(Circular) (Six-base) MAPSORT of: pFN21AE5064.seq Check: 8102 from: 1 to: 6367 pFN21AE5064 6367 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4177 278 Size: 53 83 186 936 2641 2468 Fragments arranged by size: 2641 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3710 1937 Size: 1773 4594 Acc65I G'GTAC_C Cuts at: 2023 3688 2023 Size: 1665 4702 AclI AA'CG_TT Cuts at: 1563 4494 4867 1563 Size: 2931 373 3063 Fragments arranged by size: 3063 2931 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2284 2389 3144 3393 4430 5814 6082 1632 Size: 652 105 755 249 1037 1384 268 1917 Fragments arranged by size: 1917 1384 1037 755 652 268 249 105 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6170 AflIII A'CryG_T Cuts at: 1203 3435 5266 6243 1203 Size: 2232 1831 977 1327 Fragments arranged by size: 2232 1831 1327 977 AgeI A'CCGG_T Cuts at: 5198 5198 Size: 6367 AhdI GACnn_n'nnGTC Cuts at: 2622 5096 2622 Size: 2474 3893 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6367 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6367 AlwNI CAG_nnn'CTG Cuts at: 4110 5682 4110 Size: 1572 4795 ApaLI G'TGCA_C Cuts at: 2134 2919 4424 5580 2134 Size: 785 1505 1156 2921 Fragments arranged by size: 2921 1505 1156 785 ApoI r'AATT_y Cuts at: 1493 2143 2349 2570 3676 3902 4054 6163 1493 Size: 650 206 221 1106 226 152 2109 1697 Fragments arranged by size: 2109 1697 1106 650 226 221 206 152 AseI AT'TA_AT Cuts at: 160 1032 4921 160 Size: 872 3889 1606 Fragments arranged by size: 3889 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6367 AvaI C'yCGr_G Cuts at: 1870 1943 3692 1870 Size: 73 1749 4545 Fragments arranged by size: 4545 1749 73 BamHI G'GATC_C Cuts at: 3697 4064 3697 Size: 367 6000 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 3688 5143 618 Size: 325 217 627 236 1665 1455 1842 Fragments arranged by size: 1842 1665 1455 627 325 236 217 BanII G_rGCy'C Cuts at: 1391 3686 1391 Size: 2295 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2649 3071 928 Size: 971 750 422 4224 Fragments arranged by size: 4224 971 750 422 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2999 3033 4558 4592 1903 Size: 34 1062 34 1525 34 3678 Fragments arranged by size: 3678 1525 1062 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4259 5469 4259 Size: 1210 5157 BclI T'GATC_A Cuts at: 1548 1548 Size: 6367 BfrBI ATG'CAT Cuts at: 2558 2558 Size: 6367 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4978 136 Size: 107 122 71 631 605 3306 1525 Fragments arranged by size: 3306 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6362 6362 Size: 6367 BlpI GC'TnA_GC Cuts at: 3784 3784 Size: 6367 Bme1580I G_kGCm'C Cuts at: 1790 2138 2923 4428 5584 1790 Size: 348 785 1505 1156 2573 Fragments arranged by size: 2573 1505 1156 785 348 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6367 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3501 4098 5056 Size: 416 53 83 399 24 2084 597 958 Cuts at: 5056 442 Size: 1753 Fragments arranged by size: 2084 1753 958 597 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6367 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3084 5027 1239 Size: 1845 1943 2579 Fragments arranged by size: 2579 1943 1845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2811 4492 5357 5655 2811 Size: 1681 865 298 3523 Fragments arranged by size: 3523 1681 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5030 882 Size: 617 3531 2219 Fragments arranged by size: 3531 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3436 4158 493 Size: 711 2232 722 2702 Fragments arranged by size: 2702 2232 722 711 BsaBI GATnn'nnATC Cuts at: 6361 6361 Size: 6367 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4174 4556 5191 275 Size: 53 83 186 936 2641 382 635 1451 Fragments arranged by size: 2641 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4800 5198 5472 5619 6030 4800 Size: 398 274 147 411 5137 Fragments arranged by size: 5137 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 3499 3529 1178 Size: 30 165 30 714 30 1352 30 4016 Fragments arranged by size: 4016 1352 714 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 3501 3504 1186 Size: 952 1363 3 4049 Fragments arranged by size: 4049 1363 952 3 BseYI C'CCAG_C Cuts at: 5570 6066 5570 Size: 496 5871 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3125 1163 Size: 573 1389 4405 Fragments arranged by size: 4405 1389 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3817 4578 5606 1540 Size: 275 186 1816 761 1028 2301 Fragments arranged by size: 2301 1816 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2138 2923 3355 4428 4513 5584 2138 Size: 785 432 1073 85 1071 2921 Fragments arranged by size: 2921 1073 1071 785 432 85 BsmI GAATG_Cn' Cuts at: 3878 3971 3878 Size: 93 6274 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2923 3355 3686 4428 4513 5584 Size: 399 348 785 432 331 742 85 1071 Cuts at: 5584 1391 Size: 2174 Fragments arranged by size: 2174 1071 785 742 432 399 348 331 85 BspEI T'CCGG_A Cuts at: 6030 6030 Size: 6367 BspHI T'CATG_A Cuts at: 4256 5986 4256 Size: 1730 4637 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3722 844 Size: 2878 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4254 1394 Size: 429 2431 3507 Fragments arranged by size: 3507 2431 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4862 5036 65 Size: 1183 3614 174 1396 Fragments arranged by size: 3614 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5011 5198 1700 Size: 110 3201 187 2869 Fragments arranged by size: 3201 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6367 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6367 BssSI C'ACGA_G Cuts at: 4427 5439 4427 Size: 1012 5355 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6367 BstBI TT'CG_AA Cuts at: 5171 5171 Size: 6367 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6367 BstXI CCAn_nnnn'nTGG Cuts at: 2530 2530 Size: 6367 BstYI r'GATC_y Cuts at: 1972 3416 3539 3697 4064 4449 4466 5918 6004 Size: 1444 123 158 367 385 17 1452 86 Cuts at: 6004 6016 6362 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1444 385 367 346 158 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3081 3081 Size: 6367 BtgI C'CryG_G Cuts at: 513 1065 2004 2544 2958 513 Size: 552 939 540 414 3922 Fragments arranged by size: 3922 939 552 540 414 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5628 Fragments arranged by size: 5628 683 56 BtsI GCAGTG_nn' Cuts at: 3883 4677 4697 3883 Size: 794 20 5553 Fragments arranged by size: 5553 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6367 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6332 DraI TTT'AAA Cuts at: 3671 3671 Size: 6367 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2240 5374 809 Size: 507 924 3134 1802 Fragments arranged by size: 3134 1802 924 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3814 4703 6332 Size: 54 1425 50 275 18 1984 889 1629 Cuts at: 6332 8 Size: 43 Fragments arranged by size: 1984 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3814 1537 Size: 275 2002 4090 Fragments arranged by size: 4090 2002 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4297 1353 Size: 2944 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2032 4954 5328 5474 387 Size: 1064 138 443 2922 374 146 1280 Fragments arranged by size: 2922 1280 1064 443 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2284 2389 3084 3144 3393 4430 5027 Size: 393 652 105 695 60 249 1037 597 Cuts at: 5027 5814 6082 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1037 787 695 652 597 393 268 249 105 60 EcoO109I rG'GnC_Cy Cuts at: 2210 2624 2210 Size: 414 5953 EcoRI G'AATT_C Cuts at: 3676 4054 3676 Size: 378 5989 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3258 3258 Size: 6367 FspI TGC'GCA Cuts at: 4873 4873 Size: 6367 HaeII r_GCGC'y Cuts at: 1678 5514 1678 Size: 3836 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2608 2640 2809 2841 5008 Size: 32 135 32 1036 32 169 32 2167 Cuts at: 5008 5040 5082 5114 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2167 1036 169 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3025 3711 3957 669 Size: 988 281 1087 686 246 3079 Fragments arranged by size: 3079 1087 988 686 281 246 HindIII A'AGCT_T Cuts at: 748 2453 748 Size: 1705 4662 HpaI GTT'AAC Cuts at: 3957 3957 Size: 6367 KpnI G_GTAC'C Cuts at: 2027 3692 2027 Size: 1665 4702 MfeI C'AATT_G Cuts at: 3966 3966 Size: 6367 MluI A'CGCG_T Cuts at: 6243 6243 Size: 6367 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3394 5481 5665 1305 Size: 2089 2087 184 2007 Fragments arranged by size: 2089 2087 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4545 Fragments arranged by size: 4545 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4325 4684 4843 518 Size: 648 3159 359 159 2042 Fragments arranged by size: 3159 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3095 3329 3783 4460 5608 5853 1606 Size: 1489 234 454 677 1148 245 2120 Fragments arranged by size: 2120 1489 1148 677 454 245 234 NcoI C'CATG_G Cuts at: 513 1065 2004 2544 2958 513 Size: 552 939 540 414 3922 Fragments arranged by size: 3922 939 552 540 414 NdeI CA'TA_TG Cuts at: 387 387 Size: 6367 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6367 NotI GC'GGCC_GC Cuts at: 3814 3814 Size: 6367 NsiI A_TGCA'T Cuts at: 2560 2560 Size: 6367 NspI r_CATG'y Cuts at: 1132 1229 2562 3725 5270 1132 Size: 97 1333 1163 1545 2229 Fragments arranged by size: 2229 1545 1333 1163 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6367 PciI A'CATG_T Cuts at: 5266 5266 Size: 6367 PflMI CCAn_nnn'nTGG Cuts at: 1108 2964 3536 1108 Size: 1856 572 3939 Fragments arranged by size: 3939 1856 572 PfoI T'CCnGG_A Cuts at: 2157 2197 2157 Size: 40 6327 PmeI GTTT'AAAC Cuts at: 3671 3671 Size: 6367 PmlI CAC'GTG Cuts at: 3436 3436 Size: 6367 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4436 4468 5949 5981 4436 Size: 32 1481 32 4822 Fragments arranged by size: 4822 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2210 2624 2210 Size: 414 5953 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6367 PsiI TTA'TAA Cuts at: 3937 3937 Size: 6367 PstI C_TGCA'G Cuts at: 830 3138 3346 3456 3719 830 Size: 2308 208 110 263 3478 Fragments arranged by size: 3478 2308 263 208 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6367 PvuII CAG'CTG Cuts at: 3329 3329 Size: 6367 SalI G'TCGA_C Cuts at: 1936 3709 1936 Size: 1773 4594 SbfI CC_TGCA'GG Cuts at: 3719 3719 Size: 6367 ScaI AGT'ACT Cuts at: 4615 6327 4615 Size: 1712 4655 SfcI C'TryA_G Cuts at: 826 1045 3134 3342 3452 3715 4850 5531 5722 Size: 219 2089 208 110 263 1135 681 191 Cuts at: 5722 826 Size: 1471 Fragments arranged by size: 2089 1471 1135 681 263 219 208 191 110 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6367 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6367 SmaI CCC'GGG Cuts at: 1872 3694 1872 Size: 1822 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2826 4471 5372 5634 820 Size: 197 926 883 1645 901 262 1553 Fragments arranged by size: 1645 1553 926 901 883 262 197 SnaBI TAC'GTA Cuts at: 493 4158 493 Size: 3665 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6367 SphI G_CATG'C Cuts at: 1132 2562 3725 1132 Size: 1430 1163 3774 Fragments arranged by size: 3774 1430 1163 SspI AAT'ATT Cuts at: 5 52 3040 4291 5 Size: 47 2988 1251 2081 Fragments arranged by size: 2988 2081 1251 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6367 StyI C'CwwG_G Cuts at: 513 1065 2004 2544 2958 3806 513 Size: 552 939 540 414 848 3074 Fragments arranged by size: 3074 939 848 552 540 414 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3270 4394 4411 4564 4749 5194 325 Size: 1255 1690 1124 17 153 185 445 1498 Fragments arranged by size: 1690 1498 1255 1124 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4613 6325 96 Size: 274 80 33 51 1443 2636 1712 138 Fragments arranged by size: 2636 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2149 6327 6359 2117 Size: 32 4178 32 2125 Fragments arranged by size: 4178 2125 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6367 XbaI T'CTAG_A Cuts at: 3703 3703 Size: 6367 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2530 3043 2530 Size: 513 5854 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6367 XmaI C'CCGG_G Cuts at: 1870 3692 1870 Size: 1822 4545 XmnI GAAnn'nnTTC Cuts at: 2856 3229 3406 4496 2856 Size: 373 177 1090 4727 Fragments arranged by size: 4727 1090 373 177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4175 276 Size: 53 83 186 936 2641 2468 Fragments arranged by size: 2641 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI