537 3982 325
Size: 1255 443 1159 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1159 445 443 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3401 5113 96
Size: 274 80 33 51 1443 1424 1712 138
Fragments arranged by size:
1712 1443 1424 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2108 2140 2259 2291 5115 5147 2108
Size: 32 119 32 2824 32 2116
Fragments arranged by size:
2824 2116 119 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5155
XbaI T'CTAG_A
Cuts at: 2491 2491
Size: 5155
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5155
XmaI C'CCGG_G
Cuts at: 1870 2480 1870
Size: 610 4545
XmnI GAAnn'nnTTC
Cuts at: 3284 3284
Size: 5155
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2963 276
Size: 53 83 186 936 1429 2468
Fragments arranged by size:
2468 1429 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI