(Linear) (Six-base) MAPSORT of: KIEE5072.seq Check: 783 from: 1 to: 450 KIEE5072.orf With 182 enzymes: SgfI * December 5, 2014 12:59 .. AlwNI CAG_nnn'CTG Cuts at: 0 90 450 Size: 90 360 ApaI G_GGCC'C Cuts at: 0 140 450 Size: 140 310 ApaLI G'TGCA_C Cuts at: 0 277 450 Size: 277 173 BanI G'GyrC_C Cuts at: 0 110 307 450 Size: 110 197 143 Fragments arranged by size: 197 143 110 BanII G_rGCy'C Cuts at: 0 140 450 Size: 140 310 BbsI GAAGACnn'nnnn_ Cuts at: 0 417 450 Size: 417 33 BglI GCCn_nnn'nGGC Cuts at: 0 305 450 Size: 305 145 Bme1580I G_kGCm'C Cuts at: 0 140 281 450 Size: 140 141 169 Fragments arranged by size: 169 141 140 BsaI GGTCTCn'nnnn_ Cuts at: 0 394 450 Size: 394 56 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 254 284 450 Size: 254 30 166 Fragments arranged by size: 254 166 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 157 253 450 Size: 28 129 96 197 Fragments arranged by size: 197 129 96 28 BsiEI CG_ry'CG Cuts at: 0 225 450 Size: 225 225 BsiHKAI G_wGCw'C Cuts at: 0 281 288 450 Size: 281 7 162 Fragments arranged by size: 281 162 7 Bsp1286I G_dGCh'C Cuts at: 0 140 281 288 450 Size: 140 141 7 162 Fragments arranged by size: 162 141 140 7 BsrBI CCG'CTC Cuts at: 0 339 450 Size: 339 111 BsrDI GCAATG_nn' Cuts at: 0 362 450 Size: 362 88 BsrFI r'CCGG_y Cuts at: 0 41 204 450 Size: 41 163 246 Fragments arranged by size: 246 163 41 BstAPI GCAn_nnn'nTGC Cuts at: 0 180 358 450 Size: 180 178 92 Fragments arranged by size: 180 178 92 BtgI C'CryG_G Cuts at: 0 64 450 Size: 64 386 DraIII CAC_nnn'GTG Cuts at: 0 117 450 Size: 117 333 EaeI y'GGCC_r Cuts at: 0 297 450 Size: 297 153 EcoNI CCTnn'n_nnAGG Cuts at: 0 331 450 Size: 331 119 EcoO109I rG'GnC_Cy Cuts at: 0 137 450 Size: 137 313 FspI TGC'GCA Cuts at: 0 184 450 Size: 184 266 HaeII r_GCGC'y Cuts at: 0 61 450 Size: 61 389 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 74 399 450 Size: 74 325 51 Fragments arranged by size: 325 74 51 MspA1I CmG'CkG Cuts at: 0 66 155 450 Size: 66 89 295 Fragments arranged by size: 295 89 66 NaeI GCC'GGC Cuts at: 0 43 206 450 Size: 43 163 244 Fragments arranged by size: 244 163 43 NgoMIV G'CCGG_C Cuts at: 0 41 204 450 Size: 41 163 246 Fragments arranged by size: 246 163 41 PspOMI G'GGCC_C Cuts at: 0 136 450 Size: 136 314 PstI C_TGCA'G Cuts at: 0 354 450 Size: 354 96 PvuII CAG'CTG Cuts at: 0 155 450 Size: 155 295 SacII CC_GC'GG Cuts at: 0 67 450 Size: 67 383 SexAI A'CCwGG_T Cuts at: 0 113 326 450 Size: 113 213 124 Fragments arranged by size: 213 124 113 SfcI C'TryA_G Cuts at: 0 350 450 Size: 350 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 18 450 Size: 18 432 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 103 135 254 286 450 Size: 103 32 119 32 164 Fragments arranged by size: 164 119 103 32 32 Enzymes that do cut: AlwNI ApaI ApaLI BanI BanII BbsI BglI Bme1580I BsaI BsaXI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BstAPI BtgI DraIII EaeI EcoNI EcoO109I FspI HaeII MmeI MspA1I NaeI NgoMIV PspOMI PstI PvuII SacII SexAI SfcI TaqII TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI